FastQCFastQC Report
Wed 12 Jul 2023
EGAF00008107286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008107286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6249383
Sequences flagged as poor quality0
Sequence length35
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCAAGGCCCGGCTTGTATAGTTTGTTTTTTTT2668484.269989533366734No Hit
CAAATGTCCTTCCGTGACTTTGGTACTCTTTTTTT759101.2146799132010313No Hit
ATCGACTCTTTCCGTTTTAGAAAGTTGTTTTTTTT699871.1199025567804053No Hit
ATCAAACACTGTTCCACCTTGGGTCTGTTCCCTTA637141.0195246474731987No Hit
AAGAGGATGTACGCGATGAGCATAACTTTTTTTTT635731.0172684247388903No Hit
TCCGCTGTCATCCCACTGAAGATCCATCATCTGTG621770.9949302195112703No Hit
CGTACCTGATAGGCCTATCGTGCACAAGTTTTTTT483260.7732923394197475No Hit
CGTGGAAGCCTCGTACAGCCGCCGTTCGTTTTTTT478930.766363655420063No Hit
TACACCTCTTCTCCCTCTGTTTGGAGAGGGAGAAG457290.7317362370013167No Hit
GGTCTTGAGCGCTCTTCAAGGCATATTTTTTTTTT442550.708149908558973No Hit
CGTGCATTGTCGACGCTGGGTTAGATGGTTTTTTT430500.6888680050494584No Hit
CATTACGCAGGAAGGGGCCATATTTACATTTTTTT426930.6831554411051458No Hit
CGCCTCCCTCCTCTGCATTGCCCCTCTTCTCCCTC413910.6623213843670648No Hit
ACTTACGCATCCACGCATCAGGTATGCCTTTTTTT410570.6569768567552989No Hit
TACGTTTACCGGCAATAAAATGACGTGGTTTTTTT396300.6341426025577245No Hit
CACGTTCGTGCTCTAGTAGGGTATTTAGTTTTTTT376160.60191542109037No Hit
TGTCCACGGCTCAACTGGTTCTCCGGGTTTTTTTT374420.5991311462267555No Hit
CGATAATGAGATAATTTCCCCCCACGTCTTGAGAA371250.5940586454694807No Hit
CACACAGGGATAGATTCCAATAGGGCTGTTTTTTT371210.593994639150777No Hit
AGTTAAACACTTGCGAGGCTTGATCCGTTTTTTTT363960.5823934938857164No Hit
ATACCTAACCAAGAAAATTTCTGATGGATTTTTTT359810.575752838320199No Hit
TTCTAGGCCAATTGTGTCACTGTTTGTTTTTTTTT358650.5738966550777893No Hit
TGTCCACGGCTCAACTTCTTTCCTGTTTTTTTTTT333800.5341327295830645No Hit
CACACAGCTTGCGCTCTGGTAGGTAGATTTTTTTT333290.5333166490195912No Hit
CGCCCAGCGTTTCACGTATATGTACCGGTTTTTTT333140.5330766253244521No Hit
GCGACGATAGTTGTACAATCGCTTCATGTTTTTTT323190.5171550535468862No Hit
GTGAGCGTGCTGCACTCGTGTACGCTAGTTTTTTT320890.5134746902214187No Hit
AGCTCTTCGTAACCTTTGAACAATGTGATTTTTTT312680.5003373933074673No Hit
GAAGCTTGCTGACCGCACGAGATCATATTTTTTTT307430.4919365639775959No Hit
CCTGCTATTTGAGAAGGAGCGAGAAACATTTTTTT305910.48950432386685216No Hit
GGATGTCCTTACCGCAATGAGATAATTTCCCCCCA305480.4888162559407864No Hit
