FastQCFastQC Report
Wed 12 Jul 2023
EGAF00008107299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008107299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11434020
Sequences flagged as poor quality0
Sequence length259
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGAATGTGAGCACCTTGGAGCTGGGGGACTCGGCCCTTTATCTTTGC1842901.611769089086778No Hit
CTGAATGTGAACGCCTTGGAGCTGGACGACTCGGCCCTGTATCTCTGTGC1470771.286310501468425No Hit
GAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTGTATCTCTG1419321.2413132039300263No Hit
GAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTCTATCTCTG1169201.0225624933313042No Hit
TCTACTCTGAAGATCCAGCGCACAGAGCGGGGGGACTCAGCCGTGTATCT1020710.8926956573453606No Hit
AGTTGGCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTTAC909040.7950309689855362No Hit
AGTCAGCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTTAC901490.7884278670144009No Hit
CAAGATCCAGCCTGCAAAGCTTGAGGACTCGGCCGTGTATCTCTGTGCCA801820.7012581751649901No Hit
GGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAGAGCAGGGG737510.6450137396995982No Hit
AGGATCCAGCAGGTAGTGCGAGGAGATTCGGCAGCTTATTTCTGTGCCAG700440.6125929463128453No Hit
CTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGAC681320.5958709185395862No Hit
ATAGCTCTGAGCTGAATGTGAACGCCTTGGAGCTGGACGACTCGGCCCTG632260.5529638744728451No Hit
GATTTCCTCCTCACTCTGGAGTCCGCTACCAGCTCCCAGACATCTGTGTA586090.5125843753990285No Hit
AGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTGTATCTCTGT527600.4614300132411872No Hit
CATTCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTG498520.43599713836428483No Hit
CATCATTCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCT483970.42327195509540827No Hit
AGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTCTATCTCTGT440050.38486026786729427No Hit
GAACTGAACATGAGCTCCTTGGAGCTGGGGGACTCAGCCCTGTACTTCTG431940.3777673993923397No Hit
GCCAGAAGACTCGGCCCTGTATCTCTGCGCCAGCAGCAACGACAGGGGCG420700.36793708599425223No Hit
AACTATAGCTCTGAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGC376960.3296828237137945No Hit
AACTATAGCTCTGAGCTGAATGTGAACGCCTTGGAGCTGGACGACTCGGC375200.3281435575589338No Hit
TATTCCTTCACCTACACACCCTGCAGCCAGAAGACTCGGCCCTGTATCTC359860.314727453686455No Hit
ATAGCTCTGAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTG314220.27481148362518165No Hit
GGGACTCAGCTTTGTATTTCTGTGCCAGCAGCCTCGCCTCGGAGTCAGGC305080.26681779461641664No Hit
GGGACTCAGCTTTGTATTTCTGTGCCAGCAGCGTAGGGGACACTGAAGCT293890.2570312103704559No Hit
ATAGCTCTGAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTC262800.22984042357805917No Hit
TTCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCG255170.22316735496352114No Hit
TCTACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCCGCCGTGTATCT225800.19748085100428373No Hit
GCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTTACGGGCA204850.17915833626318653No Hit
AGCTGAATGTGAACGCCTTGTTGCTAGGGGACTCGGCCCTCTATCTCTGT193030.1688207647004291No Hit
GCCAGAAGACTCGGCCCTGTATCTCTGTGCCAGCAGCCAAGAGGGGGGAG192190.1680861149447001No Hit
ACCAGACCTCTCTGTACTTCTGTGCCAGCTGGAAGTGGACTAGTGAAGCT190720.16680047787217445No Hit
GCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGCCTCGGAGG186120.16277739587651588No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG184240.16113317975655106No Hit
AGTCAGCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTGAA183450.16044225915294882No Hit
GCTACTCTGAAGATCCAGCGCACAGAGCGGGGGGACTCAGCCGTGTATCT173550.15178388703185755No Hit
GCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGCTCGACAGG172900.15121540805421016No Hit
AGTTGGCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTGAA172480.15084808317634568No Hit
AGCTGAATGTGAACGCCTTGTTGCTAGGGGACTCGGCCCTGTATCTCTGT152710.13355757642543917No Hit
TTCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATTCAGCG148150.12956947775148198No Hit
CTGAATGTGAACGCCTTGGAGCTGGGGGACTCGGCCCTTTATCTTTGCGC140960.12328122567565912No Hit
CTGAATGTGAACGCCTTGGAGCTGGAGGACTCGGCCCTGTATCTCTGTGC140620.1229838674411974No Hit
GCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGCCTCGGAGGGGT139570.1220655552465362No Hit
GCCAGAAGACTCGGCCCTGTATCTCTGTGCCAGCAGCTTGAGACTAGCGG138650.12126093884740449No Hit
GATTCCTTCACCTACACACCCTGCAGCCAGAAGACTCGGCCCTGTATCTC137780.1205000516003995No Hit
GCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTGATCAGGG130650.11426427450712874No Hit
GCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGCTCGACAGGGGC129690.11342467478629563No Hit
GCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGCCGGACAGG129040.11285619580864822No Hit
GCCAGAAGACTCGGCCCTGTATCTCTGTGCCAGCAGCCAGCAGGGTGCAC126010.11020620918976878No Hit
GCCAGAAGACTCGGCCCTGTATCTCTGTGCCAGCAGCCAAGATGCTATTC123510.10801975158343259No Hit
GCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGCGAGAGGGT122830.10742503511450917No Hit
GCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTTACTCGGC119910.1048712526303085No Hit
GCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTTACGGGCAGAA118010.103209544849493No Hit
AGTTGGCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGCTCG117540.1027984908195018No Hit
AGTCAGCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTTCC114690.10030592914827856No Hit
GCAGGAGGACTCGGCCGTGTATCTCTGTGCCAGCAGCTTAGGCACAGGGG114470.10011352087892096No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)