FastQCFastQC Report
Wed 12 Jul 2023
EGAF00008107215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008107215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5558990
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGA5409229.730580555100838No Hit
TTCAGCAGGCACACAACAGACGCAGTTCCAGATTTCAACTGCTCATCAGA2551204.589322880595216No Hit
GCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAGGCTCAGCGGGAA1429392.571312414665254No Hit
TGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCA1384432.490434413445608No Hit
TTCAGCAGGCTCACATCAGACGCAGTTCCAGATTTCAACTGCTCATCAGA1089001.9589889530292373No Hit
TGGCTTGAAGCTCCTCAGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA1010091.8170387066715354No Hit
TGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCA983221.768702588060061No Hit
TTCAGCAGGCTCACAACAGACGCAGTTCCAGATTTCAACTGCTCATCAGA936361.6844066997781972No Hit
TTCAGCAGGCTCACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGA864961.5559661017558946No Hit
TTCAGCAGGCACACAACAGAGGCAGTTACAGATTTCAACTGCTCATCAGA815181.4664174607257794No Hit
TTCAGCAGGCACACAACAGACGCAGTTACAGATTTCAACTGCTCATCAGA774741.3936704329383576No Hit
TTCAGCAGGCTCACATCAGAGGCAGTTCCAGATTTCAACTGCTCATCAGA736231.3243952588509784No Hit
TGGCTTGGAGCTCCTCAGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA727101.3079714120730563No Hit
TGGCTTGAAGCTCCTCTGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA702221.2632150804372737No Hit
GCGACGACCACGTTCCCATCTGGCTGGGTGCTGCAGAGGCTCAGCGGGAA700091.2593834491517344No Hit
TGGCTTGAAGCTCCTCAGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA625171.1246107656246906No Hit
TGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTG609611.0966200694730517No Hit
GCGATGACCACGTTCCCATCAGGCTGGGTGCTGCAGAGGCTCAGCGGGAA578111.0399550997573301No Hit
TGGCTTGAAGCTCCTCTGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA519230.9340365785871175No Hit
TGGCTTGGAGCTCCTCAGAGCAGGGCGGGAACAGAGTGACAGTGGGGTTG509050.9157238994853382No Hit
TGGCTTGGAGCTCCTCTGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA464980.8364469085211523No Hit
TGGCTTGAAGCTCCTCTGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCA464360.8353315980061126No Hit
TTCAGCAGGCACACATCAGACGCAGTTCCAGATTTCAACTGCTCATCAGA456790.8217140163950646No Hit
GGGAAGTAGTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGT442470.7959539412735047No Hit
TGGCTTGGAGCTCCTCAGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA440510.7924281209356376No Hit
GGGAAGTAGTCCTTGACCAGCCAGCCCAGGGCCGCTGTGCCCCCAGAGGT378440.6807711472767536No Hit
TTCAGCAGGCACACATCAGAGGCAGTTCCAGATTTCAACTGCTCATCAGA370700.6668477547180333No Hit
TGGCTTGGAGCTCCTCTGAGCAGGGCGGGAACAGAGTGACAGTGGGGTTG358940.6456928326908304No Hit
TTCAGCAGGCACACAACAGAAGCAGTTCCAGATTTCAACTGCTCATCAGA346570.6234405890278629No Hit
TGGCTTGGAGCTCCTCTGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA346520.6233506446314888No Hit
