FastQCFastQC Report
Wed 12 Jul 2023
EGAF00008107161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008107161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18641652
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTCACTCAGACCCCAAAATTCCAGGTCCTGAAGACAGGACAGAGCAT9289804.983356625260464No Hit
AATTCAGTGACCCAGATGGAAGGGCCAGTGACTCTCTCAGAAGAGGCCTT7126653.8229712688553565No Hit
CTGGCTGTTCTCCAAGGAGTCTGTGCCGAGGTGCAGCTGGTGCAGTCTGG5069762.7195872983789204No Hit
GAGAGTGTGGGGCTGCATCTTCCTACCCTGAGTGTCCAGGAGGGTGACAA5025402.695791124091363No Hit
GGAGTCACACAAAGTCCCACACACCTGATCAAAACGAGAGGACAGCAAGT4650092.4944624006498994No Hit
GGAGTCACCCAAAGTCCCACACACCTGATCAAAACGAGAGGACAGCAAGT4049752.1724201267140915No Hit
GGTGGCAGCTCCCAGATGGGTCCTGTCCCAGGTGCAGCTGCAGGAGTCGG3365681.8054623055939463No Hit
GGCCTCCCATGGGGTGTCCTGTCACAGGTACAGCTGCAGCAGTCAGGTCC3300371.7704278569302765No Hit
GGTGGCGGCTCCCAGATGGGTCCTGTCCCAGGTGCAGCTGCAGGAGTCGG3185801.7089687115712706No Hit
GGATTTTCCTTGCTGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTG2813821.5094263105008074No Hit
GGTGTCACTCAGACCCCAAAATTCCGGGTCCTGAAGACAGGACAGAGCAT2748391.4743274898598042No Hit
GGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAGCGAGT2745301.4726699114434707No Hit
GGGTTTTCCTCGTTGCTCTTTTAAGAGGTGTCCAGTGTCAGGTGCAGCTG2132671.14403487416244No Hit
GCTGGCTGTAGCTCCAGGTGCTCACTCCCAGGTGCAGCTGGTGCAGTCTG2123151.1389280306273284No Hit
GGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTG1957911.0502878178393202No Hit
GGTGGCAGCTCCCAGATGGGTCCTGTCCCAGGTGCAGCTACAGCAGTGGG1929301.0349404655767633No Hit
GGTGTCACTCAGACCCCAAAATTCCGCATCCTGAAGATAGGACAGAGCAT1927451.0339480642595409No Hit
GAACCTGAAGTCACCCAGACTCCCAGCCATCAGGTCACACAGATGGGACA1885291.011332042889761No Hit
AAGACCACCCAGCCCCCCTCCATGGATTGCGCTGAAGGAAGAGCTGCAAA1778630.9541160837033112No Hit
GGTGGCGGCTCCCAGATGGGTCCTGTCCCAGGTGCAGCTACAGCAGTGGG1713560.919210378994308No Hit
GGTGGCAGCAGCTACAGGTGTCCAGTCCCAGGTCCAGCTGGTGCAGTCTG1611040.8642152530258583No Hit
GGAGTTACGCAGACACCAAGACACCTGGTCATGGGAATGACAAATAAGAA1579880.847499996244968No Hit
CAGTCGGTGACCCAGCTTGACAGCCAAGTCCCTGTCTTTGAAGAAGCCCC1558490.8360256912853004No Hit
GGACTCACCATGGAGTTGGGACTGAGCTGGATTTTCCTTTTGGCTATTTT1507570.8087105155701866No Hit
CCTGCTACTGACTGTCCCGTCCTGGGTCTTGTCCCAGATCACCTTGAAGG1377990.7391995087130689No Hit
GGTGGCAGCAGCTACAGGTGTCCACTCCCAGGTTCAGCTGGTGCAGTCTG1371030.7354659340277353No Hit
AAGACCACCCAGCCCATCTCCATGGATTGCGCTGAAGGAAGAGCTGCAAA1368320.7340122002062908No Hit
GGATTTTCCTTGCTGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCAGCTG1002430.5377366769854947No Hit
GGATTTTCCTTGCTGCTATTTTAAAAGGTGTCCAGTGTCAGGTGCAGCTG990060.5311009989887162No Hit
GAAGTTACCCAGACACCAAAACACCTGGTCATGGGAATGACAAATAAGAA971860.5213379157598264No Hit
GAAGACCAGGTGACGCAGAGTCCCGAGGCCCTGAGACTCCAGGAGGGAGA920160.49360432219204603No Hit
GGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTCAGGTGCAGCTG865910.46450282410593224No Hit
