FastQCFastQC Report
Wed 12 Jul 2023
EGAF00008107292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008107292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36425258
Sequences flagged as poor quality0
Sequence length35
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGCCTATATCACATATTGTATCTCGCTTTTTTT2512870.6898701994094317No Hit
GACCGTGCTGACGGTGAGTGCGATGGTTTTTTTTT1892260.5194911728559343No Hit
CCACGAATTTAACCTCTATATGTGAGCTTTTTTTT1453950.3991598357381573No Hit
CTAGTGAAGGACAGGATTGACCGTATTTTTTTTTT1247470.34247389544914136No Hit
AATTCCAAGCATGTACTGAGTCGAGGGATTTTTTT1056460.2900350081254057No Hit
CATCGCCCGCGGCCAACACTACTTCGTTTTTTTTT1046440.28728416968247694No Hit
CATGCTGGCTCCAATTTACTGCAGTTGGTTTTTTT1017990.27947365534102736No Hit
TGGCAATGGGACGGCGCGAGAGGAAGTCTTTTTTT952520.26149986363857736No Hit
CTCGAGGTCGAACAGTTCGCCTATAGAATTTTTTT946640.25988559916308623No Hit
ACACTGGGACAGTCGTACGGAAATTATTTTTTTTT909010.2495548555894923No Hit
CGGCAAACATCGTGCGATATTCACCTGTTTTTTTT893130.24519524336656723No Hit
CTTAGTAGGCCTACAGCACGAGTTTGTTTTTTTTT884050.24270246761189723No Hit
CAGCAGCCCGTTCCTTTCGGTCTGATCATTTTTTT864540.2373462941566536No Hit
CATGGCAGGAAGATCGTTCGGGTGCTTGTTTTTTT856760.23521041360915No Hit
CATGATGCACAATTCTGCGTTACCACAGTTTTTTT854670.2346366359299363No Hit
CTGCAAGCACGTTCCGTTTCTTCTTAAGTTTTTTT837870.2300244517142473No Hit
ACTATCTGCCCGCGTACTGCAGTCTGCGTTTTTTT804220.2207863565441321No Hit
ACCACAAGTTTCTATCTAATCGTCGCTATTTTTTT783770.21517212040062966No Hit
GACAACGCAGCTTACGCGGCGGTTCCGGTTTTTTT769200.21117214873261847No Hit
GCAAACCTTGGCCATAGGACTCTGGTGATTTTTTT760950.20890723684098547No Hit
TCAGAACCTCCACAGGTGAGTTTGGGCATTTTTTT747770.20528886850986755No Hit
CTGGGATACGCTACCCTGCGGACATTACTTTTTTT738290.20268627884530016No Hit
TTATCCAATCGAACTCAGTCGTAAAGTATTTTTTT733720.20143165492472284No Hit
TCTCTCGCCGCACATAATGCACTCTGTTTTTTTTT708750.1945765215993803No Hit
ACCAACCGCACTCCACGTCTTATCAGCAAGATCTC677010.185862787849025No Hit
TCAGTGTATACGTCATCATTGTACCTTTTTTTTTT674150.18507761839325887No Hit
CCAAGCGTAACTCGTAGCACCGCAACCATTTTTTT659450.181041957204531No Hit
TCGGGCCGTCGTGGTATTATGCGTTGTATTTTTTT650140.17848603845167No Hit
CGGCCCAACCTGTAGTAGCTTTAGAGCCTTTTTTT641270.1760509149996961No Hit
TCGGAGTACATGAGTAACACTATTGGTATTTTTTT637610.17504611772413528No Hit
GATTGGGAAAGGTTGTGTGTCCCGTGACTTTTTTT634720.17425271222512687No Hit
