FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008104528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008104528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136214
Sequences flagged as poor quality0
Sequence length150
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA1637612.022259092310629No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA1591711.685289324151702No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG128499.432951091664586No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA61244.4958667978328215No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCGGCTACCTGTTAAAGAAT39722.9159998238066573No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA35562.610598029571116No Hit
GGGGTCAGAGGCAAGCAGTCACAGCACATGTAGAACCCATGTCGGGAGAT35222.5856373060037883No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA29932.1972778128533044No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTATGTGGGGGTTG28252.0739424728735667No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT26521.9469364382515748No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC25941.9043563804014272No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG20021.4697461347585417No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC18471.3559546008486645No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACATGCATCATAT17971.3192476544261236No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTCCATACCAGGACCA16991.2473020394379433No Hit
TGACAGTTTGACAGTTAAAGGCAAAGATATATTCCTCCAATTCAGGACCC16971.2458337615810415No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG15961.1716857298075087No Hit
GGAAACCGTAGCTGCCCTGTCCACTGACGACATGGTTCATCTGGACCTGG13160.9661268298412792No Hit
GGAAACCGTAGCTGCCCTGGCGTCTTCATCTGGACCTGGGTCTTAGACCA11820.8677522134288693No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT11230.8244380166502708No Hit
TGACAGTTTGACAGTTAAAGGCATAGATATATTCCTCCAATTCAGGACCC10760.7899334870130824No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG10400.7635044855888529No Hit
TCCACAAACAGAACAAGATGCTACCGTAAATCCTGTTTGAAGGAAAAGGC9350.6864198981015168No Hit
ATACGGCCAGGCATTGAAGTCAACTGACGACAGGGGCCAGGAGGAGACCA8610.6320936173961561No Hit
GGAATCCTATGGCTTTCCAACCCATGGTTCTACAGCAACAGAGGAGGGGG8520.6254863670400986No Hit
CAGCCTCTGGCATTCTGAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCT7890.579235614547697No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC7440.5461993627674101No Hit
GGAATCCTATGGCTTTCCAACCTGGTTCTACAGCAACAGAGGAGGGGGAG7020.5153655277724757No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC6940.5094924163448691No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC6020.44195163492739364No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA5470.40157399386259857No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAC4900.3597280749409018No Hit
GGAATCCTATGGCTTTCCAACCCACTGACGACATGGTTCTACAGCAACAG4720.3465135742287871No Hit
TGTGGTCTGCCAGCTAAAGTTTGTACTTTACTTTCATTGGGAGAAGACCA4250.3120090445915985No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACACTCTTCATACCAGGACCA4220.30980662780624607No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTACAT3830.2811752095966641No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT3620.2657582920991968No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA3570.26208759745694277No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA3280.24079756853186896No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACGTTCTTCATACCAGGACCA3170.23272204031890995No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTACATGCATCATATCT2750.2018882053239755No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTACAT2570.18867370461186075No Hit
AGGACAAAATGTTTCACTTTGGGTAAATACATTCTTCATACCAGGACCAG1890.138752257477205No Hit
TGTGGTCTGCCAGCTAAAGGTTTGTACTTTACTTTCATTGGGAGAAGACC1860.13654984069185253No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT1780.13067672926424598No Hit
GGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA1750.12847431247889352No Hit
AGGACAAAATGTTTCACTTTTGGTAAATACATTCTTCATACCAGGACCAG1690.12406947890818859No Hit
GTAGAGGAGCCGTCAAATCCATGAAAGACCAAGTCTCTGCTACCGTACAT1680.12333533997973777No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG1610.11819636748058203No Hit
GGTCTTCTCTGTTTCAGGGCATCACTGACGACATGGTTCTACAGCAACAG1480.10865256141072137No Hit
GCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTACATGCATCATA1400.1027794499831148No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)