FastQCFastQC Report
Wed 12 Jul 2023
EGAF00008107245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008107245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5922523
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGA5161418.714883842578578No Hit
TTCAGCAGGCACACAACAGACGCAGTTCCAGATTTCAACTGCTCATCAGA2205193.7233962620322454No Hit
TGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCA1269382.1433095321031255No Hit
GCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAGGCTCAGCGGGAA1169401.974496342183897No Hit
TTCAGCAGGCTCACATCAGACGCAGTTCCAGATTTCAACTGCTCATCAGA1123371.896776086813002No Hit
TGGCTTGAAGCTCCTCAGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA972421.6419016017329102No Hit
TGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCA929421.569297409229141No Hit
TTCAGCAGGCTCACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGA908571.5340928182127784No Hit
TTCAGCAGGCTCACAACAGACGCAGTTCCAGATTTCAACTGCTCATCAGA869881.4687659296553175No Hit
TTCAGCAGGCACACAACAGAGGCAGTTACAGATTTCAACTGCTCATCAGA775091.3087158969243344No Hit
TTCAGCAGGCTCACATCAGAGGCAGTTCCAGATTTCAACTGCTCATCAGA726411.2265211971316954No Hit
TGGCTTGGAGCTCCTCAGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA713211.2042333985026314No Hit
TGGCTTGAAGCTCCTCTGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA688281.1621398515463763No Hit
TTCAGCAGGCACACAACAGACGCAGTTACAGATTTCAACTGCTCATCAGA680411.1488515958485936No Hit
TGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTG614491.0375476802707224No Hit
TGGCTTGAAGCTCCTCAGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA612041.0334109297676008No Hit
GCGACGACCACGTTCCCATCTGGCTGGGTGCTGCAGAGGCTCAGCGGGAA599831.0127947160357165No Hit
TGGCTTGAAGCTCCTCTGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA512440.8652393582937542No Hit
TGGCTTGGAGCTCCTCAGAGCAGGGCGGGAACAGAGTGACAGTGGGGTTG511510.863669081572161No Hit
TGGCTTGGAGCTCCTCTGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA475400.8026984445649261No Hit
GCGATGACCACGTTCCCATCAGGCTGGGTGCTGCAGAGGCTCAGCGGGAA473560.799591660513602No Hit
TGGCTTGGAGCTCCTCAGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA445450.7521287802512544No Hit
TGGCTTGAAGCTCCTCTGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCA445270.7518248557244944No Hit
GGGAAGTAGTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGT404050.6822261390964628No Hit
TTCAGCAGGCACACATCAGACGCAGTTCCAGATTTCAACTGCTCATCAGA395870.6684144578248156No Hit
TTCAGCAGGCTCACATCAGACGCAGTTACAGATTTCAACTGCTCATCAGA376490.6356919171103261No Hit
TGGCTTGGAGCTCCTCTGAGCAGGGCGGGAACAGAGTGACAGTGGGGTTG360080.607984131087376No Hit
TGGCTTGGAGCTCCTCTGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA353390.5966882695094642No Hit
GGGAAGTAGTCCTTGACCAGCCAGCCCAGGGCCGCTGTGCCCCCAGAGGT344100.5810023869894638No Hit
TGGCTTGGAGCTCCTCAGAGAAGGGCGGGAACAGAGTGACAGTGGGGTTG333780.5635773807885592No Hit
TTCAGCAGGCTCACATGAGACGCAGTTCCAGATTTCAACTGCTCATCAGA328160.554088181675276No Hit
TGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTG325540.5496643913413253No Hit
