FastQCFastQC Report
Wed 12 Jul 2023
EGAF00008107113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008107113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9000000
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG987251.0969444444444445No Hit
GCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCA309070.3434111111111111No Hit
GGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTAC271330.3014777777777778No Hit
GACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCA254880.2832No Hit
GTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCA242550.2695No Hit
CAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAA240150.2668333333333333No Hit
CTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTC235960.26217777777777773No Hit
GCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAG235150.2612777777777778No Hit
GGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTG230370.2559666666666667No Hit
GCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTC223060.24784444444444445No Hit
GCCCAGCCAGGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCGTGG221120.2456888888888889No Hit
GCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCCCCGGGTAA218990.24332222222222222No Hit
GTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGAT217130.24125555555555553No Hit
GTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGC211360.2348444444444444No Hit
GGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACT206090.2289888888888889No Hit
CTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGC203240.22582222222222223No Hit
CTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCCCCGGGTAAA184230.20470000000000002No Hit
CCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAG181260.20140000000000002No Hit
CCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTG172850.19205555555555556No Hit
GCCCTGCCCAACAGGGTCACCGAGAGGACCGTGGACAAGTCCACCGGTAA170920.1899111111111111No Hit
GCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCT168130.18681111111111112No Hit
CCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCGAG167390.18598888888888887No Hit
GCCTCTCCCTGTCCCCGGGTAAATGAGTGCGACGGCCGGCAAGCCCCCGC165880.18431111111111112No Hit
GTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCT162190.1802111111111111No Hit
GCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCCC158940.1766No Hit
GCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACA154450.17161111111111113No Hit
ATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCCCCG153830.17092222222222223No Hit
GGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGC153690.17076666666666665No Hit
GCCACGAGGCCCTGCCGCTGGCCTTCACACAGAAGACCATCGACCGCTTG149540.16615555555555556No Hit
GGCCCATGAGGCCCTGCCCAACAGGGTCACCGAGAGGACCGTGGACAAGT149460.16606666666666667No Hit
GAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGG147980.16442222222222222No Hit
GGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCCCTGCCG146480.16275555555555554No Hit
CCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAGCAGG144220.16024444444444447No Hit
GCCCCCGCTCCCCGGGCTCTCGCGGTCGCACGAGGATGCTTGGCACGTAC143800.1597777777777778No Hit
GCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTG143270.1591888888888889No Hit
GCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCCCCGGGTAAATGAG141860.15762222222222222No Hit
AGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAAC141120.1568No Hit
CCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCC139520.15502222222222223No Hit
AGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAG136300.15144444444444444No Hit
GCTGTGACCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGA134840.14982222222222222No Hit
GTCACCGAGAGGACCGTGGACAAGTCCACCGGTAAACCCACCCTGTACAA134630.14958888888888888No Hit
CTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGG132290.1469888888888889No Hit
CCGTGGACAAGTCCACCGGTAAACCCACCCTGTACAACGTGTCCCTGGTC132240.14693333333333333No Hit
CAACCACTACACGCAGAAGAGCCTCTCCCTGTCCCCGGGTAAATGAGTGC130810.14534444444444444No Hit
CAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATG130050.14450000000000002No Hit
GACAAGTCCACCGGTAAACCCACCCTGTACAACGTGTCCCTGGTCATGTC129700.1441111111111111No Hit
GGAATGGAACACGGGGGAGACCTACACCTGCGTGGTGGCCCATGAGGCCC129380.14375555555555555No Hit
GGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCAC126320.14035555555555554No Hit
AGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTC126070.14007777777777777No Hit
CTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCT125090.13898888888888888No Hit
CTCTATAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGT124870.13874444444444445No Hit
CCCATGAGGCCCTGCCCAACAGGGTCACCGAGAGGACCGTGGACAAGTCC124130.13792222222222222No Hit
GCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACC120460.13384444444444443No Hit
GTGGCCCATGAGGCCCTGCCCAACAGGGTCACCGAGAGGACCGTGGACAA119790.1331No Hit
GCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCT119290.13254444444444444No Hit
CTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGA118780.13197777777777778No Hit
CCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCGCTGTGACCAGCATAC118720.13191111111111112No Hit
CTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAA117820.13091111111111112No Hit
CCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCG117500.13055555555555554No Hit
CTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAGCA115070.12785555555555556No Hit
CTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCGCTGTG114290.1269888888888889No Hit
GCGACGGCCGGCAAGCCCCCGCTCCCCGGGCTCTCGCGGTCGCACGAGGA113360.12595555555555557No Hit
GGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGA112450.12494444444444446No Hit
CCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTC112250.12472222222222222No Hit
CATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGC112030.12447777777777778No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA111510.12390000000000001No Hit
GACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAGCAGGTG111370.12374444444444445No Hit
GGAACACGGGGGAGACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCC110280.12253333333333333No Hit
TGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCCCCGG110180.12242222222222222No Hit
CTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCCCTGC108570.12063333333333333No Hit
CCCCGCTCCCCGGGCTCTCGCGGTCGCACGAGGATGCTTGGCACGTACCC107490.11943333333333332No Hit
GTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATG107130.11903333333333334No Hit
CCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAG106860.11873333333333334No Hit
GGTAAACCCACCCTGTACAACGTGTCCCTGGTCATGTCCGACACAGCTGG106510.11834444444444445No Hit
CACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTT105750.11750000000000001No Hit
CGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGC105440.11715555555555554No Hit
GGCAAGCCCCCGCTCCCCGGGCTCTCGCGGTCGCACGAGGATGCTTGGCA104590.11621111111111111No Hit
GGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTA102630.11403333333333332No Hit
GTCCACCGGTAAACCCACCCTGTACAACGTGTCCCTGGTCATGTCCGACA101630.11292222222222222No Hit
GGCACCACCACCTTCGCTGTGACCAGCATACTGCGCGTGGCAGCCGAGGA101410.11267777777777778No Hit
CAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCT101380.11264444444444445No Hit
GCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGAC101350.11261111111111112No Hit
CAACAGGGTCACCGAGAGGACCGTGGACAAGTCCACCGGTAAACCCACCC100860.11206666666666668No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100780.11197777777777777No Hit
CGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGA100160.1112888888888889No Hit
CGCAGAAGAGCCTCTCCCTGTCCCCGGGTAAATGAGTGCGACGGCCGGCA99870.11096666666666666No Hit
CTTCGCTGTGACCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGG99840.11093333333333333No Hit
GTCCGACACAGCTGGCACCTGCTACTGACCCTGCTGGCCTGCCCACAGGC98850.10983333333333334No Hit
CCACCACCTTCGCTGTGACCAGCATACTGCGCGTGGCAGCCGAGGACTGG98270.1091888888888889No Hit
AGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGC98020.1089111111111111No Hit
GGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCCCCTCCCAT97840.10871111111111112No Hit
CACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGC97840.10871111111111112No Hit
GTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGG97230.10803333333333333No Hit
CAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCAT96950.10772222222222222No Hit
CATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCCCC95650.10627777777777778No Hit
AGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCAC95500.10611111111111111No Hit
CACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAG95350.10594444444444445No Hit
GGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACC95160.10573333333333333No Hit
GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAG94420.10491111111111111No Hit
AGAGCCTCTCCCTGTCCCCGGGTAAATGAGTGCGACGGCCGGCAAGCCCC94150.10461111111111111No Hit
GCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACA94110.10456666666666667No Hit
GGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAGCAGGTGGCA93630.10403333333333334No Hit
GGAGCCCAGCCAGGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCG92820.10313333333333333No Hit
GCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACA92640.10293333333333332No Hit
GGGTAAATGAGTGCGACGGCCGGCAAGCCCCCGCTCCCCGGGCTCTCGCG92640.10293333333333332No Hit
GCTGGCTGCAGGGGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGG92570.10285555555555556No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGCAAGCTCACCGTGGAC92300.10255555555555557Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCA92030.10225555555555556No Hit
TCTATAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTC91570.10174444444444446No Hit
CTATAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCT90610.10067777777777778No Hit
CTGTGACCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGAC90560.10062222222222221No Hit
TCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAG90210.10023333333333334No Hit
GGTAAATGAGTGCGACGGCCGGCAAGCCCCCGCTCCCCGGGCTCTCGCGG90080.10008888888888888No Hit
GGACCGTGGACAAGTCCACCGGTAAACCCACCCTGTACAACGTGTCCCTG90070.10007777777777778No Hit
CCGGCAAGCCCCCGCTCCCCGGGCTCTCGCGGTCGCACGAGGATGCTTGG90020.10002222222222222No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)