FastQCFastQC Report
Wed 12 Jul 2023
EGAF00008107288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008107288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7025970
Sequences flagged as poor quality0
Sequence length35
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCGTATTGCGGTGTTATTTCATTGGTTTTTTT890111.2668855688253722No Hit
GGTGCTGATCACAAAGCCAATTAGAGTGTTTTTTT742441.0567081840656876No Hit
GACGCCGTAAAGGCTAGGGCCCCTTATCTTTTTTT741751.0557261132626525No Hit
AAATGCTCGTTACGTTCCGTCTGTTTCTTTTTTTT563130.8014978714682812No Hit
GCACTGCCCCTCTTCTCCCTCTGTTTGGAGAGGGA553910.7883751282741032No Hit
GCAGACCCAGCACGTAAAATAGGTCACTTTTTTTT536830.7640653176714389No Hit
GGTCTTGAGCGCTCTTAGGAAAAGAGGTTTTTTTT469300.6679504751657067No Hit
CTATGGGTCTCAACAACGTGTCAGTAATTTTTTTT445790.6344889033115712No Hit
CTGGGATAAATAATGGACACCGCCAGAGTTTTTTT443750.6315853896330329No Hit
TCCGATGGTGCGACATGTTTCTCGTCAGTTTTTTT430890.6132818671300901No Hit
TCCTACATCCACGGCCTCTCGAGCGGGTTTTTTTT425220.6052118070529763No Hit
TCCCGCCTATGTGCGTTAATAGGTGGTATTTTTTT423920.6033615287284175No Hit
ACCTAATCGACTTCCAGGTGCTTTCGCTTTTTTTT422680.6015966478649923No Hit
TTACAGACCTAAATGAAATGATCATCCGTTTTTTT422600.6014827845834809No Hit
CTTAGTAGGCCTACAGACCACGGTTTCGTTTTTTT422030.6006715087027129No Hit
CAGCTCGTGCTTGTGTCATTCTAAGCATTTTTTTT397660.5659859065723309No Hit
TTGAATTCACGTGAGGGTAACTTCTAATTTTTTTT392340.558413998351829No Hit
AGCATTACGAGGCAAGATAGAAAAATACTTTTTTT391790.5576311882914388No Hit
TCACGGTCATCGCACAACAAACTGCTAATTTTTTT388490.5529343279290974No Hit
TCAGCCAATCCGTAAAGGGTTAAACGGGTTTTTTT375370.5342607497612429No Hit
AGCAGAAGGAGAAAGAACAGATAGAAAATTTTTTT371560.528838010979267No Hit
CTATCGACGAAATACAGCGACGTCAGAGTTTTTTT366580.521750021705188No Hit
ACTCCCTAATGCTAAATCGTGGGTTGCCTTTTTTT365660.520440593967808No Hit
GAAGTGATTTATCGTGTCCAAGAGCATTTTTTTTT362460.5158860627073557No Hit
TACCTCAGTTGTCTGTCGTTTAAAGTTTTTTTTTT355690.5062503825094613No Hit
TGCTCCACAGTTCTTAGTTCAATTATGGTTTTTTT354200.5041296788913132No Hit
CGAACGCCCAGTGCCGATCTTTTGTTAGTTTTTTT352200.5012830968535306No Hit
TTGAGAAGTTTAGCATTTATCTATCATGTTTTTTT343450.4888293004382313No Hit
GATCGCTACCCGATTTGTTTGGCTACGTTTTTTTT342320.4872209815868841No Hit
GGATGAAGATCGCTGACAAGCAGGGCCATTTTTTT337440.4802753214146943No Hit
AAGTTCACTCCAAGCTTTGACTAGATTGTTTTTTT336510.4789516607671254No Hit
TGCTCTTGAGAAGAAGAATACTATGTCTTTCTTTA335580.4776280001195564No Hit
