FastQCFastQC Report
Wed 12 Jul 2023
EGAF00008107271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008107271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16105698
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC241172314.974346346243422No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC15471689.606339321648774No Hit
TCTGATGGCTCAAACACAGCTACCTCGGGTGGGAACACGTTTTTCAGGTC6600324.098127259060737No Hit
TCTGATGGCTCAAACACAGCTACCTCGGGTGGGAACACCTTGTTCAGGTC4556892.829365110409993No Hit
TCTGATGGCTCAAACTCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC3714122.306090676728199No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATCAGGCCTG3201601.9878678961942535No Hit
TCTGATGGCTCAAACACAGCGACCTCGTGTGGGAACACGTTTTTCAGGTC3024971.8781986350420825No Hit
TCTGATGGCTCAAACACAGCCACCTCGGGTGGGAACACGTTTTTCAGGTC3012491.87044982465212No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTG2657711.6501675369797697No Hit
TCTGATGGCTCAAACTCAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC2478701.5390205379487434No Hit
TCTGATGGCTCAAACACAGCCACCTCGGGTGGGAACACCTTGTTCAGGTC2064641.2819314009240705No Hit
TCTGATGGCTCAAACTCAGCTACCTCGGGTGGGAACACGTTTTTCAGGTC2050101.2729035401011493No Hit
TCTGATGGCTCAAACACAGCGACCTCGTGTGGGAACACCTTGTTCAGGTC2037441.2650429680228699No Hit
TCTGATGGCTCAAACACAGCTACCTCGTGTGGGAACACGTTTTTCAGGTC2010941.248589163909568No Hit
TCTGATGGCTCAAACACAGCAACCTCGGGTGGGAACACGTTTTTCAGGTC1820471.1303266707223742No Hit
TCTGATGGCTCAAACTCAGCCACCTCGGGTGGGAACACGTTTTTCAGGTC1573720.9771200229881375No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATCAGGCCTG1541860.9573382041560694No Hit
TCTGATGGCTCAAACTCAGCTACCTCGGGTGGGAACACCTTGTTCAGGTC1492430.926647202747748No Hit
TCTGATGGCTCAAACACAGCTACCTCGTGTGGGAACACCTTGTTCAGGTC1472810.9144651787212204No Hit
TCTGATGGCTCAAACACAGCAACCTCGGGTGGGAACACCTTGTTCAGGTC1354070.8407397183282588No Hit
TCTGATGGCTCAAACTCAGCAACCTCGGGTGGGAACACGTTTTTCAGGTC1300570.8075216609674415No Hit
TCTGATGGCTCAAACTCAGCCACCTCGGGTGGGAACACCTTGTTCAGGTC1155560.717485203062916No Hit
TCTGATGGCTCAAACTCAGCAACCTCGGGTGGGAACACCTTGTTCAGGTC1002770.6226181566300324No Hit
TCTGATGGCTCAAACACAGCAACCTCGTGTGGGAACACGTTTTTCAGGTC1000330.6211031648550718No Hit
AGTCTCTCAGCTGGTACACGTCAGGGTCAGGGTTCTGGATATCAGGCCTG889730.5524318163671019No Hit
TCTGATGGCTCAAACACAGCCACCTCGTGTGGGAACACGTTTTTCAGGTC855870.5314082009981809No Hit
TCTGATGGCTCAAACTCAGCTACCTCGTGTGGGAACACGTTTTTCAGGTC827120.5135573757809193No Hit
TCTGATGGCTCAAACACAGCAACCTCGTGTGGGAACACCTTGTTCAGGTC777850.4829657180955461No Hit
TCTGATGGCTCAAACTCAGCGACCTCGTGTGGGAACACGTTTTTCAGGTC761130.47258429904745514No Hit
TCTGATGGCTCAAACTCAGCAACCTCGTGTGGGAACACGTTTTTCAGGTC729450.45291424190370394No Hit
TCTGATGGCTCAAACTCAGCTACCTCGTGTGGGAACACCTTGTTCAGGTC629460.3908306240437391No Hit
TCTGATGGCTCAAACACAGCCACCTCGTGTGGGAACACCTTGTTCAGGTC620740.38541639114306003No Hit
TCTGATGGCTCAAACTCAGCAACCTCGTGTGGGAACACCTTGTTCAGGTC576770.3581154942803472No Hit
TCTGATGGCTCAAACTCAGCGACCTCGTGTGGGAACACCTTGTTCAGGTC547550.33997284687692514No Hit
TCTGATGGCTCAAACTCAGCCACCTCGTGTGGGAACACGTTTTTCAGGTC520180.3229788612700921No Hit
TCTGATGGCTGAAACTCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC516050.3204145514214907No Hit
TCTGATGGCTGAAACTCAGCCACCTCGGGTGGGAACACGTTTTTCAGGTC430770.26746434709007955No Hit
TCTGATGGCTGAAACTCAGCAACCTCGGGTGGGAACACGTTTTTCAGGTC416620.2586786365918447No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTGAGTTCC395400.24550317533583457No Hit
TCTGATGGCTGAAACTCAGCTACCTCGGGTGGGAACACGTTTTTCAGGTC394860.24516789027088426No Hit
TCTGATGGCTCAAACTCAGCCACCTCGTGTGGGAACACCTTGTTCAGGTC392530.24372119730545053No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTTGGTTTT366120.2273232740363069No Hit
TCTGATGGCTGAAACTCAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC361830.22465962046475726No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTGGGTATG342710.21278804557244274No Hit
TCTGATGGCTGAAACTCAGCAACCTCGGGTGGGAACACCTTGTTCAGGTC325830.20230728280140356No Hit
TCTGATGGCTGAAACTCAGCCACCTCGGGTGGGAACACCTTGTTCAGGTC317520.19714761819077942No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT317080.19687442295267177No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATCAGGCCAG301490.1871946189479028No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATTGAGTTCC298600.18540022295215022No Hit
TCTGATGGCTGAAACTCAGCTACCTCGGGTGGGAACACCTTGTTCAGGTC295220.1833015868048687No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTTGGTTTA288630.17920986721593812No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATACGGATGA288620.17920365823325385No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATG270700.1680771612630511No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCC263950.16388609795117232No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG255450.15860846266954715No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATAGGGCTGG255090.1583849392929136No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTTGGAATG251290.15602552587289292No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATAGGACTTG247370.1535916046606611No Hit
TCTGATGGCTGAAACTCAGCAACCTCGTGTGGGAACACGTTTTTCAGGTC243610.15125702717137748No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGACTTG239250.148549910721038No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGA238250.1479290124526115No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATTTGGTTTA236590.14689832132702352No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATTGGGTATG235790.1464016027122823No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATTTGGTTTT224840.13960276667301225No Hit
TCTGATGGCTGAAACTGAGCAACCTCGGGTGGGAACACGTTTTTCAGGTC218090.13541170336113345No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTAAGGGTTCTGGATATCAGGCCTG216160.1342133697030703No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG215930.1340705631013322No Hit
TCTGATGGCTCAAACACAGCGACCTCCGGTGGGAACACGTTTTTCAGGTC213670.13266733301468833No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATTTGGAATG211950.13159938799299478No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTTGGATTC208430.1294138260881335No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA197120.12239146667222991No Hit
TCTGATGGCTCAAACACAGCGACCTCCTGTGGGAACACGTTTTTCAGGTC192490.11951670768941525No Hit
TCTGATGGCTGAAACTCAGCAACCTCGTGTGGGAACACCTTGTTCAGGTC190560.11831837403135215No Hit
TCTGATGGCTGAAACTCAGCTACCTCGTGTGGGAACACGTTTTTCAGGTC185880.11541257013511616No Hit
TCTGATGGCTGAAACTGAGCCACCTCGGGTGGGAACACGTTTTTCAGGTC181260.11254402013498577No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATACGGATGA175930.10923463236427258No Hit
AGTCTCTCAGCTGGTACACGACAGGGTCAGGGTTCTGGATATCAGGCCAG175150.1087503317148999No Hit
AGTCTCTCAGCTGGTACACGACAGGGTAAGGGTTCTGGATATCAGGCCTG172330.1069993985979372No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATCGAGCGTG172140.10688142792693617No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATTTGGCTGG172020.106806920134725No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTG167070.10373347370601387No Hit
TTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGA166680.10349132338132754No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG166480.10336714372764222No Hit
AGTCTCTCAGCTGGTACACGCCAGGGTCAGGGTTCTGGATATCTGGTTTT165730.10290147002632237No Hit
TCTGATGGCTCAAACACAGCAACCTCCTGTGGGAACACGTTTTTCAGGTC163640.101603792645311No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)