CAGTCTGTGACCCAGCTTGACAGCCAAGTCCCTGTCTTTGAAGAAGCCCC812980.4361094177704851No Hit
CAGTCGGTGACCCAGCTTGACAGCCACGTCTCTGTCTCTGAGGGAGCCCT809270.4341192508045961No Hit
GGAGTTACTCAGTTCCCCAGCCACAGCGTAATAGAGAAGGGCCAGACTGT777750.4172108780917056No Hit
GGAGTTATCCAGTCACCCCGGCACGAGGTGACAGAGATGGGACAAGAAGT772360.41431950344314983No Hit
GGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGA769170.4126082817123718No Hit
GGTGGCAGCAGCAACAGGTGCCCACTCCCAGGTTCAGCTGGTGCAGTCTG758710.4069971910214824No Hit
CCTGCTGCTGACCATCCCTTCATGGGTCTTGTCCCAGATCACCTTGAAGG754800.4048997374267045No Hit
AATTCAGTGACCCAGATGGAAGGGCCAGTGACTCTCTCAGAAGAGACCTT752790.403821506806371No Hit
CAGTCGGTGACCCAGCTTGGCAGCCAAGTCCCTGTCTTTGAAGAAGCCCC736940.39531904146692576No Hit
CCTGCTACTGACTGTCCCGTCCTGGGTCTTATCCCAGGTCACCTTGAGGG719740.38609239138248047No Hit
GGTGGCAGCTCCCAGATGGGTCCTGTCCCAGCTGCAGCTGCAGGAGTCGG718750.38556132256948045No Hit
GGTGGCAGCAGCTACAGGTGTCCACTCCCAGGTGCAGCTGGTGCAGTCTG707580.37956936434603544No Hit
CAGAGAGTGACTCAGCCCGAGAAGCTCCTCTCTGTCTTTAAAGGGGCCCC692720.37159796781959026No Hit
GCTGTCGTCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAACCTC690230.37026224928992346No Hit
GGTGGCGGCTCCCAGATGGGTCCTGTCCCAGCTGCAGCTGCAGGAGTCGG687960.36904454605203446No Hit
GAAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAACACCAGT627440.3365796121502536No Hit
AAGACCACCCAGCCCATCTCCATGGACTCATATGAAGGACAAGAAGTGAA610900.3277070079411417No Hit
GCTGGCTGTAGCTCCAGGTGCTCACTCCCAGGTGCAGCTGATGCAGTCTG600630.32219783954769676No Hit
CAGTCGGTGACCCAGCTTGACAGCCACGTCTCTGTCTCTGAAGGAACCCC580990.3116622925908069No Hit
CAGTCAGTGGCTCAGCCGGAAGATCAGGTCAACGTTGCTGAAGGGAATCC573830.3078214312765843No Hit
GGCGTCATGCAGAACCCAAGACACCTGGTCAGGAGGAGGGGACAGGAGGC549030.2945178892943608No Hit
CCTGCTGCTGACCATCCCTTCCTGGGTCTTGTCCCAGATCACCTTGAAGG547220.2935469453029163No Hit
CCTGCTGCTGACCACCCCTTCATGGGTCTTGTCCCAGATCACCTTGAAGG544830.2922648700876939No Hit
GCTGGCTGTAGCTCCAGGTGCTCACTCCCAGGTGCACCTGGTGCAGTCTG540950.2901835094872493No Hit
CAGTCGGTGACCCAGCTTGACATCCACATCACTGTCTCTGAAGGAGCCTC538540.28889070560913804No Hit
GGTGGCAGCAGCCACAGGTGCCCACTCCCAGGTTCAGCTGGTGCAGTCTG525610.28195462505147073No Hit
TGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGA507850.2724275724061366No Hit
GGAGTCTCCCAGAACCCCAGACACAAGATCACAAAGAGGGGACAGAATGT486060.260738694188691No Hit
CAGTCGGTGACCCAGCTTGGCAGCCACGTCTCTGTCTCTGAGGGAGCCCT481680.2583891170160241No Hit
ATACAAGTGGAGCAGAGTCCTCCAGACCTGATTCTCCAGGAGGGAGCCAA479230.25707485581213513No Hit
GGTGTCACTCAGACCCCAAAGCACCTGATCACAGCAACTGGACAGCGAGT474310.2544356047414682No Hit
GGGTTTTCCTCGTTGCTCTTTTAAGAGGTGTCCAGTGTGAGGTGCAGCTG471670.2530194212401347No Hit
TGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGA464430.24913564527435658No Hit
CAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAGAAGTT461490.24755853182968976No Hit
AATTCAGTCAAGCAGACGGGCCAAATAACCGTCTCGGAGGGAGCATCTGT449960.24137345767424476No Hit