TGGCTTGGAGCTCCTCAGAGAAGGGCGGGAACAGAGTGACAGTGGGGTTG339740.6111541844831525No Hit
TTCAGCAGGCTCACATGAGACGCAGTTCCAGATTTCAACTGCTCATCAGA339270.6103087071572354No Hit
TTCAGCAGGCTCACATCAGACGCAGTTACAGATTTCAACTGCTCATCAGA337290.6067469090608185No Hit
TGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTG311460.5602816338939267No Hit
GGGAAGTAGTCCTTGTCCAGACAGCCCAGGGCCGCTGTGCCCCCAGAGGT308210.5544352481296063No Hit
TGGCTTGGAGCTCCTCTGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCA296930.5341437923075955No Hit
TGGCTTGGAGCTCCTCTGAGAAGGGCGGGAACAGAGTGACAGTGGGGTTG278660.5012781098724768No Hit
TTCAGCAGGCTCACAACAGACGCAGTTACAGATTTCAACTGCTCATCAGA274350.49352490290502415No Hit
GGGAAGTAGTGCTTGTGCAGACAGCCCAGGGCCGCTGTGCCCCCAGAGGT261100.46968963786587126No Hit
GGGAAGTAGTCCTTGACCAGACAGCCCAGGGCCGCTGTGCCCCCAGAGGT259540.4668833726989975No Hit
GCGACGACCACGTTCCCATCAGGCTGGGTGCTGCAGAGGCTCAGCGGGAA254790.45833865504345217No Hit
TGGCTTGAAGCTCCTCAGAGCAGGGCGGGAACAGAGTGACAGTGGGGTTG250710.45099919229932056No Hit
GGGAAGTAGTGCTTGTCCAGACAGCCCAGGGCCGCTGTGCCCCCAGAGGT237590.42739778269074064No Hit
GGGAAGTAGTCCTTGTCCAGCCAGCCCAGGGCCGCTGTGCCCCCAGAGGT230140.41399606763099056No Hit
TGGCTTGAAGCTCCTCAGAGTAGGGCGGGAACAGAGTGACCGAGGGGGCA212990.38314513967465313No Hit
TGGCTTGAAGCTCCTGTGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA210150.3780362979606008No Hit
TTCAGCAGGCTCACATCAGAAGCAGTTCCAGATTTCAACTGCTCATCAGA207400.37308935616002187No Hit
TGGCTTGAAGCTCCTCAGAGAAGGGCGTGAACAGAGTGACCGAGGGGGCA204160.3672609592749762No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA202260.3638430722127581No Hit
CACGCTGCTCGTATCCGACGCGGAATTCTCACAGGAGACGAGGGGGAAAA201920.3632314503174138No Hit
TGGCTTGGAGCTCCTCTGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTG197530.3553343323157624No Hit
TGGCTTGAAGCTCCTGTGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA197150.35465075490331877No Hit
TTCAGCAGGCTCACATGAGAGGCAGTTCCAGATTTCAACTGCTCATCAGA189660.34117708432646937No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC189640.3411411065679197No Hit
TGGCTTGAAGCTCCTCTGAGCAGGGCGGGAACAGAGTGACAGTGGGGTTG187850.3379210971777247No Hit
TTCAGCAGGCTCACATCAGAGGCAGTTACAGATTTCAACTGCTCATCAGA175780.31620851989300214No Hit
TTCAGCAGGCTCACAACAGAGGCAGTTACAGATTTCAACTGCTCATCAGA172510.310326156370132No Hit
TTCAGCAGGCACACAACAGAAGCAGTTACAGATTTCAACTGCTCATCAGA170720.307106146979937No Hit
GGGAAGTAGTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCTCTCGGAGGT166100.2987952847549645No Hit
TGGCTTGAAGCTCCTCAGAGAAGGGCGGGAACAGAGTGACAGTGGGGTTG164510.2959350529502661No Hit
TGGCTTGAAGCTCCTCAGAGCAGGGCGTGAACAGAGTGACCGAGGGGGCA159540.2869945799506745No Hit
GCGATGACCACGTTCCCATCTGGCTGGTTGCTGCAGAGGCTCAGCGGGAA157060.2825333378905161No Hit
TGGCTTGAAGCTCCTCTGAGAAGGGCGTGAACAGAGTGACCGAGGGGGCA153440.27602136359302676No Hit
TTCAGCAGGCACACATCAGACGCAGTTACAGATTTCAACTGCTCATCAGA153210.2756076193697056No Hit
GCGATGACCACGTTCCCATCAGGCTGGTTGCTGCAGAGGCTCAGCGGGAA152260.27389867583859656No Hit