GGATTAATCATGGACCAGTGTTTCAAAATTTTTTT296500.47444683739178733No Hit
TATATCCCTGGGAGGATTGCCCAGGATGTTTTTTT293850.47020641877766167No Hit
GAAAGCAGTGCACTCTTTTCCTAGGATTTTTTTTT290390.46466987220978456No Hit
CTGCTCTGACGGCAAAATTTAAAGCTTTTTTTTTT289650.46348575531376457No Hit
GCATTTCCAAGGCTCCGTTACAGCAAGTTTTTTTT287480.46001341252408434No Hit
CCGCACTTGCAATGACGTTGGTGACATTTTTTTTT283530.45369278855208584No Hit
TAAAGCGTTAGGAGAAATACAGTTAATTTTTTTTT277150.44348378071883254No Hit
GGACACAAGTTTACACCAAACCTACTGGTTTTTTT270910.4334987950010425No Hit
CCTCTAATCTGCCAAGCCAGGTTGACATTTTTTTT262190.4195454175236179No Hit
TAGGGTGTTTCAAGAGCGGGCGTCACTATTTTTTT258830.41416888675250024No Hit
ATACAGGCCCTCCAATATGTGTGAGGTGTTTTTTT257160.41149662294661726No Hit
TTCCTCGAGGGTGTCTTCGTCTTTTAATTTTTTTT252530.4040878915566545No Hit
GTTGCGCTAACATTACTCGCGTTAGCTTTTTTTTT243540.38970247142797937No Hit
TACGCCATATTCTAATCTGGCTGGGACATTTTTTT234610.3754130607773599No Hit
TGATCGGTTTGACCCTGAACAATGTGGTTTTTTTT233800.37411693282360836No Hit
TGTGGCGGGCTTCTGGCAGCAGGGCCTCGATAATG230860.36941246839888037No Hit
GGAGTGCCGCCCTGGACATATGACCACGTTTTTTT227190.3635398886578083No Hit
CATGACTTCGCTGAATGAGCAATCGCGTTTTTTTT217720.3483863927046878No Hit
TGTAGCCAATTCCGTTGAGACTCGCCAGTTTTTTT217490.34801835637214107No Hit
AAACCCGAACGAAATCCCAAAACGGATATTTTTTT215850.345394097305286No Hit
GACCGCGTCTGACGTGGATCACGGGAGGTTTTTTT211050.3377133390608321No Hit
AGGCTATGGTTAGCTTATATTGTGTTTGTTTTTTT206710.3307686534814717No Hit
ATAAACGTTGCACCACGTCCGTCAGTGTTTTTTTT206230.3300005776570263No Hit
CGGCAAACATCGTGCGTGAACTGGTACATTTTTTT196830.3149590927616374No Hit
GATACGATGGGAGTCAGGACAAATTACTTTTTTTT194000.31043064571334483No Hit
GTGTCGTATAGCGTTCATCTGCAGTGCGTTTTTTT193270.3092625303970008No Hit
GCCGAAATTCCTACGTCATCTTCTTATTTTTTTTT192170.30750235663264675No Hit
GTATGTGGGTCTAGTTGCTAGGTTGTGGTTTTTTT192070.3073423408358873No Hit
AATTGCAGCAATCGACAGTGGGGGCAAATTTTTTT191790.3068942966049608No Hit
GGCTTTCAATAAGGGTGGAACGGCGCTGTTTTTTT191410.30628623657727494No Hit
TACACCTCTTCGAATCCTGTAAAGCGTTTTTTTTT189550.303309942757549No Hit
GATAGGTAACGTTGACGAAGATGATGGATTTTTTT188150.3010697216029167No Hit
GTACGAGATTGCGACAGTAAATTGAATGTTTTTTT187000.29922953994018286No Hit
CACACAGCTTGCGCTCTCATAAATTTACTTTTTTT184010.2944450676170752No Hit
CGTTAAACTAGTTAGGGAGAATAGATGATTTTTTT182650.29226885278114656No Hit
TGTCCACGGCTCAACTGTGTAGCGATTGTTTTTTT182470.29198082434697953No Hit