CCCTCGATAATGAGATAATTTCCCCCCACGTCTTG328340.4673233731427831No Hit
TGCATGGATCGGATCTACAACGATGGCATTTTTTT326800.46513150497369044No Hit
CGCTCGACATAATGATGACCTAAAGATTTTTTTTT304800.4338191025580809No Hit
ACCGACACATCTCCCAGGATTAACAGTATTTTTTT300980.428382130865916No Hit
TAGGTGAGCCCTACTCTCCCTTCTTCGTTTTTTTT296500.4220057871012828No Hit
AGTGAACAAACTTCTCGAAGTGCCTTGCTTTTTTT293830.41820560008084295No Hit
ATCTACCATCTGCTCCGTGCTGGTATATTTTTTTT280360.3990338700563765No Hit
TGATCACCACACTGACTTGCTGCAGTTTTTTTTTT270860.3855126053769088No Hit
TCGCTGTGCGTAAATCCCTTTAGAAACTTTTTTTT267500.38073034755343393No Hit
ACACCGAGCGCTCTTTTAAAGATTGTATTTTTTTT261290.3718917103261187No Hit
TAGCTCGCCTGATAACTAAGTTCAGGGTTTTTTTT256880.36561499693280786No Hit
AGGTATGCGGACATTATATCCCCACTTCTTTTTTT255530.3636935540573045No Hit
CGAGCACTTCAAGTTTAAGGGTAGTAGCTTTTTTT254960.3628822781765365No Hit
TAGTCGATCACGGGTTAGTCGTATGTCGTTTTTTT254520.3622560301282243No Hit
CACGCACAGCGCAGCTGCCTTTTGAATTTTTTTTT248230.35330352961939776No Hit
GGTCGGCCAGGAGCTTCACTGGGCGGTATTTTTTT247150.3517663753189951No Hit
AGTCAAGATGACACTTGAAGGTAATATCTTTTTTT243770.34695565167514236No Hit
TTATCCGGGATCTATACGCTTTGGTATGTTTTTTT241950.3443652620207601No Hit
CATGACTTCGCTGAATATAAGGGACGCATTTTTTT239380.34070740410220934No Hit
GTTCCAGTCTGACCATGGCAGGCTTTGGTTTTTTT239040.3402234851557863No Hit
GCAGATCCATAAGACTTTAAAATAGAATTTTTTTT235390.3350284729368329No Hit
TGACCCACGTTAGACAGTGATTCATGGATTTTTTT232500.3309151618922369No Hit
TATTCGTGCCAGAATAGTCGCTAATGCATTTTTTT229700.3269299470393412No Hit
GTAGACACGCCTGACTCTGGGTCGCTTATTTTTTT229680.32690148121896334No Hit
GATTCCTATACGGCGCACTAATTTTATTTTTTTTT228060.32459574976835937No Hit
CACATTTCTTGTCAGAGGTACGAGGTCGTTTTTTT221900.3158282770919887No Hit
CCCAGGAAGAATTCGACAGAGACATAACTTTTTTT216980.3088256852790433No Hit
AGAGCAGTTATGAGACCCGTAAAATCAATTTTTTT214950.30593640451069387No Hit
GGATGAAGATCGCTGAGTAAAAGAGGTGTTTTTTT213880.30441348312048017No Hit
TTATCGCCTGCGAAGCGTTGATCGGTGGTTTTTTT211670.30126800996873027No Hit
GACGGGTTGGCCCGTACGCGAAGAACGTTTTTTTT210950.3002432404351285No Hit
CCTATAATGAGTGCCCCTTACAAGATGGTTTTTTT210810.30004397969248375No Hit
TAAGTTGCGACGTAGGCCCAGGAGGTGGTTTTTTT208150.29625802558223274No Hit
GTAGACGTCGTTACATCGGTAAAATGGATTTTTTT207300.29504822821617516No Hit
GAAGTTTCCACTCAATGATCGAGATGAGTTTTTTT203110.2890846388470204No Hit
ACTATCTGCCCGCGTAATTTAAAGGGTTTTTTTTT202780.28861495281078625No Hit
CCATGCTCTGCAGGAAACGCTCTACACTTTTTTTT201260.28645155046207144No Hit