GGGAAGTAGTGCTTGTGCAGACAGCCCAGGGCCGCTGTGCCCCCAGAGGT324500.54790838296449No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC319310.5391452257762444No Hit
TTCAGCAGGCACACATCAGAGGCAGTTCCAGATTTCAACTGCTCATCAGA309120.5219397206224442No Hit
GGGAAGTAGTCCTTGTCCAGACAGCCCAGGGCCGCTGTGCCCCCAGAGGT308490.5208759847787843No Hit
TGGCTTGGAGCTCCTCTGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCA302360.5105256661730144No Hit
TTCAGCAGGCACACAACAGAAGCAGTTCCAGATTTCAACTGCTCATCAGA297580.5024547815179443No Hit
TGGCTTGGAGCTCCTCTGAGAAGGGCGGGAACAGAGTGACAGTGGGGTTG278510.4702556663773193No Hit
GTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGA274540.46355244209266894No Hit
GGGAAGTAGTGCTTGTCCAGACAGCCCAGGGCCGCTGTGCCCCCAGAGGT267890.4523241192984814No Hit
TTCAGCAGGCTCACAACAGACGCAGTTACAGATTTCAACTGCTCATCAGA266380.44977453021288394No Hit
TGGCTTGAAGCTCCTCAGAGCAGGGCGGGAACAGAGTGACAGTGGGGTTG261450.44145037511884716No Hit
CACGCTGCTCGTATCCGACGCGGAATTCTCACAGGAGACGAGGGGGAAAA261120.44089318015312057No Hit
GGGAAGTAGTCCTTGACCAGACAGCCCAGGGCCGCTGTGCCCCCAGAGGT244120.4121891970702351No Hit
GGGAAGTAGTCCTTGTCCAGCCAGCCCAGGGCCGCTGTGCCCCCAGAGGT238820.4032403082267473No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA232360.39233279465525084No Hit
TTCAGCAGGCTCACATCAGAAGCAGTTCCAGATTTCAACTGCTCATCAGA223220.3769001825742171No Hit
TGGCTTGAAGCTCCTGTGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA219270.370230727681429No Hit
GTCAGCAGGCACACAACAGACGCAGTTCCAGATTTCAACTGCTCATCAGA218870.36955533984418465No Hit
GCGACGACCACGTTCCCATCAGGCTGGGTGCTGCAGAGGCTCAGCGGGAA216480.3655198975166496No Hit
TGGCTTGAAGCTCCTCAGAGAAGGGCGTGAACAGAGTGACCGAGGGGGCA215420.363730119747952No Hit
TGGCTTGGAGCTCCTCTGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTG210600.35559169630915743No Hit
TGGCTTGAAGCTCCTGTGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA208270.35165756215720906No Hit
TGGCTTGAAGCTCCTCAGAGTAGGGCGGGAACAGAGTGACCGAGGGGGCA207090.3496651680373381No Hit
GTCAGCAGGCTCACATCAGACGCAGTTCCAGATTTCAACTGCTCATCAGA206920.34937812820650926No Hit
TGGCTTGAAGCTCCTCTGAGCAGGGCGGGAACAGAGTGACAGTGGGGTTG198230.3347053274423755No Hit
TTCAGCAGGCTCACATCAGAGGCAGTTACAGATTTCAACTGCTCATCAGA185860.31381895857559355No Hit
GGGAAGTAGTGCTTGTGCAGCCAGCCCAGGGCCGCTGTGCCCCCAGAGGT181250.30603511375135223No Hit
TGGCTTGAAGGTCCTGTGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA176640.29825126892711096No Hit
TTCAGCAGGCTCACAACAGAGGCAGTTACAGATTTCAACTGCTCATCAGA175920.29703557082007115No Hit
TGGCTTGAAGCTCCTCAGAGAAGGGCGGGAACAGAGTGACAGTGGGGTTG175700.2966641075095867No Hit
TGGCTTGAAGGTCCTGTGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA173630.29316897545184717No Hit
TTCAGCAGGCTCACATGAGAGGCAGTTCCAGATTTCAACTGCTCATCAGA173010.2921221243041183No Hit
GGGAAGTAGTGCTTGTCCAGCCAGCCCAGGGCCGCTGTGCCCCCAGAGGT169780.2866683675183701No Hit
TGGCTTGAAGCTCCTCAGAGCAGGGCGTGAACAGAGTGACCGAGGGGGCA160540.27106690847802534No Hit
TGGCTTGAAGCTCCTCTGAGAAGGGCGTGAACAGAGTGACCGAGGGGGCA157730.26632230892138364No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC156960.26502218733468824No Hit
