FastQCFastQC Report
Wed 12 Jul 2023
EGAF00008107189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008107189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13749700
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC235325817.114977054044818No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC8934966.498294508243816No Hit
TCTGATGGCTCAAACACAGCTACCTCGGGTGGGAACACGTTTTTCAGGTC6220014.523742336196427No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATCAGGCCTG3877972.820403354255002No Hit
TCTGATGGCTCAAACTCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC3548702.580929038451748No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTG3362292.4453551713855575No Hit
TCTGATGGCTCAAACACAGCCACCTCGGGTGGGAACACGTTTTTCAGGTC3151322.2919190964166494No Hit
TCTGATGGCTCAAACACAGCGACCTCGTGTGGGAACACGTTTTTCAGGTC3062642.2274231437776826No Hit
TCTGATGGCTCAAACACAGCTACCTCGGGTGGGAACACCTTGTTCAGGTC2687101.9542971846658472No Hit
TCTGATGGCTCAAACACAGCTACCTCGTGTGGGAACACGTTTTTCAGGTC1943671.4136090241968915No Hit
TCTGATGGCTCAAACTCAGCTACCTCGGGTGGGAACACGTTTTTCAGGTC1866261.3573096140279426No Hit
TCTGATGGCTCAAACACAGCAACCTCGGGTGGGAACACGTTTTTCAGGTC1808591.3153668807319432No Hit
TCTGATGGCTCAAACTCAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC1737771.263860302406598No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATCAGGCCTG1582701.1510796599198527No Hit
TCTGATGGCTCAAACTCAGCCACCTCGGGTGGGAACACGTTTTTCAGGTC1496451.088351018567678No Hit
TCTGATGGCTCAAACACAGCCACCTCGGGTGGGAACACCTTGTTCAGGTC1482321.078074430714852No Hit
TCTGATGGCTCAAACACAGCGACCTCGTGTGGGAACACCTTGTTCAGGTC1293470.9407259794759159No Hit
TCTGATGGCTCAAACTCAGCAACCTCGGGTGGGAACACGTTTTTCAGGTC1156980.8414583590914712No Hit
TCTGATGGCTCAAACTCAGCTACCTCGGGTGGGAACACCTTGTTCAGGTC1055430.7676022022298669No Hit
TCTGATGGCTCAAACACAGCAACCTCGGGTGGGAACACCTTGTTCAGGTC983050.714961053695717No Hit
TCTGATGGCTCAAACACAGCTACCTCGTGTGGGAACACCTTGTTCAGGTC958000.6967424743812592No Hit
TCTGATGGCTCAAACACAGCAACCTCGTGTGGGAACACGTTTTTCAGGTC955400.6948515240332517No Hit
AGTCTCTCAGCTGGTACACGTCAGGGTCAGGGTTCTGGATATCAGGCCTG953670.6935933147632312No Hit
TCTGATGGCTCAAACTCAGCCACCTCGGGTGGGAACACCTTGTTCAGGTC923370.6715564703229888No Hit
TCTGATGGCTCAAACACAGCCACCTCGTGTGGGAACACGTTTTTCAGGTC891250.6481959606391413No Hit
TCTGATGGCTCAAACTCAGCAACCTCGGGTGGGAACACCTTGTTCAGGTC754580.5487974283075266No Hit
TCTGATGGCTCAAACTCAGCTACCTCGTGTGGGAACACGTTTTTCAGGTC753660.5481283227997702No Hit
TCTGATGGCTCAAACTCAGCGACCTCGTGTGGGAACACGTTTTTCAGGTC727130.5288333563641389No Hit
TCTGATGGCTCAAACTCAGCAACCTCGTGTGGGAACACGTTTTTCAGGTC639440.4650574194346059No Hit
TCTGATGGCTCAAACACAGCAACCTCGTGTGGGAACACCTTGTTCAGGTC586360.4264529407914355No Hit
TCTGATGGCTGAAACTCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC499100.3629897379579191No Hit
TCTGATGGCTCAAACTCAGCCACCTCGTGTGGGAACACGTTTTTCAGGTC494730.35981148679607555No Hit
TCTGATGGCTCAAACACAGCCACCTCGTGTGGGAACACCTTGTTCAGGTC482960.35125130002836424No Hit
TCTGATGGCTCAAACTCAGCTACCTCGTGTGGGAACACCTTGTTCAGGTC472060.34332385433864016No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTGAGTTCC437520.31820330625395465No Hit
TCTGATGGCTCAAACTCAGCAACCTCGTGTGGGAACACCTTGTTCAGGTC430980.31344683884012015No Hit
TCTGATGGCTCAAACTCAGCGACCTCGTGTGGGAACACCTTGTTCAGGTC413630.3008283817101464No Hit
TCTGATGGCTGAAACTCAGCCACCTCGGGTGGGAACACGTTTTTCAGGTC401610.29208637279358823No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATAGGGCTGG371540.27021680473028503No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATTGAGTTCC370700.2696058823101595No Hit
TCTGATGGCTGAAACTCAGCAACCTCGGGTGGGAACACGTTTTTCAGGTC364050.26476941315083236No Hit
TCTGATGGCTGAAACTCAGCTACCTCGGGTGGGAACACGTTTTTCAGGTC358670.2608566005076475No Hit
