FastQCFastQC Report
Wed 12 Jul 2023
EGAF00008107163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008107163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19050495
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTCACTCAGACCCCAAAATTCCAGGTCCTGAAGACAGGACAGAGCAT11454646.012778145659732No Hit
AATTCAGTGACCCAGATGGAAGGGCCAGTGACTCTCTCAGAAGAGGCCTT7809574.0994052910436185No Hit
CTGGCTGTTCTCCAAGGAGTCTGTGCCGAGGTGCAGCTGGTGCAGTCTGG4505652.365109148082504No Hit
GGAGTCACACAAAGTCCCACACACCTGATCAAAACGAGAGGACAGCAAGT4396502.3078140489262875No Hit
GGAGTCACCCAAAGTCCCACACACCTGATCAAAACGAGAGGACAGCAAGT3932422.06420883026924No Hit
GAGAGTGTGGGGCTGCATCTTCCTACCCTGAGTGTCCAGGAGGGTGACAA3807461.9986147341578264No Hit
CAGAGAGTGACTCAGCCCGAGAAGCTCCTCTCTGTCTTTAAAGGGGCCCC2731611.4338787522318974No Hit
GGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAGCGAGT2682181.4079319198792473No Hit
GGCCTCCCATGGGGTGTCCTGTCACAGGTACAGCTGCAGCAGTCAGGTCC2678271.405879479772048No Hit
GGATTTTCCTTGCTGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTG2473711.2985016924757073No Hit
GGTGGCAGCTCCCAGATGGGTCCTGTCCCAGGTGCAGCTGCAGGAGTCGG2441251.2814627651407484No Hit
GGTGTCACTCAGACCCCAAAATTCCGGGTCCTGAAGACAGGACAGAGCAT2378511.248529237691724No Hit
GGTGGCGGCTCCCAGATGGGTCCTGTCCCAGGTGCAGCTGCAGGAGTCGG2323381.2195903571009572No Hit
CAGTCGGTGACCCAGCTTGACAGCCAAGTCCCTGTCTTTGAAGAAGCCCC2089841.0970003666571395No Hit
GGTGTCACTCAGACCCCAAAATTCCGCATCCTGAAGATAGGACAGAGCAT2005891.05293327023786No Hit
GGGTTTTCCTCGTTGCTCTTTTAAGAGGTGTCCAGTGTCAGGTGCAGCTG1965201.0315742451836554No Hit
GAACCTGAAGTCACCCAGACTCCCAGCCATCAGGTCACACAGATGGGACA1916121.0058111350912404No Hit
GGAGTTACGCAGACACCAAGACACCTGGTCATGGGAATGACAAATAAGAA1906811.0009241229689831No Hit
AAGACCACCCAGCCCCCCTCCATGGATTGCGCTGAAGGAAGAGCTGCAAA1859650.9761688607041444No Hit
GGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTG1714050.8997404004462877No Hit
CAGCTGCTGGAGCAGAGCCCTCAGTTTCTAAGCATCCAAGAGGGAGAAAA1649080.8656362997391932No Hit
GGTGGCAGCTCCCAGATGGGTCCTGTCCCAGGTGCAGCTACAGCAGTGGG1597060.8383299226608023No Hit
GCTGGCTGTAGCTCCAGGTGCTCACTCCCAGGTGCAGCTGGTGCAGTCTG1589190.8341987964092271No Hit
CAACAGAAGGAGGTGGAGCAGAATTCTGGACCCCTCAGTGTTCCAGAGGG1489690.7819691824280682No Hit
GGTGGCGGCTCCCAGATGGGTCCTGTCCCAGGTGCAGCTACAGCAGTGGG1454550.7635234675004507No Hit
AAGACCACCCAGCCCATCTCCATGGATTGCGCTGAAGGAAGAGCTGCAAA1432380.7518859746164076No Hit
