FastQCFastQC Report
Wed 12 Jul 2023
EGAF00008107284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008107284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5871243
Sequences flagged as poor quality0
Sequence length35
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCGTATTGCGGTGTTATTTCATTGGTTTTTTT897081.5279217705688557No Hit
AGGGTTTAGTTCGGGAGGTATATTGCTATTTTTTT711821.2123838171916919No Hit
GACGCCGTAAAGGCTAGGGCCCCTTATCTTTTTTT706781.2037996042745973No Hit
GGTGCTGATCACAAAGCCAATTAGAGTGTTTTTTT663571.130203604245302No Hit
GGTCTTGAGCGCTCTTAGGAAAAGAGGTTTTTTTT519940.8855705682765983No Hit
GCAGACCCAGCACGTAAAATAGGTCACTTTTTTTT494820.8427857610390168No Hit
TTACAGACCTAAATGAAATGATCATCCGTTTTTTT457810.7797497054712265No Hit
TCCGATGGTGCGACATGTTTCTCGTCAGTTTTTTT421050.7171394541155935No Hit
TCCCGCCTATGTGCGTTAATAGGTGGTATTTTTTT408470.6957129861598302No Hit
AAATGCTCGTTACGTTCCGTCTGTTTCTTTTTTTT407110.6933966112456936No Hit
CTGGGATAAATAATGGACACCGCCAGAGTTTTTTT377510.6429813925262504No Hit
CAGCTCGTGCTTGTGTCATTCTAAGCATTTTTTTT376540.6413292721830794No Hit
TTGAGAAGTTTAGCATTTATCTATCATGTTTTTTT376170.6406990819490864No Hit
AGCATTACGAGGCAAGATAGAAAAATACTTTTTTT365020.6217082140868637No Hit
CTTAGTAGGCCTACAGACCACGGTTTCGTTTTTTT354470.603739276333819No Hit
TTGAATTCACGTGAGGGTAACTTCTAATTTTTTTT351850.5992768481904087No Hit
ACCTAATCGACTTCCAGGTGCTTTCGCTTTTTTTT348490.5935540395790125No Hit
TACCTCAGTTGTCTGTCGTTTAAAGTTTTTTTTTT345910.5891597401095474No Hit
TCAGCCAATCCGTAAAGGGTTAAACGGGTTTTTTT338200.5760279382066114No Hit
TGCTCTTGAGAAGAAGAATACTATGTCTTTCTTTA336940.5738818849773378No Hit
TGCTCCACAGTTCTTAGTTCAATTATGGTTTTTTT335520.5714633170522835No Hit
CTATGGGTCTCAACAACGTGTCAGTAATTTTTTTT331430.5644971601413875No Hit
CTATCGACGAAATACAGCGACGTCAGAGTTTTTTT328580.5596429921227923No Hit
ACTCCCTAATGCTAAATCGTGGGTTGCCTTTTTTT323530.5510417470372118No Hit
AGCAGAAGGAGAAAGAACAGATAGAAAATTTTTTT322080.5485720826066984No Hit
TGCATGGATCGGATCTACAACGATGGCATTTTTTT319480.5441437188002609No Hit
GAAGTGATTTATCGTGTCCAAGAGCATTTTTTTTT318150.5418784403915832No Hit
CCCTCGATAATGAGATAATTTCCCCCCACGTCTTG316900.5397494193307959No Hit
GATCGCTACCCGATTTGTTTGGCTACGTTTTTTTT305830.5208948088164636No Hit
TCCTACATCCACGGCCTCTCGAGCGGGTTTTTTTT280130.47712213580667673No Hit
AAGTTCACTCCAAGCTTTGACTAGATTGTTTTTTT279400.4758787875071769No Hit
CGAACGCCCAGTGCCGATCTTTTGTTAGTTTTTTT277500.4726426754947803No Hit
