FastQCFastQC Report
Wed 12 Jul 2023
EGAF00008107290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008107290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12512114
Sequences flagged as poor quality0
Sequence length35
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGCTCCGTGAAGGTAGGTGTTAAATTTTTTT2751812.199316598298257No Hit
TATCCGACCGTCGGGGACAGGCACTCTTTTTTTTT1985361.5867502485990777No Hit
CGCAATTACTTTCGGTGACGGTTCTTAGTTTTTTT1821101.455469475421979No Hit
CACGTTCGTGCTCTAGAACTCTTTTGACTTTTTTT1028720.8221792096843107No Hit
CCAGTAGTCTGATCCATTAGATGGGCCTTTTTTTT1000380.7995291603001698No Hit
GCTGAACTCTCCAGGGCTGCAGCAGCCTTGCCTCT982760.7854468077896349No Hit
TAACCTAGGGAGTCCAATACTATAGCCGTTTTTTT887550.7093525522545591No Hit
CAATGGATCTCTACCAAAGTCTTTATTCTTTTTTT886170.7082496211271733No Hit
ATTGATGAGTCCTAACGGGAGTTGATACTTTTTTT877150.701040607526434No Hit
TGCCTAATTGAAGATTCAGGTATACATGTTTTTTT869660.6950544088712747No Hit
CTAGTTGGGCCCGGTATTGCCATAGGAATTTTTTT835760.6679606659594054No Hit
AAGGATCGATCGCTTGTATCTCCCACCTTTTTTTT808270.6459899582116979No Hit
AGTTCCTATTTATGTTGGGCGAACGGGGTTTTTTT793640.6342972898104988No Hit
GGTATAGTGACACATACTGAGATTTGAGTTTTTTT748010.5978286323158502No Hit
GCTTACGTAGTTAGTATTGCTTTTCGGGTTTTTTT730840.5841059312598974No Hit
TACCGCGGACTTGCAGTCTACAAATCAATTTTTTT721420.5765772274773072No Hit
GTCTAGTGAGCCGCTTAAAAATCGAGCTTTTTTTT720040.5754742963499214No Hit
CCTCACCAATCTTGACGACACGGGGATTTTTTTTT691850.5529441307839746No Hit
AGTGCTTGCACGAATACTGCAGTGGGAGTTTTTTT690650.5519850602384218No Hit
ACGGCACTTGCTTGGGGAGGACGAAAAGTTTTTTT666130.5323880520909576No Hit
GGCGTCCTATCCGCTGAGTACGCTCGTCTTTTTTT660630.5279923120905069No Hit
CTCAGTCACGACAAATTACCCCAGATGGTTTTTTT654530.5231170368172796No Hit
CAAGTGTGGTTGCAAATCGAGAACGATTTTTTTTT647360.5173865903076011No Hit
CTGGGATAAATAATGGTGGAAGGAGACTTTTTTTT646650.5168191402348157No Hit
ACGCGGGCCAAGGACACGAGCCTGGAATTTTTTTT614530.49114801863218316No Hit
GGGAGTTAATGAGGCGGACAACTTTCTTTTTTTTT606830.48499398263155213No Hit
ACGCATTCGTGAGTACGGCGGTGTGGCCTTTTTTT595630.47604265753972513No Hit
GTTGTCGTGTTAGTTGTATATTGCTTTTTTTTTTT595600.4760186807760863No Hit
ACACGTAGGCCACAAGTTAGAGAAGATATTTTTTT584460.46711530921153693No Hit
AACCCAGAGACGGAGATACAGGAGTGTATTTTTTT563760.4505713423007495No Hit
CAAGCACCAAATGCCTCACAGGCCAGTATTTTTTT562000.4491647055006052No Hit
AATTAAAGGTCGGCGTTGTAAAAAATTGTTTTTTT548300.4382153167722097No Hit
ATTATTCAGAGTCACTGATATATGCGCTTTTTTTT533930.42673044698921375No Hit
