FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008104586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008104586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1346401
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG738805.487221117631375No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT720815.353605649431336No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA583524.333924291500081No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG427523.175279875757668No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC336172.4968044438469668No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA333102.4740029159217793No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA327632.433376089292863No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC315542.343581147072826No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC297732.211302576275567No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT297282.207960332768618No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG287912.1383673957461413No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC286292.126335319121124No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT274052.0354262957321034No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC258081.9168137872743705No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA252211.8732160775281659No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG250751.8623723541500639No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT237411.7632934021885012No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT231351.7182845229615842No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA220691.6391104878858527No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC213591.5863773125539864No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA212321.5769447586565966No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG206241.5317873352738152No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG205131.5235431346233403No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA203421.5108426092969331No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA195171.4495681450028632No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT183511.3629669021339113No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGTTGCTGAGG171661.274954489784247No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGACAGCGCCT167911.24710246055967No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG142511.0584513826118667No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG140901.0464935780647815No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG140471.0432998787136967No Hit
TGTCCTGCTTGCTTACCTCGCTTGGTGCTCCCTGGGGGCAGCTCGTGGTG136441.0133682313070178No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT135301.0049012144227463No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT104510.7762174864694842No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTGTTCATACCAGGACCA97750.726009561787313No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC94560.7023167689269393No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT83920.6232912780070722No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC83740.6219543806042925No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC74260.5515444507245613No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA73160.5433745221520186No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG63330.4703650695446602No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAAATGCTGAGG58100.43152077278611645No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTTAACTTCTGTAACACC56470.4194144240831669No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT55510.41228430460167514No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG50470.3748511773238433No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC47040.3493758545930967No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG41800.3104572857566208No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTCGGAGCTGGTGGC31720.23559103120095723No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA29920.22222205717316013No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTACTT29460.2188055415882787No Hit
GCAGCTTCTGCCATCTCTCTTCTCCTTTTTCTTCAGCCACAGGCTCCCAG26100.1938501234030575No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT25830.19184477729888794No Hit
CAGCCTCTGGCATTCTGGGAGCCTCATCTGGACCTGGGTCTTCAGTGAAC25540.18969088703885395No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG23370.17357384612756527No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCCCTAGGTG19590.14549900066919141No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT18200.1351751818366148No Hit
ATACGGCCAGGCATTGAAGTTTCATGGAAGCCAGCCCCTCAGGGCAACTG14860.11036830780725801No Hit
TCCACAAACAGAACAAGATGCTAAAAAGGTTTGTACTTTACTTTCATTGG14410.10702606430030875No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTAATG14230.10568916689752904No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTAT14220.10561489481959684No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)