FastQCFastQC Report
Wed 12 Jul 2023
EGAF00008107185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008107185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9571526
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGA8261478.63129870827285No Hit
TTCAGCAGGCACACAACAGACGCAGTTCCAGATTTCAACTGCTCATCAGA3465823.620969111926353No Hit
GCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAGGCTCAGCGGGAA2601752.718218599625598No Hit
TGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCA2009232.0991741546750227No Hit
TTCAGCAGGCTCACATCAGACGCAGTTCCAGATTTCAACTGCTCATCAGA1811711.8928120761517027No Hit
TGGCTTGAAGCTCCTCAGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA1559831.6296565458841152No Hit
TTCAGCAGGCACACAACAGAGGCAGTTACAGATTTCAACTGCTCATCAGA1359451.4203064380747648No Hit
TGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCA1327681.3871142386281978No Hit
TTCAGCAGGCTCACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGA1302081.360368242221773No Hit
TTCAGCAGGCTCACAACAGACGCAGTTCCAGATTTCAACTGCTCATCAGA1229151.2841734954280017No Hit
GCGACGACCACGTTCCCATCTGGCTGGGTGCTGCAGAGGCTCAGCGGGAA1186681.239802305295937No Hit
TTCAGCAGGCACACAACAGACGCAGTTACAGATTTCAACTGCTCATCAGA1166621.2188443096743404No Hit
TTCAGCAGGCTCACATCAGAGGCAGTTCCAGATTTCAACTGCTCATCAGA1147291.1986489928565205No Hit
TGGCTTGAAGCTCCTCTGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA1062191.109739450114851No Hit
GCGATGACCACGTTCCCATCAGGCTGGGTGCTGCAGAGGCTCAGCGGGAA1058411.1057902365829648No Hit
TGGCTTGGAGCTCCTCAGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA1053311.1004619326113725No Hit
TGGCTTGAAGCTCCTCAGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA1014191.059590706852805No Hit
TGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTG954260.9969779113591709No Hit
TGGCTTGAAGCTCCTCTGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA815440.8519435667833949No Hit
TGGCTTGGAGCTCCTCAGAGCAGGGCGGGAACAGAGTGACAGTGGGGTTG802930.838873550570724No Hit
TTCAGCAGGCTCACATGAGACGCAGTTCCAGATTTCAACTGCTCATCAGA762780.7969262163629917No Hit
TTCAGCAGGCACACATCAGACGCAGTTCCAGATTTCAACTGCTCATCAGA699950.7312836009639424No Hit
TGGCTTGAAGCTCCTCTGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCA696380.7275537881838278No Hit
TGGCTTGGAGCTCCTCAGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA676220.7064913160137684No Hit
TTCAGCAGGCTCACATCAGACGCAGTTACAGATTTCAACTGCTCATCAGA665880.6956884409027358No Hit
TGGCTTGGAGCTCCTCTGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA663360.6930556318814785No Hit
GGGAAGTAGTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGT620050.647806838742328No Hit