GAGGCCTGTTGATACAATGGTGTAGGTCTTTTTTT625820.17180935272991066No Hit
GTAACATCTAAGATAAGTTCCGAGTTGGTTTTTTT619200.1699919325211094No Hit
TGGTTCGTAGCAAAGGATAACTTTCATGTTTTTTT616380.16921774445633303No Hit
ATCTGTAATTGTACCCATCGAGCATGTATTTTTTT610250.167534846287156No Hit
CCTATGAAGTGGTGCCCTTATTGAGTAATTTTTTT599000.16444633007129283No Hit
GAATAGCATTTAGGGTTTTCTTGGGAGCTTTTTTT594700.16326583053989624No Hit
CCGGCACGACCGTTTCACGTGTATCTCCTTTTTTT594620.16324386775791677No Hit
GAAATGGCGGTGTTAGGTCAAGGGGATCTTTTTTT588150.16146762776532703No Hit
CGCCCAGCGTTTCACGTCTGCTTTAGAATTTTTTT576870.15837087550622153No Hit
CACATATTAGCAGGATCAACGCCGTTCCTTTTTTT576250.15820066394588064No Hit
CTCGCCGAATGTAGGGAGAGGTAGGCTTTTTTTTT565790.15532903020206473No Hit
GTTCATCGTTTGGCTGATTTCGGTAGGTTTTTTTT564440.15495840825616114No Hit
CTGTGCAGGGTAGGTCCACGTCATGTTTTTTTTTT560640.15391517611213623No Hit
ACAGGCACGGATCCTTCAGAGTTAACTTTTTTTTT559950.15372574711756332No Hit
CCTATGAAGTGGTGCCTGTTGATTCTGTTTTTTTT559160.15350886464551602No Hit
AAACCTAAGCAGCCGGTAATGGGCCACCTTTTTTT554500.15222953259521183No Hit
CTTACACTGGGAAATATTGGTCAACGCGTTTTTTT552620.15171340721869425No Hit
GCATTCAAGGCAACGCTTTGCTGAGCTGTTTTTTT546370.14999756487654803No Hit
TGACCCACGTTAGACAAAGTGAGGCTCTTTTTTTT542030.1488060839541617No Hit
CGGGATCAATGTAAGAAGGAATAGTGCCTTTTTTT530110.14553362943922044No Hit
GTGTACCTTGGCTACGTAGTGGTGTGCATTTTTTT518670.142392951616156No Hit
CCATCCATACCAAGTCAAGGCTACGGCGTTTTTTT515670.14156934729192583No Hit
GATCCGGGAATTAACACGACTCTATCGATTTTTTT514690.1413003032126773No Hit
AACCTGTCACGGAATTGTCACATGAATATTTTTTT513240.1409022277892994No Hit
AACGATAATGCCGTAGTATTTTTTTGCGTTTTTTT503300.13817335212835005No Hit
TAAAGAGCCCGAAACCAAAGGGTCCACTTTTTTTT503160.138134917259886No Hit
GCTATCGCGGCGCAACTCTGAGTCTAGTTTTTTTT497060.13646025513395127No Hit
TCGTCTTAGGCGTTAAAAGGTCATAAATTTTTTTT496590.1363312237898219No Hit
TCCGGCCTAGCGTACATAGATACGCTTTTTTTTTT493620.135515855508834No Hit
CTCTAGCCCTCGGAAAGATTTTCACGTTTTTTTTT492720.13526877421156494No Hit
TCGGGAACGTGCCTAGATCATATCCGTATTTTTTT486120.13345684469825855No Hit
CTCGGTACCACTGCTCACTCGAGTCTTTTTTTTTT485890.13339370170006756No Hit
ATAAAGGCTCGGTCGTCATTCTTGAAGGTTTTTTT484590.13303680649290114No Hit
TTAGTAGGGCGGCGGGTGCGGTGGTCGGTTTTTTT482930.13258107876682712No Hit
CGCAATTAGGGTAATACGTCGCCCAAGTTTTTTTT482350.13242184859747597No Hit
