FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008104505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008104505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370242
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC303388.194100075086025No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA297588.0374457787069No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG138133.730803096353196No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA97802.641515549289384No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA85562.310920965206541No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA85352.3052489993031586No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG62981.7010495837857402No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCAATTTCACACGGTTTCTCAGT62911.6991589284846127No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA60371.630555150415134No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC54201.4639073903014785No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA52261.4115092290988056No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC51081.379638182594087No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA47161.2737614857309545No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT46221.2483726859729583No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT44901.212720328865985No Hit
TCCCTGCTTCTGTCTCCTACGTGTGGCTAGCCAATTATCCCAGCGCCATT44781.2094792054926238No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT43771.1821997504335002No Hit
ACAAGTCAACAACCCCCACATACGGTAGCAGAGACTTCCATTTTCTACTT40831.1027922277861508No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG36210.9780089779117442No Hit
AACAGAGAATCTCCATTTTAGCACCTTAATTTTGTCATATTTTTCCCCTC35540.9599127057438108No Hit
ACTAGATATTCCTTGTCATTATCTGCACCGTATTACCCAAAAGTGAAACA32800.8859070553853966No Hit
GTTCAAATGAGTAGACACAGCTTACGGTAGCAGGGCACGGTAGAAGTTGT32400.8751033108075259No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT31930.8624089109285279No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC27570.7446480950297373No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTAGCTGGCAGACCACATGTAG26140.7060247081638495No Hit
TCCGTCTACTCCCACGTTCTTACGGTAGCAGAGAGATGGCAGAAGCTGCT25290.6830667509358744No Hit
TCCAGGAAGAGGAAAGGAAAAACACAAATATGACTCATCTTCACTTAGCC25030.6760443169602585No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT23790.6425527087688593No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG23350.6306685897332015No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC22550.6090611005774601No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA22530.6085209133485666No Hit
GTTCAAATGAGTAGACACAGCTTCTTCTCTGTTTCAGGGCACGGTAGAAG20960.5661162158804242No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC19820.5353255438334927No Hit
CCATGGGACTGACTTTCTGCTCCGTCCAAGCAATGGATGATTTGATGTAG19290.521010582267814No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG19240.5196601141955801No Hit
AAGGGTGCAGTTATGCCTCAACACTGACACTCAAAATGCCGTTTTCTTGT19050.5145283355210916No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA18640.5034544973287741No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACATGTCGTC16250.4389021234759967No Hit
AACAGAGAATCTCCATTTTAGCACCTTTGTCATATTTTTCCCCTCTGTGT15650.4226965066091907No Hit
GTTTCCTCTGGTCCTGGTATGAGGTTTTGTTAGGAAGGAAAAGACAAGGC15530.41945538323582954No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG14730.39784789408008814No Hit
GTTCAAATGAGTAGACACAGCTTACCGTATCAGGGCACGGTAGAAGTTGT14040.37921143468326124No Hit
ATCTCCGCAAGAAAGGGGAGATAGAACTGCCCAACAACACCAGTGTAGAA13570.3665170348042632No Hit
CCTGGTCCTCTGACTGCTCTAGACTTGGTCTCAGCCAGAATGCCAGAGGC13490.364356285888689No Hit
AACAGAGAATCTCCATTTTAGCACCGGTAGCAGAGACTTTGTCATATTTT13150.35517310299749894No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC13080.3532824476963715No Hit
AGGGATGCAAAGGCCTCATCTCGCTGGCAGGGATGTAGAACCATGTCGTC13060.35274226046747803No Hit
CTGATGGGACCCACTCCATGTCGTCAGTGTTCCTATCAGAGCAAGCATTA12740.3440992648051815No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC12610.3405880478173735Illumina Single End PCR Primer 1 (100% over 21bp)
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT12300.3322151457695237No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC12250.3308646776972899No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA12000.32411233733612066No Hit