GGGAAGTAGTCCTTGACCAGCCAGCCCAGGGCCGCTGTGCCCCCAGAGGT548470.5730225253528016No Hit
TGGCTTGGAGCTCCTCAGAGAAGGGCGGGAACAGAGTGACAGTGGGGTTG544020.5683733189462161No Hit
TTCAGCAGGCACACATCAGAGGCAGTTCCAGATTTCAACTGCTCATCAGA537290.5613420472346834No Hit
TGGCTTGGAGCTCCTCTGAGCAGGGCGGGAACAGAGTGACAGTGGGGTTG513710.5367064771072032No Hit
TGGCTTGGAGCTCCTCTGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA508390.531148324728993No Hit
TTCAGCAGGCACACAACAGAAGCAGTTCCAGATTTCAACTGCTCATCAGA499170.5215155869607416No Hit
GGGAAGTAGTGCTTGTGCAGACAGCCCAGGGCCGCTGTGCCCCCAGAGGT483790.5054470938071944No Hit
GGGAAGTAGTCCTTGTCCAGACAGCCCAGGGCCGCTGTGCCCCCAGAGGT466850.48774876649763055No Hit
TGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTG448290.4683579191029727No Hit
GCGACGACCACGTTCCCATCAGGCTGGGTGCTGCAGAGGCTCAGCGGGAA430610.44988646533478566No Hit
TGGCTTGGAGCTCCTCTGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCA416650.4353015391694073No Hit
TTCAGCAGGCTCACAACAGACGCAGTTACAGATTTCAACTGCTCATCAGA409370.42769564644133024No Hit
TGGCTTGGAGCTCCTCTGAGAAGGGCGGGAACAGAGTGACAGTGGGGTTG406650.42485388432314763No Hit
TTCAGCAGGCTCACATCAGAAGCAGTTCCAGATTTCAACTGCTCATCAGA394710.4123793844367137No Hit
TTCAGCAGGCTCACATGAGAGGCAGTTCCAGATTTCAACTGCTCATCAGA393100.41069731200646586No Hit
GGGAAGTAGTCCTTGACCAGACAGCCCAGGGCCGCTGTGCCCCCAGAGGT392220.409777918379995No Hit
TGGCTTGAAGCTCCTCAGAGAAGGGCGTGAACAGAGTGACCGAGGGGGCA381670.39875564251719114No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC375280.39207959107043117No Hit
TGGCTTGAAGCTCCTCAGAGCAGGGCGGGAACAGAGTGACAGTGGGGTTG364630.38095283865916474No Hit
GGGAAGTAGTGCTTGTCCAGACAGCCCAGGGCCGCTGTGCCCCCAGAGGT363610.3798871778648462No Hit
GGGAAGTAGTCCTTGTCCAGCCAGCCCAGGGCCGCTGTGCCCCCAGAGGT357590.3735976896473979No Hit
TGGCTTGAAGCTCCTGTGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA355820.37174845473960993No Hit
TGGCTTGAAGCTCCTGTGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA353780.369617133150973No Hit
TGGCTTGAAGCTCCTCAGAGTAGGGCGGGAACAGAGTGACCGAGGGGGCA339710.3549172827822857No Hit
GCGATGACCACGTTCCCATCTGGCTGGTTGCTGCAGAGGCTCAGCGGGAA322630.33707268830487425No Hit
TGGCTTGAAGGTCCTGTGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA321890.33629956184625104No Hit
TTCAGCAGGCTCACATCAGAGGCAGTTACAGATTTCAACTGCTCATCAGA313920.3279727809337821No Hit
GCGATGACCACGTTCCCATCAGGCTGGTTGCTGCAGAGGCTCAGCGGGAA305160.31882063528845866No Hit
TGGCTTGGAGCTCCTCTGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTG304440.318068404139528No Hit
TGGCTTGAAGGTCCTGTGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA302560.3161042450284312No Hit
CACGCTGCTCGTATCCGACGCGGAATTCTCACAGGAGACGAGGGGGAAAA298480.3118416018511573No Hit
TTCAGCAGGCTCACATGAGACGCAGTTACAGATTTCAACTGCTCATCAGA291950.30501928323654975No Hit
TTCAGCAGGCACACAACAGAAGCAGTTACAGATTTCAACTGCTCATCAGA285830.29862531847063883No Hit
GTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGA283890.29659847343046447No Hit
TGGCTTGAAGCTCCTCAGAGCAGGGCGTGAACAGAGTGACCGAGGGGGCA281620.29422685578036356No Hit
TTCAGCAGGCACACATCAGACGCAGTTACAGATTTCAACTGCTCATCAGA277940.29038211879694No Hit
TGGCTTGAAGCTCCTCTGAGAAGGGCGTGAACAGAGTGACCGAGGGGGCA274480.2867672302201342No Hit
TTCAGCAGGCTCACAACAGAGGCAGTTACAGATTTCAACTGCTCATCAGA273500.28574336004520073No Hit
GGGAAGTAGTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCTCTCGGAGGT268770.2808016193029199No Hit
GGGAAGTAGTGCTTGTGCAGCCAGCCCAGGGCCGCTGTGCCCCCAGAGGT260730.2724017048065272No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA252570.26387641845197934No Hit
TGGCTTGAAGCTCCTCTGAGCAGGGCGGGAACAGAGTGACAGTGGGGTTG251830.26310329199335614No Hit
TGGCTTGGAGCTCCTCAGAGAAGGGCGTGAACAGAGTGACCGAGGGGGCA251060.2622988225701941No Hit
TGGCTTGAAGCTCCTCAGAGAAGGGCGGGAACAGAGTGACAGTGGGGTTG245600.2565944030241364No Hit
GTCAGCAGGCTCACATGAGACGCAGTTCCAGATTTCAACTGCTCATCAGA236400.24698256056557752No Hit
TTCAGCAGGCACACAACAGATGCAGTTCCAGATTTCAACTGCTCATCAGA232970.24339901495331046No Hit
GTCAGCAGGCTCACATCAGACGCAGTTCCAGATTTCAACTGCTCATCAGA231810.24218708699114436No Hit
GGGAAGTAGTCCTTGACCAGCCAGCCCAGGGCCGCTGTGCTCTCGGAGGT226960.23711997439070845No Hit
GGGAAGTAGTGCTTGTCCAGCCAGCCCAGGGCCGCTGTGCCCCCAGAGGT226240.23636774324177776No Hit
GCGATGACCAGGTTCGGATCAGGCTGGGTGCTGCAGAGGCTCAGCGGGAA226220.2363468479320852No Hit
GTCAGCAGGCACACAACAGACGCAGTTCCAGATTTCAACTGCTCATCAGA224300.2343408982016034No Hit
TGGCTTGGAGCTCCTCAGAGAAGGGCGTGAACAGAGTGACAGTGGGGTTG221160.23106033457987785No Hit
TGGCTTGGAGCTCCTCAGAGTAGGGCGGGAACAGAGTGACCGAGGGGGCA219720.2295558722820165No Hit
TGGCTTGGAGCTCCTGTGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA219220.22903348953970348No Hit
TGGCTTGGAGCTCCTGTGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA218480.22826036308108028No Hit
TTCAGCAGGCTCACAACAGAAGCAGTTCCAGATTTCAACTGCTCATCAGA210930.22037238367215425No Hit
GGGAAGTAGTCCTTGTCCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGT207660.21695600053742736No Hit
TGGCTTGGAGCTCCTGTGAGCAGGGCGGGAACAGAGTGACAGTGGGGTTG204390.21353961740270044No Hit
TGGCTTGGAGCTCCTGTGAGAAGGGCGGGAACAGAGTGACAGTGGGGTTG203580.21269335736015343No Hit
TTCAGCAGGCTCACATGAGAAGCAGTTCCAGATTTCAACTGCTCATCAGA202330.21138740050437096No Hit
TGGCTTGGAGGTCCTGTGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA197390.2062262590103187No Hit
GGGAAGTAGTCCTTGACCAGTCAGCCCAGGGCCGCTGTGCCCCCAGAGGT196900.20571432392285202No Hit
TTCAGCAGGCTCACATCAGAAGCAGTTACAGATTTCAACTGCTCATCAGA196540.20533820834838667No Hit
TGGCTTGAAGCTCCTCTGAGAAGGGCGGGAACAGAGTGACAGTGGGGTTG194300.2029979336628245No Hit
TGGCTTGAAGCTCCTCTGAGCAGGGCGTGAACAGAGTGACCGAGGGGGCA193390.2020471970718149No Hit
TGGCTTGGAGCTCCTCAGAGCAGGGCGTGAACAGAGTGACCGAGGGGGCA188220.19664575951629867No Hit
TGGCTTGGAGGTCCTGTGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA187440.1958308424382904No Hit
GGGAAGTAGTGCTTGTGCAGACAGCCCAGGGCCGCTGTGCTCTCGGAGGT187310.19569502292528904No Hit
TTCAGCAGGCACACAACAGAGGCAGTACCAGATTTCAACTGCTCATCAGA179000.1870130217480473No Hit
GGGAAGTAGTCCTTGTCCAGACAGCCCAGGGCCGCTGTGCTCTCGGAGGT176280.18417125962986466No Hit
TTCAGCAGGCACACAACAGACGCAGTAACAGATTTCAACTGCTCATCAGA175100.18293843635800602No Hit
TGGCTTGGAGCTCCTCTGAGAAGGGCGTGAACAGAGTGACCGAGGGGGCA172950.1806921905660602No Hit
TGGCTTGGAGCTCCTCAGAGTAGGGCGGGAACAGAGTGACAGTGGGGTTG167560.17506090460392626No Hit
TGGCTTGGAGGTCCTGTGAGAAGGGCGGGAACAGAGTGACAGTGGGGTTG162500.1697743912517189No Hit
TGGCTTGAAGCTCCTCTGAGTAGGGCGGGAACAGAGTGACCGAGGGGGCA159320.16645203701060834No Hit
TGGCTTGAAGCTCCTCTGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTG158350.16543861449052116No Hit
TGGCTTGGAGCTCCTCAGAGCAGGGCGTGAACAGAGTGACAGTGGGGTTG157470.1645192208640503No Hit
TGGCTTGGAGGTCCTGTGAGCAGGGCGGGAACAGAGTGACAGTGGGGTTG153310.16017299644800628No Hit
TTCAGCAGGCACACATCAGAAGCAGTTCCAGATTTCAACTGCTCATCAGA151820.1586162958759136No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC151710.15850137167260475No Hit
TTCAGCAGGCACACATCAGAGGCAGTTACAGATTTCAACTGCTCATCAGA150460.1571954148168223No Hit
GGGAAGTAGTCCTTGTCCAGCCAGCCCAGGGCCGCTGTGCTCTCGGAGGT150410.15714317654259102No Hit
GGGAAGTAGTCCTTGACCAGACAGCCCAGGGCCGCTGTGCTCTCGGAGGT149450.1561402016773501No Hit
TGGCTTGGAGCTCCTCTGAGAAGGGCGTGAACAGAGTGACAGTGGGGTTG145340.1518462155355374No Hit
TGGCTTGAAGCTCCTCAGAGGAGGGCGTGAACAGAGTGACCGAGGGGGCA141630.14797013558757507No Hit
TGGCTTGAAGCTCCTGTGAGAAGGGCGTGAACAGAGTGACCGAGGGGGCA135160.1412105029020451No Hit
TTCAGCAGGCACACAACAGACGCAGTACCAGATTTCAACTGCTCATCAGA135150.14120005524719884No Hit
GTCAGCAGGCTCACATCAGAGGCAGTTCCAGATTTCAACTGCTCATCAGA132210.1381284447223985No Hit
TGGCTTGAAGGTCCTGTGAGAAGGGCGTGAACAGAGTGACCGAGGGGGCA132200.13811799706755223No Hit
GCGACGACCACGTTCCCATCTGGCTGGTTGCTGCAGAGGCTCAGCGGGAA129670.13547474039144855No Hit
GTCAGCAGGCTCACAACAGACGCAGTTCCAGATTTCAACTGCTCATCAGA129230.13501504357821315No Hit
