FastQCFastQC Report
Wed 12 Jul 2023
EGAF00008107225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008107225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10508635
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC155681914.814664321293868No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC7651737.281373841607403No Hit
TCTGATGGCTCAAACACAGCTACCTCGGGTGGGAACACGTTTTTCAGGTC4358414.147455877951798No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATCAGGCCTG2712682.581381882613679No Hit
TCTGATGGCTCAAACTCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC2534772.412083015539126No Hit
TCTGATGGCTCAAACACAGCTACCTCGGGTGGGAACACCTTGTTCAGGTC2372062.257248443779806No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTG2315902.2038066789835216No Hit
TCTGATGGCTCAAACACAGCCACCTCGGGTGGGAACACGTTTTTCAGGTC2161442.0568227938262202No Hit
TCTGATGGCTCAAACACAGCGACCTCGTGTGGGAACACGTTTTTCAGGTC2001421.9045480216983464No Hit
TCTGATGGCTCAAACTCAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC1478721.4071475505619901No Hit
TCTGATGGCTCAAACTCAGCTACCTCGGGTGGGAACACGTTTTTCAGGTC1435851.3663525281827755No Hit
TCTGATGGCTCAAACACAGCTACCTCGTGTGGGAACACGTTTTTCAGGTC1347341.2821265559228197No Hit
TCTGATGGCTCAAACACAGCAACCTCGGGTGGGAACACGTTTTTCAGGTC1300691.2377344916823165No Hit
TCTGATGGCTCAAACACAGCCACCTCGGGTGGGAACACCTTGTTCAGGTC1229281.1697808516519985No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATCAGGCCTG1227401.1679918467051142No Hit
TCTGATGGCTCAAACTCAGCCACCTCGGGTGGGAACACGTTTTTCAGGTC1162481.1062140801350508No Hit
TCTGATGGCTCAAACACAGCGACCTCGTGTGGGAACACCTTGTTCAGGTC1048600.997846057075919No Hit
TCTGATGGCTCAAACTCAGCTACCTCGGGTGGGAACACCTTGTTCAGGTC970170.9232121964460654No Hit
TCTGATGGCTCAAACTCAGCAACCTCGGGTGGGAACACGTTTTTCAGGTC950000.9040184572021011No Hit
TCTGATGGCTCAAACACAGCAACCTCGGGTGGGAACACCTTGTTCAGGTC869330.8272530162100025No Hit
TCTGATGGCTCAAACTCAGCCACCTCGGGTGGGAACACCTTGTTCAGGTC827440.7873905602392699No Hit
TCTGATGGCTCAAACACAGCTACCTCGTGTGGGAACACCTTGTTCAGGTC815500.7760284756298035No Hit
TCTGATGGCTCAAACTCAGCAACCTCGGGTGGGAACACCTTGTTCAGGTC724670.6895947951375226No Hit
AGTCTCTCAGCTGGTACACGTCAGGGTCAGGGTTCTGGATATCAGGCCTG721440.6865211323830355No Hit
TCTGATGGCTCAAACACAGCAACCTCGTGTGGGAACACGTTTTTCAGGTC714610.6800217154749403No Hit
TCTGATGGCTCAAACACAGCCACCTCGTGTGGGAACACGTTTTTCAGGTC608970.5794948630340667No Hit
TCTGATGGCTCAAACTCAGCTACCTCGTGTGGGAACACGTTTTTCAGGTC581810.5536494511418467No Hit
TCTGATGGCTCAAACTCAGCGACCTCGTGTGGGAACACGTTTTTCAGGTC530200.5045374589563725No Hit
TCTGATGGCTCAAACTCAGCAACCTCGTGTGGGAACACGTTTTTCAGGTC522260.49698176785091497No Hit
TCTGATGGCTCAAACACAGCAACCTCGTGTGGGAACACCTTGTTCAGGTC519290.49415552067418844No Hit
TCTGATGGCTCAAACTCAGCTACCTCGTGTGGGAACACCTTGTTCAGGTC423690.403182715928377No Hit
TCTGATGGCTCAAACTCAGCAACCTCGTGTGGGAACACCTTGTTCAGGTC412170.39222030263683155No Hit
TCTGATGGCTCAAACACAGCCACCTCGTGTGGGAACACCTTGTTCAGGTC394110.37503443596623154No Hit
TCTGATGGCTCAAACTCAGCCACCTCGTGTGGGAACACGTTTTTCAGGTC376800.3585622680776333No Hit
TCTGATGGCTGAAACTCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC373120.3550603860539452No Hit
TCTGATGGCTCAAACTCAGCGACCTCGTGTGGGAACACCTTGTTCAGGTC347360.3305472118881282No Hit
TCTGATGGCTGAAACTCAGCCACCTCGGGTGGGAACACGTTTTTCAGGTC314510.2992872052364556No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTGAGTTCC310990.29593757895292777No Hit
TCTGATGGCTGAAACTCAGCAACCTCGGGTGGGAACACGTTTTTCAGGTC300860.2862978874040254No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATTGAGTTCC285860.2720239117639922No Hit
TCTGATGGCTGAAACTCAGCTACCTCGGGTGGGAACACGTTTTTCAGGTC284680.27090102568030955No Hit
TCTGATGGCTCAAACTCAGCCACCTCGTGTGGGAACACCTTGTTCAGGTC284500.27072973797262917No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG276000.2626411517766104No Hit
TCTGATGGCTGAAACTCAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC254810.24247678218912352No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATAGGGCTGG254640.24231501046520315No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATCAGGCCAG239390.2278031352311694No Hit
TCTGATGGCTGAAACTCAGCCACCTCGGGTGGGAACACCTTGTTCAGGTC237010.22553833109628416No Hit
TCTGATGGCTGAAACTCAGCAACCTCGGGTGGGAACACCTTGTTCAGGTC235190.22380642205196014No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTGGGTATG229380.2182776354873873No Hit
TCTGATGGCTGAAACTCAGCTACCTCGGGTGGGAACACCTTGTTCAGGTC208720.1986176130391816No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCC179360.17067868471975664No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATG176410.16787146951055013No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTAAGGGTTCTGGATATCAGGCCTG174710.1662537522713464No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTTGGTTTA171710.16339895714333974No Hit
TCTGATGGCTGAAACTCAGCAACCTCGTGTGGGAACACGTTTTTCAGGTC170650.1623902628647774No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTTGGTTTT158620.1509425344014708No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATCAGGCCAG158050.15040012332714953No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTTGGCTGG153520.14608938268385951No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATTGGGTATG153400.14597519087873922No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATTTGGTTTT151720.14437650560705553No Hit
TCTGATGGCTGAAACTGAGCAACCTCGGGTGGGAACACGTTTTTCAGGTC150210.1429395920592922No Hit
TCTGATGGCTCAAACACAGCGACCTCCGGTGGGAACACGTTTTTCAGGTC150160.14289201214049208No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG148230.1410554272748078No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATACGGATGA147470.14033221250904612No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTTGGAATG144570.13757257721863972No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATTTGGTTTA143360.13642114318367704No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATTTGGAATG141710.1348510058632734No Hit
TCTGATGGCTGAAACTCAGCAACCTCGTGTGGGAACACCTTGTTCAGGTC135760.12918899552606025No Hit
TCTGATGGCTGAAACTCAGCTACCTCGTGTGGGAACACGTTTTTCAGGTC131950.1255634057134918No Hit
AGTCTCTCAGCTGGTACACGACAGGGTAAGGGTTCTGGATATCAGGCCTG131090.1247450311101299No Hit
TCTGATGGCTGAAACTGAGCCACCTCGGGTGGGAACACGTTTTTCAGGTC128260.12205200770604364No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATAGGACTTG128210.12200442778724353No Hit
TCTGATGGCTCAAACACAGCGACCTCCTGTGGGAACACGTTTTTCAGGTC127850.12166185237188275No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGACTTG122050.11614258179106993No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATCGAGCGTG119560.1137731018348244No Hit
TCTGATGGCTCAAACACAGCAACCTCCTGTGGGAACACGTTTTTCAGGTC118640.11289763132890238No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATAGGGCTGG115870.11026170382737624No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGA113690.10818721936769143No Hit
TCTGATGGCTGAAACTCAGCCACCTCGTGTGGGAACACGTTTTTCAGGTC113600.10810157551385123No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA113240.10775900009849042No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATTTGGCTGG112660.10720707304040915No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTG111910.10649337425840749No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTAAGGGTTCTGGATATCAGGCCTG110730.10537048817472487No Hit
TCTGATGGCTGAAACTGAGCAACCTCGGGTGGGAACACCTTGTTCAGGTC108880.10361003117912079No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATACGGATGA107270.10207795779375722No Hit
TCTGATGGCTCAAACACAGCTACCTCCTGTGGGAACACGTTTTTCAGGTC106650.10148796680063586No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATCTGGCTTT106220.1010787794989549No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)