GTAATAAAGGGCTCCCAGTTTTGTACTTTTTTTTT496790.39704721360435175No Hit
CTTTGCATCGCTCTTGTTTATATGGTAGTTTTTTT488050.3900619831309082No Hit
CGAGCTGGGCTTTAGGAAACGCTCTGGGTTTTTTT485380.387928051167053No Hit
ATCCAGGATTCGTGAATTATCGTAGTGCTTTTTTT481570.38488300218492255No Hit
ACCACCAATGTAACAATAGCTGACTTATTTTTTTT460770.3682591127286724No Hit
CGTTGTAAGATTGATTTTAAAGTGGGCTTTTTTTT457520.36566163000113333No Hit
TCTGAGCAATTGACTGGCCATGCGCGGCTTTTTTT450810.36029882720058337No Hit
CCGGCGCATATTGGATAGATAAGTGACCTTTTTTT448970.35882825236406896No Hit
TACAACGCACAACTCATTGGGTTTAAGATTTTTTT441510.35286603047254844No Hit
CTAATTTCAACAACACTGTTCTCCTGGATTTTTTT434390.3471755452356013No Hit
TCGCCGACATATTCGCGCGTGTTCATGTTTTTTTT431330.34472991534444136No Hit
GGGTCAGGAGCTAGATTGGCTGTTTATGTTTTTTT427190.3414211219622839No Hit
GCAAACGTCGCCAGGTTTGCCAGCCTCTTTTTTTT425360.3399585393803157No Hit
TGCGGAGTAAAGGTGCCGAGGACAGTCTTTTTTTT424090.33894352305293896No Hit
TTGAATATTGACTTTCTCTAAGGTGCTCTTTTTTT421410.3368015988345375No Hit
CCATTCCCTGCCCACAAATCATTAGGGTTTTTTTT421280.33669769952543593No Hit
CACCCTAACAAGATCTTTCTCACATAGGTTTTTTT421000.3364739163981402No Hit
ACGCATTCGTGAGTACGGCCTGAACGAATTTTTTT418910.33480353519796896No Hit
GCCAGGCTTAGTGGTAGTAAAGAAAGGCTTTTTTT415040.3317105326885609No Hit
GGGACTGCATAGATAGTCTTTTACATCCTTTTTTT408370.326379698906196No Hit
CCGCCGTTGAGGATAACGCTAATATTAATTTTTTT408240.32627579959709446No Hit
GCGAGGCCCGAGCAGATATGTCATGGTATTTTTTT400990.3204814150510457No Hit
AGACCGCTCCGCGGTTTTCATGAGACGGTTTTTTT400780.320313577705574No Hit
GCAAACGTCGCCAGGTGAAGAGGACAAATTTTTTT397140.3174043970507302No Hit
CTAGTGAAGGACAGGAACGCGGGCTAATTTTTTTT397030.3173164822507212No Hit
AACCTCGCTTTAGCCCTAACCAAGCGCATTTTTTT393090.31416753395948915No Hit
GAGGAATGGAGAGGTTCTGTTTGAGGTGTTTTTTT392200.3134562233048708No Hit
ATCCAACGCAGTCATAGGAACTTTATAGTTTTTTT388250.3102992827590925No Hit
ACACGTAGGCCACAAGCAGTCATGATAGTTTTTTT373230.2982949164305888No Hit
AGACCGCTCCGCGGTTCCTCATCAGAAGTTTTTTT372870.2980071952669229No Hit
TGCAGAGTACCGAGCACTCCCATATGGATTTTTTT372020.29732785363048964No Hit
CCAAATAACAAGATTCGGGAACATAGTTTTTTTTT371660.29704013246682376No Hit
GCTAAGTAGTTTCTCTGCTCACTTTATATTTTTTT371130.29661654297587126No Hit
CTACTATCTTTCAGAGCCAGTATATTTCTTTTTTT369640.2954256970484764No Hit
CGAGGCTAAATATGGCTAAGGGGTTTGTTTTTTTT368910.2948422624665984No Hit
TTCGGTACTGTAGAGGATATTTCGCACGTTTTTTT361980.28930363006603044No Hit