CGCTCGACATAATGATGACCTAAAGATTTTTTTTT269930.4597493239506523No Hit
TAGGTGAGCCCTACTCTCCCTTCTTCGTTTTTTTT269800.4595279057603305No Hit
TCACGGTCATCGCACAACAAACTGCTAATTTTTTT263440.4486954466030447No Hit
GGATGAAGATCGCTGACAAGCAGGGCCATTTTTTT259850.44258089811646356No Hit
TCGCTGTGCGTAAATCCCTTTAGAAACTTTTTTTT257540.43864646719612865No Hit
ACCGACACATCTCCCAGGATTAACAGTATTTTTTT252890.4307265088499999No Hit
TAGCTCGCCTGATAACTAAGTTCAGGGTTTTTTTT246320.41953637415450185No Hit
CGAGCACTTCAAGTTTAAGGGTAGTAGCTTTTTTT243000.41388169421705084No Hit
ACACCGAGCGCTCTTTTAAAGATTGTATTTTTTTT234620.3996087370255328No Hit
CCCAGGAAGAATTCGACAGAGACATAACTTTTTTT232390.3958105634530882No Hit
AGGTATGCGGACATTATATCCCCACTTCTTTTTTT229610.3910756206138973No Hit
AGTGAACAAACTTCTCGAAGTGCCTTGCTTTTTTT227490.387464800894802No Hit
AGTCAAGATGACACTTGAAGGTAATATCTTTTTTT225340.3838028846702478No Hit
TATTCGTGCCAGAATAGTCGCTAATGCATTTTTTT214260.36493124198742927No Hit
CATGACTTCGCTGAATATAAGGGACGCATTTTTTT212370.36171216214351887No Hit
ATCTACCATCTGCTCCGTGCTGGTATATTTTTTTT209140.3562107717224444No Hit
TGATCACCACACTGACTTGCTGCAGTTTTTTTTTT208450.3550355520968899No Hit
TGACCCACGTTAGACAGTGATTCATGGATTTTTTT205680.3503176414261852No Hit
CACGCACAGCGCAGCTGCCTTTTGAATTTTTTTTT204070.34757546229989117No Hit
TAGTCGATCACGGGTTAGTCGTATGTCGTTTTTTT199460.3397236326277076No Hit
GTTCCAGTCTGACCATGGCAGGCTTTGGTTTTTTT198810.3386165416760982No Hit
TCCTCGGGCTGGGCTTGCTTATTCACTTTTTTTTT198560.33819073746394074No Hit
CTTTGGCGCTTTATACGGCTTTGGTGCTTTTTTTT193580.32970871755776415No Hit
GATTCCTATACGGCGCACTAATTTTATTTTTTTTT190270.3240710697887994No Hit
GGATGAAGATCGCTGAGTAAAAGAGGTGTTTTTTT189200.32224862776076546No Hit
TCGGGAGACAGCGTACTTTTCAACACGTTTTTTTT188930.3217887592116354No Hit
GTAGACGTCGTTACATCGGTAAAATGGATTTTTTT186890.31831419684043055No Hit
TTATCCGGGATCTATACGCTTTGGTATGTTTTTTT180560.30753283418860367No Hit
ACTATCTGCCCGCGTAATTTAAAGGGTTTTTTTTT180540.30749876985163105No Hit
GCAGATCCATAAGACTTTAAAATAGAATTTTTTTT180220.3069537404600695No Hit
CCTATAATGAGTGCCCCTTACAAGATGGTTTTTTT180210.3069367082915832No Hit
GGTCGGCCAGGAGCTTCACTGGGCGGTATTTTTTT179800.306238389383645No Hit
GACGGGTTGGCCCGTACGCGAAGAACGTTTTTTTT179760.3061702607096998No Hit
GCGTCGCCAGGGTGATACGGCTTAATTATTTTTTT179410.30557413481267937No Hit
GAAGTTTCCACTCAATGATCGAGATGAGTTTTTTT178860.30463736554593296No Hit
GTAGTCGCGGGAATCATTGCAACCGTGTTTTTTTT176560.3007199667940843No Hit
