FastQCFastQC Report
Wed 8 May 2024
EGAF00008107213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008107213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9714811
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGA8015698.25099942757507No Hit
TTCAGCAGGCACACAACAGACGCAGTTCCAGATTTCAACTGCTCATCAGA4270604.395968176838438No Hit
TGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCA2172012.2357717509892883No Hit
TTCAGCAGGCTCACATCAGACGCAGTTCCAGATTTCAACTGCTCATCAGA2159702.223100377351654No Hit
GCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAGGCTCAGCGGGAA1822991.8765058836450859No Hit
TGGCTTGAAGCTCCTCAGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA1694031.743760120500543No Hit
TTCAGCAGGCTCACAACAGACGCAGTTCCAGATTTCAACTGCTCATCAGA1691291.740939684776163No Hit
TGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCA1591151.6378599645427996No Hit
TTCAGCAGGCTCACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGA1482501.5260204238661976No Hit
TTCAGCAGGCTCACATCAGAGGCAGTTCCAGATTTCAACTGCTCATCAGA1415591.4571462069617207No Hit
TTCAGCAGGCACACAACAGACGCAGTTACAGATTTCAACTGCTCATCAGA1396601.4375987345507804No Hit
TTCAGCAGGCACACAACAGAGGCAGTTACAGATTTCAACTGCTCATCAGA1326451.365389403870029No Hit
TGGCTTGAAGCTCCTCTGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA1279301.3168552635764093No Hit
TGGCTTGGAGCTCCTCAGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA1261471.2985018442458633No Hit
TGGCTTGAAGCTCCTCAGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA1102891.13526655330711No Hit
TGGCTTGAAGCTCCTCTGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA969840.9983107236980729No Hit
TGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTG956660.9847438102501428No Hit
GCGACGACCACGTTCCCATCTGGCTGGGTGCTGCAGAGGCTCAGCGGGAA937220.9647331275924977No Hit
TGGCTTGGAGCTCCTCTGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA878420.9042069886897439No Hit
TTCAGCAGGCACACATCAGACGCAGTTCCAGATTTCAACTGCTCATCAGA866620.8920605866650416No Hit
TGGCTTGGAGCTCCTCAGAGCAGGGCGGGAACAGAGTGACAGTGGGGTTG839450.8640929813251128No Hit
GCGATGACCACGTTCCCATCAGGCTGGGTGCTGCAGAGGCTCAGCGGGAA822180.8463160014126883No Hit
TGGCTTGGAGCTCCTCAGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA815180.83911050868617No Hit
TGGCTTGAAGCTCCTCTGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCA786060.8091356589438539No Hit
TTCAGCAGGCTCACATCAGACGCAGTTACAGATTTCAACTGCTCATCAGA726880.7482183647216606No Hit
TTCAGCAGGCACACATCAGAGGCAGTTCCAGATTTCAACTGCTCATCAGA677620.6975122830490474No Hit
TGGCTTGGAGCTCCTCTGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA673190.6929522355092652No Hit
TTCAGCAGGCTCACATGAGACGCAGTTCCAGATTTCAACTGCTCATCAGA667370.6869613829852171No Hit
GGGAAGTAGTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGT660990.6803940910430475No Hit
TGGCTTGGAGCTCCTCTGAGCAGGGCGGGAACAGAGTGACAGTGGGGTTG636000.6546704820093772No Hit
TTCAGCAGGCACACAACAGAAGCAGTTCCAGATTTCAACTGCTCATCAGA630080.6485766938749503No Hit