AGCGACATCCCATTCAAGGACTATTACGTTTTTTT361860.2892077230114751No Hit
CGGCCCAGGTATATCCCGCTTGCCCACTTTTTTTT360010.2877291559204144No Hit
CCAGCTCGAACGCATTGGAACTACTAACTTTTTTT356460.2848919055564871No Hit
ACGGCGACGATGGGAAAAAAATATAGCCTTTTTTT353320.28238233762895704No Hit
CAAGCACCAAATGCCTAGAGGTATGCCTTTTTTTT352260.281535158647052No Hit
AGTACATCATTTATCATGTCCACTGCTTTTTTTTT343360.274422052100868No Hit
AGCAGAAGGAGAAAGATCATAATGCGCATTTTTTT341230.2727197018825116No Hit
AGTTTCGCAGGTCGGACTTTTACCCGTCTTTTTTT340350.2720163834824395No Hit
AAGTGTTTGGAGACGGTACTAGAATGTGTTTTTTT338210.27030604100953687No Hit
TCCGTTAAGCTAATATAGCTCAAGAAGCTTTTTTT330130.26384829933614734No Hit
AGAGGCTTCGGAAACCCGTTTTGGCGACTTTTTTT328400.2624656392996419No Hit
TAGCGTCCGGTGTGGTACGCTCTATCGTTTTTTTT323470.25852545780832875No Hit
GGTGCTGGTACACATTTTTGGTCACACTTTTTTTT323440.25850148104468995No Hit
ACGGATGGTGCGGATATTGTACAGATATTTTTTTT322700.2579100542082657No Hit
CGTAGAGAGTAATTATGCGCCTTTGATTTTTTTTT317990.2541457023169706No Hit
ATTAATACTACGCGGGCCCTGGGATGGGTTTTTTT312590.24982988486198254No Hit
ACCCGGTTACACTTCCGTGTTTACGAGATTTTTTT312380.2496620475165108No Hit
CGCAATTACTTTCGGTGTAATTTGGTATTTTTTTT307320.24561796671609606No Hit
ATAATAGTGTAGGGACGGTGTCGCTTATTTTTTTT306740.24515441595241222No Hit
TTACTAAAGGACTTTACTCGTTCGAAAATTTTTTT304720.24353998053406484No Hit
GTGTATATCAGCGGGCTGATTCAAAGACTTTTTTT304400.24328422838858405No Hit
TAGCGTCCGGTGTGGTAGACGCACAACGTTTTTTT301470.24094249780652577No Hit
TCTGTGCCATCATTAGTTTTCATATTTTCTTTTTT301110.24065477664285986No Hit
TAACTATTACGCCAAAGGCCAGACGAAATTTTTTT297650.237889456569849No Hit
CAGGATATATCGTTGTGTGAAAATTGTTTTTTTTT296210.23673857191518557No Hit
GCAAACCCTACATTATATGATCGGTTGCTTTTTTT294820.23562764853325346No Hit
CCATTAGCGATAATCCAACTCTCACCCTTTTTTTT294070.2350282294422829No Hit
TATTGACATTTCTGCCAGATTCTGTACCTTTTTTT283330.22644454805958453No Hit
AACTACCCGTTTGTCATCCTTCTGCCGGTTTTTTT282630.22588509024134532No Hit
TTGCCTTCTCGCCGGGTACTTAGTTCTGTTTTTTT280230.22396694915023954No Hit
CTAGTAGAAAGGGATTGTTTGTATGGTGTTTTTTT279310.2232316617319823No Hit
AGTCGTATAAAGCAGAATTGTTAGGATTTTTTTTT278860.22287201027739997No Hit
TCCGAATGGTCCTGAGGCAAGCCGAAAGTTTTTTT276800.2212256058408675No Hit
TCAAACAACCGCGTCGCCCCTGTTAGCGTTTTTTT272740.21798075049508023No Hit
TCCACTTTATCTAGGTTTTTGGTATGTTTTTTTTT270430.21613453969489088No Hit
TGAAAGGACCTGACTCTTGAGATTGCTATTTTTTT264230.21117934187620094No Hit
TTATGAATGAAAGGGAGTTAGGATCTGTTTTTTTT262450.20975672056696412No Hit