CCCGCCATGCTCCCGTTAATTTGGTCTATTTTTTT198140.28201088248313044No Hit
GAGATCTGTCACTCCGGATACACTTGGTTTTTTTT197390.28094341421896196No Hit
CCTATAATGAGTGCCCAGATTGTTAAGTTTTTTTT197190.28065875601518364No Hit
CGTTCAGACCCGCGAAAGAAACACACTCTTTTTTT196830.28014637124838276No Hit
TCGGGAGACAGCGTACTTTTCAACACGTTTTTTTT195610.2784099562053354No Hit
ATCTTATCGCACACCCCGGTTCATTTTCTTTTTTT195290.27795450307929015No Hit
CAGTACATTCTCTAAAATAAATAGAGGGTTTTTTT194660.2770578297373886No Hit
TCCTCGGGCTGGGCTTGCTTATTCACTTTTTTTTT191880.27310108070487066No Hit
CTTTGGCGCTTTATACGGCTTTGGTGCTTTTTTTT189020.2690304683908414No Hit
TTGGACCTATAACAGTCCGAGATATACATTTTTTT188150.26779220520440594No Hit
AGAAGGTTGTAGGTCGGTGCGGCCAGTTTTTTTTT187760.26723712170703834No Hit
ATGCGACAGTCCCATTTGGTGTTGGTTGTTTTTTT184850.2630953448420645No Hit
GTAGTCGCGGGAATCATTGCAACCGTGTTTTTTTT183310.26090347667297187No Hit
CGGAAAGAATCAAACGTGGCCTCTCGTTTTTTTTT183120.2606330513793825No Hit
TAATAGTGACGACCAGGTACGATAATTATTTTTTT177220.25223563436792357No Hit
GTAGTCTACGATATTGTTTCGTGTTTTTTTTTTTT173840.24742491072407086No Hit
GCGTCGCCAGGGTGATACGGCTTAATTATTTTTTT172250.2451618780040336No Hit
CTTACACGGTATTCCAAAGCTGAGTGTGTTTTTTT170940.24329736676928596No Hit
ATCCAACGCAGTCATACACATGTGTAAATTTTTTT169610.24140438971416048No Hit
ACGTTAGATTTGCCCGCGAGTACAGCCCTTTTTTT169180.2407923745760372No Hit
AGAGCGGGCTAATCATTGCCAATATTGGTTTTTTT169140.24073544293528154No Hit
TCCGATGGTGCGACATGCCTAGGCGATTTTTTTTT168980.2405077163722589No Hit
GACACAAGGGAAGAAAGTTTGGGTTTGATTTTTTT166180.2365225015193632No Hit
TACGCTATAGAAACCTTGAAGGCATTGTTTTTTTT165610.2357112256385951No Hit
AGATACGACTTCATATTGATAAAAGCGATTTTTTT164580.23424523588913704No Hit
AGAAGGTTGTAGGTCGGTGCGCCCAGTTTTTTTTT163370.2325230537562785No Hit
AATGACTTTCAGCCGGTGAGCATGTTGCTTTTTTT162580.23139865385135433No Hit
GTAGACGTCGTTACATTCCATTTAGCGTTTTTTTT162280.2309716665456869No Hit
TGCGGAGTAAAGGTGCTGAAAGTTCAAGTTTTTTT162200.23085780326417565No Hit
ACCCTCCCTTGCTATTAGGAAATCCGTGTTTTTTT161940.23048774759926388No Hit
CACTGACGATTGTGGATGGTAGTTTCCCTTTTTTT160540.22849514017281597No Hit
ATAGGGATATCCTTGACAGCTGTGTGAGTTTTTTT159730.227342274447514No Hit
TCGGAGTACATGAGTAATATAGGTTCTGTTTTTTT156990.22344245705575175No Hit
AAGCGCAGGGCTTTGACTGGAGCCGGTATTTTTTT156610.22290160646857302No Hit
CTAAAGAATGCCTACTCGAGTTTTATCGTTTTTTT155940.22194800148591584No Hit
GGCTGGCTAGCTTAAAATTGCCCGTTTGTTTTTTT155670.22156371291081517No Hit
