FastQCFastQC Report
Wed 12 Jul 2023
EGAF00008107159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008107159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14370084
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTCACTCAGACCCCAAAATTCCAGGTCCTGAAGACAGGACAGAGCAT8894436.18954628240169No Hit
AATTCAGTGACCCAGATGGAAGGGCCAGTGACTCTCTCAGAAGAGGCCTT5601443.8979869567916237No Hit
GGAGTCACACAAAGTCCCACACACCTGATCAAAACGAGAGGACAGCAAGT3608722.5112727246409974No Hit
CTGGCTGTTCTCCAAGGAGTCTGTGCCGAGGTGCAGCTGGTGCAGTCTGG3361642.3393321848362194No Hit
GGAGTCACCCAAAGTCCCACACACCTGATCAAAACGAGAGGACAGCAAGT3209202.233250689418378No Hit
GAGAGTGTGGGGCTGCATCTTCCTACCCTGAGTGTCCAGGAGGGTGACAA2622601.825041523765623No Hit
GGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAGCGAGT2068631.4395392539111116No Hit
GGTGGCAGCTCCCAGATGGGTCCTGTCCCAGGTGCAGCTGCAGGAGTCGG2037871.4181336727050446No Hit
GGATTTTCCTTGCTGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTG2031521.4137147702128952No Hit
GGTGGCGGCTCCCAGATGGGTCCTGTCCCAGGTGCAGCTGCAGGAGTCGG1980281.3780573586069504No Hit
GGCCTCCCATGGGGTGTCCTGTCACAGGTACAGCTGCAGCAGTCAGGTCC1975121.374466565400731No Hit
GGTGTCACTCAGACCCCAAAATTCCGGGTCCTGAAGACAGGACAGAGCAT1911191.3299783077120495No Hit
GGGTTTTCCTCGTTGCTCTTTTAAGAGGTGTCCAGTGTCAGGTGCAGCTG1708911.1892136469070047No Hit
CAGAGAGTGACTCAGCCCGAGAAGCTCCTCTCTGTCTTTAAAGGGGCCCC1681231.1699514073821697No Hit
GGTGTCACTCAGACCCCAAAATTCCGCATCCTGAAGATAGGACAGAGCAT1624751.1306475313575064No Hit
GAACCTGAAGTCACCCAGACTCCCAGCCATCAGGTCACACAGATGGGACA1450271.0092286168960458No Hit
GGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTG1440891.0027011672304769No Hit
CAGTCGGTGACCCAGCTTGACAGCCAAGTCCCTGTCTTTGAAGAAGCCCC1402410.9759233140182061No Hit
CTGGCTGTTCTCCAAGGAGTCTGTGCCGAACTGCAGCTGGTCCAGTCTGG1342080.9339402608920031No Hit
GGAGTTACGCAGACACCAAGACACCTGGTCATGGGAATGACAAATAAGAA1329690.9253181818561395No Hit
GCTGGCTGTAGCTCCAGGTGCTCACTCCCAGGTGCAGCTGGTGCAGTCTG1174080.8170307146430041No Hit
AAGACCACCCAGCCCCCCTCCATGGATTGCGCTGAAGGAAGAGCTGCAAA1108010.7710532520199603No Hit
GGTGGCAGCTCCCAGATGGGTCCTGTCCCAGGTGCAGCTACAGCAGTGGG1071220.7454514531717421No Hit
GGACTCACCATGGAGTTGGGACTGAGCTGGATTTTCCTTTTGGCTATTTT1035230.720406366448519No Hit
GGTGGCGGCTCCCAGATGGGTCCTGTCCCAGGTGCAGCTACAGCAGTGGG1016120.7071079055626953No Hit
CAACAGAAGGAGGTGGAGCAGAATTCTGGACCCCTCAGTGTTCCAGAGGG954120.6639627158755648No Hit
