FastQCFastQC Report
Wed 12 Jul 2023
EGAF00008107261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008107261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15192318
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGA178822711.770600115137137No Hit
TTCAGCAGGCACACAACAGACGCAGTTCCAGATTTCAACTGCTCATCAGA6621894.358709447761691No Hit
TGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCA3756612.4727036387732273No Hit
GCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAGGCTCAGCGGGAA3445132.2676789677519915No Hit
TGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCA2781461.8308331881941913No Hit
TTCAGCAGGCACACAACAGAGGCAGTTACAGATTTCAACTGCTCATCAGA2669781.7573223519939487No Hit
TGGCTTGAAGCTCCTCAGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA2656101.74831780114134No Hit
TTCAGCAGGCTCACATCAGACGCAGTTCCAGATTTCAACTGCTCATCAGA2446891.610610046472171No Hit
TTCAGCAGGCTCACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGA2269191.493643037224471No Hit
TTCAGCAGGCTCACAACAGACGCAGTTCCAGATTTCAACTGCTCATCAGA2019291.3291520095880036No Hit
TGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTG2018261.328474035364452No Hit
TGGCTTGGAGCTCCTCAGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA1994961.313137336909351No Hit
TTCAGCAGGCACACAACAGACGCAGTTACAGATTTCAACTGCTCATCAGA1945991.280903941057579No Hit
GCGACGACCACGTTCCCATCTGGCTGGGTGCTGCAGAGGCTCAGCGGGAA1791831.1794316048413416No Hit
TGGCTTGAAGCTCCTCTGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA1692611.1141222820638694No Hit
TTCAGCAGGCTCACATCAGAGGCAGTTCCAGATTTCAACTGCTCATCAGA1682181.1072569702661568No Hit
TGGCTTGAAGCTCCTCAGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA1615361.0632742152974943No Hit
TGGCTTGGAGCTCCTCAGAGCAGGGCGGGAACAGAGTGACAGTGGGGTTG1517730.9990114740884176No Hit
GGGAAGTAGTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGT1304290.8585194175108761No Hit
TGGCTTGAAGCTCCTCTGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCA1286710.8469477797923924No Hit
GCGATGACCACGTTCCCATCAGGCTGGGTGCTGCAGAGGCTCAGCGGGAA1242100.8175842554111887No Hit
TGGCTTGAAGCTCCTCTGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA1240120.816280965156206No Hit
TGGCTTGGAGCTCCTCAGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA1207240.7946384481946731No Hit
TGGCTTGGAGCTCCTCTGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA1175790.7739371964172945No Hit
TGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTG1121700.7383336762698095No Hit
GGGAAGTAGTCCTTGACCAGCCAGCCCAGGGCCGCTGTGCCCCCAGAGGT1097230.7222268517549462No Hit
TTCAGCAGGCACACATCAGACGCAGTTCCAGATTTCAACTGCTCATCAGA1031770.6791392860523324No Hit
TGGCTTGGAGCTCCTCAGAGAAGGGCGGGAACAGAGTGACAGTGGGGTTG967760.6370061500819033No Hit
TGGCTTGGAGCTCCTCTGAGCAGGGCGGGAACAGAGTGACAGTGGGGTTG922760.607385917014112No Hit
TTCAGCAGGCACACATCAGAGGCAGTTCCAGATTTCAACTGCTCATCAGA884100.5819388456718718No Hit