AAGACTGCAAGCTACTTTTGATGATAGATTTTTTT154940.22052471046702446No Hit
AGTCTTCTCCTCAAATTAGGACTGTAGGTTTTTTT154060.21927221437040012No Hit
CCAGGGACGTGGCCTCTGGAATGATTCATTTTTTT153560.2185605688609544No Hit
GAGCGCGCACGAGTAGACTGCTGACATTTTTTTTT152800.217478867686597No Hit
ACCTACTATAAATCTACATTTAGATTCCTTTTTTT152720.21736500440508572No Hit
TCCGTTTAGCCTTGAACCTATTTCGCTCTTTTTTT152620.21722267530319656No Hit
GCGAGGCCCGAGCAGATAAAGCAGATCTTTTTTTT151750.2159844121167611No Hit
GTGAAACGTGCTCCACGCTCTTTACGTTTTTTTTT151020.21494540967297043No Hit
CTACCCTAAGGTCATACGTTGGCTGGTTTTTTTTT150970.21487424512202585No Hit
ACGATACATAGAACTACGGCGGTTCGTTTTTTTTT150580.21431916162465825No Hit
TCCGATAATTGCCATACGCTTATTGCAGTTTTTTT149600.21292433642614472No Hit
ATGGGCCTCGGCCTCTATGTGCACCGGGTTTTTTT149470.21273930859368884No Hit
ATTAGGCGATGCTTTCCGGTTCAGTTTGTTTTTTT149120.21224115673707686No Hit
CATGATGCACAATTCTAATCCTTTTTAGTTTTTTT147250.20957960253175006No Hit
CAGCTCGTGCTTGTGTGGATTGTGTTAATTTTTTT145910.20767239256643566No Hit
AGCAGAAGGAGAAAGATAAGCTTTATACTTTTTTT145030.2064198964698113No Hit
TGCATGTGGTAATCTAGTAGTCCAGGGATTTTTTT144860.20617793699659975No Hit
CGCTTAGTATTGATACACGCCTCCTGTATTTTTTT141330.2011537196999133No Hit
TCGGACGCCCAGCCCATGGGTACAACCTTTTTTTT137590.19583061128925972No Hit
GGTGGACTGCTCTGGCCCTCTGTGCGGGTTTTTTT137370.19551748726510362No Hit
TGGCAAGCACAAGTCGCAATTATTTTTGTTTTTTT137220.1953039936122699No Hit
AGGGTTTAGTTCGGGAGGTATATTGCTATTTTTTT136710.19457811519263532No Hit
TGTGTTCGTATCCAAGGGTTTGGCTCTCTTTTTTT136690.1945496493722575No Hit
GTAGAAACGGGTGGAGTTAGGCGACCTATTTTTTT135940.19348218110808899No Hit
CGATTAAATATCTCCTCGTAGGGTAGTCTTTTTTT135290.1925570419458096No Hit
ACGTATTACTCCGATCATCACCATATCCTTTTTTT135130.19232931538278702No Hit
TGTTGTCAAGAAGTCTAATTAGGTAGGCTTTTTTT134910.1920161913586309No Hit
CGGCGCCATCCCACTGAAGATCCATCATCTGTGGG133420.18989548774048282No Hit
ATTAATGAACCAGTCGGCCCCAAGCTGGTTTTTTT133320.18975315863859368No Hit
TAGAATAGCCGATGAAGGAGTTCATGCGTTTTTTT133250.18965352826727128No Hit
CTTAGTTGAGGAATCGCTACTGCTTCTTTTTTTTT132420.18847219672159146No Hit
CTACCCTAAGGTCATATAATAGAAGTTGTTTTTTT131800.18758975628987884No Hit
TTAAGCGCCTGACCCAAAATCTCTTTCTTTTTTTT131150.18666461712759946No Hit
CATTATGCTTGTTGTGTGGTTAAGGAACTTTTTTT130480.18571101214494226No Hit
TATTTGTTACCCTTTAAGACACATGGACTTTTTTT130260.18539788812078617No Hit
TATTCCGAGCTGTTATCTATTAGTCATTTTTTTTT128960.18354760979622742No Hit
TACGAGAACTTCACGTGGCCCTAGAAAGTTTTTTT126460.17998938224899907No Hit
