FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008104529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008104529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136214
Sequences flagged as poor quality0
Sequence length150
%GC40

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC1708212.540561175796908No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA1611111.82771227627116No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA127379.350727531678094No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA61194.492196103190568No Hit
TCCAGATGATTCTTTAACAGGTAGCCGGTAGCAGAGACTGGGTCGTAGCC38412.8198276241795996No Hit
CTTGGGCCTGTGTTATCTCCCGACATGGGTTCTACATGTGCTGTGACTGC36882.707504368126624No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA35822.629685641710837No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC33542.4623019660240506No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT30232.219301980706829No Hit
ACAAGTCAACAACCCCCACATACGGTAGCAGAGACTTCCATTTTCTACTT27352.007869969312993No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT26891.9740995786042552No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA26071.9139001864712877No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG19911.4616706065455827No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA18421.3522839062064105No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG18301.3434742390650007No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT15831.162141923737648No Hit
AAGACCCAGGTCCAGATGAACCATGTCGTCAGTGGACAGGGCAGCTACGG13200.9690633855550825No Hit
AAGACCCAGGTCCAGATGAAGACGCCAGGGCAGCTACGGTTTCCTGTAGA11920.8750936027133775No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG11620.8530694348598529No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT10990.8068186823674512No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC10590.7774531252294185No Hit
GGCCTTTTCCTTCAAACAGGATTTACGGTAGCATCTTGTTCTGTTTGTGG9300.6827492034592627No Hit
CCTCCTGGCCCCTGTCGTCAGTTGACTTCAATGCCTGGCCGTATTGTAGA8670.636498450966861No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGGGTTGGAAAGCCATAGGATTCC8500.624018089183197No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC8100.5946525320451642No Hit
CCTGGTCCTCTGACTGCTCTCAGAATGCCAGAGGCTGTGTAGAACCATGT7900.5799697534761479No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA7380.5417945291967051No Hit
CCCCCTCCTCTGTTGCTGTAGAACCAGGTTGGAAAGCCATAGGATTCCTG6970.5116948331302216No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA5120.3758791313668199No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGGGTTGGAAAGCCA4980.36560118636850836No Hit
TCTCCCAATGAAAGTAAAGTACAAACTTTAGCTGGCAGACCACATGTAGA4270.3134773224485002No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA3870.28411176531046733No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA3560.26135345852849196No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT3420.2510755135301804No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG3300.24226584638877063No Hit
GTTTCCTCTGGTCCTGGTATGAAGAACGTATTTACCCAAAAGTGAAACAT2910.21363442817918862No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC2810.20629303889468042No Hit
CCTGGTCCTCTGACTGCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGA2590.19014198246876238No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC2430.17839575961354925No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTAGCTGGCAGACCACATGTAG1880.13801811854875418No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT1820.13361328497804922No Hit
CCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA1790.1314108681926968No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGGCAGTAAGATACAGTCTA1730.12700603462199186No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC1670.12260120105128695No Hit
TTCATGGATTTGACGGCTCCTCTACTGTAGAACCATGTCGTCAGTGTAGA1630.11966464533748365No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGA1610.11819636748058203No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG1550.11379153390987709No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC1460.10718428355381972Illumina Single End PCR Primer 1 (100% over 21bp)
CGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTATC1430.10498186676846727No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)