TGGCTTGGAGCTCCTCAGAGAAGGGCGGGAACAGAGTGACAGTGGGGTTG589610.6069186523546367No Hit
GGGAAGTAGTCCTTGACCAGCCAGCCCAGGGCCGCTGTGCCCCCAGAGGT579210.596213348875238No Hit
TTCAGCAGGCTCACAACAGACGCAGTTACAGATTTCAACTGCTCATCAGA529530.5450749376390338No Hit
TGGCTTGGAGCTCCTCTGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCA522210.5375400509593032No Hit
TGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTG512270.5273082512876474No Hit
TGGCTTGGAGCTCCTCTGAGAAGGGCGGGAACAGAGTGACAGTGGGGTTG508910.5238496147789184No Hit
GGGAAGTAGTCCTTGTCCAGACAGCCCAGGGCCGCTGTGCCCCCAGAGGT491740.5061755704768729No Hit
GGGAAGTAGTGCTTGTGCAGACAGCCCAGGGCCGCTGTGCCCCCAGAGGT437490.45033300184635605No Hit
TGGCTTGAAGCTCCTCAGAGCAGGGCGGGAACAGAGTGACAGTGGGGTTG434800.44756403392716543No Hit
TTCAGCAGGCTCACATCAGAAGCAGTTCCAGATTTCAACTGCTCATCAGA433380.446102348259786No Hit
TGGCTTGAAGCTCCTGTGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA419150.43145461090287807No Hit
GGGAAGTAGTCCTTGACCAGACAGCCCAGGGCCGCTGTGCCCCCAGAGGT416960.4292003210355816No Hit
TGGCTTGAAGCTCCTGTGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA397370.40903523496236827No Hit
TGGCTTGAAGCTCCTCAGAGAAGGGCGTGAACAGAGTGACCGAGGGGGCA394510.40609127650553367No Hit
GGGAAGTAGTGCTTGTCCAGACAGCCCAGGGCCGCTGTGCCCCCAGAGGT387350.39872108680240925No Hit
GCGACGACCACGTTCCCATCAGGCTGGGTGCTGCAGAGGCTCAGCGGGAA383780.3950462855118849No Hit
GGGAAGTAGTCCTTGTCCAGCCAGCCCAGGGCCGCTGTGCCCCCAGAGGT367630.3784221844357034No Hit
TTCAGCAGGCTCACATCAGAGGCAGTTACAGATTTCAACTGCTCATCAGA366870.37763987379682423No Hit
TTCAGCAGGCTCACATGAGAGGCAGTTCCAGATTTCAACTGCTCATCAGA360220.37079465570663184No Hit
TGGCTTGAAGCTCCTCAGAGTAGGGCGGGAACAGAGTGACCGAGGGGGCA359000.36953884126001013No Hit
TGGCTTGAAGCTCCTCTGAGCAGGGCGGGAACAGAGTGACAGTGGGGTTG347080.3572689165028532No Hit
TGGCTTGGAGCTCCTCTGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTG336660.34654302590137886No Hit
TTCAGCAGGCACACAACAGAAGCAGTTACAGATTTCAACTGCTCATCAGA333720.34351671895624114No Hit
TTCAGCAGGCTCACAACAGAGGCAGTTACAGATTTCAACTGCTCATCAGA320850.33026890590048535No Hit
TTCAGCAGGCACACATCAGACGCAGTTACAGATTTCAACTGCTCATCAGA308980.31805044894851786No Hit
TGGCTTGAAGCTCCTCTGAGAAGGGCGTGAACAGAGTGACCGAGGGGGCA306680.31568292990980473No Hit
TGGCTTGAAGCTCCTCAGAGAAGGGCGGGAACAGAGTGACAGTGGGGTTG305190.3141491893151601No Hit
TGGCTTGAAGGTCCTGTGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA298090.30684076097826296No Hit
TGGCTTGAAGCTCCTCAGAGCAGGGCGTGAACAGAGTGACCGAGGGGGCA292370.3009528440645937No Hit
TGGCTTGGAGCTCCTCAGAGAAGGGCGTGAACAGAGTGACCGAGGGGGCA290510.2990382417115474No Hit
TGGCTTGAAGGTCCTGTGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA289520.298019179168797No Hit
TGGCTTGGAGCTCCTGTGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA282350.2906386959046347No Hit
TTCAGCAGGCTCACAACAGAAGCAGTTCCAGATTTCAACTGCTCATCAGA281780.2900519629254753No Hit
TGGCTTGAAGCTCCTCTGAGAAGGGCGGGAACAGAGTGACAGTGGGGTTG276270.28438021079360165No Hit
TGGCTTGGAGCTCCTGTGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA271640.2796142920330617No Hit