TGTTGTCAAGAAGTCTCCTGGGTGCACGTTTTTTT126320.1797901215063543No Hit
AACTGGGTCCCGACGTTCCATGATTAAATTTTTTT124880.17774058243915075No Hit
CTTCTTACGTCGTATACTAGAGTATGGGTTTTTTT123630.17596146866553655No Hit
GGCGCGGAGATCTTTCGACTGCACACCTTTTTTTT123120.175235590245902No Hit
CCCGAGTTTCTCCGTATAATTTTAACCTTTTTTTT121430.17283022842397563No Hit
ACGTGCGCCTCGTGCATAGCAGGGCCTCGATAATG121370.17274483096284213No Hit
ATATTGCTGTCAAAGTTAACCCTATTTTTTTTTTT121110.1723747752979304No Hit
CCACAGTACCCATCCTTATTTCCATATCTTTTTTT121020.17224667910623018No Hit
TAAGGCCCGTCACCCTTTCGCGATTGTTTTTTTTT120780.17190508926169626No Hit
CCAAGCGTAACTCGTATTCTCTGCGCGATTTTTTT120170.17103688174017254No Hit
AAGGCGCGTAAAGCTTATTTTACATATTTTTTTTT118960.16931469960731402No Hit
ATCAGCCTCATGCTGCGGTTGCACCTCCTTTTTTT118730.168987342672969No Hit
GGCTTCTCGTGGGTGGTACCTGTTTTATTTTTTTT118240.16828993007371224No Hit
TCCCAGCTTTAGTCTGGTATAAGACGGGTTTTTTT118020.16797680604955617No Hit
TCACAGGTTATTGGGCGTACCGTGATGATTTTTTT117200.16680970741406526No Hit
GGCTTTCAATAAGGGTTGGTGGTGAACGTTTTTTT115750.1647459354366728No Hit
CACCCTAACAAGATCTTACGACCCAATCTTTTTTT115420.16427624940043864No Hit
TCCTCTACGAGATGGCGTAAAGGGTCTTTTTTTTT114630.1631518494955145No Hit
ACCGACACATCTCCCAGCATGCGTTAGTTTTTTTT111810.1591381688222409No Hit
GCTCAATGTAATACCGGCCGTCTCTTTTTTTTTTT110120.15673280700031456No Hit
GGCGCGGAGATCTTTCTTGCATATTTGGTTTTTTT110030.15660471080861432No Hit
CTGCCATGCATCACATTTGTAATTTGTATTTTTTT109520.15587883238897973No Hit
TGCGCGATTAACGGAGGAATCTCGGTCTTTTTTTT107490.15298955162063033No Hit
TTGCTGCACCTATCCAAGGGGATTTCATTTTTTTT106070.15096847837380462No Hit
CTAACGAAACTTGCTGTTGAAGCTTGTGTTTTTTT105530.1501999012236033No Hit
GGACCTCTAGGCCGCCCTATGGCTTTTCTTTTTTT105170.1496875164568024No Hit
ACGTTTCGTACACACGGCACGAGTTCTTTTTTTTT103630.14749564828770975No Hit
AAATTGGTGAGAAGCATTTCCGTCAACATTTTTTT102680.14614352181976298No Hit
GCCCGCGCGTAAACGGGGTCCACTGTGGTTTTTTT102550.1459584939873071No Hit
GTCCGGCTGAATTGCGTGATGGTGTGTTTTTTTTT102280.14557420541220645No Hit
GTGCGGGTCTCCAAATGTACCGTTCTTGTTTTTTT101610.14462060042954925No Hit
AGCAGAAGGAGAAAGAATCTATGGCCACTTTTTTT101280.1441509143933151No Hit
CTAGATGTGAGTGTAATCTTGTAACTAATTTTTTT100850.1435388992551918No Hit
ACGCGGGCCAAGGACAATTTGTGTTTAATTTTTTT100240.1426706917336681No Hit
TGCTCTTGAGAGTTTGATACCAATCAATTTTTTTT99200.1411904690740211No Hit
CCGGAGCGTACTTTCTTCAAGCTCGTAATTTTTTT99000.14090581087024281No Hit
