FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008104504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008104504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370242
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC309588.361558116043021No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA193945.238195558580604No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC144253.8961003883946175No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT113743.0720447707175307No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA96712.612075345314686No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG85202.3011975950864567No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGAACCA80042.161829290031925No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTACTGAGAAACCGTGTGAAATTG66971.8088169359500004No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACAGTCAGAGTAT61191.652702826799769No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC59951.6192112186083696No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT54211.4641774839159252No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA51841.4001652972920415No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC50921.3753166847629388No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA49611.3399344212704123No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC46571.257825962478595No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA45831.2378390350095343No Hit
GACCCAAAACCCAAAATGGCGCTGGGATAATTGGCTAGCCACACGTAGGA44691.2070483629626028No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA44521.2024567715170078No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTATGTGGGGGTTG42331.1433062699531658No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTTATACCAGGACCA35520.9593725185149173No Hit
CAGAGGGGAAAAATATGACAAAATTAAGGTGCTAAAATGGAGATTCTCTG35450.9574818632137898No Hit
AGGACAAAATGTTTCACTTTTGGGTAATACGGTGCAGATAATGACAAGGA32820.8864472426142901No Hit
ACTTCTACCGTGCCCTGCTACCGTAAGCTGTGTCTACTCATTTGAACAGA32460.8767238724942065No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG31690.8559266641818055No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG28860.7794901712933703No Hit
TGTGGTCTGCCAGCTAAAGGTTTGTACTTTACTTTCATTGGGAGAAGACC26100.7049443337060625No Hit
TGACCAATGGCTAAGTGAAGATGAGTCATATTTGTGTTTTTCCTTTCCTC25990.7019733039471481No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC25280.6827966573214276No Hit
GCAGCTTCTGCCATCTCTCTGCTACCGTAAGAACGTGGGAGTAGACGGAA25200.6806359084058535No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT23680.6395816790099449No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG23040.6222956876853518No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC22900.618514377083097No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGAAGCTGTGTCTACTCATTTGAA21080.5693573392537853No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG19760.5337049821468121No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC19680.5315442332312379No Hit
TCAAATCATCCATTGCTTGGACGGAGCAGAAAGTCAGTCCCATGGAGACC19140.5169591780511125No Hit
AGAAAACGGCATTTTGAGTGTCAGTGTTGAGGCATAACTGCACCCTTAGA19080.5153386163644319No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT18410.4972423441964985No Hit
TCTTAAATGGCTACGACCCAGTCTACACTGACGACATGTTCTACATCTTA17190.4642909232339929No Hit
CAGAGGGGAAAAATATGACAAAGGTGCTAAAATGGAGATTCTCTGTTAGA15580.4208058513080634No Hit
GCAACAGATAACTCAGATTGCCTTGTCTTTTCCTTCCTAACAAAACCTCA14840.40081892383900264No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG14650.39568714516451403No Hit
ACTTCTACCGTGCCCTGATACGGTAAGCTGTGTCTACTCATTTGAACAGA14060.3797516219121548No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCAAGGTGGGGGCAGCGCCT13530.3654366603464761No Hit
CTGGTGTTGTTGGGCAGTTCTATCTCCCCTTTCTTGCGGAGATAGACCAA13510.36489647311758255No Hit
CAGCCTCTGGCATTCTGGCTGAGACCAAGTCTAGAGCAGTCAGAGGACCA13440.3630058178164552No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACCGGTGCTAAAATGGAGA13150.35517310299749894No Hit
TCATAATGCTTGCTCTGATAGGAACACTGACGACATGGAGTGGGTCCCAT12450.33626654998622524No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTACAGTCAGAGTATCTCG12190.32924411601060927No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT11970.3233020564927804No Hit