TTCAGCAGGCACACAACAGATGCAGTTCCAGATTTCAACTGCTCATCAGA254150.2616108537778038No Hit
TGGCTTGGAGCTCCTCAGAGTAGGGCGGGAACAGAGTGACCGAGGGGGCA253780.26122999201940217No Hit
GGGAAGTAGTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCTCTCGGAGGT250280.257627245656143No Hit
GGGAAGTAGTGCTTGTGCAGCCAGCCCAGGGCCGCTGTGCCCCCAGAGGT243680.25083349537113997No Hit
TTCAGCAGGCTCACATGAGACGCAGTTACAGATTTCAACTGCTCATCAGA241050.2481262888181767No Hit
GGGAAGTAGTGCTTGTCCAGCCAGCCCAGGGCCGCTGTGCCCCCAGAGGT239920.24696311642089588No Hit
GCGATGACCACGTTCCCATCAGGCTGGTTGCTGCAGAGGCTCAGCGGGAA237960.24494557845747078No Hit
TGGCTTGGAGCTCCTCAGAGAAGGGCGTGAACAGAGTGACAGTGGGGTTG235930.24285598556678048No Hit
TGGCTTGGAGCTCCTGTGAGCAGGGCGGGAACAGAGTGACAGTGGGGTTG227390.23406528444042812No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC226390.23303592833663977No Hit
GCGATGACCACGTTCCCATCTGGCTGGTTGCTGCAGAGGCTCAGCGGGAA221200.22769357015797836No Hit
TGGCTTGGAGCTCCTCAGAGCAGGGCGTGAACAGAGTGACCGAGGGGGCA218830.22525399619200004No Hit
TGGCTTGAAGCTCCTCTGAGCAGGGCGTGAACAGAGTGACCGAGGGGGCA216490.22284530290913535No Hit
TGGCTTGGAGCTCCTGTGAGAAGGGCGGGAACAGAGTGACAGTGGGGTTG215170.22148655285213475No Hit
GTCAGCAGGCACACAACAGACGCAGTTCCAGATTTCAACTGCTCATCAGA214270.22056013235872526No Hit
TGGCTTGGAGCTCCTCTGAGAAGGGCGTGAACAGAGTGACCGAGGGGGCA214130.22041602250419487No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA213940.22022044484447512No Hit
TTCAGCAGGCACACAACAGACGCAGTAACAGATTTCAACTGCTCATCAGA209010.21514571925279866No Hit
GGGAAGTAGTCCTTGACCAGTCAGCCCAGGGCCGCTGTGCCCCCAGAGGT206660.21272673240889606No Hit
GGGAAGTAGTCCTTGACCAGCCAGCCCAGGGCCGCTGTGCTCTCGGAGGT206570.21263409035955513No Hit
GTCAGCAGGCTCACATCAGACGCAGTTCCAGATTTCAACTGCTCATCAGA204790.21080183649481188No Hit
CACGCTGCTCGTATCCGACGCGGAATTCTCACAGGAGACGAGGGGGAAAA200940.2068388154952268No Hit
TGGCTTGGAGGTCCTGTGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA199990.20586092719662794No Hit
GTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGA199130.20497568094736995No Hit
TTCAGCAGGCACACAACAGAGGCAGTACCAGATTTCAACTGCTCATCAGA198450.2042757187967939No Hit
TTCAGCAGGCTCACATCAGAAGCAGTTACAGATTTCAACTGCTCATCAGA197010.20279344600733867No Hit
TGGCTTGGAGGTCCTGTGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA196670.20244346493205065No Hit
TGGCTTGAAGCTCCTCTGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTG193490.19917011252200378No Hit
GGGAAGTAGTCCTTGTCCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGT190630.19622615406516916No Hit
TGGCTTGAAGCTCCTCTGAGTAGGGCGGGAACAGAGTGACCGAGGGGGCA181520.1868487199596575No Hit
TTCAGCAGGCACACAACAGACGCAGTACCAGATTTCAACTGCTCATCAGA180010.18529439224293712No Hit
TTCAGCAGGCACACATCAGAGGCAGTTACAGATTTCAACTGCTCATCAGA176740.18192839778354924No Hit
GTCAGCAGGCTCACATGAGACGCAGTTCCAGATTTCAACTGCTCATCAGA175890.18105344509532917No Hit
TTCAGCAGGCACACATCAGAAGCAGTTCCAGATTTCAACTGCTCATCAGA170780.17579343540497083No Hit
GGGAAGTAGTGCTTGTGCAGACAGCCCAGGGCCGCTGTGCTCTCGGAGGT170600.17560815130628893No Hit
