FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008104532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008104532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432149
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA213264.934872000166609No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC181554.201097306715971No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT169883.9310515586059442No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA169263.9167046551073823No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG151763.5117517337770074No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA120132.779828253681022No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA119502.7652499485131288No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG116622.6986062677456157No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG96202.2260840589704016No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC92642.1437050646883367No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG88432.046284961899715No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT83271.9268817005245877No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG79011.828304589389308No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG77231.7871150922482755No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC73751.7065873113208638No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA70081.621662898676151No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT62301.4416323999361331No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT57661.3342620253662512No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT52941.2250404374417156No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT51301.1870905636713263No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC51011.1803799152607086No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA50691.172975061842096No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT45401.0505635787656573No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGAGGTGGGAGGCTG44141.02140696842987No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT41570.961936739411638No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGTGATACTGAAT40120.92838349735855No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT36540.8455416997378219No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC33180.76779073884239No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC33100.7659395254877368No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT32810.7592288770771193No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT29240.6766184811257229No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG28290.6546353225392167No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA27900.6456106574352827No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC27040.6257101138727614No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC26770.6194622688008071No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA25720.5951650935209847No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA24840.5748017466198001No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC24150.5588350314359167No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA24020.5558268097346054No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG23680.5479591529773297No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC22050.5102406808762718No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA20670.4783072505085052No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGAAGGGCCAGGAA19260.44567961513274357No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG18600.4304071049568552No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC17720.4100437580556706No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC17700.4095809547170073No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG15270.3533503490694182No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTATGTGGGGGTTG13310.30799562188041624No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG13270.30707001520308963No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG13090.3029047851551201No Hit
TGACAGTTTGACAGTTAAAGGCATGTCGCAGTGCAGCCGTTACCTGTGTG11780.27259116647267495No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG11520.26657472307005226No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA11360.26287229636074594No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG10280.23788091607292852No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG9450.21867457751840225No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT9240.21381514246243774No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC9190.2126581341157795No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA9180.21242673244644789No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC9130.21126972409978967No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTACGTGATCAAGAAATCGATAGC8490.19646001726256454No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG8480.19622861559323287No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTAGGAGGCTG7950.18396432711865585No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGACGCGGACGCGGGTGCCGGGC7740.17910489206269134No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTAGTGATACTGAATCTCG7520.1740140553373952No Hit
GGATGCAGAGCTTCTTCCCAAGTCTCTGCTACCGCTGGGTGCGGAAGAGA7400.17123723530541549No Hit
GTAGAGGAGCCGTCAAATCCATGAAAGACCAAGTCTCTGCTACCGTAGTG7380.1707744319667522No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCTACAGGCTCCCAG6300.14578305167893482No Hit
TGTGGTCTGCCAGCTAAAGGCATGTGGTTTGTACTTTACTTTCATTGGGA5970.1381467965909906No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAG5900.1365269849056691No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA5770.13351876320435774No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC5640.1305105415030464No Hit
ACTTCTACCGTGCCCTGATGGATGAAGGAGACATGGACGACGTGGTGGAT5380.1244940981004237No Hit
CCCCTGCTCCTATAGCCAAGAAGTGAAATAGCATCTCTACGGGCCATTCT5070.11732064635114278No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGGCGCGGGTGCCGGGC5060.11708924468181113No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAAACCTAAGCTGTGTCTACTCATT4890.11315541630317322No Hit
CAGAGGGGAAAAATATGACAAAGTGCTAAAATGGAGATTCTCTGTTAGAC4850.11222980962584664No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT4820.1115356046178517No Hit
TGTGGTCTGCCAGCTAAAGGGTTTGTACTTTACTTTCATTGGGAGAAGAC4610.10667616956188722No Hit
TCAAATCATCCATTGCTTGGCGGGTAGCAGAAAGTCAGTCCCATGGAGAC4500.10413075119923915No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACCGTAGTGCTAAAATGGA4490.10389934952990752No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACGTAGTGCTAAAATGGAG4420.10227953784458602No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)