GCTAATACCGAATGCCCATGTACTGACATTTTTTT97470.13872817561133907No Hit
CCCAAACATGCTGCTCAGACCATGCGGATTTTTTT97350.1385573806890721No Hit
GCTATACGTCTCGGACTTCCTGTTGTTTTTTTTTT96960.13800229719170448No Hit
CTCATTCACGACAAATACGTTAATTATATTTTTTT96650.13756107697584818No Hit
AATATCGAGGGTTCTCGTGTCATACGCATTTTTTT95760.1362943479690349No Hit
CTATCGACGAAATACATGGGATCAGTGGTTTTTTT94990.13519841388448856No Hit
TCTAGTGATATCGTGGTTCCCCTAGTGATTTTTTT93830.1335473963025746No Hit
ACTCAGACCTGCTTCTCCTTTTCGGAGTTTTTTTT93790.13349046466181894No Hit
AGGGTTTAGTCGGGAATGACATGATAGTTTTTTTT93730.13340506720068546No Hit
TCCTTACGACGGTCCGAGTTCTTGGAGATTTTTTT93490.13306347735615154No Hit
ACTGTACGATACACATGAACGCTATCTTTTTTTTT90580.1289217004911777No Hit
TCGCAAAGATGCATTTCGTACGGTATTTTTTTTTT89130.1268579285137853No Hit
TAAGGCCCGTCACCCTTCGGCTGACGGTTTTTTTT88010.125263842572627No Hit
AGCAACCGAAAGTAATCGGTCCAGTTGATTTTTTT88010.125263842572627No Hit
TGTATGGCGCAGACAGAACTTCTTAACGTTTTTTT87100.12396864774543587No Hit
TGTATGGCGCAGACAGGTCTGGCTGTGTTTTTTTT86930.12372668827222433No Hit
TCCAGGGTATATACGAGGACTATATAATTTTTTTT86280.12280154910994495No Hit
CTATCGGGTCTCAACACCAGTTTACATATTTTTTT85330.12144942264199819No Hit
ATCCTGAATCGCTGCGACGGATATTGCCTTTTTTT85180.12123592898916448No Hit
GTGCGACAGGGAGTGTATGGTCATCTTGTTTTTTT85100.12112206570765317No Hit
GCGTCTCTGCATTGGGCCCGTATGATTCTTTTTTT84830.12073777713255252No Hit
TCCCGTCAGTCCCGCATCGTGAACACCCTTTTTTT84070.1196560759581951No Hit
TCGGACGCCCAGCCCACCAGGCAACGCATTTTTTT83850.11934295193403902No Hit
AGGGCTGCAGTTACAGGCTGTAAATTTTTTTTTTT83110.11828971658005939No Hit
AACCATGGGATCGCTAAGGTCTATGCCATTTTTTT82740.11776309890306962No Hit
TGTATGGCGCAGACAGTACCACAGATGGTTTTTTT82020.11673832936946785No Hit
GAGATCTGTCACTCCGCACTGGAAGGCGTTTTTTT81880.11653906862682305No Hit
CTCCTCCAGCTCACACCCACATGGTTGCTTTTTTT80760.11494498268566475No Hit
ACTGTCTTCTTTAGAATTAGTTTAAGACTTTTTTT78390.11157178297089226No Hit
CTGAGCAAGTAACAAGTAGAACAACGGCTTTTTTT77800.11073204126974638No Hit
CATGCGTTAGACAGAATAATTTCGTGATTTTTTTT77130.10977843628708918No Hit
CGACACGCTCCGACAGTCTTCTTTGGCTTTTTTTT74470.10599248217683821No Hit
GCGCATCCAGTCAGCACTGGATATTTGTTTTTTTT74180.10557972778135973No Hit
TGTTCGTATTGCGGTGCGGTCTGAGTCTTTTTTTT71930.10237732298885421No Hit
TTCCTCGAGGGTGTCTCTCATATCTACTTTTTTTT71630.1019503356831868No Hit
GAAGCCACTGATTATGAGTCATATATCCTTTTTTT70540.10039894847259523No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)