FastQCFastQC Report
Wed 12 Jul 2023
EGAF00008107287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008107287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6249383
Sequences flagged as poor quality0
Sequence length259
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAAGTCCAGTACCTTGATCCTGCACCGTGCTACCTTGAGAGATGCTGC4321016.914298579555774No Hit
GTTCTCTCCACATCACTGCAGCCCAGACTGGTGATACAGGCCTCTACCTC3557005.691761890733853No Hit
AAAGTGCCAAGCACCTCTCTCTGCACATTGTGCCCTCCCAGCCTGGAGAC1565502.505047298269285No Hit
GCCAGCCAGTATGTTTCTCTGCTCATCAGAGACTCCCAGCCCAGTGATTC1315052.104287735285227No Hit
GCCAACCTTGTCATCTCCGCTTCACAACTGGGGGACTCAGCAATGTACTT1313182.1012954398858255No Hit
GCCAGCCAGTATATTTCCCTGCTCATCAGAGACTCCAAGCTCAGTGATTC1223911.9584493381186592No Hit
AAGGATAAACATCTGTCTCTGCGCATTGCAGACACCCAGACTGGGGACTC1222041.9554570427192572No Hit
TCCACTTGGAGAAAGGCTCAGTTCAAGTGTCAGACTCAGCGGTGTACTTC983551.5738353690276305No Hit
TCTGCAAATTGCAGCTACTCAACCTGGAGACTCAGCTGTCTACTTTTGTG866231.3861048362694366No Hit
GCCAACCTTGTCATCTCCGCTTCACAACTGGGGGACTCAGCAATGTATTT595310.9525900396887181No Hit
TTTCAGCATCCTGAACATCACAGCCACCCAGACCGGAGACTCGGCCGTCT521790.834946425911166No Hit
TTATCCCTGCCGACAGAAAGTCCAGCACTCTGAGCCTGCCCCGGGTTTCC453510.7256876398838094No Hit
CTGTACCTTACGGCCTCCCAGCTCAGTTACTCAGGAACCTACTTCTGCGG432080.6913962546382579No Hit
GCCAGCCAGTATATTTCCCTGCTCATCAGAGACTCCCAGCCCAGTGATTC430580.6889960176868661No Hit
CACCTGACGAAACCCTCAGCCCATATGAGCGACGCGGCTGAGTACTTCTG377750.6044596722588453No Hit
GCCAGCCAGTATGTTTCTCTGCTCATCAGAGACTCCAAGCTCAGTGATTC349540.5593192159930028No Hit
AATGGCCTCACTTGATACCAAAGCCCGTCTCAGCACCCTCCACATCACAG330960.5295882809550959No Hit
GGTTTACAGCACAGGTCGATAAATCCAGCAAGTATATCTCCTTGTTCATC287060.4593413461776947No Hit
GCAGACACAAAGCAAAGCTCTCTGCACATCACAGCCTCCCAGCTCAGCGA275170.44031546794299536No Hit
GTATCCCTGCCGACAGAAAGTCCAGCACTCTGAGCCTGCCCCGGGTTTCC262380.4198494475374609No Hit
CCTTCAGTCTCAAGATCTCAGACTCACAGCTGGGGGATGCCGCGATGTAT246170.3939108868827531No Hit
TTGTTTCATATCACAGCCTCCCAGCCTGCAGACTCAGCTACCTACCTCTG239170.38270978110959114No Hit
GCCAAACATTTCTCCCTGCACATCACAGAGACCCAACCTGAAGACTCGGC239120.38262977321121144No Hit
GAAACCTCCTTCCACCTGACGAAACCCTCAGCCCATATGAGCGACGCGGC236400.3782773435393542No Hit
GTTCAGCATCCTGAACATCACAGCCACCCAGACCGGAGACTCGGCCGTCT209090.33457702944434675No Hit
TCATCACAGAAGACAGAAAGTCCAGCACCTTGATCCTGCCCCACGCTACG200740.3212157104149322No Hit
GCTGCAAATTGCAGCTACTCAACCTGGAGACTCAGCTGTCTACTTTTGTG198320.3173433281333533No Hit
GTTCTCTTCATCGCTGCTCATCCTCCAGGTGCGGGAGGCAGATGCTGCTG192410.3078863945448695No Hit
CATACAGGAAAAGCACAGCTCCCTGCACATCACAGCCTCCCATCCCAGAG192350.3077903850668138No Hit
GGAACTTCCAGAAATCCACCAGTTCCTTCAACTTCACCATCACAGCCTCA192330.3077583819074619No Hit
GGAAACCCTCAGTCCATATAAGCGACACGGCTGAGTACTTCTGTGCTGTT181090.28977260635169905No Hit
GTGTTTCATATCACAGCCTCCCAGCCTGCAGACTCAGCTACCTACCTCTG171370.2742190709066799No Hit
GACTGTCGCTACGGAACGCTACAGCTTATTGTACATTTCCTCTTCCCAGA168940.2703306870454251No Hit
GGAAACCCTCAGTCCATATAAGCGACACGGCTGAGTACTTCTGTGCTGTG165050.2641060725514823No Hit
GGAAACCCTCAGTCCATATAAGCGACACGGCTGAGTACTTCTGTGCTCCG136730.2187895989092043No Hit
GTCACGCTTGACACTTCCAAGAAAAGCAGTTCCTTGTTGATCACGGCTTC122180.19550730048070344No Hit
GACCTTAACAAAGGCGAGACATCTTTCCACCTGAAGAAACCATTTGCTCA117880.18862662122004686No Hit
GAAGGAGCTCCAGATGAAAGACTCTGCCTCTTACCTCTGTGCTGTGAGGG107990.17280105892053665No Hit
AGAAGGAAAGCTTTCTGCACATCACAGCCCCTAAACCTGAAGACTCAGCC101930.1631041016369136No Hit
TTTCAGCATCCTGAACATCACAGCCACCCAGACCGGAGACTCGGCCATCT98580.1577435724454718No Hit
GCCACTTGGAGAAAGGCTCAGTTCAAGTGTCAGACTCAGCGGTGTACTTC94780.1516629721686125No Hit
CCTTCAGTCTCAAGATCTCAGACTCACAGCTGGGGGACACTGCGATGTAT90570.1449263071250394No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG76560.12250809399903957No Hit
GTTCTCTCCACATCACTGCAGCCCAGCCTGGTGATACAGGCCTCTACCTC70860.11338719358375059No Hit
CTTGTGAGCGACTCCGCTTTGTACTTCTGTGCTGTGAGAGACAGCTGGGG69040.1104749060827285No Hit
AATCTGAGGAAACCCTCTGTGCAGTGGAGTGACACAGCTGAGTACTTCTG65800.1052903942677221No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)