FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008104497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008104497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287405
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA138244.80993719663889No Hit
ACTAGATATTCCTTGTCATTATCTGCACCGTATTACCCAAAAGTGAAACA132384.606043736191089No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA88243.07023190271568No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG85322.9686331135505646No Hit
AACAGAGAATCTCCATTTTAGCACTACGGTAGCAGAGACTTTGTCATATT83732.9133104852038065No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA76332.6558341017031717No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA68912.3976618360849673No Hit
TCATTACACCAGTTCGTCCCTACGACGTGGTAAAAAGATAACCTCAGAAT64952.2598771768062487No Hit
TGCCCTGACTTTCAACTCTGTTTCTACAAGCAGTCACAGCACATGTAGAA56921.9804805066021816No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT55541.932464640489901No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT54961.9122840590803918No Hit
GTTCAAATGAGTAGACACAGCTTCCTCAGGGCACGGTAGAAGTTGTAGAA51861.8044223308571528No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA51331.7859814547415667No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG51091.7776308693307354No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG46751.626624449818201No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT44631.5528609453558568No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC36941.2852942711504671No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA36871.2828586837389746No Hit
ACAAGTCAACAACCCCCACATACGGTAGCAGAGACTTCCATTTTCTACTT34631.2049198865712147No Hit
GTTCAAATGAGTAGACACAGCTTGGTCTTCTCTGTTTCAGGGCACGGTAG33991.182651658808998No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG32501.1308084410500863No Hit
TCCGTCTACTCCCACGTTCTTACGGTAGCAGAGAGATGGCAGAAGCTGCT28700.9985908387119222No Hit
ACAAGTCAACAACCCCCACAACACTGACTTCCATTTTCTACTTTTTCTGA28530.9926758407125832No Hit
CTTGGGCCTGTGTTATCTCCCTTGGTCTGCTTGCCTCTGACCCCTGTAGA28220.9818896678902594No Hit
ACTAGATATTCCTTGTCATTATCTGCACCGTACCCAAAAGTGAAACATTT27900.9707555540091508No Hit
CTGATGGGACCCACTCCATGTCGTCAGTGTTCCTATCAGAGCAAGCATTA26620.9262190984847167No Hit
TTCATGGTGTTTTATCCCTCTGGATTTGACGGCTCCTCTACTGTAGAACC25380.8830744071954212No Hit
GCTGACCTAAAGCCACCTCCTACAACTTCTACCATGCCCTGAAACAGAGA23710.824968250378386No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG22300.7759085610897514No Hit
TTCATGGTGTTTTATCCCTCTTGATTTGACGGCTCCTCTACTGTAGAACC22180.7717332683843358No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG20910.7275447539186862No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT20230.7038847619213305No Hit
ATGAGGTACTCGTCGGCATCGACTTGGCTATAGGAGCAGGGGTGTAGAAC19160.6666550686313739No Hit
ATCTCCGCAAGAAAGGGGAGACACTGCCCAACAACACCAGTGTAGAACCA18920.6583044832205424No Hit
TCTGGATCAGAGTCAGTGGTGAAAACCCACAAATATGACTCATCTTGTAG18340.6381239018110332No Hit
GGCCTCAGTACAAACTCATTAGCCTTGGTCTGGACCTAATTACGAGTGAC18280.6360362554583254No Hit
TCCAGATGATTCTTTAACAGGTAGCCATGTCGTCAGTGCTGGGTCGTAGC17240.5998503853447226No Hit
ATGAGGTACTCGTCGGCATCCAGACTTGGCTATAGGAGCAGGGGTGTAGA16750.5828012734642751No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT15340.5337415841756407No Hit
TCCGTCTACTCCCACGTTCTACGGTAGCAGAGAGATGGCAGAAGCTGCTG14910.5187801186479011No Hit
GGCCTCAGTACAAACTCATTAGCCAGAGACTTGGTCTGGACCTAATTACG14880.5177362954715471No Hit