CCTATAATGAGTGCCCAGATTGTTAAGTTTTTTTT175180.29836952754297513No Hit
AGAGCAGTTATGAGACCCGTAAAATCAATTTTTTT175170.2983524953744889No Hit
CACATTTCTTGTCAGAGGTACGAGGTCGTTTTTTT168570.28711126417353194No Hit
CAGTACATTCTCTAAAATAAATAGAGGGTTTTTTT168320.28668545996137446No Hit
ATGCGACAGTCCCATTTGGTGTTGGTTGTTTTTTT166880.2842328276993475No Hit
TAAGTTGCGACGTAGGCCCAGGAGGTGGTTTTTTT163610.2786633086043279No Hit
CCATGCTCTGCAGGAAACGCTCTACACTTTTTTTT162740.27718150994602No Hit
TAATAGTGACGACCAGGTACGATAATTATTTTTTT162600.27694305958721177No Hit
ATCTTATCGCACACCCCGGTTCATTTTCTTTTTTT161600.27523984273858193No Hit
GAGATCTGTCACTCCGGATACACTTGGTTTTTTTT160970.27416681612394517No Hit
AGAGCGGGCTAATCATTGCCAATATTGGTTTTTTT160130.2727361139710961No Hit
GTAGACGTCGTTACATTCCATTTAGCGTTTTTTTT159220.2711861866388429No Hit
AGAAGGTTGTAGGTCGGTGCGGCCAGTTTTTTTTT157750.268682457871357No Hit
TCGGAGTACATGAGTAATATAGGTTCTGTTTTTTT154610.26333435696665936No Hit
TTGGACCTATAACAGTCCGAGATATACATTTTTTT152950.2605070169979338No Hit
GCGAGGCCCGAGCAGATAAAGCAGATCTTTTTTTT151840.2586164462959547No Hit
CGTTCAGACCCGCGAAAGAAACACACTCTTTTTTT151790.2585312854535232No Hit
CGGAAAGAATCAAACGTGGCCTCTCGTTTTTTTTT149270.25423917899497606No Hit
AGCAGAAGGAGAAAGATAAGCTTTATACTTTTTTT148370.2527062838312092No Hit
GAGCGCGCACGAGTAGACTGCTGACATTTTTTTTT148030.252127190102675No Hit
GTAGACACGCCTGACTCTGGGTCGCTTATTTTTTT147280.25084977746620263No Hit
TTATCGCCTGCGAAGCGTTGATCGGTGGTTTTTTT145800.24832901653023048No Hit
GGCTGGCTAGCTTAAAATTGCCCGTTTGTTTTTTT145770.24827792002477161No Hit
TCCGATGGTGCGACATGCCTAGGCGATTTTTTTTT145650.24807353400293602No Hit
GTAGTCTACGATATTGTTTCGTGTTTTTTTTTTTT143950.24517806536026526No Hit
TACGCTATAGAAACCTTGAAGGCATTGTTTTTTTT143030.24361110585952583No Hit
CCCGCCATGCTCCCGTTAATTTGGTCTATTTTTTT141540.24107331275506735No Hit
ATGGGCCTCGGCCTCTATGTGCACCGGGTTTTTTT139760.23804158676450624No Hit
AGAAGGTTGTAGGTCGGTGCGCCCAGTTTTTTTTT139600.23776907206872547No Hit
ATCCAACGCAGTCATACACATGTGTAAATTTTTTT139570.2377179755632666No Hit
CACTGACGATTGTGGATGGTAGTTTCCCTTTTTTT139340.2373262356880817No Hit
TGGCAAGCACAAGTCGCAATTATTTTTGTTTTTTT138990.2367301097910613No Hit
TATTCCGAGCTGTTATCTATTAGTCATTTTTTTTT138680.23620211256798604No Hit
AGATACGACTTCATATTGATAAAAGCGATTTTTTT137160.2336132229580687No Hit
CTTACACGGTATTCCAAAGCTGAGTGTGTTTTTTT136830.23305116139802082No Hit
GACACAAGGGAAGAAAGTTTGGGTTTGATTTTTTT135840.23136497671787729No Hit