GAAGTTACCCAGACACCAAAACACCTGGTCATGGGAATGACAAATAAGAA945840.6582007453818641No Hit
GGTGGCAGCAGCTACAGGTGTCCAGTCCCAGGTCCAGCTGGTGCAGTCTG901740.627511989491502No Hit
AAGACCACCCAGCCCATCTCCATGGATTGCGCTGAAGGAAGAGCTGCAAA819890.5705533802029271No Hit
CAGCTGCTGGAGCAGAGCCCTCAGTTTCTAAGCATCCAAGAGGGAGAAAA814140.5665520118045239No Hit
CAACAACCAGTGCAGAGTCCTCAAGCCGTGATCCTCCGAGAAGGGGAAGA788210.548507580053116No Hit
GGCCTCCCATGGGGTGTCCTGTCACAGGTACAACTACAACAGTCAGGTCC769500.5354874752297899No Hit
GGATTTTCCTTGCTGCTATTTTAAAAGGTGTCCAGTGTCAGGTGCAGCTG760960.5295445732954658No Hit
CAGTCTGTGACCCAGCTTGACAGCCAAGTCCCTGTCTTTGAAGAAGCCCC736360.5124256754518624No Hit
CAGTCGGTGACCCAGCTTGGCAGCCAAGTCCCTGTCTTTGAAGAAGCCCC697400.4853137949645945No Hit
GGTGGCAGCAGCTACAGGTGTCCACTCCCAGGTTCAGCTGGTGCAGTCTG694970.4836227818849215No Hit
GGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTCAGGTGCAGCTG685140.47678218164904257No Hit
GAAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAACACCAGT666850.46405435069133905No Hit
GGTGGCGGCTCCCAGATGGGTCCTGTCCCAGCTGCAGCTGCAGGAGTCGG631410.43939200355405017No Hit
CCTGCTACTGACTGTCCCGTCCTGGGTCTTGTCCCAGATCACCTTGAAGG627420.43661540183063646No Hit
GGTGGCAGCTCCCAGATGGGTCCTGTCCCAGCTGCAGCTGCAGGAGTCGG622580.4332472934744153No Hit
CAGTCGGTGACCCAGCTTGACAGCCACGTCTCTGTCTCTGAAGGAACCCC601300.4184387509495421No Hit
GGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGA585890.4077150836418214No Hit
GCTGTCGTCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAACCTC577450.4018417707231217No Hit
GGTGGCAGCAGCTACAGGTGTCCACTCCCAGGTGCAGCTGGTGCAGTCTG556060.38695668028106167No Hit
GGTGGCAGCAGCAACAGGTGCCCACTCCCAGGTTCAGCTGGTGCAGTCTG547270.38083980580767657No Hit
CAACGGAAGGAGGTGGAGCAGAATTCTGGACCCCTCAGTGTTCCAGAGGG521290.36276057954845636No Hit
GGCGTCATGCAGAACCCAAGACACCTGGTCAGGAGGAGGGGACAGGAGGC518390.36074249809534864No Hit
CCTGCTACTGACTGTCCCGTCCTGGGTCTTGTCCCAGGTCACCTTGAAGG516310.35929504657036104No Hit
GGATTTTCCTTGCTGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCAGCTG486390.3384740130955393No Hit
CCTGCTACTGACTGTCCCGTCCTGGGTCTTATCCCAGGTCACCTTGAGGG482080.3354747265221275No Hit
CAGTCAGTGGCTCAGCCGGAAGATCAGGTCAACGTTGCTGAAGGGAATCC465480.3239229499284764No Hit
GGTGGCAGCAGCTACAGGTGTCCAGTCCCAGGTCCAGCTGGTGCAATCTG456960.3179939657972772No Hit
GGTGGCAGCAGCTACAGGTGTCCACTCCCAGGTCCAGCTTGTGCAGTCTG453740.31575319949417135No Hit
CAGTCTGTGAGCCAGCATAACCACCACGTAATTCTCTCTGAAGCAGCCTC439110.30557232650832106No Hit