CTGGCTGTTCTCCAAGGAGTCTGTGCCGAACTGCAGCTGGTCCAGTCTGG1356400.7120024965230562No Hit
GAAGTTACCCAGACACCAAAACACCTGGTCATGGGAATGACAAATAAGAA1331080.6987115032968959No Hit
GGACTCACCATGGAGTTGGGACTGAGCTGGATTTTCCTTTTGGCTATTTT1317450.6915568335625925No Hit
CAACAACCAGTGCAGAGTCCTCAAGCCGTGATCCTCCGAGAAGGGGAAGA1269120.6661874140278244No Hit
CAGTCGGTGACCCAGCTTGACAGCCACGTCTCTGTCTCTGAAGGAACCCC1247850.6550223498129576No Hit
CAGTCTGTGACCCAGCTTGACAGCCAAGTCCCTGTCTTTGAAGAAGCCCC1109410.5823523220787701No Hit
CAGTCGGTGACCCAGCTTGGCAGCCAAGTCCCTGTCTTTGAAGAAGCCCC1036010.5438231395037242No Hit
GGTGGCAGCAGCTACAGGTGTCCAGTCCCAGGTCCAGCTGGTGCAGTCTG1019830.5353299218734211No Hit
CAGTCAGTGGCTCAGCCGGAAGATCAGGTCAACGTTGCTGAAGGGAATCC931700.4890686567461895No Hit
CCTGCTACTGACTGTCCCGTCCTGGGTCTTGTCCCAGATCACCTTGAAGG931280.4888481900339072No Hit
CAGTCTGTGAGCCAGCATAACCACCACGTAATTCTCTCTGAAGCAGCCTC920620.4832525349078856No Hit
GGATTTTCCTTGCTGCTATTTTAAAAGGTGTCCAGTGTCAGGTGCAGCTG915260.4804389597225689No Hit
GAAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAACACCAGT907040.47612411121075854No Hit
CAACGGAAGGAGGTGGAGCAGAATTCTGGACCCCTCAGTGTTCCAGAGGG829110.4352170376675252No Hit
AAGGACCAAGTGTTTCAGCCTTCCACAGTGGCATCTTCAGAGGGAGCTGT820980.43094943202263253No Hit
GGCCTCCCATGGGGTGTCCTGTCACAGGTACAACTACAACAGTCAGGTCC817660.42920669515411536No Hit
GGAGTTATCCAGTCACCCCGGCACGAGGTGACAGAGATGGGACAAGAAGT813640.4270965137651279No Hit
GGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTCAGGTGCAGCTG809350.4248446037753875No Hit
GCTGTCGTCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAACCTC798080.41892874699581295No Hit
CAGACAGTCACTCAGTCTCAACCAGAGATGTCTGTGCAGGAGGCAGAGAC713630.37459919020476895No Hit
GGTGGCAGCAGCTACAGGTGTCCACTCCCAGGTGCAGCTGGTGCAGTCTG704210.3696544368007235No Hit
GGTGGCAGCTCCCAGATGGGTCCTGTCCCAGCTGCAGCTGCAGGAGTCGG696830.36578052171347775No Hit
GGATTTTCCTTGCTGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCAGCTG693040.363791072095502No Hit
GGTGGCAGCAGCTACAGGTGTCCACTCCCAGGTTCAGCTGGTGCAGTCTG685330.35974393316289155No Hit
GGTGGCGGCTCCCAGATGGGTCCTGTCCCAGCTGCAGCTGCAGGAGTCGG680960.35745002951366883No Hit
CAGTCTGTGACCCAGCTTGACAGCCACGTCTCTGTCTCTGAAGGAACCCC674660.3541430288294346No Hit
GGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGA672380.3529462095341879No Hit
GAGAATGTGGAGCAGCATCCTTCAACCCTGAGTGTCCAGGAGGGAGACAG664420.34876784041569525No Hit