CAAAAGATAGAACAGAATTCCGAGGCCCTGAACATTCAGGAGGGTAAAAC448040.24034350603691132No Hit
GGAGTTATCCAGTCACCCCGCCATGAGGTGACAGAGATGGGACAAGAAGT439330.23567117334879978No Hit
CAGTCTGTGACCCAGCTTGACAGCCACGTCTCTGTCTCTGAGGGAGCCCT434750.23321430954724398No Hit
CCTGCTGCTGACCACCCCTTCCTGGGTCTTGTCCCAGATCACCTTGAAGG425950.2284936978761324No Hit
GGTGTCACTCAGACCCCAAAATTCCACATCCTGAAGACAGGACAGAGCAT410110.21999659686813164No Hit
GGTGGCAGCAGCAACAGGTGCCCACTCCCAGGTGCAGCTGGTGCAGTCTG401040.21513114824802007No Hit
CAGTCTGTGAGCCAGCATAACCACCACGTAATTCTCTCTGAAGCAGCCTC396940.21293177235579766No Hit
CAACAGAAGGAGGTGGAGCAGAATTCTGGACCCCTCAGTGTTCCAGAGGG392890.21075921812079745No Hit
GGAATCACTCAGTCCCCAAAGTACCTGTTCAGAAAGGAAGGACAGAATGT390520.20948787156846402No Hit
GCTGTTTCCCAGACTCCAAAATACCTGGTCACACAGATGGGAAACGACAA387080.2076425415515749No Hit
GGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCAGCTG384050.20601714912390812No Hit
GGTGTCACTCAGACCCCAAAATTCCACATCCTGAAGATAGGACAGAGCAT383520.20573283955735255No Hit
GGCCTCCCATGGGGTGTCCTGTCACAGGTACAGCTGCAACAGTCAGGCCC377030.20225138844990778No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG368510.19768097805924068No Hit
GAGCCTGGAGTCAGCCAGACTCCCAGCCATCAGGTCACACAGATGGGACA354860.1903586656375733No Hit
GGTGGCAGCAGCTACAGGTGTCCACTCCCAGGTGCAGCTGGTGCAATCTG353380.18956474458379546No Hit
CAGACAGTCACTCAGTCTCAACCAGAGATGTCTGTGCAGGAGGCAGAGAC349760.1876228566009064No Hit
GGTGGCAGCAGCAACAGGTGCCCACTCCCAGGTGCAGCTGGTGCAATCTG344780.18495141954157282No Hit
AAGGACCAAGTGTTTCAGCCTTCCACAGTGGCATCTTCAGAGGGAGCTGT343960.18451154436312833No Hit
CAACAGAAGGAGGTGGAGCAGGATCCTGGACCACTCAGTGTTCCAGAGGG335790.18012888557301682No Hit
GGTGGCAGCAGCAACAGGTGTCCAGTCCCAGGTCCAGCTGGTGCAGTCTG334060.17920085623312784No Hit
GGTGGCAGCAGTCACAGATGCCCACTCCCAGGTTCAGCTGGTGCAGTCTG321370.17239351963012722No Hit
GGTGGCAGCAGCTACAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTG317430.1702799730410159No Hit
TGGCTGAGCTGGGTTTCCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGA315250.1691105487861269No Hit
CAGAAGGTAACTCAAGCGCAGACTGAAATTTCTGTGGTGGAGAAGGAGGA311960.16734568374090453No Hit
CAGTCTGTGACCCAGCTTGACAGCCACGTCTCTGTCTCTGAAGGAACCCC309210.16587049259368217No Hit
CAACGGAAGGAGGTGGAGCAGGATCCTGGACCACTCAGTGTTCCAGAGGG308740.16561836901579324No Hit
GGTGGCAGCAGCTACAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTCTG305180.16370866702157083No Hit
GGTGGCAGCAGCCACAGGTGCCCACTCCCAGGTGCAGCTGGTGCAGTCTG303040.16256070009245963No Hit
GGCCTCCCATGGGGTGTCCTGTCACAGGTACAGCTGCGCCAGTCAGGTTC299320.16056516879512608No Hit
TGGCTGAGCTGGGTTTTCCTTGTTGCTCTTTTAAGAGGTGTCCAGTGTCA294930.15821022729101478No Hit
GGAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAACACCAGT293560.1574753138831258No Hit
GGTGGCAGCAGCTACAGGTGTCCAGTCCCAGGTCCAGCTGGTGCAATCTG293230.1572982909454591No Hit
CAGTCGGTGACCCAGCTTGGCAGCCACGTCTCTGTCTCTGAAGGAACCCC281970.15125805373901413No Hit
