FastQCFastQC Report
Wed 12 Jul 2023
EGAF00008107301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008107301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7524034
Sequences flagged as poor quality0
Sequence length259
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAATGTGAACGCCTTGGAGCTGGACGACTCGGCCCTGTATCTCTGTGC1089461.4479732547726392No Hit
AGATGAATGTGAGCACCTTGGAGCTGGGGGACTCGGCCCTTTATCTTTGC1064491.4147862702374816No Hit
GAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTGTATCTCTG843831.1215127417021242No Hit
GAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTCTATCTCTG714160.949171681042377No Hit
TCTACTCTGAAGATCCAGCGCACAGAGCGGGGGGACTCAGCCGTGTATCT552780.7346856752640937No Hit
GGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAGAGCAGGGG511090.6792765689256588No Hit
AGGATCCAGCAGGTAGTGCGAGGAGATTCGGCAGCTTATTTCTGTGCCAG508230.6754754165119402No Hit
AGTCAGCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTTAC481420.6398429353190058No Hit
AGTTGGCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTTAC476400.6331709824809404No Hit
ATAGCTCTGAGCTGAATGTGAACGCCTTGGAGCTGGACGACTCGGCCCTG449290.5971397790068467No Hit
GAACTGAACATGAGCTCCTTGGAGCTGGGGGACTCAGCCCTGTACTTCTG349390.46436525938080553No Hit
GGGACTCAGCTTTGTATTTCTGTGCCAGCAGCCTCGCCTCGGAGTCAGGC345660.4594078123517252No Hit
CAAGATCCAGCCTGCAAAGCTTGAGGACTCGGCCGTGTATCTCTGTGCCA331860.44106658741839816No Hit
AGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTGTATCTCTGT320860.4264467704425578No Hit
GATTTCCTCCTCACTCTGGAGTCCGCTACCAGCTCCCAGACATCTGTGTA319000.42397469229937024No Hit
AGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTCTATCTCTGT277730.3691237971545583No Hit
AACTATAGCTCTGAGCTGAATGTGAACGCCTTGGAGCTGGACGACTCGGC272010.36152149232712133No Hit
CATTCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTG252850.33605642930374846No Hit
CATCATTCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCT249460.3315508675266486No Hit
CTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGAC242170.321861916094478No Hit
TTCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCG238270.31667852643940736No Hit
GCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGCTCGACAGG237210.31526970771264456No Hit
AACTATAGCTCTGAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGC225520.29973282949013785No Hit
GGGACTCAGCTTTGTATTTCTGTGCCAGCAGCGTAGGGGACACTGAAGCT224380.2982176848217326No Hit
AGTCAGCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTGAA211920.28165741941091704No Hit
TATTCCTTCACCTACACACCCTGCAGCCAGAAGACTCGGCCCTGTATCTC209220.2780689188804835No Hit
AGTTGGCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTGAA198570.26391427789932903No Hit
ATAGCTCTGAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTG184840.24566608816493918No Hit
GCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGCTCGACAGGGGC177100.2353790533110297No Hit
GCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTAAGACGGG171210.22755080585760246No Hit
AGTTGGCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGCTCG162680.2162138023299735No Hit
ATAGCTCTGAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTC154700.2056077896511366No Hit
GCCAGAAGACTCGGCCCTGTATCTCTGTGCCAGCAGCTTGAGACTAGCGG146310.19445685652138203No Hit
GCCAGAAGACTCGGCCCTGTATCTCTGCGCCAGCAGCAACGACAGGGGCG138210.1836913549300814No Hit
GCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTAAGACGGGACT129360.17192904763588257No Hit
AGTCAGCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGCTCG128600.17091895119027903No Hit
GCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGCCTCGGAGG125960.16741019511607735No Hit
GCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTGATCAGGG112190.1491088424108663No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG110190.14645069386980442No Hit
AGCTGAATGTGAACGCCTTGTTGCTAGGGGACTCGGCCCTCTATCTCTGT109840.1459855178751186No Hit
TCAGGGGACACAGCACTGTATTTCTGTGCCAGCAGCAAGAGCGGGACAGA108970.14482922325975667No Hit
GCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGCCGGACAGG106070.14097490787521694No Hit
GCCAGAAGACTCGGCCCTGTATCTCTGTGCCAGCAGCCAAGTGGAGCACG96400.12812275967918274No Hit
TTCTGCTTTCTTGACATCCGCTCACCAGGCCTGGGGGACGCAGCCATGTA95100.12639496312749252No Hit
GCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGCCTCGGAGGGGT92840.12339125527609257No Hit
AGCTGAATGTGAACGCCTTGTTGCTAGGGGACTCGGCCCTGTATCTCTGT89530.11899201944063516No Hit
AGTCAGCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTAAG85590.11375546681474326No Hit
GCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTGATCAGGGGGG84390.11216057769010614No Hit
GAGCTGAATGTGAACGCCTTGGAGCTGGACGACTCGGCCCTGTATCTCTG83070.11040619965300529No Hit
CTGAATGTGAACGCCTTGGAGCTGGGGGACTCGGCCCTTTATCTTTGCGC82130.10915686983870619No Hit
AGTCAGCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTGAT80630.1071632584329098No Hit
GCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGCCGGACAGGGGT80520.1070170602631514No Hit
AGTTGGCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTTCC80470.10695060654962484No Hit
TCTACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCCGCCGTGTATCT79940.10624619718624345No Hit
AGTTGGCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTAAG79820.10608670827377974No Hit
GCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTTACGGGCA78990.10498357662923904No Hit
AGTTGGCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTGAT76600.1018070891226701No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)