TTCAGCAGGCACACAACAGAAGCAGTTCCAGATTTCAACTGCTCATCAGA866100.5700907524447553No Hit
TGGCTTGGAGCTCCTCTGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA866050.5700578410746799No Hit
TGGCTTGGAGCTCCTCTGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCA862340.5676158174150909No Hit
TTCAGCAGGCTCACATGAGACGCAGTTCCAGATTTCAACTGCTCATCAGA850490.5598158227072393No Hit
TTCAGCAGGCTCACATCAGACGCAGTTACAGATTTCAACTGCTCATCAGA836030.5502978544814556No Hit
TGGCTTGAAGCTCCTCAGAGCAGGGCGGGAACAGAGTGACAGTGGGGTTG824650.5428072266523121No Hit
CACGCTGCTCGTATCCGACGCGGAATTCTCACAGGAGACGAGGGGGAAAA767530.5052092774782624No Hit
GGGAAGTAGTCCTTGTCCAGACAGCCCAGGGCCGCTGTGCCCCCAGAGGT739700.4868908088943373No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA733710.4829480267593135No Hit
GGGAAGTAGTCCTTGACCAGACAGCCCAGGGCCGCTGTGCCCCCAGAGGT733600.4828756217451478No Hit
TGGCTTGGAGCTCCTCTGAGAAGGGCGGGAACAGAGTGACAGTGGGGTTG708780.4665384176397572No Hit
TGGCTTGGAGCTCCTCTGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTG632810.41653288194731053No Hit
TTCAGCAGGCTCACAACAGACGCAGTTACAGATTTCAACTGCTCATCAGA622710.40988478519209515No Hit
TGGCTTGAAGCTCCTCAGAGTAGGGCGGGAACAGAGTGACCGAGGGGGCA604500.3978984642106623No Hit
GGGAAGTAGTGCTTGTGCAGACAGCCCAGGGCCGCTGTGCCCCCAGAGGT593610.39073036780825676No Hit
GGGAAGTAGTCCTTGTCCAGCCAGCCCAGGGCCGCTGTGCCCCCAGAGGT593410.39059872232795545No Hit
GCGACGACCACGTTCCCATCAGGCTGGGTGCTGCAGAGGCTCAGCGGGAA581690.38288429718229966No Hit
TGGCTTGAAGCTCCTCTGAGCAGGGCGGGAACAGAGTGACAGTGGGGTTG542120.3568382389046886No Hit
TGGCTTGAAGCTCCTCAGAGAAGGGCGTGAACAGAGTGACCGAGGGGGCA525060.34560887943498814No Hit
TGGCTTGAAGCTCCTCAGAGAAGGGCGGGAACAGAGTGACAGTGGGGTTG518760.34146204680549735No Hit
GGGAAGTAGTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCTCTCGGAGGT511610.3367557208847261No Hit
TTCAGCAGGCTCACATCAGAAGCAGTTCCAGATTTCAACTGCTCATCAGA508900.33497192462664355No Hit
TGGCTTGAAGCTCCTGTGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA502200.3305618010365502No Hit
GGGAAGTAGTGCTTGTCCAGACAGCCCAGGGCCGCTGTGCCCCCAGAGGT501350.3300023077452697No Hit
TGGCTTGAAGCTCCTGTGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA485680.31968788436366324No Hit
TTCAGCAGGCTCACATGAGAGGCAGTTCCAGATTTCAACTGCTCATCAGA473000.3113415609125612No Hit
TTCAGCAGGCACACAACAGATGCAGTTCCAGATTTCAACTGCTCATCAGA453900.2987694175437876No Hit
TTCAGCAGGCTCACAACAGAGGCAGTTACAGATTTCAACTGCTCATCAGA445830.2934575224136304No Hit
TTCAGCAGGCACACAACAGAAGCAGTTACAGATTTCAACTGCTCATCAGA440480.2899360058155707No Hit
TGGCTTGGAGCTCCTCAGAGTAGGGCGGGAACAGAGTGACCGAGGGGGCA436950.2876124630882529No Hit
TTCAGCAGGCTCACATCAGAGGCAGTTACAGATTTCAACTGCTCATCAGA427560.2814317077881071No Hit
GGGAAGTAGTCCTTGACCAGCCAGCCCAGGGCCGCTGTGCTCTCGGAGGT410730.2703537406207532No Hit
GGGAAGTAGTCCTTGACCAGTCAGCCCAGGGCCGCTGTGCCCCCAGAGGT406860.2678064005769231No Hit
TGGCTTGAAGCTCCTCTGAGAAGGGCGGGAACAGAGTGACAGTGGGGTTG403000.2652656428071082No Hit