GGGAAGTAGTGCTTGTGCAGCCAGCCCAGGGCCGCTGTGCCCCCAGAGGT148720.26753061257530597No Hit
GGGAAGTAGTGCTTGTCCAGCCAGCCCAGGGCCGCTGTGCCCCCAGAGGT148440.2670269239556107No Hit
TTCAGCAGGCACACAACAGATGCAGTTCCAGATTTCAACTGCTCATCAGA147430.2652100471488526No Hit
TTCAGCAGGCTCACAACAGAAGCAGTTCCAGATTTCAACTGCTCATCAGA146880.2642206587887368No Hit
TGGCTTGGAGCTCCTCAGAGTAGGGCGGGAACAGAGTGACCGAGGGGGCA146310.26319529267007136No Hit
TGGCTTGAAGCTCCTCTGAGAAGGGCGGGAACAGAGTGACAGTGGGGTTG145890.2624397597405284No Hit
TGGCTTGGAGCTCCTCAGAGAAGGGCGTGAACAGAGTGACCGAGGGGGCA142540.2564134851834596No Hit
TGGCTTGAAGGTCCTGTGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA142160.25572990777101595No Hit
TGGCTTGAAGGTCCTGTGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA141450.25445269734250286No Hit
TGGCTTGGAGCTCCTGTGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA136700.24590797968695755No Hit
GGGAAGTAGTCCTTGACCAGCCAGCCCAGGGCCGCTGTGCTCTCGGAGGT133200.23961187194076625No Hit
GGGAAGTAGTCCTTGACCAGTCAGCCCAGGGCCGCTGTGCCCCCAGAGGT131390.23635588479202158No Hit
TGGCTTGGAGCTCCTGTGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA129290.23257822014430676No Hit
TGGCTTGGAGCTCCTCAGAGAAGGGCGTGAACAGAGTGACAGTGGGGTTG124200.22342188059341714No Hit
TGGCTTGGAGCTCCTGTGAGCAGGGCGGGAACAGAGTGACAGTGGGGTTG121850.21919449396383153No Hit
TTCAGCAGGCACACAACAGAGGCAGTACCAGATTTCAACTGCTCATCAGA118140.21252061975286876No Hit
GGGAAGTAGTCCTTGTCCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGT117350.211099498290157No Hit
TGGCTTGGAGCTCCTGTGAGAAGGGCGGGAACAGAGTGACAGTGGGGTTG113440.2040658464936976No Hit
TGGCTTGAAGCTCCTCTGAGCAGGGCGTGAACAGAGTGACCGAGGGGGCA111540.20064795943147948No Hit
TGGCTTGGAGCTCCTCAGAGCAGGGCGTGAACAGAGTGACCGAGGGGGCA111170.19998237089831067No Hit
TGGCTTGAAGCTCCTCTGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTG110320.1984533161599499No Hit
TTCAGCAGGCTCACATGAGACGCAGTTACAGATTTCAACTGCTCATCAGA110300.19841733840140025No Hit
TTCAGCAGGCACACAACAGACGCAGTAACAGATTTCAACTGCTCATCAGA105810.19034033160700056No Hit
TGGCTTGGAGCTCCTCTGAGAAGGGCGTGAACAGAGTGACCGAGGGGGCA102290.18400824610225958No Hit
GGGAAGTAGTGCTTGTGCAGACAGCCCAGGGCCGCTGTGCTCTCGGAGGT100660.18107605878046193No Hit
TGGCTTGGAGCTCCTCAGAGTAGGGCGGGAACAGAGTGACAGTGGGGTTG100450.18069829231569043No Hit
GGGAAGTAGTCCTTGTCCAGACAGCCCAGGGCCGCTGTGCTCTCGGAGGT100060.17999672602397196No Hit
TGGCTTGAAGCTCCTCTGAGTAGGGCGGGAACAGAGTGACCGAGGGGGCA99850.17961895955920051No Hit
TTCAGCAGGCACACAACAGACGCAGTACCAGATTTCAACTGCTCATCAGA96960.17442017344877397No Hit
TTCAGCAGGCACACATCAGAGGCAGTTACAGATTTCAACTGCTCATCAGA94410.1698330092336917No Hit
TGGCTTGGAGGTCCTGTGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA93420.16805211018548333No Hit
TGGCTTGGAGGTCCTGTGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA91410.16443634545124203No Hit
TGGCTTGGAGCTCCTCAGAGCAGGGCGTGAACAGAGTGACAGTGGGGTTG90820.16337500157402693No Hit
TGGCTTGGAGCTCCTCTGAGAAGGGCGTGAACAGAGTGACAGTGGGGTTG90100.162079802266239No Hit
