FastQCFastQC Report
Wed 12 Jul 2023
EGAF00008107277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008107277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21705387
Sequences flagged as poor quality0
Sequence length259
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTGCCAAGCACCTCTCTCTGCACATTGTGCCCTCCCAGCCTGGAGAC7334743.3792256272601824No Hit
GCCAACCTTGTCATCTCCGCTTCACAACTGGGGGACTCAGCAATGTATTT5598052.579106283615215No Hit
TTATCCCTGCCGACAGAAAGTCCAGCACTCTGAGCCTGCCCCGGGTTTCC5376092.476845955338184No Hit
TCCACTTGGAGAAAGGCTCAGTTCAAGTGTCAGACTCAGCGGTGTACTTC4845102.232210833190857No Hit
GCCAGCCAGTATATTTCCCTGCTCATCAGAGACTCCAAGCTCAGTGATTC4817972.2197116319556986No Hit
GTTCTCTCCACATCACTGCAGCCCAGACTGGTGATACAGGCCTCTACCTC4179741.925669420222731No Hit
TCTGCAAATTGCAGCTACTCAACCTGGAGACTCAGCTGTCTACTTTTGTG3888591.7915322127175157No Hit
AGAAAGTCCAGTACCTTGATCCTGCACCGTGCTACCTTGAGAGATGCTGC3713621.7109208879804816No Hit
AATGGCCTCACTTGATACCAAAGCCCGTCTCAGCACCCTCCACATCACAG3141341.4472628384833681No Hit
GCCAACCTTGTCATCTCCGCTTCACAACTGGGGGACTCAGCAATGTACTT2762481.2727163077073909No Hit
GCCAGCCAGTATGTTTCTCTGCTCATCAGAGACTCCCAGCCCAGTGATTC2533981.1674429025384343No Hit
GGAAACCCTCAGTCCATATAAGCGACACGGCTGAGTACTTCTGTGCTGTG2439921.1241080382487536No Hit
CACCTGACGAAACCCTCAGCCCATATGAGCGACGCGGCTGAGTACTTCTG2307941.0633028565673581No Hit
GCCAAACATTTCTCCCTGCACATCACAGAGACCCAACCTGAAGACTCGGC1791610.8254218180952037No Hit
GAAACCTCCTTCCACCTGACGAAACCCTCAGCCCATATGAGCGACGCGGC1662730.7660448532891858No Hit
AAGGATAAACATCTGTCTCTGCGCATTGCAGACACCCAGACTGGGGACTC1662000.7657085312507904No Hit
GCAGACACAAAGCAAAGCTCTCTGCACATCACAGCCTCCCAGCTCAGCGA1594520.7346194748796693No Hit
GTATCCCTGCCGACAGAAAGTCCAGCACTCTGAGCCTGCCCCGGGTTTCC1416470.6525891475696793No Hit
CCTTCAGTCTCAAGATCTCAGACTCACAGCTGGGGGATGCCGCGATGTAT1310770.6038915592705166No Hit
GCCAGCCAGTATGTTTCTCTGCTCATCAGAGACTCCAAGCTCAGTGATTC1259550.5802937307683111No Hit
GACCTTAACAAAGGCGAGACATCTTTCCACCTGAAGAAACCATTTGCTCA1238440.5705680345621112No Hit
GTTCTCTTCATCGCTGCTCATCCTCCAGGTGCGGGAGGCAGATGCTGCTG1178020.5427316269458822No Hit
GTCACGCTTGACACTTCCAAGAAAAGCAGTTCCTTGTTGATCACGGCTTC967130.44557141505931225No Hit
GCCAGCCAGTATATTTCCCTGCTCATCAGAGACTCCCAGCCCAGTGATTC929580.42827156226240054No Hit
CTGTACCTTACGGCCTCCCAGCTCAGTTACTCAGGAACCTACTTCTGCGG924520.4259403437496876No Hit
TTTCAGCATCCTGAACATCACAGCCACCCAGACCGGAGACTCGGCCGTCT833940.3842087680813984No Hit
GGAACTTCCAGAAATCCACCAGTTCCTTCAACTTCACCATCACAGCCTCA796750.367074772728079No Hit
TTTCAGCATCCTGAACATCACAGCCACCCAGACCGGAGACTCGGCCATCT768540.35407799916214344No Hit
CAAGTGGAAGACTTAATGCCTCGCTGGATAAATCATCAGGACGTAGTACT679470.3130421033266995No Hit
GGTTTACAGCACAGGTCGATAAATCCAGCAAGTATATCTCCTTGTTCATC639470.29461349848311846No Hit
GCTGCAAATTGCAGCTACTCAACCTGGAGACTCAGCTGTCTACTTTTGTG627320.2890158097618808No Hit
AGAAGGAAAGCTTTCTGCACATCACAGCCCCTAAACCTGAAGACTCAGCC522720.24082500809591645No Hit
CTTGTGAGCGACTCCGCTTTGTACTTCTGTGCTGTTCCAGGAACCTACAA396550.1826965812680511No Hit
GTTCAGCATCCTGAACATCACAGCCACCCAGACCGGAGACTCGGCCATCT367880.1694878787464144No Hit
TCATCACAGAAGACAGAAAGTCCAGCACCTTGATCCTGCCCCACGCTACG350590.16152211430277655No Hit
GTTCAGCATCCTGAACATCACAGCCACCCAGACCGGAGACTCGGCCGTCT314500.14489490558265558No Hit
TTGTTTCATATCACAGCCTCCCAGCCTGCAGACTCAGCTACCTACCTCTG314360.14483040546570305No Hit
CATACAGGAAAAGCACAGCTCCCTGCACATCACAGCCTCCCATCCCAGAG311870.14368322481419013No Hit
GCCACTTGGAGAAAGGCTCAGTTCAAGTGTCAGACTCAGCGGTGTACTTC304120.14011268262574633No Hit
CCTTCAGTCTCAAGATCTCAGACTCACAGCTGGGGGACACTGCGATGTAT297330.13698442695354845No Hit
CTTGTGAGCGACTCCGCTTTGTACTTCTGTGCTGTGAGAGAGGCGGACAC270020.12440229699659353No Hit
TCATCACAGAAGACAGAAAGTCCAGCACTCTGAGCCTGCCCCGGGTTTCC251780.11599885318792058No Hit
GCAGACACAAAGCAAAGCTCTCTGCACATCACAGCCTCCCAGCTCAGCGT248190.11434488590320918No Hit
TCTGCAAATTGCAGCTACTCAACCTGGAGACTCAGCTGTCTACTTGACCA244910.11283374030603556No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)