AAGGATGAGGACCTCGCAGATAGCTATTTTTTTTT480780.13199082900112882No Hit
ACTGTCCAGGATTATAGTTGATACCGGATTTTTTT479930.13175747444259694No Hit
AGCCCTAAGCGAAGTTATAAAGTAGGGTTTTTTTT479740.13170531283539572No Hit
GAGCACGGCGCCTCTTGATTGATTTCGGTTTTTTT478680.1314143059741677No Hit
GGATCCGGAATATACTTAAATTGGTGGATTTTTTT478180.13127703858679599No Hit
GCGAAGCCATACCCGTATTCTCTACTTGTTTTTTT477490.13108760959222307No Hit
TAGTGCCCTCCAGAGTTACAGCCTGCGGTTTTTTT474570.13028596804997236No Hit
TCGCCGACATATTCGCGGTTGTTGCGACTTTTTTT474420.13024478783376087No Hit
GTTCTTCCCTCGATGTGCATATCGTTTGTTTTTTT471570.12946236372574216No Hit
CTTCATAGCTCAAGAAAGTTTTCAGAGGTTTTTTT466110.12796340385564325No Hit
GAAATTCACATCGCTGACAGAGAGCCTTTTTTTTT465980.1279277143349266No Hit
GCCAATAGGGCATCTCGACGGTTGCTTTTTTTTTT461330.12665112763236983No Hit
TAGTGCCCTCCAGAGTGGGCCTTCCCTGTTTTTTT460040.12629697777295085No Hit
AGGACATCGCACGTCGGCAACCTTTTGATTTTTTT458890.12598126278199592No Hit
TTGACCAGGAACAACTAAGGCGTTTCGCTTTTTTT457790.1256792745297782No Hit
CCGAGAAGTCGCATAACGTGAATGTGGGTTTTTTT455370.12501490037489918No Hit
AGCAGCCAGATGAATACATTAGTAAGCTTTTTTTT452700.12428189252633433No Hit
TCGCTTTAAACGTTTGTAGGCGATCCTTTTTTTTT450380.12364497184892967No Hit
GGGTTCATATGAAAGCGTATTATCAGGTTTTTTTT446900.12268959083282266No Hit
CGCAATTCTACAATAATATATGTGTAGGTTTTTTT445580.12232720493016136No Hit
GTTATAATACGGTGAAGTCAGATCCACGTTTTTTT444720.12209110502388205No Hit
GCTCCATGAGTGCAGACGTTTGTAGGCCTTTTTTT444140.12193187485453089No Hit
TCTACCCAATAGAGAGACAGCTTTAAATTTTTTTT441810.12129220882937879No Hit
CCATACTTTACTTGTACAATATTTCATTTTTTTTT441600.12123455652668268No Hit
CACAGTTCGCTTCCCAAGATTTTAATAATTTTTTT440680.12098198453391876No Hit
TCCCGCGTACTCCTGGATAGATGCTAGTTTTTTTT440200.12085020784204192No Hit
TGTATCCTTATTCCATGTCGTATGATTTTTTTTTT439700.12071294045467022No Hit
GTGATCACTAACGCCTGGCTCTTCCTTTTTTTTTT439170.12056743702405623No Hit
TAACCTAGGGAGTCCAATTTCTTCTTAATTTTTTT439110.12055096493757161No Hit
CATGCGTTAGACAGAATTTATTAACGAATTTTTTT438300.12032859177002946No Hit
TCTGATCGGGTGCTAGTAGTTTTGCGCTTTTTTTT435200.11947753396832496No Hit
TTCTGCGGGTTAGCGGCTCAGTTCTTATTTTTTTT434760.11935673866743784No Hit
AATGACTGTCAGCCGGCGTAATGCCCTTTTTTTTT432810.11882139585668823No Hit
GCCGTGGAAGAAATGTTCCACGCGAGGGTTTTTTT432620.11876923424948699No Hit
TGGAAGGATAAAGATGCATCTTTCGGACTTTTTTT432070.11861824012337813No Hit