CCTGCTACTGACTGTCCCGTCCTGGGTCTTGTCCCAGGTCACCTTGAAGG650370.3413927039691095No Hit
CAACAGAAGGAGGTGGAGCAGGATCCTGGACCCTTCAATGTTCCAGAGGG643540.3378074952908048No Hit
CAGTCGGTGACCCAGCTTGGCAGCCACGTCTCTGTCTCTGAAGGAACCCC637030.3343902612504294No Hit
AAAAACCAAGTGGAGCAGAGTCCTCAGTCCCTGATCATCCTGGAGGGAAA623460.32726708676073774No Hit
CCTGCTACTGACTGTCCCGTCCTGGGTCTTATCCCAGGTCACCTTGAGGG621270.3261175103324087No Hit
GGTGGCAGCAGCTACAGGTGTCCACTCCCAGGTCCAGCTTGTGCAGTCTG613770.3221806047559394No Hit
GGAGTTATCCAGTCACCCCGCCATGAGGTGACAGAGATGGGACAAGAAGT605700.31794449435565847No Hit
GGCGTCATGCAGAACCCAAGACACCTGGTCAGGAGGAGGGGACAGGAGGC592330.3109263040146726No Hit
CAACAGGGAGAAGAGGATCCTCAGGCCTTGAGCATCCAGGAGGGTGAAAA582070.3055406171860626No Hit
CAACGGAAGGAGGTGGAGCAGGATCCTGGACCCTTCAATGTTCCAGAGGG557360.2925698256134552No Hit
GGAATCACTCAGTCCCCAAAGTACCTGTTCAGAAAGGAAGGACAGAATGT534070.2803444214966593No Hit
CAGTCGGTGACCCAGCTTGACAGCCACGTCTCTGTCTCTGAGGGAGCCCT507660.2664812646600521No Hit
CCTGCTGCTGACCATCCCTTCATGGGTCTTGTCCCAGATCACCTTGAAGG504410.26477527224358216No Hit
GGTGGCAGCAGCAACAGGTGCCCACTCCCAGGTTCAGCTGGTGCAGTCTG502410.26372543075652366No Hit
GGAGTTACTCAGTTCCCCAGCCACAGCGTAATAGAGAAGGGCCAGACTGT467540.24542144442965919No Hit
CAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAGAAGTT452940.23775760157413234No Hit
GGTGGCAGCAGCAACAGGTGCCCACTCCCAGGTGCAGCTGGTGCAGTCTG444450.23330102446156908No Hit
GGTGTCACTCAGACCCCAAAGCACCTGATCACAGCAACTGGACAGCGAGT438900.23038771433498184No Hit
CAGTCGGTGACCCAGCTTGACATCCACATCACTGTCTCTGAAGGAGCCTC427450.2243773718215721No Hit
GGACAAAACATTGACCAGCCCACTGAGATGACAGCTACGGAAGGTGCCAT419550.2202304979476911No Hit
GGAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAACACCAGT418530.2196950787892913No Hit
GGGTTTTCCTCGTTGCTCTTTTAAGAGGTGTCCAGTGTGAGGTGCAGCTG414700.21768463234157434No Hit
GGTGTCACTCAGACCCCAAAATTCCACATCCTGAAGATAGGACAGAGCAT410790.21563219223437502No Hit
GGAGTCTCCCAGAACCCCAGACACAAGATCACAAAGAGGGGACAGAATGT398090.20896569879155372No Hit
GGTGGCAGCAGCTACAGGTGTCCAGTCCCAGGTCCAGCTGGTGCAATCTG386050.2026456530394617No Hit
CAGAAGGTAACTCAAGCGCAGACTGAAATTTCTGTGGTGGAGAAGGAGGA381540.20027826048614486No Hit
GGAGTCACACAAAGTCCCACACACCTGATCAAAACGAGAGGACAGCAAGC367880.1931078431295355No Hit
TGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGA367700.19301335739570022No Hit
GGTGGCAGCAGCCACAGGTGCCCACTCCCAGGTTCAGCTGGTGCAGTCTG364920.19155407772868893No Hit