GTCTATCTGAGTTTCTTTTCACGCGCGGTTTTTTT182380.29183681012989604No Hit
GTACAGAGGCAAGGGTACGTCATATCCGTTTTTTT182170.2915007769567012No Hit
GGAGGCCGAAGTCGTCGTTTTCGTTGATTTTTTTT180210.2883644673402158No Hit
CCAAATAACAAGATTCCTACGCAGCACGTTTTTTT177440.2839320297699789No Hit
CCACGGTGCCCGGTAGTGATCAATATTGTTTTTTT174930.2799156332713165No Hit
TAGTCTAACAACGAGACTGTGGAGGGGGTTTTTTT173850.27818746266631444No Hit
AACCGCTAAGGGATGCTTGAGATGCTCTTTTTTTT172720.27637928416293256No Hit
AACGTGCGAAAGTCTCAACAGAATATGGTTTTTTT172220.2755792051791353No Hit
AGAACCCAGCGTGACACATGGCGTAACCTTTTTTT171120.27381903141478126No Hit
AGGCTTCCCGAAGAAGCGACAATGTGCTTTTTTTT169440.2711307660292224No Hit
CAATGCGAGAAGTATCACAGGGAGCTCATTTTTTT168800.2701066649299619No Hit
TTGCCAAGCAGAACCCCCGGAGACCTTTTTTTTTT168720.2699786522925543No Hit
CCGAGAAGTCGCATAAGCGGGAGTCATTTTTTTTT168240.269210576468109No Hit
GTCCGGGTTCACATTACGCTTTTGGACGTTTTTTT168060.26892254803394194No Hit
TTCAAAGTCTCTAGCCACATTGCTGGAGTTTTTTT167940.26873052907783057No Hit
TCTTGGTAACACCAAACTAATACTCAGATTTTTTT167190.26753041060213467No Hit
CCGGGTTCGAGGTTACTTGGCAATAAGGTTTTTTT166020.265658225780049No Hit
TTCCACACAGATTTGAATAGCGTAAGGTTTTTTTT165800.2653061910271782No Hit
TGATGTCAATTAAGTGGGCCGTAGATGCTTTTTTT164720.2635780204221761No Hit
GGCAGCAAACCTATGCTGCTAACTCGATTTTTTTT162620.2602176886902275No Hit
CCAACGATGCACTGATAACACACAGCATAGCAGGG161270.25805747543397484No Hit
GTGACTTCAGTAGTGCCCGTGCGGACATTTTTTTT159780.25567324006225894No Hit
GAGTATCAAAGTTACATTAGATTGGGTTTTTTTTT159690.2555292258451754No Hit
AGTCGACGGTCTCAAGCTGTGGTAATGTTTTTTTT158910.25428110263045167No Hit
CATCTAGTGAAGGGAATTTAATTGGATTTTTTTTT156470.2503767171895209No Hit
GACATCCGTCGAACTGGACGTAATGTGATTTTTTT155230.2483925213097037No Hit
AGTTCCTACAGAATTAGCAGAAGTTTGGTTTTTTT154650.24746442968849885No Hit
TAGTCTAACAACGAGATTCTCACACCTCTTTTTTT154360.24700038387789644No Hit
GAGCGCAAATACTCCGCATTGGGTCGACTTTTTTT152020.24325601423372517No Hit
AAGCACCCTGCGTATCTATAGAATTATTTTTTTTT151820.24293598264020624No Hit
AGTGAGTCGAATTAACATCTTTTCGATGTTTTTTT143730.22999070468236624No Hit
ATCCACATCGACAGAACCTTGTGTCAATTTTTTTT143540.22968667466852327No Hit
TAATTACGTCAGTAGATAAGGCTTTCAGTTTTTTT143510.22963866992949544No Hit
TACTGAACAGATTTAGGTTTCGGATAGATTTTTTT143050.22890259726440193No Hit
CTGCAGAGAATCAGAGGTTTGAGGGGATTTTTTTT142750.22842254987412358No Hit