GAGAATGTGGAGCAGCATCCTTCAACCCTGAGTGTCCAGGAGGGAGACAG430210.2993789041177491No Hit
GGAGTTATCCAGTCACCCCGGCACGAGGTGACAGAGATGGGACAAGAAGT418830.29145967413969187No Hit
GGTGGCAGCAGCAACAGGTGCCCACTCCCAGGTGCAGCTGGTGCAGTCTG399920.2783003912851171No Hit
GGAGTTACTCAGTTCCCCAGCCACAGCGTAATAGAGAAGGGCCAGACTGT398410.27724959714918856No Hit
GGAATCACTCAGTCCCCAAAGTACCTGTTCAGAAAGGAAGGACAGAATGT386250.268787572849261No Hit
GGTGGCAGCAGCCACAGGTGCCCACTCCCAGGTTCAGCTGGTGCAGTCTG376160.26176604117275865No Hit
AAGGACCAAGTGTTTCAGCCTTCCACAGTGGCATCTTCAGAGGGAGCTGT370100.2575489468259197No Hit
GGGTTTTCCTCGTTGCTCTTTTAAGAGGTGTCCAGTGTGAGGTGCAGCTG369520.2571453305352982No Hit
CCTGCTGCTGACCATCCCTTCATGGGTCTTGTCCCAGATCACCTTGAAGG348300.24237854141979961No Hit
CAGACAGTCACTCAGTCTCAACCAGAGATGTCTGTGCAGGAGGCAGAGAC346230.24093804879637445No Hit
CAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAGAAGTT336800.23437580462299315No Hit
GGAGTTATCCAGTCACCCCGCCATGAGGTGACAGAGATGGGACAAGAAGT334950.23308840783394166No Hit
GGTGTCACTCAGACCCCAAAGCACCTGATCACAGCAACTGGACAGCGAGT334670.23289355859019337No Hit
GGTGTCACTCAGACCCCAAAATTCCACATCCTGAAGATAGGACAGAGCAT331510.2306945456964622No Hit
CAGTCTGTGACCCAGCTTGACAGCCACGTCTCTGTCTCTGAAGGAACCCC322900.224702931451201No Hit
TGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGA314930.21915668690593598No Hit
CAACAGAAGGAGGTGGAGCAGGATCCTGGACCCTTCAATGTTCCAGAGGG310470.21605301680908753No Hit
GGTGGCAGCAGCCACAGGTGCCCACTCCCAGGTGCAGCTGGTGCAGTCTG307130.21372874368723246No Hit
CAGTCGGTGACCCAGCTTGGCAGCCACGTCTCTGTCTCTGAAGGAACCCC305030.21226737435911996No Hit
GGAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAACACCAGT303640.21130008704194075No Hit
CAACAGGGAGAAGAGGATCCTCAGGCCTTGAGCATCCAGGAGGGTGAAAA297800.20723608852947556No Hit
GGAGTCTCCCAGAACCCCAGACACAAGATCACAAAGAGGGGACAGAATGT295600.20570513018573866No Hit
GCTGGCTGTAGCTCCAGGTGCTCACTCCCAGCTGCAGCTGGTGCAATCTG294110.20466825385293502No Hit
CAGTCGGTGACCCAGCTTGACATCCACATCACTGTCTCTGAAGGAGCCTC292130.2032903913435718No Hit
GGTGGCAGCAGCTACAGGTGTCCACTCCCAGGTGCAGCTGGTGCAATCTG282100.1963106130764441No Hit
CAACGGAAGGAGGTGGAGCAGGATCCTGGACCCTTCAATGTTCCAGAGGG276180.19219094335147935No Hit
GGAGTCTCCCAGTCCCCAAGGTACAAAGTCACAAAGAGGGGACAGGATGT271250.18876020488119624No Hit
GAGCCTGGAGTCAGCCAGACTCCCAGCCATCAGGTCACACAGATGGGACA270900.18851664332651083No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG265550.18479363099060522No Hit