TTCAGCAGGCACACAACAGAAGCAGTTACAGATTTCAACTGCTCATCAGA155310.2622362125060553No Hit
TGGCTTGGAGCTCCTCAGAGAAGGGCGTGAACAGAGTGACCGAGGGGGCA152270.2571032649429981No Hit
GGGAAGTAGTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCTCTCGGAGGT152170.256934417983687No Hit
TGGCTTGAAGCTCCTCTGAGAAGGGCGGGAACAGAGTGACAGTGGGGTTG148840.2513118142386277No Hit
TGGCTTGGAGCTCCTGTGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA148670.2510247744077988No Hit
TGGCTTGGAGCTCCTCAGAGTAGGGCGGGAACAGAGTGACCGAGGGGGCA146240.24692179329653935No Hit
TTCAGCAGGCACACATCAGACGCAGTTACAGATTTCAACTGCTCATCAGA144190.24346043063066197No Hit
TGGCTTGGAGCTCCTGTGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA142650.24086018745727117No Hit
GTCAGCAGGCTCACATGAGACGCAGTTCCAGATTTCAACTGCTCATCAGA142560.24070822519389115No Hit
TTCAGCAGGCTCACAACAGAAGCAGTTCCAGATTTCAACTGCTCATCAGA138950.23461284996276083No Hit
GTCAGCAGGCTCACAACAGACGCAGTTCCAGATTTCAACTGCTCATCAGA138360.23361665290282538No Hit
TTCAGCAGGCACACAACAGATGCAGTTCCAGATTTCAACTGCTCATCAGA134040.22632246426058625No Hit
GCGATGACCACGTTCCCATCTGGCTGGTTGCTGCAGAGGCTCAGCGGGAA133100.224735302843062No Hit
GGGAAGTAGTCCTTGTCCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGT127910.21597214565481637No Hit
GGGAAGTAGTCCTTGACCAGCCAGCCCAGGGCCGCTGTGCTCTCGGAGGT127260.21487464041929424No Hit
GCGATGACCACGTTCCCATCAGGCTGGTTGCTGCAGAGGCTCAGCGGGAA127140.21467202406812097No Hit
TGGCTTGGAGCTCCTGTGAGCAGGGCGGGAACAGAGTGACAGTGGGGTTG127040.21450317710880987No Hit
TGGCTTGGAGCTCCTCAGAGAAGGGCGTGAACAGAGTGACAGTGGGGTTG125950.21266274525231899No Hit
GTCAGCAGGCTCACATCAGAGGCAGTTCCAGATTTCAACTGCTCATCAGA123860.20913384380271716No Hit
TGGCTTGAAGCTCCTCTGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTG121060.20440612894200663No Hit
TGGCTTGGAGGTCCTGTGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA120280.20308912265938014No Hit
TGGCTTGGAGCTCCTGTGAGAAGGGCGGGAACAGAGTGACAGTGGGGTTG119130.20114738262730258No Hit
TTCAGCAGGCTCACATGAGACGCAGTTACAGATTTCAACTGCTCATCAGA118930.20080968870868043No Hit
TGGCTTGGAGGTCCTGTGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA118550.20016807026329828No Hit
GGGAAGTAGTGCTTGTGCAGACAGCCCAGGGCCGCTGTGCTCTCGGAGGT116860.19731455665094083No Hit
TGGCTTGGAGCTCCTCAGAGCAGGGCGTGAACAGAGTGACCGAGGGGGCA116480.19667293820555867No Hit
GGGAAGTAGTCCTTGACCAGTCAGCCCAGGGCCGCTGTGCCCCCAGAGGT115070.19429219607927228No Hit
TGGCTTGAAGCTCCTCTGAGCAGGGCGTGAACAGAGTGACCGAGGGGGCA113100.1909659109808438No Hit
TTCAGCAGGCACACAACAGAGGCAGTACCAGATTTCAACTGCTCATCAGA112440.1898515210493906No Hit
TGGCTTGGAGCTCCTCTGAGAAGGGCGTGAACAGAGTGACCGAGGGGGCA110420.18644081247130656No Hit
GTCAGCAGGCTCACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGA108330.18291191102170476No Hit
CACGCTGCTCGTATCCGACGAGGAATTCTCACAGGAGACGAGGGGGAAAA106680.18012593619307177No Hit
TGGCTTGGAGCTCCTCAGAGTAGGGCGGGAACAGAGTGACAGTGGGGTTG102830.17362532825959476No Hit
TTCAGCAGGCACACAACAGACGCAGTAACAGATTTCAACTGCTCATCAGA102210.172578477111866No Hit