TTCAGCAGGCTCACATCAGAAGCAGTTACAGATTTCAACTGCTCATCAGA86480.1555678279687497No Hit
TTCAGCAGGCACACATCAGAAGCAGTTCCAGATTTCAACTGCTCATCAGA85280.15340916245576983No Hit
GGGAAGTAGTCCTTGACCAGACAGCCCAGGGCCGCTGTGCTCTCGGAGGT85130.15313932926664736No Hit
GGGAAGTAGTCCTTGTCCAGCCAGCCCAGGGCCGCTGTGCTCTCGGAGGT82500.14840825401736646No Hit
TGGCTTGAAGCTCCTCAGAGGAGGGCGTGAACAGAGTGACCGAGGGGGCA81500.14660936608988323No Hit
GTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGA80870.14547606669556878No Hit
GTCAGCAGGCACACAACAGACGCAGTTCCAGATTTCAACTGCTCATCAGA80780.1453141667820953No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC80500.1448104781624No Hit
TTCAGCAGGCTCACATGAGAAGCAGTTCCAGATTTCAACTGCTCATCAGA79970.14385706756083388No Hit
GCGATGACCAGGTTCGGATCAGGCTGGGTGCTGCAGAGGCTCAGCGGGAA78820.14178834644422816No Hit
TGGCTTGGAGGTCCTGTGAGAAGGGCGGGAACAGAGTGACAGTGGGGTTG75120.13513246111254024No Hit
GGGAAGTAGTCCTTGTCCAGTCAGCCCAGGGCCGCTGTGCCCCCAGAGGT74400.1338372618047523No Hit
TGGCTTGGAGCTCCTCTGAGCAGGGCGTGAACAGAGTGACCGAGGGGGCA72500.13041937474253415No Hit
TGGCTTGGAGGTCCTGTGAGCAGGGCGGGAACAGAGTGACAGTGGGGTTG72450.13032943034616No Hit
TGGCTTGAAGCTCCTGTGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCA72340.13013155267413687No Hit
CACGCTGCTCGTATCCGACGAGGAATTCTCACAGGAGACGAGGGGGAAAA71020.12775702060985897No Hit
GTCAGCAGGCTCACATCAGACGCAGTTCCAGATTTCAACTGCTCATCAGA70450.12673165449119353No Hit
GCGACGACCACGTTCCCATCTGGCTGGTTGCTGCAGAGGCTCAGCGGGAA69160.12441108906474019No Hit
TTCAGCAGGCTCACAAGAGACGCAGTTCCAGATTTCAACTGCTCATCAGA69030.12417723363416736No Hit
TTCAGCAGGCACACAACAGAGGCAGTAACAGATTTCAACTGCTCATCAGA67580.12156884613931666No Hit
GCGACGACCACGTTCCCATCTTGGGGGGTGCTGTCGAGGCTCAGCGGGAA67330.12111912415744587No Hit
TGGCTTGAAGCTCCTGTGAGAAGGGCGTGAACAGAGTGACCGAGGGGGCA65760.1182948701112972No Hit
GTCAGCAGGCTCACATGAGACGCAGTTCCAGATTTCAACTGCTCATCAGA65130.11716157071698277No Hit
TGGCTTGGAGCTCCTCTGAGTAGGGCGGGAACAGAGTGACCGAGGGGGCA64640.11628011563251599No Hit
GGGAAGTAGTGCTTGTCCAGACAGCCCAGGGCCGCTGTGCTCTCGGAGGT64340.115740449254271No Hit
GGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63680.11455318322213207No Hit
TGGCTTGAAGCTCCTGTGAGCAGGGCGGGAACAGAGTGACAGTGGGGTTG63470.1141754167573606No Hit
TTCAGCAGGCTCACAACAGAAGCAGTTACAGATTTCAACTGCTCATCAGA62540.1125024509848012No Hit
TGGCTTGAAGCTCCTGTGAGAAGGGCGGGAACAGAGTGACAGTGGGGTTG62170.11183686245163241No Hit
TGGCTTGAAGCTCCTCAGAGAAGGGCGTGAACAGAGTGACAGTGGGGTTG62120.11174691805525824No Hit
TGGCTTGGAGCTCCTCTGAGCAGGGCGTGAACAGAGTGACAGTGGGGTTG62010.11154904038323508No Hit
GCGACGACCACGTTCCCATCAGGCTGGTTGCTGCAGAGGCTCAGCGGGAA61730.11104535176353979No Hit
GCGATGACCACGTTCCCATCTTGGGGGGTGCTGTCGAGGCTCAGCGGGAA57370.10320220039971291No Hit
TGGCTTGGAGCTCCTCAGAGGAGGGCGTGAACAGAGTGACCGAGGGGGCA56510.10165515678207732No Hit
GGGAAGTAGTGCTTGTGCAGCCAGCCCAGGGCCGCTGTGCTCTCGGAGGT55980.10070174618051123No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)