CCTGCTGCTGACCATCCCTTCCTGGGTCTTGTCCCAGATCACCTTGAAGG364650.19141234912793606No Hit
CCTGCTGCTGACCACCCCTTCATGGGTCTTGTCCCAGATCACCTTGAAGG359980.1889609692556545No Hit
GGTGGCAGCAGCCACAGGTGCCCACTCCCAGGTGCAGCTGGTGCAGTCTG352490.18502931288662053No Hit
GAGCCTGGAGTCAGCCAGACTCCCAGCCATCAGGTCACACAGATGGGACA351180.18434166671259725No Hit
CAAGAACTGGAGCAGAGTCCTCAGTCCTTGATCGTCCAAGAGGGAAAGAA349300.18335481571476228No Hit
AAAAACCAAGTGGAGCAGAGTCCTCAGTCCTTGATCGTCCAAGAGGGAAA344180.18066722150789258No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG337920.17738121765339956No Hit
CAACAGAAGGAGGTGGAGCAGAATTCTGGACCCTTCAATGTTCCAGAGGG334580.1756279823700119No Hit
CCTGCTGCTGACCATCCCTTCATGGGTCTTGTCCCAGGTCACCTTGAAGG330610.17354404701820084No Hit
GGAGTCTCCCAGTCCCCAAGGTACAAAGTCACAAAGAGGGGACAGGATGT329960.17320284853490683No Hit
GGAGTCACCCAAAGTCCCACACACCTGATCAAAACGAGAGGACAGCAAGC327980.17216350546271894No Hit
GGAATCACCCAGAGCCCAAGATACAAGGTCACAGAGACAGGAACACCAGT322520.16929743820304932No Hit
GGTGGCAGCAGCTACAGGTGTCCACTCCCAGGTGCAGCTGGTGCAATCTG315160.16543402153067413No Hit
TGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGA310230.162846162265075No Hit
CAGTCGGTGACCCAGCTTGGCAGCCACGTCTCTGTCTCTGAGGGAGCCCT307340.16132914131627552No Hit
GCTGTTTCCCAGACTCCAAAATACCTGGTCACACAGATGGGAAACGACAA299630.1572820023836651No Hit
AAGACCACCCAGCCCATCTCCATGGACTCATATGAAGGACAAGAAGTGAA290690.15258921093651373No Hit
GGCCTCCCATGGGGTGTCCTGTCACAGGTGCAACTGCAGCAGTCAGGTCC286430.15035304856907916No Hit
CCTGCTGCTGACCACCCCTTCCTGGGTCTTGTCCCAGATCACCTTGAAGG280990.14749747972428012No Hit
GAAGACCAGGTGACGCAGAGTCCCGAGGCCCTGAGACTCCAGGAGGGAGA280920.14746073527223308No Hit
CAACAGAAGGAGGTGGAGCAGGATCCTGGACCACTCAGTGTTCCAGAGGG278060.14595946194573947No Hit
GGTGGCAGCAGCTACAGGTGTCCACTCCCAGGCTCAGCTGGTGCAGTCTG276740.14526656656428089No Hit
CAAAAGATAGAACAGAATTCCGAGGCCCTGAACATTCAGGAGGGTAAAAC275900.1448256331397163No Hit
GGAGTTATCCAGTCACCCCGGCATGAGGTGACAGAGATGGGACAAGAAGT274500.1440907440987754No Hit
CAGTCTGTGACCCAGCTTGACAGCCACGTCTCTGTCTCTGAGGGAGCCCT273280.1434503407916697No Hit
TGGCTGAGCTGGGTTTTCCTTGTTGCTCTTTTAAGAGGTGTCCAGTGTCA268550.14096746567477644No Hit
GGTGGCAGCAGCTACAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTG267940.1406472640212236No Hit
GCTGGCTGTAGCTCCAGGTGCTCACTCCCAGCTGCAGCTGGTGCAATCTG265320.139271971673177No Hit