CAATAAACCTTGGCCCTCAGGAGGGTAATTTTTTT142290.2276864772090301No Hit
CTAAGGGAATGATTGGGTCCCTGATGGTTTTTTTT140620.22501421340314717No Hit
TCACGGTCATCGCACAGAGAGTTACATGTTTTTTT139930.2239101044055069No Hit
ACTCCGGCCGACCACTATAAGTTGTGTCTTTTTTT138750.22202191800374532No Hit
CCAGTCTTGTCATAGAATAGTTAATATTTTTTTTT138150.22106182322318857No Hit
AAAGTGTGATTTATCTTCTTGTCCCTGTTTTTTTT136310.2181175325628146No Hit
GAGTAAACCGGAAAGTTTAGTGCAGTGTTTTTTTT135720.21717343936193378No Hit
TACGCCATATTCTAATTGGGTGACGATGTTTTTTT133560.21371709815192955No Hit
GCTACAATCGAGGATAAGCTCGTCATGGTTTTTTT132510.2120369322859553No Hit
TCGGCAGGGTTAAGGGTCTGGACCGCATTTTTTTT129160.2066764030945135No Hit
GTCTCAAGGCCCGGCTTGTATAGTTTTTTTTTTTT128670.20589232569039215No Hit
CTGTTCACTGCCTGTGTTAAGCAGCACCTTTTTTT128620.20581231779201242No Hit
TATGGCCCGGCCTCGCTTTGTATTCTGCTTTTTTT127250.2036201013764079No Hit
TCTGCTTAGAACAAGCCCTTATTCCGGATTTTTTT126660.20267600817552708No Hit
CACATATTAGCAGGATTGATACGTTTAATTTTTTT125150.2002597696444593No Hit
TAGTCTAACAACGAGATCTGGCCAGCAGTTTTTTT124860.19979572383385688No Hit
ATCTCGTGAGCGAAACATTGTCCGCCTGTTTTTTT124490.1992036653858469No Hit
CCGCTATCAGCACCAGGATTAGTGAGTATTTTTTT124490.1992036653858469No Hit
GACAACGACCATTGAAGATTTGACGTACTTTTTTT124020.19845159114107747No Hit
AAGGATCGATCGCTTGCGTGACATCATATTTTTTT120320.19253100666097758No Hit
CACTCTTCTGCTAGCCGGTCCTGGCTGTTTTTTTT120110.1921949734877827No Hit
GCAACAGCAGTATGCGAATATGAGTCGGTTTTTTT119070.19053080920148438No Hit
CGCTACGGGACATTTATAGGGTGGTTTCTTTTTTT117480.18798655803300904No Hit
GATACGATGGGAGTCATCTGGCCCATCGTTTTTTT116910.18707446799148011No Hit
TCCTGCGTTGATACTCTTATTCGTAGTTTTTTTTT115990.18560232266129312No Hit
GGACACAAGTTTACACAATGATGGCATTTTTTTTT114890.18384214889693912No Hit
AATTCGATTCGAGGATATGCATACCAGATTTTTTT112770.18044981400563861No Hit
GCGACATGTAAACATCCTGCATGCTAATTTTTTTT111380.1782255944306822No Hit
TAGAATAGCCGATGAAACACATTCCAAATTTTTTT108640.1738411615994731No Hit
CCAGGGACGTGGCCTCTTTAAAGACTTTTTTTTTT108040.17288106681891635No Hit
ATATCGGTAGGGAGATTGGTTTCTTTCTTTTTTTT106930.17110489147488642No Hit
TTGCAAGAAGACTCCTTATTTTCTTTGCTTTTTTT106560.17051283302687642No Hit
ATACTAGCATGACCCTACTAGTACTGATTTTTTTT106340.17016079827400563No Hit
CAACCTACCGAGCAGTTAACCTGAACTTTTTTTTT106230.16998478089757021No Hit
GTTAACTATGTTGTCAGTGCATTTGTGGTTTTTTT105490.16880066400155022No Hit
