FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008104496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008104496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287405
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACAAAATGTTTCACTTTTGGGTAATACGGTGCAGATAATGACAAGGA133004.627616081835737No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA128374.466519371618448No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC87643.0493554391886017No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG86182.9985560446060435No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACCGTAGTGCTAAAATGGA81982.8524207999164943No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA75682.6332179328821694No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG69402.4147109479654145No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACGTCGTAGGGACGAACTGGTGT65752.2877124615090203No Hit
TGTGCTGTGACTGCTTGTAGAAACAGAGTTGAAAGTCAGGGCAAGACCAA56861.9783928602494738No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT55631.935596110018963No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG55451.9293331709608392No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA55031.914719646491884No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG52261.8183399732085386No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC51581.7946799812111829No Hit
ACTTCTACCGTGCCCTGAGGAAGCTGTGTCTACTCATTTGAACAGACCAA51521.7925923348584751No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGTCATGCGTCAT45461.581740053234982No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA44251.5396391851220403No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT36861.28251074268019No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTATGTGGGGGTTG36681.2762478036220664No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGACCAAGCTGTGTCTACTCATTT33781.1753448965745201No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG32501.1308084410500863No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCAGTGTTGTGGGGGTTGTTGACTT28951.0072893651815382No Hit
GCAGCTTCTGCCATCTCTCTGCTACCGTAAGAACGTGGGAGTAGACGGAA28801.0020702492997686No Hit
GGGGTCAGAGGCAAGCAGACCAAGGGAGATAACACAGGCCCAAGAGACCA27990.9738870235382125No Hit
AGGACAAAATGTTTCACTTTTGGGTACGGTGCAGATAATGACAAGGAATA27820.9679720255388737No Hit
TCATAATGCTTGCTCTGATAGGAACACTGACGACATGGAGTGGGTCCCAT26970.9383970355421791No Hit
GTAGAGGAGCCGTCAAATCCAGAGGGATAAAACACCATGAAAGACCAAGT25580.890033228371114No Hit
GGTCTTCTCTGTTTCAGGGCATGGTAGAAGTTGTAGGAGGTGGCTTTAGG23460.81626972390877No Hit
GTAGAGGAGCCGTCAAATCAAGAGGGATAAAACACCATGAAAGACCAAGT22370.7783441485012439No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT21410.7449418068579182No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG20540.7146709347436544No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT20000.6958821175692838No Hit
CCCCTGCTCCTATAGCCAAGTCGATGCCGACGAGTACCTCATAGACCAAG18940.6590003653381117No Hit
CTGGTGTTGTTGGGCAGTGTCTCCCCTTTCTTGCGGAGATAGACCAAGTC18860.6562168368678346No Hit
AGATGAGTCATATTTGTGGGTTTTCACCACTGACTCTGATCCAGAAGACC18380.6395156660461717No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGGCTAATGAGTTTGTACTGAGG18260.6353403733407561No Hit
TCTTAAATGGCTACGACCCAGCACTGACGACATGGCTACCTGTTAAAGAA17760.6179433204015239No Hit
CCCCTGCTCCTATAGCCAAGTCTGGATGCCGACGAGTACCTCATAGACCA16670.580017744993998No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGGCTAATGAGTTTGTA15010.5222595292357475No Hit
GCAGCTTCTGCCATCTCTCTGCTACCGTAGAACGTGGGAGTAGACGGAAG15000.5219115881769628No Hit
