FastQCFastQC Report
Wed 12 Jul 2023
EGAF00008107227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008107227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9693833
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC154075315.894156625145078No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC7650577.892203218272896No Hit
TCTGATGGCTCAAACACAGCTACCTCGGGTGGGAACACGTTTTTCAGGTC3978404.104052545572015No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATCAGGCCTG3143223.2424944807693716No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTG2807572.8962434157881614No Hit
TCTGATGGCTCAAACTCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC2311452.384454116343865No Hit
TCTGATGGCTCAAACACAGCTACCTCGGGTGGGAACACCTTGTTCAGGTC2227312.2976566648094723No Hit
TCTGATGGCTCAAACACAGCCACCTCGGGTGGGAACACGTTTTTCAGGTC1934361.9954542233190937No Hit
TCTGATGGCTCAAACACAGCGACCTCGTGTGGGAACACGTTTTTCAGGTC1836221.8942146001483622No Hit
TCTGATGGCTCAAACTCAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC1451301.4971374068441246No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATCAGGCCTG1297121.3380878337805076No Hit
TCTGATGGCTCAAACTCAGCTACCTCGGGTGGGAACACGTTTTTCAGGTC1238171.2772759753546403No Hit
TCTGATGGCTCAAACACAGCCACCTCGGGTGGGAACACCTTGTTCAGGTC1190441.22803848591161No Hit
TCTGATGGCTCAAACACAGCTACCTCGTGTGGGAACACGTTTTTCAGGTC1170551.2075202863511265No Hit
TCTGATGGCTCAAACACAGCAACCTCGGGTGGGAACACGTTTTTCAGGTC1102611.1374344905673535No Hit
TCTGATGGCTCAAACACAGCGACCTCGTGTGGGAACACCTTGTTCAGGTC1008131.0399704636958365No Hit
TCTGATGGCTCAAACTCAGCCACCTCGGGTGGGAACACGTTTTTCAGGTC975711.00652652052083No Hit
TCTGATGGCTCAAACTCAGCTACCTCGGGTGGGAACACCTTGTTCAGGTC893880.9221120273064329No Hit
AGTCTCTCAGCTGGTACACGTCAGGGTCAGGGTTCTGGATATCAGGCCTG808730.8342726762468469No Hit
TCTGATGGCTCAAACACAGCAACCTCGGGTGGGAACACCTTGTTCAGGTC792200.8172205978790845No Hit
TCTGATGGCTCAAACTCAGCCACCTCGGGTGGGAACACCTTGTTCAGGTC757800.7817341190012248No Hit
TCTGATGGCTCAAACTCAGCAACCTCGGGTGGGAACACGTTTTTCAGGTC748950.7726046033596824No Hit
TCTGATGGCTCAAACACAGCTACCTCGTGTGGGAACACCTTGTTCAGGTC747220.7708199635789063No Hit
TCTGATGGCTCAAACTCAGCAACCTCGGGTGGGAACACCTTGTTCAGGTC613930.6333201737640828No Hit
TCTGATGGCTCAAACACAGCAACCTCGTGTGGGAACACGTTTTTCAGGTC572960.5910561900540272No Hit
TCTGATGGCTCAAACACAGCCACCTCGTGTGGGAACACGTTTTTCAGGTC523980.5405292210006093No Hit
TCTGATGGCTCAAACTCAGCTACCTCGTGTGGGAACACGTTTTTCAGGTC484220.49951345355340865No Hit
TCTGATGGCTCAAACTCAGCGACCTCGTGTGGGAACACGTTTTTCAGGTC462800.47741693094981114No Hit
TCTGATGGCTCAAACACAGCAACCTCGTGTGGGAACACCTTGTTCAGGTC455200.46957689491865606No Hit
TCTGATGGCTCAAACTCAGCAACCTCGTGTGGGAACACGTTTTTCAGGTC391380.40374122393071965No Hit
TCTGATGGCTCAAACTCAGCTACCTCGTGTGGGAACACCTTGTTCAGGTC378210.3901552667556786No Hit
TCTGATGGCTCAAACACAGCCACCTCGTGTGGGAACACCTTGTTCAGGTC367200.37879753034738683No Hit
TCTGATGGCTCAAACTCAGCAACCTCGTGTGGGAACACCTTGTTCAGGTC330130.34055672302173967No Hit
TCTGATGGCTCAAACTCAGCGACCTCGTGTGGGAACACCTTGTTCAGGTC326010.336306598225903No Hit
TCTGATGGCTGAAACTCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC319920.3300242535640958No Hit
TCTGATGGCTCAAACTCAGCCACCTCGTGTGGGAACACGTTTTTCAGGTC302780.31234290914646456No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTGGGTATG263230.27154377427380894No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTGAGTTCC249960.25785465872993685No Hit
TCTGATGGCTCAAACTCAGCCACCTCGTGTGGGAACACCTTGTTCAGGTC247340.25515190946656496No Hit
TCTGATGGCTGAAACTCAGCCACCTCGGGTGGGAACACGTTTTTCAGGTC247210.2550178035870847No Hit
TCTGATGGCTGAAACTCAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC234390.24179290070295206No Hit
TCTGATGGCTGAAACTCAGCTACCTCGGGTGGGAACACGTTTTTCAGGTC230410.23768719762347876No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATG224450.2315389588411519No Hit