TTGAGCAGCCCACGGTGTGCATCATATCTTTTTTT105440.16872065610317052No Hit
CGACTTTGTATAGCCTATACACTGTATGTTTTTTT103870.16620840809404702No Hit
AGAGCCGCCGAGATTTCGGTACGTTAATTTTTTTT103370.16540832911024975No Hit
AGCTCTTCGTAACCTTTCTTAAGAACCATTTTTTT103360.1653923275305738No Hit
CGGAACGTAAACATAGACGATTTAGTATTTTTTTT101180.16190398316121768No Hit
AAATCTAGCCCTGCTATAGTCCGCAGGTTTTTTTT100390.16063985836681796No Hit
TTCGGTACTGTAGAGGTATTATAAGGTGTTTTTTT99070.15852764984959314No Hit
GCCACTCCTTACGGTAATATGGCGTTTTTTTTTTT97690.15631943185431266No Hit
TCTGCCAGAAACTGCAGGATTGCTTCATTTTTTTT96480.15438324071352325No Hit
CAGCCTCGATAGCGGTAACTGCTAATGATTTTTTT96100.15377518068583731No Hit
CTAAAGGATGAGATACATGTTCGCAGGTTTTTTTT95770.1532471285565311No Hit
TTGCACGGAGCAGCACTCGACCCTCACTTTTTTTT94650.15145495163282519No Hit
TTGGTCACACTCGTAATATTCTGTATCATTTTTTT93720.14996680472296225No Hit
GCAAACGTCGCCAGGTCTACCAAGTTTATTTTTTT91210.1459504082242999No Hit
CAATGGATCTCTACCAGGTTTTATAATTTTTTTTT90990.1455983734714291No Hit
CACAATGAGCTGCTATATATGATATGCTTTTTTTT90510.1448302976469837No Hit
TGGGTGTAATAGATTTGTCAGGTTTCTTTTTTTTT90060.14411022656156616No Hit
AATAACAACGCTCGGCAGTGAGGTGAATTTTTTTT89860.14379019496804724No Hit
GTGTGAATAACTTAGGTTTGGTCGGATCTTTTTTT89770.14364618075096372No Hit
ACTCTCTGACTTAGGTGCTCCTTCCTGATTTTTTT89530.14326214283874103No Hit
ACGCCGCTAGACGACCTTGTTACGGAACTTTTTTT87010.13922974476040273No Hit
GGCCTGCTCTGATGTTCATCTGATAAGTTTTTTTT87000.1392137431807268No Hit
AGTCAAGATGACACTTTCTGCGGAGTACTTTTTTT86760.1388297052685041No Hit
GCCGCTTGTGAGAAACTCATTAATACACTTTTTTT85840.1373575599383171No Hit
CAATTAAGGGTGATGAGTCTAATTTTTTTTTTTTT85700.13713353782285387No Hit
TAAGGGCCTGTCCGATTGTGATTGTTGATTTTTTT84300.13489331666822146No Hit
ATAGTTCCACCCACTCTGTTCCCTTATTGCTGCTT83670.1338852171486369No Hit
TAACATCGAAGGTCCCCGTTTTCGTACTTTTTTTT83580.1337412029315534No Hit
ACAAAGCATGACCTAGCATCGTCGGTTTTTTTTTT82890.13263709393391315No Hit
ATCTCCTTCGCACATCTGGTTGGAGTAGTTTTTTT82190.13151698335659698No Hit
TCTTACGGCATCCGACCTCCTGATAGTATTTTTTT82080.13134096598016157No Hit
TACGCTCGGTATTGGAACAAAATGGGATTTTTTTT82020.13124495650210588No Hit
TACGGGTAATAACATACCTACCATGAGATTTTTTT81840.13095692806793885No Hit
GAGTAAACCGGAAAGTAAATGCATCCGCTTTTTTT81810.13090892332891102No Hit
TACTGGACAGCTCGGCCACTAGTGTGATTTTTTTT81650.13065289805409588No Hit
CGCTACGGGACATTTATTCACGATCGTATTTTTTT81110.12978881275159485No Hit