GCGCTTAAATAATTGGAGTAAAAAACATTTTTTTT431660.11850568086573332No Hit
TGAGTGCCTCTTAAATTAATTTACGACGTTTTTTT429910.1180252450099324No Hit
TTACATCGTGGCCTGGTCGGCTAGTAGTTTTTTTT428580.11766011375952369No Hit
ACCCAACGCCCGTGGCAGAGGTATTAATTTTTTTT426690.11714124303525866No Hit
TTGCCAAGCAGAACCCTGGTGATAATAGTTTTTTT424870.11664158974522569No Hit
CGACTTTGTACAGCCTTACGTGCCTATCTTTTTTT424370.11650432235785399No Hit
GTACTGAGGTCGTAACTTCACCACTAGCTTTTTTT424280.11647961422812708No Hit
AATATCGAATCAATGCTCACTTCCATCTTTTTTTT423980.11639725379570408No Hit
ACGCATTGTGAGTACCAGCGATATATTTTTTTTTT421710.11577405985703655No Hit
GCTTTATTAAGTTACCATTGCGCATAGTTTTTTTT421500.11571640755434044No Hit
TCTCGACGTATCGCCGAAAGTAACAACTTTTTTTT420660.115485798343556No Hit
GTTCGCTGAGACGTCTATTCGCGCTCGGTTTTTTT420480.11543638208410219No Hit
AGGCAGATGCGTAAACGATTCTCTGTCTTTTTTTT419410.11514262987512676No Hit
GCTATACGTCTCGGACTAAGGTAGTTTATTTTTTT418780.11496967296703842No Hit
GTGACCGCACACTACGCTTAGATGATATTTTTTTT418750.11496143692379611No Hit
ATTATGCCATAGGGAGCTCATTTTAGTCTTTTTTT418610.11492300205533205No Hit
CACAGTTCGCTTCCCACCATGATACAAATTTTTTT417620.11465121262833608No Hit
GAAGCGTGAGGAATTTTTTTTGCGTCCGTTTTTTT414580.11381662691311617No Hit
ACCGATGGTAGCATCGTCATCAACATTGTTTTTTT414060.11367386883024959No Hit
ATTGGATTACAGCGTATATGCGTGAATTTTTTTTT413450.11350640261765614No Hit
ACTTGGGACCCGGTGGGAGGGATTCAGCTTTTTTT413250.11345149566270746No Hit
ATTACTAGCCTCTTGCACGTGATGACATTTTTTTT412960.11337188057803187No Hit
CTGCGGTGAAATGTTTAGCTGTATTTTTTTTTTTT412150.11314950741048974No Hit
CAATGGATCTCTACCAAATTCCGGAATGTTTTTTT411590.11299576793663342No Hit
TCGGAGTACATGAGTACACAATATGTCCTTTTTTT411030.11284202846277712No Hit
CCACTATCCGGGTCACACAGACACTTCGTTTTTTT411020.11283928311502968No Hit
CATGCGTTAGACAGAAAGATTTGCGTCTTTTTTTT408630.11218314500339298No Hit
TGTACGAACAAATCCGTAGGTTATTTTATTTTTTT406740.11166427427912796No Hit
GAAGCGTGAGGAATTTTATTTCGTTGCTTTTTTTT405390.11129365233322437No Hit
ATATCGGTAGGGAGATCTATACATGGCTTTTTTTT404890.11115638494585268No Hit
GGAGCAACATTTCAAGTTCAGTAGATTATTTTTTT403260.11070889326302095No Hit
CTAGTTACTACCCGGTCCTGTATAATTGTTTTTTT399670.10972331342169216No Hit
CGATACCTCGCGGACATCGAATTTGTGCTTTTTTT399550.10969036924872297No Hit
ATGTAAGGCTGCTCTTGCTACATTAAGATTTTTTT398750.10947074142892825No Hit
TTGTTCAGTGTGCTACGCTACTATTACTTTTTTTT398620.10943505190821161No Hit
ACTTGGGACCCGGTGGCCTTCACGTCTTTTTTTTT397880.10923189617490149No Hit