CCTGCTGCTGACCATCCCTTCATGGGTCTTGTCCCAGGTCACCTTGAAGG264530.18408382303123627No Hit
TGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGA256920.17878809894221911No Hit
CCTGCTGCTGACCATCCCTTCCTGGGTCTTGTCCCAGATCACCTTGAAGG256620.17857933189534592No Hit
AAAAACCAAGTGGAGCAGAGTCCTCAGTCCCTGATCATCCTGGAGGGAAA249470.173603717278201No Hit
GCTGGCTGTAGCTCCAGGTGCTCACTCCCAGGTACAACTGGTGCAGTCTG248810.17314442977507993No Hit
GGACAAAACATTGACCAGCCCACTGAGATGACAGCTACGGAAGGTGCCAT248340.17281736140164525No Hit
CCTGCTGCTGACCACCCCTTCATGGGTCTTGTCCCAGATCACCTTGAAGG247870.17249029302821053No Hit
CAAGAACTGGAGCAGAGTCCTCAGTCCTTGATCGTCCAAGAGGGAAAGAA239880.16693013067982065No Hit
GCTGTTTCCCAGACTCCAAAATACCTGGTCACACAGATGGGAAACGACAA239420.1666100212079484No Hit
GGAATCACCCAGAGCCCAAGATACAAGGTCACAGAGACAGGAACACCAGT237910.16555922707201992No Hit
GGTGGCAGCAGCTACAGGTGTCCACTCCCAGGCTCAGCTGGTGCAGTCTG236840.16481462460483876No Hit
GGAGTCACACAAAGTCCCACACACCTGATCAAAACGAGAGGACAGCAAGC236560.16461977536109043No Hit
TGGCTGAGCTGGGTTTTCCTTGTTGCTCTTTTAAGAGGTGTCCAGTGTCA236310.16444580282202942No Hit
CAGTCGGTGACCCAGCTTGACAGCCACGTCTCTGTCTCTGAGGGAGCCCT234400.1631166526236033No Hit
GCTGGCTGTAGCTCCAGGTGCTCACTCCCAGGTGCAGCTGGTGCAGTCCG231860.16134909162674346No Hit
GCTGGCTGTAGCTCCAGGTGCTCACTCCCAGGTGCAGCTGGTACAGTCTG231070.16079933840331065No Hit
AAAAACCAAGTGGAGCAGAGTCCTCAGTCCTTGATCGTCCAAGAGGGAAA228270.15885084596582735No Hit
GGAGTCACCCAAAGTCCCACACACCTGATCAAAACGAGAGGACAGCAAGC219200.1525391222486939No Hit
GGCCTCCCATGGGGTGTCCTGTCACAGGTACAACTGGACCAGTCAGGCCC213690.1487047674877892No Hit
GGTGGCAGCAGCTACAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTG210330.14636657656280924No Hit
CAGAAGGTAACTCAAGCGCAGACTGAAATTTCTGTGGTGGAGAAGGAGGA209680.1459142479612506No Hit
GAAGACCAGGTGACGCAGAGTCCCGAGGCCCTGAGACTCCAGGAGGGAGA203980.14194767407065956No Hit
GGTGGCGGCTCCCAGATGGGTCCTGTCTCAGGTGCAGCTGCAGGAGTCGG203780.14180849603941076No Hit
GGTGGCAGCAGCAACAGGTGCCCACTCCCAGGTCCAGCTTGTGCAGTCTG203560.14165540020503709No Hit
GGTGGCAGCAGTCACAGATGCCCACTCCCAGGTGCAGCTGGTGCAGTCTG199060.1385238945019389No Hit
GGTGGCAGCAGCAACAGGTGCCCACTCCCAGGTGCAGCTGGTGCAATCTG199000.13848214109256426No Hit
GGAGTCACTCAAACTCCAAGATATCTGATCAAAACGAGAGGACAGCAAGT198360.13803677139256806No Hit
GGTGTCACTCAGACCCCAAAATTCCACGTCCTGAAGACAGGACAGAGCAT197500.1374383058581982No Hit
CCTGCTGCTGACCACCCCTTCCTGGGTCTTGTCCCAGATCACCTTGAAGG195860.13629704600195797No Hit