GGTGTCACTCAGACCCCAAAATTCCACGTCCTGAAGACAGGACAGAGCAT265240.13922997801369466No Hit
ATACTGAACGTGGAACAAAGTCCTCAGTCACTGCATGTTCAGGAGGGAGA264200.13868406044042425No Hit
GGTGGCAGCAGCAACAGGTGCCCACTCCCAGGTCCAGCTTGTGCAGTCTG254150.13340860696795542No Hit
GGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCAGCTG252610.1326002290229204No Hit
GGTGTCACTCAGACCCCAAAGTACCTGTTCAGAAAGGAAGGACAGAATGT248890.13064752385699166No Hit
GCTGGCTGTAGCTCCAGGTGCTCACTCCCAGGTGCAGCTGGTACAGTCTG248270.1303220729960035No Hit
GGTGGCAGCAGTCACAGATGCCCACTCCCAGGTGCAGCTGGTGCAGTCTG248220.13029582695882705No Hit
GGCCTCCCATGGGGTGTCCTGTCACAGGTACAACTGGACCAGTCAGGCCC244700.12844810594160413No Hit
CAACGGAAGGAGGTGGAGCAGGATCCTGGACCACTCAGTGTTCCAGAGGG242310.12719354536456928No Hit
GGAGTCACTCAAACTCCAAGATATCTGATCAAAACGAGAGGACAGCAAGT241030.12652164681285183No Hit
CCTGCTGCTGACCATCCCTTCCTGGGTCTTGTCCCAGGTCACCTTGAAGG238340.1251096100127582No Hit
CCTGCTGCTGACCACCCCTTCATGGGTCTTGTCCCAGGTCACCTTGAAGG235370.12355059540447635No Hit
GGTGGCAGCTCCCAGATGGGTCCTGTCTCAGGTGCAGCTGCAGGAGTCGG231730.12163988389802996No Hit
GGTGGCGGCTCCCAGATGGGTCCTGTCTCAGGTGCAGCTGCAGGAGTCGG230630.12106247108014778No Hit
CAACAGAAGGAGGTGGAGCAGAATTCTGAACCCCTCAGTGTTCCAGAGGG230450.12096798534631252No Hit
GGACTCACCATGGAGTTGGGACTGAGCTGGATTTTCCTTCTGGCTATTTT229490.12046406143252446No Hit
GGAGTCTCCCAGTCCCCAAGGTACAAAGTCGCAAAGAGAGGACAGGATGT229200.12031183441690096No Hit
GGTGGCAGCAGCTACAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTCTG219130.11502588252956156No Hit
GCTGGCTGTAGCTCCAGGTGCTCACTCCCAGGTACAACTGGTGCAGTCTG214010.11233828832269187No Hit
GGTGTCACTCAGACCCCAAAATTCCAGGTCCTAAAGACAGGACAGAGCAT212080.11132519128768045No Hit
GGTGGCAGCAGCAACAGGTGTCCAGTCCCAGGTCCAGCTGGTGCAGTCTG209160.10979242271657508No Hit
GGTGGCAGCAGCAACAGGTGCCCACTCCCAGGTGCAGCTGGTGCAATCTG208980.10969793698273982No Hit
TGGCTGAGCTGGGTTTCCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGA200050.10501039474302373No Hit
GGCGTCATGCAGAACCCAAGACACCTGGTCATGGGAATGACAAATAAGAA198170.10402354374518877No Hit
GCTGGCTGTAGCTCCAGGTGCTCACTCCCAGGTGCAGCTGGTGCAGTCGG197300.10356686269831834No Hit
GCTGGCTGTAGCTCCAGGTGCTCACTCCCAGGTGCAGCTGGTGCAGTCCG196860.10333589757116547No Hit
GCTGGCTGTAGCTCCAGGTGCTCACTCCCAGGTGCAGCTGGAGCAGTCTG195580.10266399901944805No Hit
GGTGGCAGCAGCCACAGGTGCCCACTCCCAGGTCCAGCTTGTGCAGTCTG193230.10143043527215435No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)