TCCGTTTAGCCTTGAACCTATTTCGCTCTTTTTTT135400.23061556130448013No Hit
AATGACTTTCAGCCGGTGAGCATGTTGCTTTTTTT134550.2291678269831448No Hit
AGTCTTCTCCTCAAATTAGGACTGTAGGTTTTTTT133000.22652784086776853No Hit
CCAGGGACGTGGCCTCTGGAATGATTCATTTTTTT132960.22645971219382335No Hit
ACGTTAGATTTGCCCGCGAGTACAGCCCTTTTTTT132060.2249268170300565No Hit
TGCGGAGTAAAGGTGCTGAAAGTTCAAGTTTTTTT131970.22477352751367982No Hit
TGCATGTGGTAATCTAGTAGTCCAGGGATTTTTTT131750.22439881980698123No Hit
TTAAGCGCCTGACCCAAAATCTCTTTCTTTTTTTT131360.22373456523601562No Hit
TGTTGTCAAGAAGTCTAATTAGGTAGGCTTTTTTT131170.22341095403477593No Hit
GTAGAAACGGGTGGAGTTAGGCGACCTATTTTTTT131010.22313843933899516No Hit
CATGATGCACAATTCTAATCCTTTTTAGTTTTTTT131010.22313843933899516No Hit
TCGGACGCCCAGCCCATGGGTACAACCTTTTTTTT130990.2231043750020226No Hit
ATTAGGCGATGCTTTCCGGTTCAGTTTGTTTTTTT130820.2228148281377555No Hit
CATTATGCTTGTTGTGTGGTTAAGGAACTTTTTTT125100.2130724277635928No Hit
ACCCTCCCTTGCTATTAGGAAATCCGTGTTTTTTT122940.20939347937055236No Hit
AAGCGCAGGGCTTTGACTGGAGCCGGTATTTTTTT122140.20803090589164847No Hit
AAGACTGCAAGCTACTTTTGATGATAGATTTTTTT121660.2072133618043062No Hit
CAGCTCGTGCTTGTGTGGATTGTGTTAATTTTTTT119360.20329596305245756No Hit
GGCGCGGAGATCTTTCTTGCATATTTGGTTTTTTT118190.20130319933956065No Hit
GTGAAACGTGCTCCACGCTCTTTACGTTTTTTTTT117270.19973623983882116No Hit
ACCTACTATAAATCTACATTTAGATTCCTTTTTTT117130.199497789480013No Hit
GGCTTTCAATAAGGGTTGGTGGTGAACGTTTTTTT117100.19944669297455414No Hit
TGTTGTCAAGAAGTCTCCTGGGTGCACGTTTTTTT116950.19919121044725963No Hit
ACGATACATAGAACTACGGCGGTTCGTTTTTTTTT116220.19794786214775983No Hit
GCTCAATGTAATACCGGCCGTCTCTTTTTTTTTTT115790.19721547890284905No Hit
GGCTTCTCGTGGGTGGTACCTGTTTTATTTTTTTT115230.1962616774676163No Hit
CTAAAGAATGCCTACTCGAGTTTTATCGTTTTTTT114720.1953930368748151No Hit
CTGCCATGCATCACATTTGTAATTTGTATTTTTTT114260.1946095571244454No Hit
CCCGAGTTTCTCCGTATAATTTTAACCTTTTTTTT114150.19442220327109608No Hit
GGTGGACTGCTCTGGCCCTCTGTGCGGGTTTTTTT111420.18977242127433663No Hit
ATAGGGATATCCTTGACAGCTGTGTGAGTTTTTTT111280.18953397091552845No Hit
TCCGATAATTGCCATACGCTTATTGCAGTTTTTTT108830.18536108963638534No Hit
TGCGCGATTAACGGAGGAATCTCGGTCTTTTTTTT108160.18421993434780334No Hit
CGATTAAATATCTCCTCGTAGGGTAGTCTTTTTTT107620.18330019724954325No Hit
GTCCGGCTGAATTGCGTGATGGTGTGTTTTTTTTT107230.1826359426785776No Hit
CGCTTAGTATTGATACACGCCTCCTGTATTTTTTT106970.18219310629793384No Hit
