FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008104511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008104511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103649
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA1545714.912830803963375No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC74327.170353790195756No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA68846.6416463255796No Hit
TCCAGATGATTCTTTAACAGGTAGCTAGCAGAGACTGGGTCGTAGCCATT36503.5215004486295087No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT36093.481943868247644No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA35213.3970419396231515No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA29542.850003376781252No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT28802.7786085731652017No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA19951.9247653137029783No Hit
CCTGGTCCTCTGACTGCTCTTACGGCCAGAATGCCAGAGGCTGTGTAGAA19151.8475817422261671No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA18681.8022363939835406No Hit
CCCCCTCCTCTGTTGCTGTAGAAGGTTGGAAAGCCATAGGATTCCTGTAG17961.7327711796544105No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG17801.7173344653590483No Hit
CTTGGGCCTGTGTTATCTCCATGCTTGCCTCTGACCCCTGTAGAACCATG15561.5012204652239771No Hit
CCATGGGACTGACTTTCTGCTACCAAGCAATGGATGATTTGATGTAGAAC14481.397022643730282No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG13721.3236982508273114No Hit
AGGGATGCAAAGGCCTCATCCATCTCGCTGGCAGGGATGTAGAACCATGT13451.2976487954538878No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT13231.2764233132977647No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG11841.1423168578568053No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC11611.1201265810572218No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC11561.1153026078399213No Hit
GGCCTTTTCCTTCAAACAGGATTTACGGTAGCATCTTGTTCTGTTTGTGG11341.0940771256837982No Hit
GGTCTTTGCCTGCTGAGAGTAGAACCATGTCGTCAGTGTACTCCAAAGCC10150.9792665631120415No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA5600.5402850003376781No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA5440.5248482860423158No Hit
CAGCCAGACTGCCTTCCGTATACGGTAGCAGAGACCTGTGGGAAGCGAAA5170.49879883066889213No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGGGTTGGAAAGCCATAGGATTCC5030.48529170566045016No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA4660.44959430385242505No Hit
AGGGATGCAAAGGCCTCAGTGTATCTCGCTGGCAGGGATGTAGAACCATG4630.4466999199220446No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG4560.4399463574178236No Hit
CCCCCTCCTCTGTTGCTGTAGTACCATGTCGTCAGTGTGGTTGGAAAGCC3870.373375527019074No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT3800.366621964514853No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGCGGGGTTGGAAAGCCATAGGAT3470.3347837412806684No Hit
GTTTCCTCTGGTCCTGGTATGAAGAACGTATTTACCCAAAAGTGAAACAT3190.3077694912637845No Hit
CTCGATAATCTGGATGACTCATTATTGTTACGACAGTAAGATACAGTCTA2680.25856496444731736No Hit
CTGATGGGACCCACTCCTATCAGAGCAAGCATTATGATGTAGAACCATGT2590.24988181265617615No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC2350.22672674121313277No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT2190.21129002691777055No Hit
CCTGGTCCTCTGACTGCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGA2000.1929589286920279No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC1650.15919111617092302No Hit
CCTGGTCCTCTGACTGCTCTTATGGCCAGAATGCCAGAGGCTGTGTAGAA1650.15919111617092302No Hit
TCTAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG1600.1543671429536223No Hit
GGTCTTTGCCTGCTGAGAGTGGAACCATGTCGTCAGTGTACTCCAAAGCC1600.1543671429536223No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC1380.13314166079749926No Hit
TTCTCTTCCGCACCCAGTCTCTGCTACTGGGAAGAAGCTCTGCATCCTGT1350.13024727686711884No Hit
CCGGACATAGTCCAGGTGAAGACGCTGTAGAACCATGTCGTCAGTGTAGA1330.12831768758019857No Hit
CTCGATAATCTGGATGACTCATTATGTTATGACAGTAAGATACAGTCTAT1290.12445850900635799No Hit
ACTAGATATTCCTTGTCATTATCTGCACTTACCCAAAAGTGAAACATTTT1190.1148105625717566No Hit
CGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTATC1170.11288097328483633No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATATATGAACTTGTCCTCC1140.1099865893544559No Hit
CCCCCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG1140.1099865893544559No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGTA1110.10709220542407549No Hit
TCTGGATCAGAGTCAGTGGTGGGGGTTGTCCACGCTGTAGAACCATGTCG1090.10516261613715522No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)