FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008104565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008104565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1075917
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC393373.6561370440284895No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA384153.570442701435148No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA331183.078118479399433No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA292422.7178676422066013No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT273842.5451777414057033No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA272412.5318867533462153No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA237452.2069546256820924No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC219032.035751828440298No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG218482.0306399099558794No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG187471.7424206514071252No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC184461.7144445157014898No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG182601.6971569368269113No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT179741.6705749607079359No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG173011.6080236672531432No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT166771.5500266284481052No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA166091.543706438321915No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC161991.505599409619887No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA159291.480504537060015No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC155241.4428622282202066No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT148701.3820768702418496No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA147401.3699941538241331No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT141671.316737257613738No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC140671.3074428603693407No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT135551.2598555464780277No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCC134531.2503752612887424No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT119581.1114240224850058No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG112281.043574922600907No Hit
TCTAGATATGGTTAAGAAAACTGTTCCAATACATGGAAGGATGAGAATTT109021.0132751875841723No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT107590.9999841995246845No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA106690.9916192420047272No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC94260.8760898842568711No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA91940.8545268826498698No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT85770.7971804516519397No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT85540.7950427402857283No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT82200.763999453489442No Hit
ATGAAAACACAACATGAATATAAACATCAATATTTGAAATAGAAAATCAA80050.7440164994139883No Hit
CCAACTGGCCAAGACCTGCGTGGGTTGATTCCACACCCCCGCCCGGCACC78110.7259853687598579No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG73650.6845323570498467No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA69700.6478194879344782No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA63860.5935402080271992No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT60520.5624969212309128No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT57840.5375879366159285No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGTGATGGTGAGCAGCTGGGG57720.5364726089466009No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATT55620.516954374733367No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC54320.5048716583156507No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG53280.4952054851814778No Hit
ACAAGTCAACAACCCCCACAACACTGACTTCCATTTTCTACTTTTTCTGA48550.4512429862154795No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG45540.42326685050984414No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAATTCAGACTTTTGTAATT38930.3618308847243793No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT36660.34073260297959784No Hit
TCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTCT34920.3245603517743469No Hit
TCCCTGCTTCTGTCTCCTACAGTCACCTGAAGTCCAAAAAGGGTCAGTCT34390.31963432123481644No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT33320.3096893161833115No Hit
TCTAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG29620.27530004637904226No Hit
GGCCTTTTCCTTCAAACAGGATTATTTACCCTTATAATGTCTCACCAATG29200.2713963995363955No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC28470.2646114895479856No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT26220.2436990957480921No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGGTCCATAGAAAATCTTT25180.23403292261391911No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA24400.22678329276328937No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG22510.20921688197137883No Hit
CCATGGGACTGACTTTCTGCTTACCGCCAAGCAATGGATGATTTGATGTA22400.20819449827449515No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC21860.20317552376252068No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAAATTCAGACTTTTGTAA21260.19759888541588244No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGAC19870.18467967324617046No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGAGC19520.1814266342106315No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC19370.18003247462397193No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGAC18630.17315462066311807No Hit
AACAGAGAATCTCCATTTTAGCACTAGCAGAGACTTGTCATATTTTTCCC17530.16293078369428127No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGACAGAGATTTCATACC17010.15809769712719476No Hit
CCATGGGACTGACTTTCTGCTACCAAGCAATGGATGATTTGATGTAGAAC16930.157354145347643No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGAGACAGAGATTT16250.15103395522145296No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCAATGGTACAT16040.14908213180012958No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAATCCCCCTTGCCGTC15940.14815269207568987No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA15590.14489965304015087No Hit
ACTAGATATTCCTTGTCATTATCTGCACCGTATTACCCAAAAGTGAAACA15220.14146072605972393No Hit
TCCCTGCTTCTGTCTCCTACCGTGCCATTTTGGGTTTTGGGTCTGTAGAA14510.13486170401620198No Hit
AAGGGTGCAGTTATGCCTCACCTGCTGCACACTCAAAATGCCGTTTTCTT14110.13114394511844316No Hit
GGCCTTTTCCTTCAAACAGGATTTACGGTAGCAGCATCTTGTTCTGTTTG13190.12259309965359781No Hit
CCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG12780.11878239678339501No Hit
CCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA12120.11264809460209291No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA11680.1085585598145582No Hit
CCCCCTCCTCTGTTGCTGTAAACCATGTCGTCAGTGGGTTGGAAAGCCAT11130.10344664133013978No Hit
ATGAGGTACTCGTCGGCATCCACCACATCGTCCATGTCTTCTTCATCCAT11040.10261014557814403No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)