FastQCFastQC Report
Wed 12 Jul 2023
EGAF00008107211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008107211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6862136
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC124384218.126163631848744No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC6345459.247047857984745No Hit
TCTGATGGCTCAAACACAGCTACCTCGGGTGGGAACACGTTTTTCAGGTC2918264.252699159561979No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATCAGGCCTG2405493.505453695467417No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTG2071313.01846247290931No Hit
TCTGATGGCTCAAACACAGCTACCTCGGGTGGGAACACCTTGTTCAGGTC1643692.3953037363293292No Hit
TCTGATGGCTCAAACTCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC1547822.2555950508704576No Hit
TCTGATGGCTCAAACACAGCCACCTCGGGTGGGAACACGTTTTTCAGGTC1355411.9752013075811963No Hit
TCTGATGGCTCAAACACAGCGACCTCGTGTGGGAACACGTTTTTCAGGTC1293341.8847484223571205No Hit
TCTGATGGCTCAAACTCAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC926711.3504687170292164No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATCAGGCCTG895291.3046812246216046No Hit
TCTGATGGCTCAAACACAGCCACCTCGGGTGGGAACACCTTGTTCAGGTC807951.1774030709971355No Hit
TCTGATGGCTCAAACTCAGCTACCTCGGGTGGGAACACGTTTTTCAGGTC780681.137663258204151No Hit
TCTGATGGCTCAAACACAGCTACCTCGTGTGGGAACACGTTTTTCAGGTC757601.1040294159136455No Hit
TCTGATGGCTCAAACACAGCAACCTCGGGTGGGAACACGTTTTTCAGGTC708891.0330456872320806No Hit
TCTGATGGCTCAAACACAGCGACCTCGTGTGGGAACACCTTGTTCAGGTC705951.028761306974971No Hit
TCTGATGGCTCAAACTCAGCCACCTCGGGTGGGAACACGTTTTTCAGGTC610800.8901018574974323No Hit
AGTCTCTCAGCTGGTACACGTCAGGGTCAGGGTTCTGGATATCAGGCCTG568830.8289401434189004No Hit
TCTGATGGCTCAAACTCAGCTACCTCGGGTGGGAACACCTTGTTCAGGTC539340.7859651863501395No Hit
TCTGATGGCTCAAACACAGCAACCTCGGGTGGGAACACCTTGTTCAGGTC490470.714748294117167No Hit
TCTGATGGCTCAAACACAGCTACCTCGTGTGGGAACACCTTGTTCAGGTC470590.6857777228548079No Hit
TCTGATGGCTCAAACTCAGCAACCTCGGGTGGGAACACGTTTTTCAGGTC452540.65947395971167No Hit
TCTGATGGCTCAAACTCAGCCACCTCGGGTGGGAACACCTTGTTCAGGTC450220.6560930882162639No Hit
TCTGATGGCTCAAACTCAGCAACCTCGGGTGGGAACACCTTGTTCAGGTC358250.5220677643229454No Hit
TCTGATGGCTCAAACACAGCAACCTCGTGTGGGAACACGTTTTTCAGGTC347920.5070141425352106No Hit
TCTGATGGCTCAAACACAGCCACCTCGTGTGGGAACACGTTTTTCAGGTC325400.4741963726746307No Hit
TCTGATGGCTCAAACTCAGCTACCTCGTGTGGGAACACGTTTTTCAGGTC284720.4149145397293204No Hit
TCTGATGGCTCAAACTCAGCGACCTCGTGTGGGAACACGTTTTTCAGGTC279030.40662266093239774No Hit
TCTGATGGCTCAAACACAGCAACCTCGTGTGGGAACACCTTGTTCAGGTC257080.37463553622370643No Hit
TCTGATGGCTCAAACTCAGCAACCTCGTGTGGGAACACGTTTTTCAGGTC225010.32790081688850237No Hit
TCTGATGGCTCAAACACAGCCACCTCGTGTGGGAACACCTTGTTCAGGTC220780.32173655549817143No Hit
TCTGATGGCTCAAACTCAGCTACCTCGTGTGGGAACACCTTGTTCAGGTC213040.3104572686988425No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTGAGTTCC207030.30169906279910513No Hit
TCTGATGGCTGAAACTCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC196150.28584394130340757No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTGGGTATG193370.28179272459770544No Hit
TCTGATGGCTCAAACTCAGCGACCTCGTGTGGGAACACCTTGTTCAGGTC190810.27806210777518836No Hit
TCTGATGGCTCAAACTCAGCAACCTCGTGTGGGAACACCTTGTTCAGGTC182390.26579187588237835No Hit
TCTGATGGCTCAAACTCAGCCACCTCGTGTGGGAACACGTTTTTCAGGTC180710.2633436585926015No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATCAGGCCAG164480.23969213084672178No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATG156630.22825254410580031No Hit