AACGATAATGCCGTAGCATGAAGAGTGGTTTTTTT396450.10883931144701844No Hit
TTAATCAGTACGTCAGGACCGCGTACCATTTTTTT395310.10852634180381097No Hit
CATGGCAGGAAGATCGGTGGAACGGGGTTTTTTTT394500.10830396863626883No Hit
CTGGGATACGCTACCCCTCTAGTCTTGGTTTTTTT393460.1080184524705357No Hit
TGGACTGTTCGCTCAAATTCCTACCATTTTTTTTT392230.10768077469760132No Hit
TCGCCGACATATTCGCGTACATATGTATTTTTTTT390820.10729368066521314No Hit
TAGACGAACGCCAATTGAATGGCCATTTTTTTTTT389450.10691756802381468No Hit
AGATCGTGCATAAGATTTGCCTCTATCCTTTTTTT387910.10649478447070987No Hit
TACCTCACGCTTGTACTTGTGTGAGACGTTTTTTT387810.1064673309932355No Hit
ATGTAAGGCTGCTCTTCAGATCGCATCTTTTTTTT387510.1063849705608125No Hit
GGGCTGCCTAGGGCGATGTGGCGTTATCTTTTTTT387440.10636575312658045No Hit
ATATGTCTCCCTAGCCCCTCTCAATAAGTTTTTTT386990.10624221247794592No Hit
AGCAGAAGGAGAAAGATTACTATTCACGTTTTTTT384470.10555038484559259No Hit
GAAACAGCCATGCAGTATATCGACAAAGTTTTTTT384380.10552567671586567No Hit
AAGTCAATTGTCGTCACATCTGGATAAATTTTTTT382400.10498209786187376No Hit
CGAACAGTATGGGCGTACATGTGTATCCTTTTTTT382380.1049766071663789No Hit
ACTCAATAAAGGCACGGTTGCCATTCTGTTTTTTT382030.1048805199952187No Hit
CTAGATGTGAGTGTAAAGATTGATATCATTTTTTT380740.10452637013579974No Hit
TTCGACAGAGCCCGTGTTCTTGGGGTAATTTTTTT379710.10424359931781402No Hit
CTTCGTAGATAGGTGACACCGCGCCCGTTTTTTTT379220.10410907727818977No Hit
GATCGGTGGCCATAACGATCTAGTGACCTTTTTTT378310.10385925063317326No Hit
GACCGACTGAAGCGTCTGAGTTTCTGTTTTTTTTT377480.10363138677013627No Hit
ATCGCACGCCGGGAGATGTGCTTTCGGCTTTTTTT375470.10307957187290205No Hit
TATACGCGTCATCACTAGGCCCAACGGTTTTTTTT374430.10279405570716892No Hit
AGACGAAGTGCCGGTCATAACCTGCAACTTTTTTT373150.10244265119549736No Hit
GAAAGTGACTAACTGCTACAAAAAGATATTTTTTT373060.10241794306577047No Hit
GTGACAGCTTCCCACTCAGACTCACGGTTTTTTTT372280.1022038059414706No Hit
AACCCGAGCAGAATCGTTGTAGATCACTTTTTTTT370330.101668463130721No Hit
AAGTGCCTTGACTGTAATTCTAGCGAGATTTTTTT369550.10145432600642114No Hit
GGTTTAGCCTTTCTTGGAGATCTTCATTTTTTTTT369060.10131980396679689No Hit
CCACAGTACCCATCCTAGCTTATATTGTTTTTTTT369050.10131705861904944No Hit
TTGCACGGAGCAGCACGGGCGTCTAGAGTTTTTTT367500.10089152971819719No Hit
TCCCGTCAGTCCCGCATGTTCCGTCCGGTTTTTTT366870.10071857281010886No Hit
GCTAATACCGAATGCCTTCCTTAATAACTTTTTTT366800.1006993553758768No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)