TCTGATGGCTCAAACTCAGCCACCTCGTGTGGGAACACCTTGTTCAGGTC321390.23374328167160013No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG311630.22664494498061777No Hit
TCTGATGGCTGAAACTCAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC293080.213153741536179No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTGGGTATG281890.20501538215379245No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATACGGATGA274250.19945889728503166No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGA260570.18950958930013018No Hit
TCTGATGGCTGAAACTCAGCCACCTCGGGTGGGAACACCTTGTTCAGGTC256260.1863749754540099No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATAGGACTTG247080.17969846614835233No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTAAGGGTTCTGGATATCAGGCCTG243900.17738568841501995No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTTGGAATG241460.17561110424227439No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGACTTG235780.17148010502047317No Hit
TCTGATGGCTGAAACTCAGCAACCTCGGGTGGGAACACCTTGTTCAGGTC235340.17116009803850266No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTTGGTTTT232970.1694364240674342No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATG231840.1686145879546463No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCC229450.16687636821167007No Hit
TCTGATGGCTGAAACTCAGCTACCTCGGGTGGGAACACCTTGTTCAGGTC224040.16294173691062352No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATAGGGCTGG221600.1611671527378779No Hit
TCTGATGGCTCAAACACAGCGACCTCCGGTGGGAACACGTTTTTCAGGTC215840.1569779704284457No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATTTGGAATG214530.15602522236848804No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTTGCTAAA213670.15539975417645477No Hit
TCTGATGGCTGAAACTCAGCAACCTCGTGTGGGAACACGTTTTTCAGGTC210170.15285424409259837No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATCAGGCCAG203360.14790140875800925No Hit
TCTGATGGCTCAAACACAGCGACCTCCTGTGGGAACACGTTTTTCAGGTC191930.13958850011272975No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATCTGGCTTT189910.1381193771500469No Hit
TCTGATGGCTGAAACTGAGCAACCTCGGGTGGGAACACGTTTTTCAGGTC188510.13710117311650435No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATTTGCTAAA183470.13343563859575117No Hit
AGTCTCTCAGCTGGTACACGACAGGGTAAGGGTTCTGGATATCAGGCCTG175820.1278718808410365No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT171120.12445362444271511No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATCTGGTTTT170530.12402452417143647No Hit
TCTGATGGCTGAAACTGAGCCACCTCGGGTGGGAACACGTTTTTCAGGTC166810.12131901059659483No Hit
TCTGATGGCTGAAACTCAGCTACCTCGTGTGGGAACACGTTTTTCAGGTC166750.12127537328087158No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTAAGGGTTCTGGATATCAGGCCTG160890.11701346211190063No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATTGGGTATG159680.1161334429114817No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATTTGGTTTT156890.11410430773035048No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTTGGATGG151940.11050422918318219No Hit
TCTGATGGCTCAAACACAGCAACCTCCTGTGGGAACACGTTTTTCAGGTC150560.10950057092154737No Hit
TCTGATGGCTCAAACACAGCTACCTCCTGTGGGAACACGTTTTTCAGGTC149450.10869328058066721No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATCGAGCGTG149000.10836600071274281No Hit
TCTGATGGCTGAAACTCAGCCACCTCGTGTGGGAACACGTTTTTCAGGTC146090.1062495909001651No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG145030.10547866498905431No Hit
TCTGATGGCTGAAACTCAGCAACCTCGTGTGGGAACACCTTGTTCAGGTC141010.10255496483559641No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTTTT139990.10181313046830112No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTTGCTTTA139040.10112220630268297No Hit
TCTGATGGCTGAAACTGAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC138640.10083129086452797No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)