AACTGGGTCCCGACGTTCCATGATTAAATTTTTTT106520.1814266587160504No Hit
TAGAATAGCCGATGAAGGAGTTCATGCGTTTTTTT105860.18030253559595472No Hit
ACGTATTACTCCGATCATCACCATATCCTTTTTTT105460.17962124885650277No Hit
ACGTGCGCCTCGTGCATAGCAGGGCCTCGATAATG105140.17907621946494123No Hit
TGTGTTCGTATCCAAGGGTTTGGCTCTCTTTTTTT103470.1762318473277294No Hit
TCACAGGTTATTGGGCGTACCGTGATGATTTTTTT103270.17589120395800345No Hit
TCCCAGCTTTAGTCTGGTATAAGACGGGTTTTTTT101860.17348966820143538No Hit
TACGAGAACTTCACGTGGCCCTAGAAAGTTTTTTT100940.17192270870069593No Hit
CCACAGTACCCATCCTTATTTCCATATCTTTTTTT100090.17047497437936054No Hit
AAGGCGCGTAAAGCTTATTTTACATATTTTTTTTT99930.17020245968357978No Hit
CTACCCTAAGGTCATACGTTGGCTGGTTTTTTTTT98020.1669493155026968No Hit
CTACCCTAAGGTCATATAATAGAAGTTGTTTTTTT96730.1647521657679643No Hit
CTTCTTACGTCGTATACTAGAGTATGGGTTTTTTT96180.1638153965012179No Hit
CCAAGCGTAACTCGTATTCTCTGCGCGATTTTTTT95700.16299785241387557No Hit
CACCCTAACAAGATCTTACGACCCAATCTTTTTTT95490.1626401768756633No Hit
TAAGGCCCGTCACCCTTTCGCGATTGTTTTTTTTT94330.16066444533125268No Hit
ACCGACACATCTCCCAGCATGCGTTAGTTTTTTTT93420.15911451799899953No Hit
TATTTGTTACCCTTTAAGACACATGGACTTTTTTT93000.158399166922575No Hit
GCCCGCGCGTAAACGGGGTCCACTGTGGTTTTTTT91700.1561849850193562No Hit
TGCTCTTGAGAGTTTGATACCAATCAATTTTTTTT91010.15500976539380162No Hit
TCGCAAAGATGCATTTCGTACGGTATTTTTTTTTT90690.15446473600224006No Hit
AACCATGGGATCGCTAAGGTCTATGCCATTTTTTT90650.15439660732829488No Hit
GTGCGGGTCTCCAAATGTACCGTTCTTGTTTTTTT86100.14664697066702911No Hit
TCTAGTGATATCGTGGTTCCCCTAGTGATTTTTTT85910.14632335946578945No Hit
CCGGAGCGTACTTTCTTCAAGCTCGTAATTTTTTT85720.14599974826454978No Hit
ACGTTTCGTACACACGGCACGAGTTCTTTTTTTTT85390.1454376867045019No Hit
CTAACGAAACTTGCTGTTGAAGCTTGTGTTTTTTT84390.1437344698558721No Hit
TCCTCTACGAGATGGCGTAAAGGGTCTTTTTTTTT83710.1425762823988038No Hit
AGCAGAAGGAGAAAGAATCTATGGCCACTTTTTTT83530.1422697033660504No Hit
ATATTGCTGTCAAAGTTAACCCTATTTTTTTTTTT82670.14080493687622875No Hit
ATTAATGAACCAGTCGGCCCCAAGCTGGTTTTTTT82460.1404472613380165No Hit
GGCGCGGAGATCTTTCGACTGCACACCTTTTTTTT82240.14007255363131793No Hit
CTAGATGTGAGTGTAATCTTGTAACTAATTTTTTT82090.13981707110402344No Hit
ACGCGGGCCAAGGACAATTTGTGTTTAATTTTTTT82020.13969784592461937No Hit
AGGGTTTAGTCGGGAATGACATGATAGTTTTTTTT81970.13961268508218788No Hit
GCTAATACCGAATGCCCATGTACTGACATTTTTTT81130.1381819829293388No Hit
