FastQCFastQC Report
Wed 12 Jul 2023
EGAF00008107253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008107253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12925686
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC159388012.331105675938591No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC10162807.862484049202495No Hit
TCTGATGGCTCAAACACAGCTACCTCGGGTGGGAACACGTTTTTCAGGTC4650183.5976272361869226No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATCAGGCCTG3217462.489198639050956No Hit
TCTGATGGCTCAAACACAGCTACCTCGGGTGGGAACACCTTGTTCAGGTC3181942.4617184728145185No Hit
TCTGATGGCTCAAACTCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC2801352.16727375243372No Hit
TCTGATGGCTCAAACACAGCCACCTCGGGTGGGAACACGTTTTTCAGGTC2485411.9228457197552222No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTG2420201.8723957861888334No Hit
TCTGATGGCTCAAACACAGCGACCTCGTGTGGGAACACGTTTTTCAGGTC2346141.8150990206631974No Hit
TCTGATGGCTCAAACTCAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC1814491.4037862284446643No Hit
TCTGATGGCTCAAACACAGCCACCTCGGGTGGGAACACCTTGTTCAGGTC1698201.3138180828468216No Hit
TCTGATGGCTCAAACTCAGCTACCTCGGGTGGGAACACGTTTTTCAGGTC1607951.2439958699290699No Hit
TCTGATGGCTCAAACACAGCTACCTCGTGTGGGAACACGTTTTTCAGGTC1596011.234758449184051No Hit
TCTGATGGCTCAAACACAGCGACCTCGTGTGGGAACACCTTGTTCAGGTC1574331.2179856450172162No Hit
TCTGATGGCTCAAACACAGCAACCTCGGGTGGGAACACGTTTTTCAGGTC1538911.190582844113651No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATCAGGCCTG1522451.177848510322779No Hit
TCTGATGGCTCAAACTCAGCCACCTCGGGTGGGAACACGTTTTTCAGGTC1429601.1060147987503333No Hit
TCTGATGGCTCAAACTCAGCAACCTCGGGTGGGAACACGTTTTTCAGGTC1230270.9518024807348715No Hit
TCTGATGGCTCAAACACAGCTACCTCGTGTGGGAACACCTTGTTCAGGTC1159840.8973140767925201No Hit
TCTGATGGCTCAAACTCAGCTACCTCGGGTGGGAACACCTTGTTCAGGTC1153700.8925638453541267No Hit
TCTGATGGCTCAAACACAGCAACCTCGGGTGGGAACACCTTGTTCAGGTC1152000.8912486346952883No Hit
TCTGATGGCTCAAACTCAGCCACCTCGGGTGGGAACACCTTGTTCAGGTC1056680.8175039994008828No Hit
TCTGATGGCTCAAACTCAGCAACCTCGGGTGGGAACACCTTGTTCAGGTC960310.7429470281113126No Hit
TCTGATGGCTCAAACACAGCAACCTCGTGTGGGAACACGTTTTTCAGGTC922670.71382671681797No Hit
AGTCTCTCAGCTGGTACACGTCAGGGTCAGGGTTCTGGATATCAGGCCTG811660.6279434607958139No Hit
TCTGATGGCTCAAACACAGCCACCTCGTGTGGGAACACGTTTTTCAGGTC777100.6012060017549552No Hit
TCTGATGGCTCAAACTCAGCAACCTCGTGTGGGAACACGTTTTTCAGGTC737380.5704764915378573No Hit
TCTGATGGCTCAAACACAGCAACCTCGTGTGGGAACACCTTGTTCAGGTC730530.5651769662360667No Hit
TCTGATGGCTCAAACTCAGCTACCTCGTGTGGGAACACGTTTTTCAGGTC698640.5405051615829133No Hit
TCTGATGGCTCAAACTCAGCGACCTCGTGTGGGAACACGTTTTTCAGGTC631430.4885079213590675No Hit
TCTGATGGCTCAAACTCAGCAACCTCGTGTGGGAACACCTTGTTCAGGTC592420.4583277050053668No Hit
TCTGATGGCTCAAACACAGCCACCTCGTGTGGGAACACCTTGTTCAGGTC571910.4424600752331443No Hit
TCTGATGGCTCAAACTCAGCTACCTCGTGTGGGAACACCTTGTTCAGGTC532820.4122179666131453No Hit
TCTGATGGCTCAAACTCAGCCACCTCGTGTGGGAACACGTTTTTCAGGTC509180.3939288019220024No Hit
TCTGATGGCTGAAACTCAGCCACCTCGGGTGGGAACACGTTTTTCAGGTC467440.3616365119808728No Hit
TCTGATGGCTGAAACTCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC466020.36053792425407827No Hit
TCTGATGGCTGAAACTCAGCAACCTCGGGTGGGAACACGTTTTTCAGGTC459380.3554008661513207No Hit
TCTGATGGCTCAAACTCAGCGACCTCGTGTGGGAACACCTTGTTCAGGTC447770.34641875100478225No Hit
TCTGATGGCTCAAACTCAGCCACCTCGTGTGGGAACACCTTGTTCAGGTC395450.3059412088457046No Hit
TCTGATGGCTGAAACTCAGCAACCTCGGGTGGGAACACCTTGTTCAGGTC365380.28267745325083715No Hit
TCTGATGGCTGAAACTCAGCTACCTCGGGTGGGAACACGTTTTTCAGGTC364520.2820121113881306No Hit
TCTGATGGCTGAAACTCAGCCACCTCGGGTGGGAACACCTTGTTCAGGTC355800.2752658543616176No Hit
TCTGATGGCTGAAACTCAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC350260.27097981492046147No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTGAGTTCC315650.2442036732131664No Hit
TCTGATGGCTGAAACTCAGCTACCTCGGGTGGGAACACCTTGTTCAGGTC283970.21969433575904596No Hit
TCTGATGGCTGAAACTCAGCAACCTCGTGTGGGAACACGTTTTTCAGGTC283340.21920693416194698No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATAGGGCTGG262540.2031149449243932No Hit
TCTGATGGCTGAAACTGAGCAACCTCGGGTGGGAACACGTTTTTCAGGTC244070.18882556794277688No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATTGAGTTCC242900.1879203935481645No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTGGGTATG239560.18533639143021113No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTAAGGGTTCTGGATATCAGGCCTG238750.18470973223394102No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATAGGACTTG228380.17668694721502595No Hit
TCTGATGGCTGAAACTCAGCAACCTCGTGTGGGAACACCTTGTTCAGGTC227020.17563477868795513No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG219310.16966991152345803No Hit
TCTGATGGCTGAAACTGAGCCACCTCGGGTGGGAACACGTTTTTCAGGTC207280.16036286197885358No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTTGGTTTA200990.155496582541151No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGACTTG189990.146986395925137No Hit
AGTCTCTCAGCTGGTACACGACAGGGTAAGGGTTCTGGATATCAGGCCTG185930.14384536341049906No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCC182090.14087453462818142No Hit
TCTGATGGCTGAAACTCAGCCACCTCGTGTGGGAACACGTTTTTCAGGTC179980.13924212610456418No Hit
TCTGATGGCTGAAACTCAGCTACCTCGTGTGGGAACACGTTTTTCAGGTC179840.1391338146385422No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTTGGAATG179280.1387005687744542No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATACGGATGA178530.1381203287779078No Hit
TCTGATGGCTGAAACTGAGCAACCTCGGGTGGGAACACCTTGTTCAGGTC177260.13713778905042254No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATTTGGAATG171270.13250360561133853No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATTTGGTTTA171030.1323179288124437No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATG169260.13094856242059416No Hit
CGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATCAGGCCTG166790.12903763869863463No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATCAGGCCAG160460.12414041312778293No Hit
TCTGATGGCTCAAACACAGCGACCTCCGGTGGGAACACGTTTTTCAGGTC157610.12193550114090657No Hit
TCTGATGGCTCAAACACAGCAACCTCCTGTGGGAACACGTTTTTCAGGTC155860.1205816078156316No Hit
TCTGATGGCTGAAACTGAGCAACCTCGTGTGGGAACACGTTTTTCAGGTC153690.11890278009229066No Hit
TCTGATGGCTCAAACACAGCGACCTCCTGTGGGAACACGTTTTTCAGGTC152780.11819875556314768No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATAGGGCTGG149340.11553738811232146No Hit
TCTGATGGCTGAAACTCAGCTACCTCGTGTGGGAACACCTTGTTCAGGTC147180.1138662969222678No Hit
TCTGATGGCTGAAACTGAGCCACCTCGGGTGGGAACACCTTGTTCAGGTC145690.11271355346246226No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGA145640.11267487079602584No Hit
TCTGATGGCTGAAACTGAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC143750.11121266600472887No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATTGGGTATG143690.11116624680500517No Hit
TCTGATGGCTGAAACTCAGCCACCTCGTGTGGGAACACCTTGTTCAGGTC140150.1084275140213061No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTAAGGGTTCTGGATATCAGGCCTG138900.10746044736039541No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTGGGTTTC137540.1064082788333246No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATCGAGCGTG135630.10493060097545306No Hit
TCTGATGGCTGAAACTCAGCGACCTCGTGTGGGAACACGTTTTTCAGGTC135600.1049073913755912No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTTGGCTGG134660.10418015724658637No Hit
TCTGATGGCTGAAACTGAGCTACCTCGGGTGGGAACACGTTTTTCAGGTC130150.10069098073402062No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)