FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008104557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008104557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408828
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA236965.796080503292338No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA220595.395667615721036No Hit
GTTCAAATGAGTAGACACAGCTTGAGACTTGGTCTTCTCTGTTTCAGGGC181624.442455017758079No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA144103.524709657851224No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA115842.833465418219887No Hit
TCATTACACCAGTTCGTCCCTACGGTAGCAGAGACGTGGTAAAAAGATAA111182.7194810531568288No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA93882.296320212901269No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA91802.2454430714138955No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG88792.1718179772422634No Hit
AAGGGTGCAGTTATGCCTCAAAATGCCGTTTTCTTGTAGAACCATGTCGT79151.9360219945796275No Hit
TCTCCCAATGAAAGTAAAGTACAAACCGGTTTAAACGGGCCCTTTAGCTG70881.7337364368389638No Hit
TCCGTCTACTCCCACGTTCTTACGGTAGCAGAGAGATGGCAGAAGCTGCT68411.6733198313227078No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT61511.5045446985040163No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT57311.4018120089622041No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT57061.3956969679180486No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG56711.387135910456231No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG52951.2951656931521325No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG49671.2149363546528125No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA46301.1325056013775965No Hit
AACAGAGAATCTCCATTTTAGCACAAGTTTGTCATATTTTTCCCCTCTGT44071.0779594352637294No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC42991.0515424579529777No Hit
ACAAGTCAACAACCCCCACATACGGTAGCAGAGACTTCCATTTTCTACTT41081.00482354437563No Hit
ACTAGATATTCCTTGTCATTATCTGCACTTACCCAAAAGTGAAACATTTT37690.9219035878168814No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT37310.912608725429765No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA36680.8971988219984933No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT35570.8700480397624428No Hit
AACAGAGAATCTCCATTTTAGCACCGGTAGCAGAGACTTTGTCATATTTT35010.8563503478235346No Hit
GTTTCCTCTGGTCCTGGTATGAACCATGTCGTCAGTGAAGGCAATCTGAG33250.8133004588726799No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG31290.7653585370865009No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG30520.746524210670502No Hit
CTCGATAATCTGGATGACTCATTATTGTTTACGGTAGCACAACTAAAAGA29990.7335603236568924No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA27120.6633596524699874No Hit
TTCTCTTCCGCACCCAGGACATGGGAAGAAGCTCTGCATCCTGTAGAACC26250.6420793096363263No Hit
CTTGGGCCTGTGTTATCTCCTGCTTGCCTCTGACCCCTGTAGAACCATGT25160.6154177306838083No Hit
CCCCCTCCTCTGTTGCTGTAGAACCAGGTTGGAAAGCCATAGGATTCCTG23510.5750584597923821No Hit
CTGATGGGACCCACTCCATGTCGTCAGTGTTCCTATCAGAGCAAGCATTA23130.5657635974052658No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC23110.5652743941217333No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGGGTTGGAAAGCCA18800.45985108652049267No Hit
ACAAGTCAACAACCCCCACATGGGAGGACTTCCATTTTCTACTTTTTCTG18160.44419658144745466No Hit
AGGGATGCAAAGGCCTCATCATCTCGCTGGCAGGGATGTAGAACCATGTC17560.4295204829414815No Hit
GGCCTTTTCCTTCAAACAGGATTTACGGTAGCATCTTGTTCTGTTTGTGG17340.4241392468226247No Hit
AGGGATGCAAAGGCCTCAAGTATCTCGCTGGCAGGGATGTAGAACCATGT14930.36519025115696574No Hit
TCCAGATGATTCTTTAACAGGTAGCTACGGTAGCAGAGACTGGGTCGTAG14230.3480681362333304No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC13980.3419530951891749No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA13360.3267877933996693No Hit
TCCAGATGATTCTTTAACAGGTAGCAGACACTGGGTCGTAGCCATTTAAG13290.3250755819073058No Hit
AAGACCCAGGTCCAGATGAATTGGTCTAAGACCCAGGGCAGCTACGGTTT12480.305262848924242No Hit
CCATGGGACTGACTTTCTGCCCGTACCAAGCAATGGATGATTTGATGTAG12270.3001262144471514No Hit
GCTGACCTAAAGCCACCTCCGTTCTACAACTTCTACCATGCCCTGAAACA11960.2925435635523986No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC11730.2869177257917755No Hit
GGACCTAATTACGAGTGACACTGTAGAACCATGTCGTCAGTGTAGATCTC11300.2763998551958281No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA11050.27028481415167255No Hit
CTTGGGCCTGTGTTATCTCCCCTTGGAGCCCTGCTTGCCTCTGACCCCTG10410.25463030907863454No Hit
GCTGACCTAAAGCCACCTCCCAACTTCTACCATGCCCTGAAACAGAGAAG9260.22650112027551927No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC9120.22307669729079221Illumina Single End PCR Primer 1 (100% over 21bp)
CCATGGGACTGACTTTCTGCTACGTACCAAGCAATGGATGATTTGATGTA8890.21745085953016915No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA8450.2066883872924555No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGACAGAGATTTCATACC8240.2015517528153649No Hit
CCATGGGACTGACTTTCTGCTACCTACCAAGCAATGGATGATTTGATGTA8020.1961705166965081No Hit
CCATGGGACTGACTTTCTGCTACCCGTACCAAGCAATGGATGATTTGATG7830.19152308550294989No Hit
CTTGGGCCTGTGTTATCTCCCCACTGCTTGCCTCTGACCCCTGTAGAACC7480.1829620280411322No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG7340.17953760505640515No Hit
TCTAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG7190.17586858042991185No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC7000.17122114923635368No Hit
ACAAGTCAACAACCCCCACAACACTGACTTCCATTTTCTACTTTTTCTGA6720.16437230326689953No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA6120.14969620476092638No Hit
CCTCCTGGCCCCTGTCGTCAGTGACTTCAATGCCTGGCCGTATTGTAGAA6030.14749478998503038No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGAGACAGAGATTT5900.14431496864206952No Hit
CAGCCAGACTGCCTTCCGTATACGGTAGCAGAGACCTGTGGGAAGCGAAA5860.14333656207500464No Hit
CCTCCTGGCCCCTGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGAACC5850.14309196043323844No Hit
CTTGGGCCTGTGTTATCTCCTACGGTAGCAGAGGCCTGCTTGCCTCTGAC5650.13819992759791405No Hit
CCTCCTGGCCCCTGTCGTCAGTGTGACTTCAATGCCTGGCCGTATTGTAG5610.13722152103084914No Hit
CCTGGTCCTCTGACTGCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGA5570.1362431144637843No Hit
TTCATGGATTTGACGGCTCCTCTACTGTAGAACCATGTCGTCAGTGTAGA5250.12841586192726526No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT5110.12499143894253818No Hit
CTTGGGCCTGTGTTATCTCCGTTCTACATGTCCTGCTTGCCTCTGACCCC5090.12450223565900576No Hit
GCTTCTATGACTTTGCCTGATACAGATGTAGAACCATGTCGTCAGTGTAG5050.12352382909194086No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGAGACTTGGTCATGCCCTGAA4960.12132241431604489No Hit
AAGACCCAGGTCCAGATGAATACGGTAGCAGACCCAGGGCAGCTACGGTT4870.1191209995401489No Hit
CTGATGGGACCCACTCCATGTCGTCAGTGTAGAACCATGTCGTCAGTGTA4820.11789799133131783No Hit
CCATTACACCAGTTCGTCCCTACGGTAGCAGAGACGTGGTAAAAAGATAA4800.11740878804778537No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC4640.11349516177952587No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT4630.11325056013775964No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)