GCTGGCTGTAGCTCCAGGTGCTCACTCCCAGGTTCAGCTGGTGCAGTCTG267520.14350659480179118No Hit
GGTGGCAGCAGCTACAGGTGTCCAGTCCCAGGTCCATCTGGGGCAATCTG261650.140357732243902No Hit
GGATTTTCCTTGCTGCTATTTTAAAAGGTGTCCAGTGCGAGGTGCAACTG258220.13851776655845738No Hit
GGTGGCAGCTCCCAGATGGGTCCTGTCTCAGGTGCAGCTGCAGGAGTCGG256010.13733224930923504No Hit
GGTGGCAGCAGTCACAGATGGGTCCTGTCCCAGGTGCAGCTGCAGGAGTC255350.13697820343390169No Hit
GGTGGCAGCAGCTACAGGTGTCCACTCCCAGGTCCAGCTTGTGCAGTCTG248390.133244628748568No Hit
GCTGGCTGTAGCTCCAGGTGCTCACTCCCAGGTGCAGCTGGAGCAGTCTG248250.13316952810834576No Hit
AAAAACCAAGTGGAGCAGAGTCCTCAGTCCCTGATCATCCTGGAGGGAAA247580.13281011790156796No Hit
ATGGAACTGGGGCTCCGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGT247340.13268137394690127No Hit
TGGCTGAGCTGGGTTTCCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGA247230.13262236630101237No Hit
GGAGTCACTCAAACTCCAAGATATCTGATCAAAACGAGAGGACAGCAAGT245100.1314797637033456No Hit
GGTGGCGGCTCCCAGATGGGTCCTGTCTCAGGTGCAGCTGCAGGAGTCGG241570.12958615470345652No Hit
GGTGGCAGCAGCCACAGGTGCCCACTCCCAGGTGCAGCTGGTGCAATCTG241140.12935548845134542No Hit
GGAATCACCCAGAGCCCAAGATACAAGGTCACAGAGACAGGAACACCAGT238080.12771400302934527No Hit
GGTGGCAGCAGCAACAGGTGTCCACTCCCAGGTTCAGCTGGTGCAGTCTG237360.12732777116534522No Hit
AGAGTCACCCAGACACCAAGGCACAAGGTGACAGAGATGGGACAAGAAGT235130.12613152525323398No Hit
AAAGTCACACAGACTCCAGGACATTTGGTCAAAGGAAAAGGACAGAAAAC234520.12580430103512286No Hit
GAAGACAAGGTGGTACAAAGCCCTCTATCTCTGGTTGTCCACGAGGGAGA230230.12350300284545597No Hit
GGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTG227880.12224238495601142No Hit
GGTGGCAGCAGTCACAGATGCCCACTCCCAGGTGCAGCTGGTGCAGTCTG227270.12191516073790026No Hit
GCCAAAAATGAAGTGGAGCAGAGTCCTCAGAACCTGACTGCCCAGGAAGG220570.11832105867012216No Hit
GGACTCACCATGGAGTTGGGACTGAGCTGGATTTTCCTTCTGGCTATTTT218750.11734475034723317No Hit
CAGTCGGTGACCCAGCTTGACAGCCACATCACTGTCTCTGAAGGAGCCTC218370.11714090575234426No Hit
CAGTCGGTGACCCAGCTTGGCATCCACATCACTGTCTCTGAAGGAGCCTC217520.11668493757956645No Hit
GAGAATGTGGAGCAGCATCCTTCAACCCTGAGTGTCCAGGAGGGAGACAG216920.11636307769289975No Hit
CAACAGAAGGAGGTGGAGCAGAATTCTGGACCACTCAGTGTTCCAGAGGG213120.11432463174401067No Hit
GGAGTCTCCCAGTCCCCCAGTAACAAGGTCACAGAGAAGGGAAAGGATGT212630.11406177950323286No Hit
CAACGGAAGGAGGTGGAGCAGAATTCTGGACCCCTCAGTGTTCCAGAGGG208460.11182485329089932No Hit
GGTGGCAGCAGCTACAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGTCTG205790.11039257679523251No Hit
CAGTCAGTGACCCAGCCTGACATCCACATCACTGTCTCTGAAGGAGCCTC199920.10724371423734334No Hit
GGAGTTTGGGCTGAGCTGGGTTTTCCTCGTTGCTCTTTTAAGAGGTGTCC192750.10339748859167631No Hit
CAGCTGCTGGAGCAGAGCCCTCAGTTTCTAAGCATCCAAGAGGGAGAAAA191900.10294152041889851No Hit
GGTGTCACTCAGACCCCAAAGTACCTGTTCAGAAAGGAAGGACAGAATGT191050.10248555224612067No Hit
GGAGTTATCCAGTCACCCCGGCATGAGGTGACAGAGATGGGACAAGAAGT190460.10216905669089842No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)