GTTTCCTCTGGTCCTGGTATGAGGAAAGGCAATCTGAGTTATCTGTTGCT11820.3192506522760789No Hit
CAGCCAGACTGCCTTCCGTATACGGTAGCAGAGACCTGTGGGAAGCGAAA11790.3184403714327386No Hit
TCTAGATATGGTTAAGAAAACTGTTCCACACATAGCGCCTCTGACTGTGT11580.3127684055293565No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG11480.31006746938488877No Hit
GGCCTCAGTACAAACTCATTAGCGTAGCAGAGACTTGGACCTAATTACGA11300.305205784324847No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT10940.29548241420476334No Hit
AACAGAGAATCTCCATTTTAGCACACTTTGTCATATTTTTCCCCTCTGTG10870.29359175890363604No Hit
CCACACAGCAAAGCAGAAACGTTAACTGGCAATTGTGATGTAGAACCATG10640.28737960577136035No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG10360.2798169845668509No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA10110.27306464420568166No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCACTA9670.26118052517002394No Hit
AAGGGTGCAGTTATGCCTCACCCTTAGAACACTCAAAATGCCGTTTTCTT9090.24551509553211145No Hit
ACAAGTCAACAACCCCCACAGACTTCCATTTTCTACTTTTTCTGAGGTGT9070.2449749083032179No Hit
AGGGATGCAAAGGCCTCAGTGTATCTCGCTGGCAGGGATGTAGAACCATG8970.2422739721587502No Hit
CTTGGGCCTGTGTTATCTCCTGGGCCTGTGTTATCTCTTGGGCCTGTGTT8950.24173378492985667No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA8440.22795901059307158No Hit
CCATGGGACTGACTTTCTGCTACCATCCAAGCAATGGATGATTTGATGTA7870.21256367456960581No Hit
TCCCTGCTTCTGTCTCCTACAGCCTTCTTTAAGCAATGCCATTTTGGGTT7260.19608796408835302No Hit
GGTCTTTGCCTGCTGAGAGTGTCGTCAGTGTACTCCAAAGCCTCTTGCTC6960.18798515565495003No Hit
ACAAGTCAACAACCCCCACAACACTGACTTCCATTTTCTACTTTTTCTGA6930.1871748748116097No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT6710.18123281529378082No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA6680.1804225344504405No Hit
CCTGGTCCTCTGACTGCTCTGCTACCGACCAGAATGCCAGAGGCTGTGTA6600.17826178553486638No Hit
GGCCTTTTCCTTCAAACAGGATTTACGGTAGCATCTTGTTCTGTTTGTGG6460.17448047493261165No Hit
ATGAGGTACTCGTCGGCATCACACTGACGACTTGGCTATAGGAGCAGGGG6410.1731300068603778No Hit
CTTGGGCCTGTGTTATCTCCCATGGTTCTACATGTCCTGCCTGCTTGCCT6280.16961878987256984No Hit
TCCGTCTACTCCCACGTTCTGTAGCAGAGAGATGGCAGAAGCTGCTGTAG6180.16691785372810217No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTGGTTGGAAAGCCATAGGATTC6000.16205616866806033No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA5970.16124588782472005No Hit
TCTCCCAATGAAAGTAAAGTACAAACCCTTTAGCTGGCAGACCACATGTA5930.16016551336693297No Hit
CTGATGGGACCCACTCCATGTCGTCCAGTGTTCCTATCAGAGCAAGCATT5890.1590851389091459No Hit
CCACACAGCAAAGCAGAGACGTTAACTGGCAATTGTGATGTAGAACCATG5880.15881504529469914No Hit
GCTGACCTAAAGCCACCTCCTGAGTAGCTGGGATTACAGATGCCCTGAAA5860.1582748580658056No Hit
TCTGGATCAGAGTCAGTGGTGAAAACCCACAAATATGACTCATCTTGTAG5800.156654296379125No Hit
TTCATGGATTTGACGGCTCCTCTACTGTAGAACCATGTCGTCAGTGTAGA5780.15611410915023147No Hit
GGACCTAATTACGAGTGACACTGTAGAACCATGTCGTCAGTGTAGATCTC5720.15449354746355087No Hit
CTTGGGCCTGTGTTATCTCCCTGCTTGCCTCTGACCCCTGTAGAACCATG5580.15071223686129612No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA5550.14990195601795583No Hit
CTTGGGCCTGTGTTATCTCCTGGGCCTGCTTGCCTCTGACCCCTGTAGAA5300.14314961565678666No Hit
CCAACTGGCCAAGACCTTACGGGTAGCGATGGTGAGCAGCTTGTAGAACC5220.1409888667412125No Hit
CTTGGGCCTGTGTTATCTCCCACTGACGACACTGCTTGCCTCTGACCCCT4920.13288605830780947No Hit
AGGGATGCAAAGGCCTCATCCATCTCGCTGGCAGGGATGTAGAACCATGT4830.1304552157777886No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC4660.12586362433219353No Hit
CTTGGGCCTGTGTTATCTCCTGCTTGCCTCTGACCCCTGTAGAACCATGT4610.12451315625995969No Hit
CCTCCTGGCCCCTGTCGTCAGTGACTTCAATGCCTGGCCGTATTGTAGAA4410.11911128397102437No Hit
GGCCTCAGTACAAACTCATTAGCCTTGGTCTGGACCTAATTACGAGTGAC4300.11614025421210993No Hit
CCCCCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG4100.11073838192317458No Hit
GCTGACCTAAAGCCACCTCCCAACTTCTACCATGCCCTGAAACAGAGAAG4100.11073838192317458No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTACACTGGGTCGTAGCCATTTA4020.10857763300760044No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC3900.10533650963423923No Hit
CCTGGTCCTCTGACTGCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGA3840.10371594794755862No Hit
CCTGGTCCTCTGACTGCTCTTACGGTACCAGAATGCCAGAGGCTGTGTAG3770.10182529264643125No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)