TGGCAGCAGTAATAGTGTTGGCTTTGTGTTTTTTT81080.12974080801256702No Hit
CCGGAATGGTTTCAGTTAACATGACTTATTTTTTT79610.12738857580020302No Hit
CACAGGGCCGTTGTCAAGACTTCTACGATTTTTTT79250.12681251893186896No Hit
AGGTAGGTACAAAGCTTTCTTATTGGTATTTTTTT78080.12494033410978332No Hit
CAGAGACGGTCACCCATAGCAAGGGAATTTTTTTT77430.12390023143084684No Hit
CGGAACGTAAACATAGCAGCGATTAGAATTTTTTT77400.12385222669181901No Hit
GGATTTCACTTCTATAAAAATTTGCTGATTTTTTT76770.12284412717223445No Hit
CGCAGGCGATCCAAACGTCGTAACGTTCTTTTTTT76530.12246008926001174No Hit
GTGTGAATAACTTAGGTATGTTGTTTTTTTTTTTT76240.12199604344940933No Hit
TCTAGGTGGCGACGCTATAGATACGGTATTTTTTT75390.12063590917695394No Hit
AGGAAGCTGTCCGCCGAGTCCCCAGTTTTTTTTTT75310.1205078965395464No Hit
ATGAAGCCAAGGAGCCGTGAGGGCTTTTTTTTTTT75170.12028387442408314No Hit
GTGTCGTATAGCGTTCCGCGCTGGGTTTTTTTTTT74950.11993183967121235No Hit
CGGGTGTACCCATTTAATATGCAAGGTTTTTTTTT74490.11919576700611884No Hit
TTGGATATCGTCTACGAAATTGTTCGAGTTTTTTT71780.1148593389139376No Hit
TACTCGTTTGAATCAATAGTCGGATGTTTTTTTTT71420.11428328204560355No Hit
AAGCTCGTGCCAAGTCTCAAGCCATATTTTTTTTT71390.11423527730657572No Hit
TTCCGGTATCTGTGTCACAATAGTACATTTTTTTT71090.11375522991629733No Hit
GTAGACACGCCTGACTCCCGGGTCCAGATTTTTTT71020.11364321885856572No Hit
CCCAGTTAAGGCGCCGTATGGTGGGAACTTTTTTT70680.11309916514958356No Hit
TGGTTCGTAGCAAAGGCGTGGCTGGGTATTTTTTT70480.11277913355606464No Hit
GATATGAGACACTAACCTCAGTTGCTTCTTTTTTT70450.11273112881703681No Hit
TCAGCCAATCCGTAAATTTCCGACGTGCTTTTTTT70340.11255511144060142No Hit
GTGTTACTATGCGTCCCGTGAACGGGTATTTTTTT70130.11221907826740657No Hit
CCAGTCTTGTCATAGAAAGTGATGTATATTTTTTT70110.11218707510805466No Hit
AAGTAGTGACGCGAGGCCATTCGCATCGTTTTTTT69000.1104108997640247No Hit
TCCGGGTGTGCTGCTTCTAAGGACTGCTTTTTTTT68810.11010686975018173No Hit
GCACAAACGAGGCGTGGGACGGCTTATTTTTTTTT67520.10804266597198475No Hit
CCCTGAAATGAGTTGAATAAGGTGGGCCTTTTTTT66740.106794542757261No Hit
GCACTGCCCCTCTTCTCCCTCTGTTTGGAGAGGGA66720.10676253959790911No Hit
CTACTGCCACCTGACCTATGTTGCCTTATTGCTGC65180.10429829632781348No Hit
TCTGTGCCATCATAGTTTGTATCAGGGATTTTTTT64780.10365823314077566No Hit
GGCATACAGGTAGCGGCCAACTTTACAGTTTTTTT63270.10124199460970788No Hit
AGTGATTCAAGCAGGAAGTTGCGAAACATTTTTTT63180.10109798039262437No Hit
CCTGGAAACGTTCTGCCATTAACCTGCTTTTTTTT62690.10031390298850304No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)