TGGCTTGAAGCTCCTCAGAGCAGGGCGTGAACAGAGTGACCGAGGGGGCA402680.26505501003862614No Hit
TGGCTTGGAGCTCCTCAGAGAAGGGCGTGAACAGAGTGACCGAGGGGGCA387440.2550236244396675No Hit
TGGCTTGAAGCTCCTCTGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTG382220.2515876774038037No Hit
GCGATGACCACGTTCCCATCTGGCTGGTTGCTGCAGAGGCTCAGCGGGAA380510.2504621085472276No Hit
TGGCTTGAAGCTCCTCTGAGAAGGGCGTGAACAGAGTGACCGAGGGGGCA374140.2462691999996314No Hit
TTCAGCAGGCACACATCAGACGCAGTTACAGATTTCAACTGCTCATCAGA371120.24428135324708186No Hit
TTCAGCAGGCACACAACAGAGGCAGTACCAGATTTCAACTGCTCATCAGA367190.24169451955916144No Hit
GGGAAGTAGTCCTTGTCCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGT364030.23961452097040095No Hit
TGGCTTGAAGGTCCTGTGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA351840.23159072894603708No Hit
TGGCTTGGAGCTCCTGTGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA345220.22723326354806422No Hit
TGGCTTGGAGCTCCTCAGAGAAGGGCGTGAACAGAGTGACAGTGGGGTTG340480.22411326566492357No Hit
TGGCTTGGAGCTCCTCAGAGTAGGGCGGGAACAGAGTGACAGTGGGGTTG339440.22342870916735683No Hit
TGGCTTGAAGGTCCTGTGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA333350.2194201042921824No Hit
TGGCTTGGAGCTCCTGTGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA333280.21937402837407696No Hit
TTCAGCAGGCTCACAACAGAAGCAGTTCCAGATTTCAACTGCTCATCAGA330700.21767580167819026No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC329020.21656997964365937No Hit
GGGAAGTAGTGCTTGTGCAGCCAGCCCAGGGCCGCTGTGCCCCCAGAGGT327110.21531276530678203No Hit
GGGAAGTAGTGCTTGTCCAGCCAGCCCAGGGCCGCTGTGCCCCCAGAGGT319100.2100403638207152No Hit
GCGATGACCACGTTCCCATCAGGCTGGTTGCTGCAGAGGCTCAGCGGGAA316860.20856593444134067No Hit
GTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGA311140.20480087370472366No Hit
TTCAGCAGGCTCACATGAGACGCAGTTACAGATTTCAACTGCTCATCAGA306950.20204290089241156No Hit
TGGCTTGGAGCTCCTCAGAGCAGGGCGTGAACAGAGTGACCGAGGGGGCA302670.19922568761396386No Hit
TGGCTTGGAGCTCCTGTGAGCAGGGCGGGAACAGAGTGACAGTGGGGTTG299140.196902144886646No Hit
CACGCTGCTCGTATCCGACGAGGAATTCTCACAGGAGACGAGGGGGAAAA293200.19299227412169756No Hit
TGGCTTGGAGCTCCTGTGAGAAGGGCGGGAACAGAGTGACAGTGGGGTTG291530.19189303436118174No Hit
TGGCTTGAAGCTCCTCTGAGTAGGGCGGGAACAGAGTGACCGAGGGGGCA271570.17875481542711258No Hit
TGGCTTGAAGCTCCTCTGAGCAGGGCGTGAACAGAGTGACCGAGGGGGCA267750.17624038675335785No Hit
TTCAGCAGGCACACAACAGACGCAGTAACAGATTTCAACTGCTCATCAGA264450.1740682363283865No Hit
TGGCTTGGAGCTCCTCTGAGAAGGGCGTGAACAGAGTGACCGAGGGGGCA260440.17142874444834555No Hit
GGGAAGTAGTCCTTGACCAGACAGCCCAGGGCCGCTGTGCTCTCGGAGGT255050.16788089875422565No Hit
TGGCTTGGAGCTCCTCAGAGCAGGGCGTGAACAGAGTGACAGTGGGGTTG253550.16689355765196595No Hit
GGGAAGTAGTCCTTGTCCAGACAGCCCAGGGCCGCTGTGCTCTCGGAGGT252790.16639330482682102No Hit
GTCAGCAGGCACACAACAGACGCAGTTCCAGATTTCAACTGCTCATCAGA252330.16609052022212806No Hit
TGGCTTGGAGGTCCTGTGAGAAGGGCGGGAACAGAGTGACCGAGGGGGCA241910.15923179069843063No Hit
