FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008104538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008104538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477791
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA217164.545083519781662No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC179723.761477298651503No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA176493.6938745183563526No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT172743.6153883183232836No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA127992.6787863312619953No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG127402.666437835790126No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG124852.613067219767639No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT119182.494396085317639No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA110962.322354334845152No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC107602.252030699615522No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG100212.0973605614170214No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC97982.050687434464023No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG93921.9657130418948872No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT92951.945411278153No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA91751.9202956941424179No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA86981.8204612477003543No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT77071.6130483830796312No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT76581.6027928529419768No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT74271.5544453537216063No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG73211.5322599211789256No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG72471.5167719777057334No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG67131.4050076288586433No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC61751.292406093877867No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT58361.221454569047973No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG53751.1249688671406535No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG52371.0960859455284844No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC52281.0942022767276907No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG51171.0709703615179023No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA47060.9849494862816588No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT41350.8654411656979725No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT40030.8378140232863324No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT38020.7957454200686075No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT37980.7949082339349214No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTGTGTGATCAAGAAATCGATAGC37360.7819318488627873No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA36770.7695833533909178No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC36480.7635137539216937No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC31780.6651443832135808No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATAGGGGTGGGAGGCTG28200.5902162242486778No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG28170.5895883346484132No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC27950.5849838109131399No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACTATACAGTGAT26480.5542172205001769No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGGGATGGGAGTGGGAGGCTG26370.5519149586325401No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG25940.5429152076954149No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA25660.5370549047596125No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA24530.513404396482981No Hit
GAGAAAGCCCCCCTACTGCTCACCTGGAGGGCCACTGACAACCACCCTTA21480.44956895378941836No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC19330.4045701991037922No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC16930.3543390310826282No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG16580.3470136524128751No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG15490.3242003302699297No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA14160.296363891324868No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC13520.2829689131858909No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA12900.26999252811375685No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCAGTGTGTGGGGGTTGTTGACTTG12420.259946294509524No Hit
TGTGCTGTGACTGCTTGTAGACAGAGTTGAAAGTCAGGGCAAGACCAAGT11670.2442490545029103No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG9580.20050607901781325No Hit
GCAGCTTCTGCCATCTCTCTTCTCCTTTTTCTTCAGCCACAGGCTCCCAG8870.18564602514488554No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACCCGGATAAGATGCTGAGG8600.17999501874250456No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGTCCCTCAGGGCAACTG8290.17350682620643754No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG8240.17246034353932996No Hit
CAGAGGGGAAAAATATGACAAAGTCCCGTGCTAAAATGGAGATTCTCTGT8090.1693208955380072No Hit
TCCACAAACAGAACAAGATGCTACCGTAAATCCTGTTTGAAGGAAAAGGC8070.16890230247116417No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATAACA7750.16220481340167564No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA7640.15990255153403896No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT7420.15529802779876556No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTAG7350.15383295206481495No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGAGTCTTCAGTGAAC7290.15257717286428585No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCTAGCCCCTCAGGGCAACTG6460.13520556059029995No Hit
ACTTCTACCGTGCCCTGATGGATGAGGAAGACATGGACGACGTGGTGGAT6330.1324847056558202No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC6280.13143822298871266No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA5830.12201987898474438No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTG5400.11302012804761914No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAC5360.11218294191393309No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATGTTT5320.11134575578024702No Hit
ACTTCTACCGTGCCCTGATACAGATGTAGAAGCTGTGTCTACTCATTTGA5180.10841560431234577No Hit
TGTGCTGTGACTGCTTGTAGAAACAGAGTTGAAAGTCAGGGCAAGACCAA4980.10422967364391543No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)