FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008104510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008104510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103649
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA1516814.634005152003397No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACGTTCTTCATACCAGGACCA75797.312178602784397No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA68616.619456048780018No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTAGCTACCTGTTAAAGAATCAT37263.5948248415324797No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT35713.4452816717961583No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC35173.3931827610493106No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA29642.8596513232158536No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA28572.7564182963656187No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC19941.923800519059518No Hit
CAGCCTCTGGCATTCTGGCCGTAAGAGCAGTCAGAGGACCAGGAGACCAA18991.832145027930805No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC18521.7867996796881784No Hit
GGAATCCTATGGCTTTCCAACCTTCTACAGCAACAGAGGAGGGGGAGACC17741.7115456974982872No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGCACGTAGCTAT17411.6797074742641027No Hit
GGGGTCAGAGGCAAGCATGGAGATAACACAGGCCCAAGAGACCAAGTCTC15621.507009233084738No Hit
TCAAATCATCCATTGCTTGGTAGCAGAAAGTCAGTCCCATGGAGACCAAG14361.3854451080087604No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG13731.3246630454707715No Hit
TCCCTGCCAGCGAGATGGATGAGGCCTTTGCATCCCTAGACCAAGTCTCT13401.2928248222365868No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA11841.1423168578568053No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT11481.1075842506922402No Hit
TCCACAAACAGAACAAGATGCTACCGTAAATCCTGTTTGAAGGAAAAGGC11431.1027602774749394No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC11091.0699572595972948No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC10200.9840905363293424No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA7590.7322791343862459No Hit
CGAGCAAGAGGCTTTGGAGTACACTGACGACATGGTTCTACTCTCAGCAG6730.6493067950486738No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA5720.5518625360591998No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAG5260.5074819824600334No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTATACGGAAGGCAGTCTGGCTG5210.5026580092427327No Hit
GGAATCCTATGGCTTTCCAACCCATGGTTCTACAGCAACAGAGGAGGGGG5120.49397485745159136No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTAGCA4680.4515238931393453No Hit
TCCCTGCCAGCGAGATACACTGAGGCCTTTGCATCCCTAGACCAAGTCTC4410.42547443776592153No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT4220.4071433395401789No Hit
GGAATCCTATGGCTTTCCAACCACACTGACGACATGGTACTACAGCAACA3820.3685515538017733No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCGGAAAGACATTATGACA3440.331889357350288No Hit
GGAATCCTATGGCTTTCCAACCCCGCATGGTTCTACAGCAACAGAGGAGG3370.325135794846067No Hit
TGAGCAAGAGGCTTTGGAGTACACTGACGACATGGTTCTACTCTCAGCAG3020.29136798232496214No Hit
TCATAATGCTTGCTCTGATAGGAGTGGGTCCCATCAGAGACCAAGTCTCT2600.25084660729963626No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTAGCACGTAGCTATCT2340.22576194656967266No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC2130.20550125905700972No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT2120.20453646441354958No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTAGCA1950.1881349554747272No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTGTGACA1770.1707686518924447No Hit
CAGCCTCTGGCATTCTGGCCATAAGAGCAGTCAGAGGACCAGGAGACCAA1670.1611207054578433No Hit
TGAGCAAGAGGCTTTGGAGTACACTGACGACATGGTTCCACTCTCAGCAG1570.1514727590232419No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA1560.15050796437978176No Hit
GGATGCAGAGCTTCTTCCCAGTAGCAGAGACTGGGTGCGGAAGAGAAAGA1380.13314166079749926No Hit
GGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA1330.12831768758019857No Hit
GCGTCTTCACCTGGACTATGTCCGGAGACCAAGTCTCTGCTACCGTAGCA1280.12349371436289784No Hit
AGGACAAAATGTTTCACTTTTGGGTAAGTGCAGATAATGACAAGGAATAT1230.11866974114559715No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTAGAC1110.10709220542407549No Hit
GCGTGGACAACCCCCACCACTGACTCTGATCCAGAAGACCAAGTCTCTGC1080.10419782149369507No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGCACGTAGCTAT1070.10323302685023492No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)