TCTGATGGCTGAAACTCAGCAACCTCGGGTGGGAACACGTTTTTCAGGTC222510.22953768648583073No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTTGGAATG218280.22517408748427997No Hit
TCTGATGGCTGAAACTCAGCCACCTCGGGTGGGAACACCTTGTTCAGGTC193400.19950828531913023No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTAAGGGTTCTGGATATCAGGCCTG182190.1879442321731765No Hit
TCTGATGGCTGAAACTCAGCAACCTCGGGTGGGAACACCTTGTTCAGGTC181550.18728401861265817No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATCAGGCCAG179540.18521053539915533No Hit
TCTGATGGCTGAAACTCAGCTACCTCGGGTGGGAACACCTTGTTCAGGTC178510.18414800420019614No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTTGGTTTT174100.1795987201347496No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCC171620.1770403925877411No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATACGGATGA170320.17569933379293826No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATTTGGAATG169890.175255752806965No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATTGAGTTCC163130.16828224707399025No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGA157930.16291801189477886No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGACTTG154630.15951378572335628No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTG149960.15469628989894915No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATTGGGTATG147380.15203480398310967No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATAGGACTTG146860.15149838046518854No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG146810.15144680128077306No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATCGAGCGTG146540.15116827368492936No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTGGGTTTC142830.14734109820129973No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATAGGGCTGG141530.1460000394064969No Hit
TCTGATGGCTCAAACACAGCGACCTCCGGTGGGAACACGTTTTTCAGGTC138270.1426370765826067No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTTGGTTGC133450.13766484320495306No Hit
AGTCTCTCAGCTGGTACACGACAGGGTAAGGGTTCTGGATATCAGGCCTG129990.1340955636434009No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT129680.13377577270002486No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTAAGGGTTCTGGATATCAGGCCTG126790.1307944958408093No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTTTC126410.13040249403925155No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTTGGTTTA124590.12852501172652758No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATTTGGTTTT122350.12621426426471344No Hit
TCTGATGGCTGAAACTCAGCAACCTCGTGTGGGAACACGTTTTTCAGGTC122080.12593573666886979No Hit
TCTGATGGCTCAAACACAGCGACCTCCTGTGGGAACACGTTTTTCAGGTC119750.12353214667510776No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG119120.12288224895147255No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG118970.12272751139822606No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATTTGGTTTA116270.1199422354397894No Hit
TCTGATGGCTGAAACTGAGCAACCTCGGGTGGGAACACGTTTTTCAGGTC114090.11769338299927386No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATCAGGCCAG111390.1149081070408372No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCCGGGTTCTGGATATCAGGCCTG107050.11043103383357235No Hit
TCTGATGGCTGAAACTCAGCAACCTCGTGTGGGAACACCTTGTTCAGGTC103160.10641817328604691No Hit
TCTGATGGCTGAAACTCAGCTACCTCGTGTGGGAACACGTTTTTCAGGTC101520.10472637603721872No Hit
TCTGATGGCTGAAACTGAGCCACCTCGGGTGGGAACACGTTTTTCAGGTC100970.10415900500864828No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTGC100520.10369479234890884No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTTGGCTGG99640.10278699870319614No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTTGGATGG98920.10204425844761303No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATTTGGTTGC97700.10078572634787498No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)