CACGCTGCTCGTATCCGACGAGGAATTCTCACAGGAGACGAGGGGGAAAA128990.13476429986190291No Hit
GCGACGACCACGTTCCCATCTTGGGGGGTGCTGTCGAGGCTCAGCGGGAA124430.13000016925200852No Hit
TTCAGCAGGCTCACAAGAGACGCAGTTCCAGATTTCAACTGCTCATCAGA124180.129738977880852No Hit
GGGAAGTAGTCCTTGTCCAGTCAGCCCAGGGCCGCTGTGCCCCCAGAGGT123640.129174804519154No Hit
GGGAAGTAGTGCTTGTCCAGACAGCCCAGGGCCGCTGTGCTCTCGGAGGT122180.1276494469116001No Hit
TCTGATGGCTCAAACACAGCTACCTCGGGTGGGAACACGTTTTTCAGGTC121800.1272524360274422No Hit
TGGCTTGGAGCTCCTCTGAGCAGGGCGTGAACAGAGTGACCGAGGGGGCA121590.12703303527567078No Hit
TTCAGCAGGCTCACATGAGAGGCAGTTACAGATTTCAACTGCTCATCAGA116320.12152712117169195No Hit
GCGACGACCACGTTCCCATCAGGCTGGTTGCTGCAGAGGCTCAGCGGGAA115010.12015847838683193No Hit
TGGCTTGAAGCTCCTGTGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCA113230.11829879582419772No Hit
TTCAGCAGGCACACAACAGAGGCAGTAACAGATTTCAACTGCTCATCAGA110960.11592717817409678No Hit
GCGATGACCACGTTCGCATCAGGCTGGGTGCTGCAGAGGCTCAGCGGGAA110650.11560330087386275No Hit
GTCAGCAGGCTCACATGAGAGGCAGTTCCAGATTTCAACTGCTCATCAGA108020.11285556764929645No Hit
TTCAGCAGGCTCACAACAGAAGCAGTTACAGATTTCAACTGCTCATCAGA106300.11105857101573981No Hit
GCGACGACCAGGTTCGGATCAGGCTGGGTGCTGCAGAGGCTCAGCGGGAA104320.1089899353561804No Hit
GCGATGACCAGGTTCCCATCAGGCTGGGTGCTGCAGAGGCTCAGCGGGAA103070.10768397850039796No Hit
GTCAGCAGGCTCACATGAGACGCAGTTACAGATTTCAACTGCTCATCAGA101290.10582429593776375No Hit
GGGAAGTAGTGCTTGTGCAGCCAGCCCAGGGCCGCTGTGCTCTCGGAGGT101260.10579295297322495No Hit
GGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGG101210.10574071469899365No Hit
GCGATGACCAGGTTCGCATCAGGCTGGGTGCTGCAGAGGCTCAGCGGGAA100550.10505116947914052No Hit
TGGCTTGAAGCTCCTCAGAGAAGGGCGTGAACAGAGTGACAGTGGGGTTG100460.10495714058552418No Hit
GGGAAGTAGTCCTTGTCCAGGCAGCCCAGGGCCGCTGTGCTCTCGGAGGT100360.10485266403706159No Hit
TGGCTTGAAGCTCCTGTGAGCAGGGCGGGAACAGAGTGACAGTGGGGTTG100010.10448699611744251No Hit
TTCAGCAGGCTCACATGAGAAGCAGTTACAGATTTCAACTGCTCATCAGA100010.10448699611744251No Hit
GTCAGCAGGCTCACATCAGACGCAGTTACAGATTTCAACTGCTCATCAGA99080.10351536421674037No Hit
GCGATGACCAGGTTCGGATCTGGCTGGGTGCTGCAGAGGCTCAGCGGGAA98120.10251238935149944No Hit
TGGCTTGAAGCTCCTGTGAGAAGGGCGGGAACAGAGTGACAGTGGGGTTG98080.10247059873211439No Hit
TGGCTTGGAGCTCCTCTGAGCAGGGCGTGAACAGAGTGACAGTGGGGTTG97870.10225119798034293No Hit
GTCAGCAGGCACACAACAGACGCAGTTACAGATTTCAACTGCTCATCAGA97140.10148851917656598No Hit
GTCAGCAGGCTCACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGA96500.10081986926640538No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)