GGGAAGTAGTCCTTGTCCAGACAGCCCAGGGCCGCTGTGCTCTCGGAGGT100940.17043412072861516No Hit
TTCAGCAGGCTCACATCAGAAGCAGTTACAGATTTCAACTGCTCATCAGA100690.17001200333033742No Hit
TGGCTTGAAGCTCCTCTGAGTAGGGCGGGAACAGAGTGACCGAGGGGGCA99470.16795207042674212No Hit
TGGCTTGGAGGTCCTGTGAGAAGGGCGGGAACAGAGTGACAGTGGGGTTG96300.16259962181658053No Hit
TGGCTTGGAGGTCCTGTGAGCAGGGCGGGAACAGAGTGACAGTGGGGTTG95720.16162030945257622No Hit
TCTGATGGCTCAAACACAGCTACCTCGGGTGGGAACACGTTTTTCAGGTC95090.16055657360891634No Hit
TGGCTTGGAGCTCCTCAGAGCAGGGCGTGAACAGAGTGACAGTGGGGTTG93090.15717963442269453No Hit
TGGCTTGGAGCTCCTCTGAGAAGGGCGTGAACAGAGTGACAGTGGGGTTG91160.15392088810799046No Hit
TTCAGCAGGCACACAACAGACGCAGTACCAGATTTCAACTGCTCATCAGA85920.1450733074400893No Hit
GGGAAGTAGTCCTTGTCCAGCCAGCCCAGGGCCGCTGTGCTCTCGGAGGT85510.1443810349069138No Hit
GTCAGCAGGCACACAACAGACGCAGTTACAGATTTCAACTGCTCATCAGA84350.14242241017890517No Hit
TTCAGCAGGCTCACATGAGAAGCAGTTCCAGATTTCAACTGCTCATCAGA83890.14164571416607416No Hit
TTCAGCAGGCACACATCAGAGGCAGTTACAGATTTCAACTGCTCATCAGA82080.13858958420254341No Hit
TGGCTTGAAGCTCCTCAGAGGAGGGCGTGAACAGAGTGACCGAGGGGGCA81850.1382012361961279No Hit
GGGAAGTAGTCCTTGACCAGACAGCCCAGGGCCGCTGTGCTCTCGGAGGT79080.13352417542321068No Hit
TTCAGCAGGCACACATCAGAAGCAGTTCCAGATTTCAACTGCTCATCAGA78930.13327090498424404No Hit
TGGCTTGGAGCTCCTCTGAGCAGGGCGTGAACAGAGTGACCGAGGGGGCA78740.13295009576155298No Hit
GTCAGCAGGCTCACATCAGACGCAGTTACAGATTTCAACTGCTCATCAGA77940.13159932008706424No Hit
GGGAAGTAGTCCTTGTCCAGTCAGCCCAGGGCCGCTGTGCCCCCAGAGGT77020.13004592806140222No Hit
GCGATGACCAGGTTCGGATCAGGCTGGGTGCTGCAGAGGCTCAGCGGGAA76590.1293198861363645No Hit
GGGAAGTAGTGCTTGTCCAGACAGCCCAGGGCCGCTGTGCTCTCGGAGGT74110.12513248154544945No Hit
TGGCTTGAAGCTCCTGTGAGAAGGGCGTGAACAGAGTGACCGAGGGGGCA72600.12258289245985199No Hit
TGGCTTGAAGCTCCTGTGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCA72420.12227896793309202No Hit
GTCAGCAGGCTCACATGAGAGGCAGTTCCAGATTTCAACTGCTCATCAGA71320.12042165138067003No Hit
TGGCTTGAAGCTCCTGTGAGCAGGGCGGGAACAGAGTGACAGTGGGGTTG70250.11861498891604136No Hit
TGGCTTGAAGGTCCTGTGAGAAGGGCGTGAACAGAGTGACCGAGGGGGCA66650.11253649838084208No Hit
TGGCTTGGAGCTCCTCTGAGTAGGGCGGGAACAGAGTGACCGAGGGGGCA66380.11208061159070214No Hit
TGGCTTGAAGCTCCTGTGAGAAGGGCGGGAACAGAGTGACAGTGGGGTTG66330.11199618811104659No Hit
TGGCTTGAAGCTCCTCAGAGAAGGGCGTGAACAGAGTGACAGTGGGGTTG66240.11184422584766662No Hit
GGGAAGTAGTGCTTGTGCAGCCAGCCCAGGGCCGCTGTGCTCTCGGAGGT65910.11128703088194002No Hit
GTCAGCAGGCACACATCAGACGCAGTTCCAGATTTCAACTGCTCATCAGA65680.1108986828755245No Hit
GGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGG65510.11061164304469563No Hit
TTCAGCAGGCACACAACAGAGGCAGTAACAGATTTCAACTGCTCATCAGA64690.1092270979783447No Hit
TGGCTTGGAGCTCCTCTGAGCAGGGCGTGAACAGAGTGACAGTGGGGTTG63440.10711651098695607No Hit
TTCAGCAGGCTCACAACAGAAGCAGTTACAGATTTCAACTGCTCATCAGA63190.10669439358867833No Hit
GCGACGACCACGTTCCCATCTGGCTGGTTGCTGCAGAGGCTCAGCGGGAA60800.10265895126114327No Hit
GCGACGACCACGTTCCCATCTTGGGGGGTGCTGTCGAGGCTCAGCGGGAA60040.10137571437037897No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)