TCCCTGCCAGCGAGATGAGGCCTTTGCATCCCTAGACCAAGTCTCTGCTA11930.32222168203499335No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG11820.3192506522760789No Hit
CAGTCAGAGGCGCTATGTGTGGAACAGTTTTCTTAACCATATCTAGAAGA11590.3130384991438032No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTATACGGAAGGCAGTCTGGCTG11550.3119581246860162No Hit
GTGTCACTCGTAATTAGGTCCAAGTCTCTGCTACGCTAATGAGTTTGTAC11260.30412540986705994No Hit
GCAACAGATAACTCAGATTGCCTTTCCTCATACCAGGACCAGAGGAAACA10980.2965627886625505No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAC10980.2965627886625505No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG10910.29467213336142306No Hit
CAGAGGGGAAAAATATGACAAAGTGTGCTAAAATGGAGATTCTCTGTTAG10810.29197119721695536No Hit
TCACAATTGCCAGTTAACGTTTCTGCTTTGCTGTGTGGAGACCAAGTCTC10560.2852188568557862No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTACAG9700.2619908060133642No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC9630.26010015071223686No Hit
AGAAAACGGCATTTTGAGTGTTCTAAGGGTGAGGCATAACTGCACCCTTA9070.2449749083032179No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTGTGGGGGTTGTTGACTTGTAGA8970.2422739721587502No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT8900.24038331685762288No Hit
GGGGTCAGAGGCAAGCAGGCCCAAGAGATAACACAGGCCCAAGAGATAAC8480.22903938505085863No Hit
TCAAATCATCCATTGCTTGGATGGTAGCAGAAAGTCAGTCCCATGGAGAC7860.21229358095515904No Hit
TCCCTGCCAGCGAGATACACTGAGGCCTTTGCATCCCTAGACCAAGTCTC7740.20905245758179786No Hit
GACCCAAAACCCAAAATGGCATTGCTTAAAGAAGGCTGTAGGAGACAGAA7290.19689824493169333No Hit
TGAGCAAGAGGCTTTGGAGTACACTGACGACACTCTCAGCAGGCAAAGAC7110.19203655987165152No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCAGTGTTGTGGGGGTTGTTGACTT7060.19068609179941767No Hit
TCTTAAATGGCTACGACCCAGACACTGACGACATGGTTCTACATCTTAAA6680.1804225344504405No Hit
CAGCCTCTGGCATTCTGGTCGGTAGCAGAGCAGTCAGAGGACCAGGAGAC6520.17610103661929224No Hit
CCCCTGCTCCTATAGCCAAGTCGTCAGTGTGATGCCGACGAGTACCTCAT6450.17421038131816488No Hit
TCCACAAACAGAACAAGATGCTACCGTAAATCCTGTTTGAAGGAAAAGGC6420.17340010047482457No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT6350.1715094451736972No Hit
GCAGCTTCTGCCATCTCTCTGCTACAGAACGTGGGAGTAGACGGAAGACC6180.16691785372810217No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA6170.1666477601136554No Hit
GGGGTCAGAGGCAAGCAGGCAGGACATGTAGAACCATGGGAGATAACACA6160.16637766649920863No Hit
GGAATCCTATGGCTTTCCAACCACATGGTTCTACAGCAACAGAGGAGGGG6000.16205616866806033No Hit
TGTGGTCTGCCAGCTAAAGGGTTTGTACTTTACTTTCATTGGGAGAAGAC5900.15935523252359268No Hit
AGATGAGTCATATTTGTGGGTTTTCACCACTGACTCTGATCCAGAAGACC5810.15692438999357178No Hit
GGTCTTCTCTGTTTCAGGGCATCTGTAATCCCAGCTACTCAGGAGGTGGC5800.156654296379125No Hit
TCATAATGCTTGCTCTGATAGGAACACTGGACGACATGGAGTGGGTCCCA5800.156654296379125No Hit
TCACAATTGCCAGTTAACGTCTCTGCTTTGCTGTGTGGAGACCAAGTCTC5740.15503373469244439No Hit
GTAGAGGAGCCGTCAAATCCATGAAAGACCAAGTCTCTGCTACCGTACAG5740.15503373469244439No Hit
GGGGTCAGAGGCAAGCAGGGAGATAACACAGGCCCAAGAGACCAAGTCTC5630.15206270493352997No Hit
GGGGTCAGAGGCAAGCAGGCCCAGGAGATAACACAGGCCCAAGAGACCAA5210.14071877312676573No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGCTACCGTACAGTCAG5090.13747764975340454No Hit
AGCTGCTCACCATCGCTACCCGTAAGGTCTTGGCCAGTTGGAGACCAAGT5060.13666736891006423No Hit
GGGGTCAGAGGCAAGCAGTGTCGTCAGTGGGAGATAACACAGGCCCAAGA4930.13315615192225624No Hit
GGGGTCAGAGGCAAGCAGGAGATAACACAGGCCCAAGAGACCAAGTCTCT4520.1220823137299388No Hit
ATACGGCCAGGCATTGAAGTCACTGACGACAGGGGCCAGGAGGAGACCAA4400.11884119035657759No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGGCTAATGAGTTTGTACTGAGG4380.11830100312768405No Hit
TCCCTGCCAGCGAGATGGATGAGGCCTTTGCATCCCTAGACCAAGTCTCT4330.11695053505545022No Hit
GGTCTTCTCTGTTTCAGGGCATGGTAGAAGTTGGGAGGTGGCTTTAGGTC4190.11316922445319547No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACAAGTCTCTGCTACCGTATTCA4020.10857763300760044No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTACAG3810.10290566710421832No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTACAGTCAGAGTATCT3790.1023654798753248No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACAGTCAGAGTAT3750.10128510541753773No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)