FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008104574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008104574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180805
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC1903310.526810652360277No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA1876110.376372334835873No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA162458.984817897735129No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT127187.034097508365365No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA115806.404690135781643No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA101705.624844445673515No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC98985.474406128149111No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG72804.026437321976715No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT63223.4965847183429664No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG61283.3892868006968833No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTAT59723.3030060009402398No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG47292.6155250131356986No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA32581.8019413179945245No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT22011.21733359143829No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC10600.5862669727054008No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTTT10370.5735460855617931No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTATAG7610.42089543983850003No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT6140.33959237852935487No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA5200.28760266585547967No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA4960.2743286966621498No Hit
TCTGTATCAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTATA4690.2593954813196538No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT4150.22952905063466167No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACTTTTAT4080.2256574762866071No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAGGACATTATGACA3580.19800337380050329No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT3420.18915406100495008No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT3080.17034927131439948No Hit
GGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGTG2300.12720887143607754No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACCGTTGCACAATATCCTTTTGA2010.11116949199413734No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA2010.11116949199413734No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACTTTTTT1990.11006332789469317No Hit
GTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATCTG1830.10121401509913996No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)