TCCTTACGACGGTCCGAGTTCTTGGAGATTTTTTT80930.13784133955961286No Hit
AAATTGGTGAGAAGCATTTCCGTCAACATTTTTTT79460.135337610792127No Hit
ACTGTACGATACACATGAACGCTATCTTTTTTTTT79060.13465632405267505No Hit
GTGCGACAGGGAGTGTATGGTCATCTTGTTTTTTT78500.13370252261744234No Hit
ATCAGCCTCATGCTGCGGTTGCACCTCCTTTTTTT78140.1330893645519356No Hit
AGGGCTGCAGTTACAGGCTGTAAATTTTTTTTTTT77240.13155646938816873No Hit
CTCATTCACGACAAATACGTTAATTATATTTTTTT76750.13072189313234012No Hit
TTGCTGCACCTATCCAAGGGGATTTCATTTTTTTT75720.12896757977825138No Hit
GCGTCTCTGCATTGGGCCCGTATGATTCTTTTTTT74030.12608914330406695No Hit
TCCAGGGTATATACGAGGACTATATAATTTTTTTT72100.12280193478621138No Hit
GCTATACGTCTCGGACTTCCTGTTGTTTTTTTTTT71760.12222284105767724No Hit
CTATCGGGTCTCAACACCAGTTTACATATTTTTTT71650.12203548720432794No Hit
CTATCGACGAAATACATGGGATCAGTGGTTTTTTT70180.11953175843684208No Hit
CATGCGTTAGACAGAATAATTTCGTGATTTTTTTT69990.11920814723560241No Hit
ACTCAGACCTGCTTCTCCTTTTCGGAGTTTTTTTT67930.11569952052742495No Hit
CCCAAACATGCTGCTCAGACCATGCGGATTTTTTT66480.11322985609691168No Hit
TCCTTGTCCTTTAATTTTTGATCCTTGTTTTTTTT66450.11317875959145278No Hit
GCGCATCCAGTCAGCACTGGATATTTGTTTTTTTT65620.11176508960709002No Hit
GGGAGGTGCCCGAAATAGATATCTGACTTTTTTTT65300.11122006021552847No Hit
TTCCTCGAGGGTGTCTCTCATATCTACTTTTTTTT64950.11062393431850802No Hit
AATATCGAGGGTTCTCGTGTCATACGCATTTTTTT64670.11014703360089166No Hit
GTCTATCTGAGTTTCTGTCTCTTTACTTTTTTTTT64410.1097041972202479No Hit
TGTTCTTCCATTGACTAAGGTACAAATTTTTTTTT63660.10842678458377554No Hit
TGTATGGCGCAGACAGTACCACAGATGGTTTTTTT62410.10629776352298823No Hit
TGTATGGCGCAGACAGGTCTGGCTGTGTTTTTTTT62230.10599118449023485No Hit
ACTGTCTTCTTTAGAATTAGTTTAAGACTTTTTTT62090.10575273413142669No Hit
ATCCTGAATCGCTGCGACGGATATTGCCTTTTTTT61840.10532692991926923No Hit
TCGGACGCCCAGCCCACCAGGCAACGCATTTTTTT61530.10479893269619397No Hit
TGTTCGTATTGCGGTGCGGTCTGAGTCTTTTTTTT61250.10432203197857763No Hit
TGGTAGAATATATGGGGATCTATACAGTTTTTTTT61150.10415171029371463No Hit
CCCTCGATAATACACACGAGCGTCTGAGTTTTTTT60340.10277210464632446No Hit
TAAGGCCCGTCACCCTTCGGCTGACGGTTTTTTTT59920.10205675356989993No Hit
CGACACGCTCCGACAGTCTTCTTTGGCTTTTTTTT59830.10190346405352325No Hit
GTAGTCTACGATATTGGCGATAGTTTAATTTTTTT59730.10173314236866027No Hit
CTGAGCAAGTAACAAGTAGAACAACGGCTTTTTTT59430.10122217731407131No Hit
GAGATCTGTCACTCCGCACTGGAAGGCGTTTTTTT59190.10081340527040014No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)