FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008104568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008104568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1075604
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT570275.30185830472925No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA490474.559949572519254No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC324453.0164447138537973No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG304842.834128545449812No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA304112.827341661057415No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC303192.8187883273026135No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG294992.742552091661987No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC282712.628383680239196No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA255032.371039899442546No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT246182.288760547562114No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG211551.9668019085090793No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA209891.9513687193428064No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT209851.9509968352665108No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA175611.6326640659573597No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG172161.6005890643768523No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT169651.5772533385892948No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG168011.5620060914611698No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA167381.5561489172595118No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA159071.4788900004090726No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT150821.4021889096730766No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC150311.397447387700306No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC139381.295830063852496No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG132471.2315870896724073No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT132121.2283331040048195No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA128651.1960721603861644No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG127701.1872399135741407No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT121981.1340604906638503No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC119381.1098880257046273No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG117101.0886906333557704No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG113011.050665486554531No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT109411.017195919687915No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA101060.9395651187611798No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT94540.8789480143249746No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC91150.8474308388589109No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT88840.825954533452832No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG73930.6873347440135961No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG73820.6863120628037828No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG71940.6688335112178831No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC69320.6444751042205125No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC63480.5901800290813348No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTAT60450.5620098103019328No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT55290.5140367644597826No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC50430.46885284918985054No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG47970.44598197849766275No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTATA41920.38973451195793246No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATCTACGACATAT35280.3280017552928401No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGAGAGGCTG32110.29852994224640295No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA28630.2661760276086738No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC26010.24181762061130305No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA23940.22257261966299866No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC21250.19756341553211032No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC21070.1958899371887795No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA20530.19086950215878706No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACAACGTGGTGGAT19170.178225443564732No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG17430.16204848624586743No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGAGCATCTGCCTCACCTCC17360.1613976891123499No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATACTGAGG16910.15721399325402285No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT16680.15507565981532237No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA16430.15275138433847402No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGATGGAT16260.15117087701421714No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCCTTCTCTAGGTG16130.149962253766256No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGAAAAAGTGGATGAGATGT15860.14745203625125977No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGTTCATGGTGGGGGCAGCGCCT15740.14633638402237256No Hit
TCACAATTGCCAGTTAACGTCTTCTTTCTCTCTCTGTCATAGGGACTCTG14900.13852681842016207No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT14680.1364814560005355No Hit
CTGGTGTTGTTGGGCAGTGTTAGGAAAGAGGCAAGGAAAGGTGATAAAAG14330.1332274703329478No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAGGACATGGACGACGTGGTGGAT13920.12941565855091652No Hit
TCAAATCATCCATTGCTTGGTAGCAGAAAGTCAGTCCCATGGAGACCAAG13550.12597573084518093No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTCCATCCTCTCAGGG12330.11463326651816096No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAATGAGAACGCCTCCCT12010.11165819390779505No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGTTTCATCCTCTCAGGG11010.10236109200040164No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)