CCGGTAATGGCTAGTCGTTCTGTAGAGCTTTTTTT262360.2096847902760477No Hit
GATCCGAATATAAGTGATAAATTAGGCATTTTTTT261790.20922923176691005No Hit
TCCGATTACATTGCCGGATTAGGTGCACTTTTTTT261730.20918127823963242No Hit
GGCATACAGGTAGCGGTCAATTTCCAGATTTTTTT260670.20833409925772733No Hit
TCCACTTTATCTAGGTAGTATTAAGCCGTTTTTTT258900.20691947020303683No Hit
TCCCACTCTCTTCCGGGGGGCCTTTCTTTTTTTTT258590.20667171031210235No Hit
GTACTCCTGGGTATGCTCGTGGGTATTCTTTTTTT254430.20334693242085228No Hit
GTAATAAAGGGCTCCCGAACCCTTTAGATTTTTTT252230.201588636420672No Hit
TATTACCATCCTGTTTCGTAATACATCTTTTTTTT251100.20068551165694304No Hit
CTAATTTCAACAACACTGGGTATTTATTTTTTTTT249870.1997024643477513No Hit
AGGGACCGGCTGCGTTTTATATATTTTTTTTTTTT246130.19671336114744478No Hit
TCCCACTCTCTTCCGGTGTTGTCGTTTGTTTTTTT245490.19620185685648325No Hit
AGGTATGCGGACATTACGGGATTGTTTTTTTTTTT241750.19321275365617674No Hit
GTGCGTGTATATGAGCGAAAATACCTGATTTTTTT240530.19223769860153128No Hit
CGAACGCCCAGTGCCGCTTAACGAGGACTTTTTTT239500.19141449638326505No Hit
CCGTACCCAAGCGCCACAGAAGGATGTGTTTTTTT237910.19014372791040746No Hit
CATGCGTTAGACAGAATCGGCGGCTTTCTTTTTTT237390.1897281306740012No Hit
TAACCGCCCGCAGTGCTGAGTGTGTTCTTTTTTTT231840.18529242940081908No Hit
TGTGTCGCGAGTTGCACAGTATATGTGCTTTTTTT229320.183278381255158No Hit
TCGGCTTGTATCGACGCAGTCTTATTCCTTTTTTT229230.18320645096424154No Hit
TACTCTCCGAACAAATCATACTTTACAATTTTTTT228970.1829986523460384No Hit
ATCTCCCACGGAATATAGACTCACTGCATTTTTTT228670.18275888470965018No Hit
TCGAGACCAACACCGTTATGATATAATTTTTTTTT228570.18267896216418744No Hit
CGGGAATTTATGTAAATATCTCCTTAATTTTTTTT228150.18234328747324394No Hit
GTCGGGAAGCAGAAACGTACGCCAGTAATTTTTTT226350.18090468165491458No Hit
TTGAGCAGCCCACGGTGTGCCCTACTGATTTTTTT222640.1779395552182469No Hit
TTGAGAGTACTGCTAATATCATCTGGGGTTTTTTT222340.17769978758185867No Hit
TTAATCAGTACGTCAGGCCTCCTTTTCATTTTTTT221890.17734013612727634No Hit
GCCAACCATTTCCGGAATGTAGGGCAAGTTTTTTT219330.17529411896343017No Hit
TATTGACATTTCTGCCAGTGGTCATATATTTTTTT219070.17508632034522703No Hit
TTGAGAGTACTGCTAATGGTTCGTTAGGTTTTTTT217020.17344790816324085No Hit
TTACCCTAACAGTCCTGCTCACTATGTCTTTCTTT216940.17338397012687065No Hit
CGGAGCAATTTAATCGTAGACTTATGAATTTTTTT215100.1719133952903562No Hit
CATTACGTCGGCCCGTGGTATGGTATTTTTTTTTT213200.1703948669265641No Hit
GGCTGAAATAGCAAAGGTATCCATAACTTTTTTTT212020.1694517808901038No Hit
CCTGTCCCTCACGTTATGCTAGGGAATATTTTTTT211370.16893228434459595No Hit
CCATTTCTACCTATTATCGCCTATAGGTTTTTTTT209740.16762954685355327No Hit