TCTGATGGCTGAAACTCAGCCACCTCGGGTGGGAACACGTTTTTCAGGTC153670.22393901840476493No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCC152930.22286063697950612No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATACGGATGA151930.22140336478321038No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGA143110.208550224011882No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTTGGAATG140260.20439699825243918No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATAGGGCTGG139150.2027794261145509No Hit
TCTGATGGCTGAAACTCAGCTACCTCGGGTGGGAACACGTTTTTCAGGTC138900.202415108065477No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTG137970.20105984492292195No Hit
TCTGATGGCTCAAACTCAGCCACCTCGTGTGGGAACACCTTGTTCAGGTC137550.20044779060047774No Hit
TCTGATGGCTGAAACTCAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC136840.19941312734110778No Hit
TCTGATGGCTGAAACTCAGCAACCTCGGGTGGGAACACGTTTTTCAGGTC136580.19903423657007088No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATTGAGTTCC135860.19798500058873797No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG135140.19693576460740503No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTTGGTTTA128310.1869825955067052No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATCGAGCGTG127540.18586049591555748No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTAAGGGTTCTGGATATCAGGCCTG123990.18068717961870764No Hit
TCTGATGGCTGAAACTCAGCCACCTCGGGTGGGAACACCTTGTTCAGGTC116740.1701219561955636No Hit
TCTGATGGCTGAAACTCAGCAACCTCGGGTGGGAACACCTTGTTCAGGTC108510.15812860602004972No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG106860.15572410689616178No Hit
TCTGATGGCTGAAACTCAGCTACCTCGGGTGGGAACACCTTGTTCAGGTC104090.1516874629124226No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG100850.14696590099642443No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATTTGGAATG100530.1464995738936098No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATTTGGTTTA99830.14547948335620278No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATTGGGTATG99740.14534832885853619No Hit
TCTGATGGCTCAAACACAGCGACCTCCGGTGGGAACACGTTTTTCAGGTC95600.13931522196587184No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATCAGGCCAG93150.1357449050849473No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTAAGGGTTCTGGATATCAGGCCTG89040.12975551635817187No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA82410.12009380169673116No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCCGGGTTCTGGATATCAGGCCTG79390.11569283966391805No Hit
AGTCTCTCAGCTGGTACACGACAGGGTAAGGGTTCTGGATATCAGGCCTG77730.11327376781806715No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATAGGACTTG76440.11139388668484566No Hit
TCTGATGGCTCAAACACAGCGACCTCCTGTGGGAACACGTTTTTCAGGTC75910.11062153242080892No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTTGGCTGG73900.1076924153062545No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTGGGTTTC73780.10751754264269901No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTTGGTTGC73610.10726980636932874No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGACTTG71900.10477787091366303No Hit
TCTGATGGCTGAAACTCAGCAACCTCGTGTGGGAACACGTTTTTCAGGTC70600.10288341705847859No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTTGGATGG69930.10190704468696045No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTTGGTTTT69040.10061007243225724No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)