GGTGGCAGCTCCCAGATGGGTCCTGTCTCAGGTGCAGCTGCAGGAGTCGG194560.13539238879884072No Hit
GGTGGCAGCAGCTACAGGTGTCCAGTCCCAGCTCCAGCTGGTACAATCTG192900.1342372111394756No Hit
CCTGCTGCTGACCATCCCTTCCTGGGTCTTGTCCCAGGTCACCTTGAAGG192400.1338892660613536No Hit
CCTGCTGCTGACCACCCCTTCATGGGTCTTGTCCCAGGTCACCTTGAAGG189840.13210778726136882No Hit
GGTGGCAGCAGCAACAGGTGTCCAGTCCCAGGTCCAGCTGGTGCAGTCTG188980.13150932172699895No Hit
CTGGCTGTTCTCCAAGGAGTCTGTGCCGAAGTGCAGCTGGTGCAGTCTGG188840.1314118971051248No Hit
GGAGTCTCCCAGTCCCCAAGGTACAAAGTCGCAAAGAGAGGACAGGATGT187730.1306394590316939No Hit
GGTGGCAGCAGTCACAGATGCCCACTCCCAGGTTCAGCTGGTGCAGTCTG184350.12828735030358904No Hit
GGCCTCCCATGGGGTGTCCTGTCACAGGTGCAACTGCAGCAGTCAGGTCC182160.12676335086141458No Hit
GGTGTCACTCAGACCCCAAAGTACCTGTTCAGAAAGGAAGGACAGAATGT182000.12665200843641553No Hit
GGTGTCACTCAGACCCCAAAATTCCAGGTCCTAAAGACAGGACAGAGCAT180830.12583781695361002No Hit
GGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCAGCTG175540.12215655802707903No Hit
GGGTTTTCCTCGTTGCTCTTTTAAGAGGTGTCCAGTGTCAGGTGCAACTG173860.12098746256458905No Hit
GGTGGCAGCAGCTACAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTCTG168030.11693042295368627No Hit
TGGCTGAGCTGGGTTTCCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGA166820.11608839586463099No Hit
GGAGTTATCCAGTCACCCCGGCATGAGGTGACAGAGATGGGACAAGAAGT165850.11541338241307428No Hit
CAACAGAAGGAGGTGGAGCAGAATTCTGGACCCTTCAATGTTCCAGAGGG165640.11526724548026303No Hit
TGGCTGAGCTGGGTTTCCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGA157900.10988105567093413No Hit
GCTGGCTGTAGCTCCAGGTGCTCACTCCCAGGTGCAGCTGGTGCAGTCGG156600.1089763984678169No Hit
GGTGGCAGCAGCCACAGGTGCCCACTCCCAGGTCCAGCTTGTGCAGTCTG151140.10517683821472443No Hit
GGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTG150450.10469667400691604No Hit
CCTGCTGCTGACCACCCCTTCCTGGGTCTTGTCCCAGGTCACCTTGAAGG149030.10370850998504949No Hit
GGTGGCAGCAGTCACAGATGGGTCCTGTCCCAGGTGCAGCTGCAGGAGTC147360.10254637342412194No Hit
GCTGGCTGTAGCTCCAGGTGCTCACTCCCAGGTTCAGCTGGTGCAGTCTG147350.1025394145225595No Hit
CAACAGAAGGAGGTGGAGCAGAATTCTGAACCCCTCAGTGTTCCAGAGGG146160.10171130523662911No Hit
GGAGTCTCCCAGTCCCCTAGGTACAAAGTCACAAAGAGGGGACAGGATGT145700.10139119576475684No Hit
CTGGCTGTTCTCCAAGGAGTCTGTGCCGAGGTGCAGTTGGTGCAGTCTGG144830.1007857713288245No Hit
GGACTCACCATGGAGTTGGGACTGAGCTGGATTTTCCTTCTGGCTATTTT144490.10054916867570154No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)