AAGTCAATTGTCGTCAAGTTTTCCCAGTTTTTTTT209060.16708607354440663No Hit
TCCGCCTGTCTACAAGTGTTCCCAATGTTTTTTTT208790.16687028267165724No Hit
AGTAGGTAACATACATGCAAAATGAGAGTTTTTTT208490.16663051503526902No Hit
GACAACGCAGCTTACGGCATGAGTTTTTTTTTTTT208190.1663907473988808No Hit
ACGATCATACATAGAGCGTAGCTATTGTTTTTTTT207830.16610302623521492No Hit
CGCAGGCGATCCAAACGACTAGACGGGGTTTTTTT207240.16563148321698476No Hit
CACACAGCTTGCGCTCCCCCGCAACACGTTTTTTT205920.1645765056168766No Hit
CCACCCAAGGAAAGTGAAATGACATCTATTTTTTT205310.16408897808955386No Hit
AATCGTGAGCCGAGCATGTCGATTGTATTTTTTTT204200.16320183783491743No Hit
AGAGTAAACTTCACTATCGCCTATCGTGTTTTTTT202140.16155543339838496No Hit
TATCCTGCATGGGAATAACCTTTTATCCTTTTTTT199530.15946945496180742No Hit
GCGAAACGATCGGGAGTTTAAGAACTTTTTTTTTT198730.1588300745981055No Hit
TGAATACCGACGCGTATATTACCTGCTGTTTTTTT198150.1583665238344216No Hit
GATTGGGAAAGGTTGTAAGCGCGGCCTATTTTTTT195770.15646436725240834No Hit
CTTCCGCTCCGTGAAGGTAGGTGTTAATTTTTTTT193510.1546581177249504No Hit
ATTACATGTCAGTCTTTTTGAAGGACCTTTTTTTT193260.15445831136129354No Hit
CTTCAACTCCACTTGGTATCTAGAAAGATTTTTTT192170.15358715561574968No Hit
GATTGGGAAAGGTTGTATCTCTGCGTTATTTTTTT187040.14948712903351105No Hit
GACCGACGTGAAAGCACAGCTATACTTGTTTTTTT186580.14911948532438243No Hit
CTTTAGGAACACTATTAAAAAAAAATAAACCCCTC185470.148232345069746No Hit
AGCGGGAAGGGTCCATATCCAGGTGTTGTTTTTTT184810.1477048562696919No Hit
TAGAAAGGTGGCGCTATTAGTCTGCCAGTTTTTTT183620.1467537779786853No Hit
TATTACCATCCTGCTTAAGCACCAGTAATTTTTTT183030.14628223496045512No Hit
TGCACCCATCTGCGGAACGGATCTAGCTTTTTTTT181930.14540308696036497No Hit
CCCAGTTAAGGCGCCGGAGGCGTGTTTATTTTTTT181740.14525123412398575No Hit
TCGTAAGACGACATTGCTCTTCCCCTGTTTTTTTT181410.14498748972395872No Hit
TTGCTGATCATGTTCGTAAGTAAGCCTTTTTTTTT181080.14472374532393167No Hit
TGCGGTGAAATTTCATGTGACGTTTTCTTTTTTTT179730.14364479096018468No Hit
AATCGTGAGCCGAGCAATAGACCCCGTTTTTTTTT179480.1434449845965278No Hit
GACACTGGAACCCGATAGTAATAGCAATTTTTTTT178220.14243796052369725No Hit
TCCGCCTGTCTACAAGCCACTTCAATTTTTTTTTT178200.1424219760146047No Hit
GCTAAGTAGTTTCTCTTGGCGTATAATCTTTTTTT177560.14191047172364318No Hit
CCTTTCAATGAAGAAAAAGACAAGAAACTTTTTTT177500.14186251819636556No Hit
CCAATGTCACAGCAAGCTATTTCTTATTTTTTTTT177330.14172664986907887No Hit
GAATGCGAATCGGTTCGTCAACTGGCCTTTTTTTT175830.14052781168713777No Hit
CTCAGGACTCACCTGTTTGCAACAGATTTTTTTTT172120.13756268525047008No Hit