GTGTCACTCGTAATTAGGTCCAGACCATCTCTGCTACCGTAGCTAATGAG14230.4951201266505454No Hit
ATACGGCCAGGCATTGAAGTCAGAGCAGTCAGAGCAGTCAGAGGACCAGG14040.48850924653363714No Hit
TCAAATCATCCATTGCTTGGTGCAGAAAGTCAGTCCCATGGAGACCAAGT13570.472156016770759No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA13440.46763278300655864No Hit
GACCCAAAACCCAAAATGGCACAGTAGGAGACAGAAGCAGGGAAGACCAA13140.45719455124301944No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC12790.445016614185557No Hit
GGGGTCAGAGGCAAGCAGTCACAGCACATGTAGAACCAGGAGATAACACA12530.43597014665715633No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTAGTC12510.435274264539587No Hit
GGAATCCTATGGCTTTCCAACCTGGTTCTACAGCAACAGAGGAGGGGGAG11770.4095266261895235No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC11700.407091038778031No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAG11430.39769663019084567No Hit
TCTTAAATGGCTACGACCCAGGACCAGAGGAAACAGACCAAGCTACCTGT11330.39421721960299927No Hit
TCCCTGCCAGCGAGATACACTGACGACATGGTTCTACATACCCTCCATGA11110.38656251630973715No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA10460.36394634748873544No Hit
TCATAATGCTTGCTCTGATAGGACACTGACGACATGGAGTGGGTCCCATC9690.33715488596231796No Hit
TGAGCAAGAGGCTTTGGAGTTGGTTCTACTCTCAGCAGGCAAAGACCAGA9530.3315878290217637No Hit
GCAACAGATAACTCAGATTGCCTTTCTACTATTGCAGTCTGTGCTTCCAT9070.3155825403176702No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC8180.28461578608583704No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTAGTCATGCGTCATCTCG7720.26861049738174353No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGCATTGAAGT7670.26687079208782033No Hit
GGGGTCAGAGGCAAGCAGTGTGGAGATAACACAGGCCCAAGAGACCAAGT7500.2609557940884814No Hit
GGTCTTCTCTGTTTCAGGGCATCTTCTTACCCAATCCCATGAGTGGGGAG7240.25190932656008075No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTATTC7150.24877785703101896No Hit
TCCCTGCCAGCGAGATGAGGCCTTTGCATCCCTAGACCAAGTCTCTGCTA5740.19971816774238443No Hit
TCAAATCATCCATTGCTTGGTACGGTAGCAGAAAGTCAGTCCCATGGAGA5310.18475670221464482No Hit
AGCTGCTCACCATCGCTACTGTAGCAGGTCTTGGCCAGTTGGAGACCAAG4870.1694472956281206No Hit
ATACGGCCAGGCATTGAAGTTATAGCAGAGCAGTCAGAGGACCAGGAGAC4680.16283641551121242No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTAGTC4500.15657347645308886No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTAGTCATGCGTCATCT4320.1503105373949653No Hit
GGAATCCTATGGCTTTCCAACCATTACGGCAGCAACAGAGGAGGGGGAGA4280.14891877315982674No Hit
TCAAATCATCCATTGCTTGGTACGGGTAGCAGAAAGTCAGTCCCATGGAG4170.14509142151319568No Hit
GACCCAAAACCCAAAATGGTACGGTAGGAGACAGAAGCAGGGAAGACCAA4110.14300377516048782No Hit
ATACGGCCAGGCATTGAAGTTCACTGACGACAGGGGCCAGGAGGAGACCA3820.1329134844557332No Hit
ATACGGCCAGGCATTGAAGTCTCTGCTACCGTAAGACAGGGGCCAGGAGG3760.13082583810302534No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT3710.12908613280910214No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAATACGGCC3690.12839025069153284No Hit
GGTCTTCTCTGTTTCAGGGCAGACCAAGTCTCTGCTCGTAGGAGGTGGCT3320.11551643151650111No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA3220.1120370209286547No Hit
GAGAAAGCCCCCCTACTGCACACTGACGACATGTTCTACAGAGAAAGCCC3120.10855761034080827No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGCTACCGTAGTCATGC3100.10786172822323899No Hit
TGAGCAAGAGGCTTTGGAGTACACTGACGACTCTCAGCAGGCAAAGACCA2950.10264261234146936No Hit
GGAAACCGTAGCTGCCCTGGGTTCATCTGGACCTGGGTCTTAGACCAAGT2950.10264261234146936No Hit
ATACGGCCAGGCATTGAAGTACGCTGACGACAGGGGCCAGGAGGAGACCA2910.1012508481063308No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)