FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008104556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008104556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408828
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA240465.8816910779105145No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC232605.6894341874822665No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT210325.144461729627129No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGACCAAGTCTCAAGCTGTGTCTA180034.4035633567172505No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACGTCTCTGCTACCGTAGGGACG117682.8784721203048718No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA115182.8173217098633163No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA93442.285557740663555No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC91382.235169802459714No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG88952.1757316035105227No Hit
TGTGGTCTGCCAGCTAAAGGGCCCGTTTAAACCGGTTTGTACTTTACTTT73901.8076061326523625No Hit
GCAGCTTCTGCCATCTCTCTGCTACCGTAAGAACGTGGGAGTAGACGGAA68461.6745428395315387No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG61701.5091921296975745No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA56941.3927617482168542No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA56651.3856683006056336No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG53381.30568356374808No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG52641.2875830422573797No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATACTGCAGCGAT51201.252360405843044No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC46041.1261459586916747No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTATGTGGGGGTTG45681.1173402995880908No Hit
AGAAAACGGCATTTTGAGTGTACACTGACGACATGGTTCTACAAGAAAAC44741.0943477452620662No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG43731.0696429794436781No Hit
CAGAGGGGAAAAATATGACAAACTTGTGCTAAAATGGAGATTCTCTGTTA43711.0691537761601457No Hit
AGGACAAAATGTTTCACTTTTGGGTAAGTGCAGATAATGACAAGGAATAT39910.9762051522889823No Hit
TCTGTATCAGGCAAAGTCATAGAAGCAGACCAAGTCTCTGCTAGCGTAGG37910.9272848239357383No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT36900.9025800581173501No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACCGGTGCTAAAATGGAGA34930.8543935346894049No Hit
GCAACAGATAACTCAGATTGCCTTCACTGACGACATGGTTCATACCAGGA32600.7974013521578757No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTACCGTAAACAATAATG31820.7783224241001105No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA31540.7714735781306564No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG31270.7648693338029685No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT29090.7115461758979327No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC28170.6890428248554404No Hit
GGATGCAGAGCTTCTTCCCATGTCCTGGGTGCGGAAGAGAAAGACCAAGT26060.6374318784427682No Hit
GGGGTCAGAGGCAAGCAGGAGATAACACAGGCCCAAGAGACCAAGTCTCT24830.6073458765055231No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT24290.5941373878501472No Hit
GGAATCCTATGGCTTTCCAACCTGGTTCTACAGCAACAGAGGAGGGGGAG23410.57261244337472No Hit
TCATAATGCTTGCTCTGATAGGAACACTGACGACATGGAGTGGGTCCCAT22740.5562241333763832No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCCTCCCATGTGGGGGTTGTTGACT19540.47795160801119296No Hit
TCCACAAACAGAACAAGATGCTACCGTAAATCCTGTTTGAAGGAAAAGGC17460.42707446652381936No Hit
AGAAAACGGCATTTTGAGGCATAACTGCACCCTTAGACCAAGTCTCTGCT16570.40530492040662575No Hit
TCCCTGCCAGCGAGATGATGAGGCCTTTGCATCCCTAGACCAAGTCTCTG15880.3884274071247566No Hit
GGAATCCTATGGCTTTCCAACCCACTGACGACATGGTTCTACAGCAACAG15790.3862259923488607No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCGTAGCTACCTGTTAAAGA14870.36372264130636844No Hit
TCTTAAATGGCTACGACCCAGTGTCTGCTACCTGTTAAAGAATCATCTGG14000.34244229847270735No Hit
TCCCTGCCAGCGAGATACTTGAGGCCTTTGCATCCCTAGACCAAGTCTCT13610.3329028344438248No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAT13150.3216511589225787No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAATTCATCTGGACCTGGGTC12910.3157807195201894No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC12780.3126008981772286No Hit
TCAAATCATCCATTGCTTGGTACGGGCAGAAAGTCAGTCCCATGGAGACC12150.2971909947459567No Hit
GGTCTTCTCTGTTTCAGGGCATGGTAGAAGTTGTAGAACGGAGGTGGCTT12040.29450037668652834No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC10610.2595223419139589No Hit
GGGGTCAGAGGCAAGCAGGGCTCCAAGGGGAGATAACACAGGCCCAAGAG10380.25389650415333587No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGCTACCGTATACTGCA10250.25071668281037507No Hit
GGTCTTCTCTGTTTCAGGGCATGGTAGAAGTTGGGAGGTGGCTTTAGGTC9320.2279687301261166No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTATACTGCAGCGATCTCG8820.21573864803780562No Hit
TCAAATCATCCATTGCTTGGTACGTAGCAGAAAGTCAGTCCCATGGAGAC8730.21353723326190968No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC8110.19837193147240403No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTATAC8040.1966597199800405No Hit
TCAAATCATCCATTGCTTGGTAGGTAGCAGAAAGTCAGTCCCATGGAGAC7920.19372450027884586No Hit
TCAAATCATCCATTGCTTGGTACGGGTAGCAGAAAGTCAGTCCCATGGAG7750.18956627236882015No Hit
GGGGTCAGAGGCAAGCAGTGGGGAGATAACACAGGCCCAAGAGACCAAGT7530.1841850362499633No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCAGTGTTGTGGGGGTTGTTGACTT7210.1763577837134443No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA6960.1702427426692888No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTATACTGCAGCGATCT6910.1690197344604577No Hit
ATACGGCCAGGCATTGAAGTCACTGACGACAGGGGCCAGGAGGAGACCAA5940.1452933752091344No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA5910.14455957028383573No Hit
ATACGGCCAGGCATTGAAGTACACTGACAGGGGCCAGGAGGAGACCAAGT5770.14113514729910867No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTATACGGAAGGCAGTCTGGCTG5750.14064594401557623No Hit
GGGGTCAGAGGCAAGCAGGCCTCTGCTACCGTAGGAGATAACACAGGCCC5710.13966753744851135No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTATAC5550.13575391118025185No Hit
ATACGGCCAGGCATTGAAGTCACACTGACGACAGGGGCCAGGAGGAGACC5540.1355093095384856No Hit
GGTCTTCTCTGTTTCAGGGCATGACCAAGTCTCAGCAACAGAGGAGGTGG5240.12817126028549902No Hit
GTAGAGGAGCCGTCAAATCCATGAAAGACCAAGTCTCTGCTACCGTATAC5200.12719285371843417No Hit
GGGGTCAGAGGCAAGCAGGACATGTAGAACGGAGATAACACAGGCCCAAG5080.12425763401723952No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT5000.12230082088310976No Hit
TCTGTATCAGGCAAAGTCATAGAAGCAGACCAAGTCTCTGCTACCGTATA4900.11985480446544756No Hit
GGAAACCGTAGCTGCCCTGGGTCTGCTACCGTATTCATCTGGACCTGGGT4850.11863179625661646No Hit
CTGACGACATGGAGTGGGTCCCATCAGAGACCAAGTCTCTGCTACCGTAT4820.11789799133131783No Hit
CCCCTGCTCCTATAGCCAAGTCTCTGCTACCGTGATGCCGACGAGTACCT4700.11496277163012317No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGAAT4640.11349516177952587No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT4640.11349516177952587No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA4420.10811392566066903No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)