AGGATAAAGTCGGGATTGCTATCGGCGTTTTTTTT172080.13753071623228497No Hit
CTGTTCACTGCCTGTGGGTTGAATTCGATTTTTTT169110.13515701663204155No Hit
AAGGCTGTGCTCATCGTATGTGGCTGGTTTTATTT168250.134469682741062No Hit
TCTTACGGCATCCGACGTTTATAGCTAATTTTTTT167290.13370242630461965No Hit
AAGAGGCCCTTTGGAATCAGCGTACTCTTTTTTTT166890.13338273612276869No Hit
GCGACATGTAAACATCGCTGTAGGCGTGTTTTTTT166380.13297513114090873No Hit
ATATTGCTGTCAAAGTGATGACTTTCTGTTTTTTT166210.13283926281362207No Hit
TTACCCTAGGGATTGGATAGCTAGCCGCTTTTTTT164990.13186420775897661No Hit
CACACACGCTAACGTGTAAGATCCTGTGTTTTTTT163870.13096907524979393No Hit
TTGACCAGGAACAACTTGTTAATTCAGGTTTTTTT163170.13040961743155471No Hit
GCTTAATGTAACTAACTTCAGCCTGTGATTTTTTT160590.128347615758616No Hit
ATCTCCCTGCAATCTATACTTGTCCCAGTTTTTTT160100.12795599528584856No Hit
TCAAACTTAGATTGTTGCTCAGTCCTACTTTTTTT158470.12665325779480588No Hit
ATACCTAACCAAGAAATAATCACGTTTCTTTTTTT152280.12170605223066222No Hit
GAACGCGGGTCACACGATATGTCCACGTTTTTTTT151960.12145030008518146No Hit
AGGATAAAGTCGGGATTGCTATCGGCGTGTTTTTT151650.12120254019424696No Hit
CCTATGAAGTGGTGCCGTAATCACAAACTTTTTTT149800.11972397310318623No Hit
TGGGAAATGCCTTTCCGCTTTACACGTCTTTTTTT148970.11906061597584548No Hit
TCAATACGCCGTCATGGATCTGGTATTTTTTTTTT142400.1138097047389434No Hit
AACCTCGCTTTAGCCCTTCTTGGAGGCTTTTTTTT141930.1134340687752685No Hit
AAATTCCAGGTCCAAACAGTTAAACTATTTTTTTT141470.1130664250661399No Hit
AAATTAACGGGTAGCTAACGAAAAGCTTTTTTTTT139160.11122021426595058No Hit
AAGAGGCCCTTTGGAAGATCAGTACCGATTTTTTT138440.11064477193861884No Hit
CGGCTCTAAAGCTGCATGAACCCGACATTTTTTTT137460.10986153099308398No Hit
CCCTCCTCGCTCGTATAAATTATTTGCGTTTTTTT133830.10696034259278647No Hit
CACCTTGGAAACTCGCGACTGTACTAGTTTTTTTT133520.10671258270185198No Hit
AGAGCGGGCTAATCATTTATTTCATATTTTTTTTT133290.10652876084728767No Hit
ATTTAACTCGTATTACACGCCATCTGTATTTTTTT132450.10585741146540065No Hit
AACTGGGTCCCGACGTTTTTATCCACAATTTTTTT131410.10502621699258814No Hit
TATGGCCCGGCCTCGCGGCCTTCGACACTTTTTTT131340.10497027121076422No Hit
CACCTTGCGAAACTCGCGCTGTTTTTGTTTTTTTT131180.10484239513802385No Hit
AACAGGATGGGCCGCGTGGGTCTAATTTTTTTTTT131070.10475448033801484No Hit
CGATTCGCCTGGCTGCCGACCTTGGACATTTTTTT129830.10376344077427684No Hit
GATCTTGGAGGGCATAGTACCTCAAAGGTTTTTTT129560.10354764990152743No Hit
CCTGAACGATATATTCGTTTGTGCTGCATTTTTTT126930.10144568695585733No Hit
TGCGGTGAAATTTCATGAAATTATCTACTTTTTTT125890.10061449248304484No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)