GGGAAGTAGTCCTTGTCCAGACAGCCCAGGGCCGCTGTGCTCTCGGAGGT170050.17504200544920534No Hit
TGGCTTGGAGCTCCTCTGAGAAGGGCGTGAACAGAGTGACAGTGGGGTTG169750.17473319861806885No Hit
TGGCTTGGAGCTCCTCAGAGTAGGGCGGGAACAGAGTGACAGTGGGGTTG169110.1740744107116443No Hit
TTCAGCAGGCTCACATGAGAAGCAGTTCCAGATTTCAACTGCTCATCAGA166820.17171718523396903No Hit
TGGCTTGGAGCTCCTCAGAGCAGGGCGTGAACAGAGTGACAGTGGGGTTG164070.16888645594855112No Hit
TGGCTTGGAGCTCCTCTGAGCAGGGCGTGAACAGAGTGACCGAGGGGGCA149090.15346670151380198No Hit
TGGCTTGGAGGTCCTGTGAGAAGGGCGGGAACAGAGTGACAGTGGGGTTG148840.15320936248785488No Hit
TGGCTTGGAGGTCCTGTGAGCAGGGCGGGAACAGAGTGACAGTGGGGTTG148400.152756445802188No Hit
TGGCTTGAAGCTCCTGTGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCA145300.1495654418804442No Hit
TGGCTTGAAGCTCCTCAGAGGAGGGCGTGAACAGAGTGACCGAGGGGGCA142200.1463744379587004No Hit
TGGCTTGAAGCTCCTGTGAGAAGGGCGTGAACAGAGTGACCGAGGGGGCA139940.14404809316413875No Hit
GGGAAGTAGTCCTTGACCAGACAGCCCAGGGCCGCTGTGCTCTCGGAGGT138500.14256582037468357No Hit
GGGAAGTAGTCCTTGTCCAGCCAGCCCAGGGCCGCTGTGCTCTCGGAGGT133040.1369455360479993No Hit
GTCAGCAGGCTCACAACAGACGCAGTTCCAGATTTCAACTGCTCATCAGA129590.13339425748992956No Hit
GCGATGACCAGGTTCGGATCAGGCTGGGTGCTGCAGAGGCTCAGCGGGAA129230.13302368929256575No Hit
TTCAGCAGGCTCACAACAGAAGCAGTTACAGATTTCAACTGCTCATCAGA127840.13159288430829996No Hit
TTCAGCAGGCTCACAAGAGACGCAGTTCCAGATTTCAACTGCTCATCAGA126820.1305429410824359No Hit
TGGCTTGAAGCTCCTGTGAGCAGGGCGGGAACAGAGTGACAGTGGGGTTG126530.13024442781233728No Hit
TGGCTTGGAGCTCCTCTGAGTAGGGCGGGAACAGAGTGACCGAGGGGGCA122380.1259725999816157No Hit
GGGAAGTAGTCCTTGTCCAGTCAGCCCAGGGCCGCTGTGCCCCCAGAGGT122370.12596230642057782No Hit
TGGCTTGAAGCTCCTCAGAGAAGGGCGTGAACAGAGTGACAGTGGGGTTG122000.12558144466217613No Hit
GTCAGCAGGCTCACATCAGAGGCAGTTCCAGATTTCAACTGCTCATCAGA121730.1253035185141533No Hit
TGGCTTGAAGCTCCTGTGAGAAGGGCGGGAACAGAGTGACAGTGGGGTTG121520.12508735373235774No Hit
TTCAGCAGGCACACAACAGAGGCAGTAACAGATTTCAACTGCTCATCAGA120320.12385212640781175No Hit
TGGCTTGGAGCTCCTCTGAGCAGGGCGTGAACAGAGTGACAGTGGGGTTG115910.1193126659901052No Hit
GGGAAGTAGTGCTTGTCCAGACAGCCCAGGGCCGCTGTGCTCTCGGAGGT113300.11662604655921767No Hit
TGGCTTGAAGGTCCTGTGAGAAGGGCGTGAACAGAGTGACCGAGGGGGCA110320.11355856536992845No Hit
GGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGG106090.10920438905090382No Hit
GCGACGACCACGTTCCCATCTGGCTGGTTGCTGCAGAGGCTCAGCGGGAA103700.1067442279628497No Hit
TGGCTTGGAGCTCCTCAGAGGAGGGCGTGAACAGAGTGACCGAGGGGGCA101890.10488109341499285No Hit
GCGACGACCACGTTCCCATCAGGCTGGTTGCTGCAGAGGCTCAGCGGGAA101630.10461346082800786No Hit
TTCAGCAGGCTCACATGAGAGGCAGTTACAGATTTCAACTGCTCATCAGA101240.10421201194753044No Hit
TTCAGCAGGCTCACATCAGACGCAGTACCAGATTTCAACTGCTCATCAGA98510.10140186978418828No Hit
TTCAGCAGGCTCACATCAGACGCAGTAACAGATTTCAACTGCTCATCAGA98330.10121658568550639No Hit
GGGAAGTAGTGCTTGTGCAGCCAGCCCAGGGCCGCTGTGCTCTCGGAGGT98220.10110335651408967No Hit
TGGCTTGAAGCTCCTGAGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA97730.1005989720232334No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)