GGCCTCAGTACAAACTCATTAGCTACGGTAGCAGAGATGGTCTGGACCTA14010.48746542335728327No Hit
CCTGGTCCTCTGACTGCTCTGACTGCTCTGACTTCAATGCCTGGCCGTAT14000.48711748229849866No Hit
AGGGATGCAAAGGCCTCATGGAGGGTATGTAGAACCATGTCGTCAGTGTA13920.48433395382822153No Hit
CCATGGGACTGACTTTCTGCACCAAGCAATGGATGATTTGATGTAGAACC13630.4742436631234669No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA13590.47285189888832835No Hit
TCCCTGCTTCTGTCTCCTACTGTGCCATTTTGGGTTTTGGGTCTGTAGAA13050.4540630817139577No Hit
CTTGGGCCTGTGTTATCTCCTGGTTCTACATGTGCTGTGACTGCTTGCCT12960.4509316121848959No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA12580.4377098519510795No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC12380.4307510307753866No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG12320.4286633844226788No Hit
CCCCCTCCTCTGTTGCTGTAGAACCAGGTTGGAAAGCCATAGGATTCCTG11920.4147457420712931No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA11670.40604721560167706No Hit
TCCAGATGATTCTTTAACAGGTAGCTTGGTCTGTTTCCTCTGGTCCTGGG11320.3938692785442146No Hit
GGTCTTTGCCTGCTGAGAGTAGAACCAACTCCAAAGCCTCTTGCTCATGT9610.33437135749204083No Hit
CTGATGGGACCCACTCCATGTCGTCAGTGTCCTATCAGAGCAAGCATTAT9580.33332753431568696No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA9480.3298481237278405No Hit
CAATGGAAGCACAGACTGCAATAGTAGAAAGGCAATCTGAGTTATCTGTT9030.3141907760825316No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC8800.30618813173048487No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC8110.2821801986743446Illumina Single End PCR Primer 1 (100% over 21bp)
CTTGGGCCTGTGTTATCTCCACACTGCTTGCCTCTGACCCCTGTAGAACC7370.2564325603242811No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCCCTGTCGTCA7350.25573667820671175No Hit
CCATGGGACTGACTTTCTGCTCTTTTCACCCATCTACAGTCCCCCTTGCC7180.2498216802073729No Hit
GCTGACCTAAAGCCACCTCCCCACTCATGGGATTGGGTAAGAAGATGCCC7090.2466902106783111No Hit
AGGGATGCAAAGGCCTCATCTCGCTGGCAGGGATGTAGAACCATGTCGTC6420.2233781597397401No Hit
CCATGGGACTGACTTTCTGCTACCGTACCAAGCAATGGATGATTTGATGT5400.18788817174370662No Hit
CCAACTGGCCAAGACCTGCTACAGTAGCGATGGTGAGCAGCTTGTAGAAC4910.17083905986325915No Hit
CCTGGTCCTCTGACTGCTCTGCTATAACTTCAATGCCTGGCCGTATTGTA4720.16422817974635098No Hit
CCTGGTCCTCTGACTGCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGA4700.1635322976287817No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC4680.16283641551121242No Hit
CCCCCTCCTCTGTTGCTGCCGTAATGGTTGGAAAGCCATAGGATTCCTGT4340.15100641951253457No Hit
CCATGGGACTGACTTTCTGCTACCCGTACCAAGCAATGGATGATTTGATG4190.14578730363076495No Hit
TCCCTGCTTCTGTCTCCTACCGTACCATTTTGGGTTTTGGGTCTGTAGAA4110.14300377516048782No Hit
CCTCCTGGCCCCTGTCTTACGGTAGCAGAGACTTCAATGCCTGGCCGTAT3920.13639289504357963No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC3910.13604495398479496No Hit
CCTCCTGGCCCCTGTCGTCAGTGAACTTCAATGCCTGGCCGTATTGTAGA3860.13430524869087176No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT3820.1329134844557332No Hit
GGACCTAATTACGAGTGACACTGTAGAACCATGTCGTCAGTGTAGATCTC3270.1137767262225779No Hit
GCTGACCTAAAGCCACCTCCTACGAGCAGAGACTTGGTCTGCCCTGAAAC3270.1137767262225779No Hit
CCTCCTGGCCCCTGTCGTCAGCGTACTTCAATGCCTGGCCGTATTGTAGA3070.10681790504688506No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA3030.10542614081174649No Hit
GGTCTTTGCCTGCTGAGAGTCGTCAGTGTACTCCAAAGCCTCTTGCTCAT2920.10159878916511543No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)