TGGCTTGAAGCTCCTCAGAGGAGGGCGTGAACAGAGTGACCGAGGGGGCA234350.1542555915430417No Hit
TTCAGCAGGCACACAACAGACGCAGTACCAGATTTCAACTGCTCATCAGA232920.15331432635888742No Hit
TTCAGCAGGCTCACATCAGAAGCAGTTACAGATTTCAACTGCTCATCAGA232020.1527219216975316No Hit
TGGCTTGGAGGTCCTGTGAGCAGGGCGGGAACAGAGTGACCGAGGGGGCA229890.15131989733232284No Hit
TTCAGCAGGCACACATCAGAGGCAGTTACAGATTTCAACTGCTCATCAGA228970.15071432812293686No Hit
TGGCTTGGAGCTCCTCTGAGAAGGGCGTGAACAGAGTGACAGTGGGGTTG228170.1501877462017317No Hit
GGGAAGTAGTCCTTGTCCAGCCAGCCCAGGGCCGCTGTGCTCTCGGAGGT223410.14705458377056088No Hit
GGGAAGTAGTCCTTGTCCAGTCAGCCCAGGGCCGCTGTGCCCCCAGAGGT221700.14592901491398483No Hit
TTCAGCAGGCTCACATGAGAAGCAGTTCCAGATTTCAACTGCTCATCAGA217760.1433355989520493No Hit
GTCAGCAGGCTCACATCAGACGCAGTTCCAGATTTCAACTGCTCATCAGA215280.14170319499631326No Hit
GGGAAGTAGTGCTTGTGCAGACAGCCCAGGGCCGCTGTGCTCTCGGAGGT207160.1363583884960807No Hit
TTCAGCAGGCACACATCAGAAGCAGTTCCAGATTTCAACTGCTCATCAGA206830.1361411734535836No Hit
GTCAGCAGGCTCACATGAGACGCAGTTCCAGATTTCAACTGCTCATCAGA202160.13306725148854837No Hit
TGGCTTGGAGGTCCTGTGAGAAGGGCGGGAACAGAGTGACAGTGGGGTTG200160.1317507966855354No Hit
TTCAGCAGGCACACAACAGAGGCAGTAACAGATTTCAACTGCTCATCAGA199740.1314743411769027No Hit
TGGCTTGGAGGTCCTGTGAGCAGGGCGGGAACAGAGTGACAGTGGGGTTG195880.12893358340708772No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC192760.12687991391438752No Hit
TGGCTTGAAGCTCCTCAGAGTAGGGCGGGAACAGAGTGACAGTGGGGTTG191730.12620193969083585No Hit
TGGCTTGGAGCTCCTCTGAGCAGGGCGTGAACAGAGTGACCGAGGGGGCA186080.12248295487232429No Hit
TGGCTTGAAGCTCCTCAGAGAAGGGCGTGAACAGAGTGACAGTGGGGTTG183080.12050827266780488No Hit
TGGCTTGAAGCTCCTGTGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCA182230.11994877937652439No Hit
TGGCTTGGAGCTCCTCTGAGTAGGGCGGGAACAGAGTGACCGAGGGGGCA181510.11947485564743972No Hit
GCGACGACCACGTTCCCATCTGGCTGGTTGCTGCAGAGGCTCAGCGGGAA177810.11703941426186577No Hit
GCGACGACCACGTTCCCATCTTGGGGGGTGCTGTCGAGGCTCAGCGGGAA175960.1158216935690788No Hit
TGGCTTGAAGCTCCTGTGAGAAGGGCGTGAACAGAGTGACCGAGGGGGCA173060.11391283410471002No Hit
TGGCTTGAAGCTCCTGTGAGCAGGGCGGGAACAGAGTGACAGTGGGGTTG170730.11237916425919994No Hit
TGGCTTGGAGCTCCTCAGAGGAGGGCGTGAACAGAGTGACCGAGGGGGCA168820.11112194992232258No Hit
TGGCTTGAAGCTCCTGTGAGAAGGGCGGGAACAGAGTGACAGTGGGGTTG165700.1090682804296224No Hit
TTCAGCAGGCTCACAAGAGACGCAGTTCCAGATTTCAACTGCTCATCAGA161940.10659334539995806No Hit
TGGCTTGGAGCTCCTCTGAGCAGGGCGTGAACAGAGTGACAGTGGGGTTG159710.10512549829459862No Hit
GCGATGACCACGTTCCCATCTTGGGGGGTGCTGTCGAGGCTCAGCGGGAA156840.10323638565227504No Hit
GGGAAGTAGTCCTTGACCAGTCAGCCCAGGGCCGCTGTGCTCTCGGAGGT156780.10319689200818467No Hit
GGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGG155310.10222929772797015No Hit
GGGAAGTAGTCCTTGTCCAGGCAGCCCAGGGCCGCTGTGCTCTCGGAGGT152770.10055740012814371No Hit
TTCAGCAGGCACACAACAGATGCAGTTACAGATTTCAACTGCTCATCAGA151970.10003081820693853No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)