FastQCFastQC Report
Wed 12 Jul 2023
EGAF00008107302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008107302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14649093
Sequences flagged as poor quality0
Sequence length35
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACACACTGGTAGCCTGGCATTTAGTGTTTTTTT364390.24874577559170388No Hit
AGTGAGTCGAATTAACAGTTGGTGTGTTTTTTTTT348990.23823317935110386No Hit
GCGACGATAGTTGTACACGGTGTCCAGTTTTTTTT341870.23337281018012518No Hit
GGACCAACAGGATAACCAGTAAGATCTCTTTTTTT328110.22397973717553707No Hit
ACACGGGAACTTAGGGAGTGACCTTTTGTTTTTTT324670.22163146892439006No Hit
CACTCTTCTGCTAGCCCTTATGTGGGTATTTTTTT314380.21460714325453462No Hit
AATTCCAAGCATGTACCGACCTTGTTTTTTTTTTT313090.21372654266035448No Hit
AGTGGCGTCTGAAGGTTTTGAGGTGCTTTTTTTTT310150.21171959246896718No Hit
TTGGATATCGTCTACGTTTTAGACAGGTTTTTTTT296540.20242891488230705No Hit
TCTTGCTCCCGATACTCAAATGGGCATGTTTTTTT293550.20038783288494377No Hit
TCGGGAGACAGCGTACTTTCGACCTCGGTTTTTTT287040.19594387174687197No Hit
CCCAGTTAAGGCGCCGTCCTGGATGATATTTTTTT286980.1959029135797008No Hit
CTCACATTTACTAAATGTAAGCGTTTTGTTTTTTT274160.18715151852746106No Hit
AGGATATAGGGATTTAAGGGTTGGTTCTTTTTTTT272320.1858954680675452No Hit
TACGTTTACCGGCAATTTAAGTTATCGGTTTTTTT270840.18488516661065638No Hit
CGAGAGGGTAGCCGCGTGATTTTAGTCATTTTTTT259700.17728060023920936No Hit
AGGCACGTGACTGTCCAGATCGTGGAATTTTTTTT258110.1761952088091734No Hit
GTACGAGATTGCGACAGAAATCTGTGGCTTTTTTT257690.17590850163897517No Hit
AAAGGGATGTAGCAAGACAACGGCGAAATTTTTTT256550.175130296462723No Hit
GTCCGGCTGAATTGCGCCTTGATTGGTCTTTTTTT244910.1671844120315162No Hit
CCTATGAAGTGGTGCCAGTCGGAATTTTTTTTTTT239360.1633957815681831No Hit
TAATACTAGAACAGACAATGGACTGATTTTTTTTT236690.16157314312906607No Hit
GTTACAATTGGTGACGGTTTTACTGATGTTTTTTT236470.1614229631827718No Hit
AGTCTTCTCCTCAAATATTGGTCTTGGGTTTTTTT235770.16094511789910815No Hit
GCGAAACGATCGGGAGTTCGCATCTTTGTTTTTTT232850.15895182043011127No Hit
CGGGAATATAGTATACTAAGGTTTAACCTTTTTTT230670.1574636736895588No Hit
GCACACACTGGTAGCCGTATTCGAAGATTTTTTTT228010.15564786161163696No Hit
GACTAGGCCGTTAGGTTGATGTTGAGGTTTTTTTT227000.1549583991309223No Hit
ACCATCGTATATGGTAGTGTGCGTGGTATTTTTTT225690.1540641458143518No Hit
ACACGTAGGCCACAAGAATCATGAATTGTTTTTTT223690.15269887357531284No Hit
GAGTATGCCCGCCTTGGTCTTTGCGCTTTTTTTTT222680.15200941109459815No Hit
AGAACCCAGCGTGACATATTCTTCAAGGTTTTTTT219060.14953826834193762No Hit
CCCGAGTTTCTCCGTATTGATTCTTTGTTTTTTTT217810.14868497319253826No Hit
TGCTCGGTGGGTCACCCTCAGTCGACTATTTTTTT216520.14780437259835816No Hit
ATCATAGCCCTATGTACCATGTAACTGTTTTTTTT216320.14766784537445424No Hit
GAGTCCGCTTACCGGAGGCACTGGGAGGTTTTTTT213020.14541514618003995No Hit
CATCGGACGGGTTAATAGGTGTTGAGGATTTTTTT211790.144575503753031No Hit
ATTGCGATCAGTAACTATTCGGTCTGAATTTTTTT209380.14293035070498905No Hit
TGAACAAGCAGGGACTTACACTGGAGCCTTTTTTT209260.1428484343706467No Hit
CGTCGCATGTGAGCCAATATTTCTAGCGTTTTTTT207750.14181765383017228No Hit
TTAGTAGGGCGGCGGGAGTATTCACAGGTTTTTTT205900.14055477700906124No Hit
GAAATGGCGGTGTTAGTTGTGGTCACTATTTTTTT205370.1401929798657159No Hit
TCGCATAAAGGGCGCAAGAATAGCTGGATTTTTTT204610.1396741764148811No Hit
AGTACTCTTATGCCCACCACTGTACTATTTTTTTT204480.13958543371934357No Hit
AGAGCCGCCGAGATTTGACTAAATGGGTTTTTTTT204260.1394352537730493No Hit
TAGGTGTTCCACAGATGGACTTGCGACGTTTTTTT201970.13787201705934968No Hit
ACTGTCTTCTTTAGAATCATCTTTGGTTTTTTTTT201450.13751704627719954No Hit
GCGCTCGATCACCTGTTTTAAGATGCATTTTTTTT200910.13714842277265904No Hit
ATACCTAACCAAGAAAAGTGTCATTGAGTTTTTTT200700.13700506918755995No Hit
TGCATGTGGTAATCTAGCACGGGCATAGTTTTTTT200060.13656818207106747No Hit
GCTTAATGTAACTAACAGATGCATGATGTTTTTTT198990.13583776142318163No Hit
TCCGATGGTGCGACATGGTCGGGTCTCATTTCTTT197760.13499811899617264No Hit
GTGATCCTTGTCATGAGTTAGATATGTATTTTTTT197750.13499129263497747No Hit
TTGGTCACACTCGTAAATTTTTAACTAGTTTTTTT197390.13474554363195046No Hit
AGATGATGGAGTCTGGGTGTGAGGGTGTTTTTTTT196940.1344383573781667No Hit
CTGTGCAGGGTAGGTCAAAAGACATTCTTTTTTTT196610.13421308745872526No Hit
ATGTGCATCCGACGCATGATGTGTTATTTTTTTTT194710.13291607883163825No Hit
TAGGTGTTCCACAGATTATACTGGTTATTTTTTTT193690.13221978998972836No Hit
CATGTCTCATTTATGGACAAAACAGTTTTTTTTTT193140.13184434012399265No Hit
GACTGCACCAGCCCAGTCGCACTCTTTTTTTTTTT192500.13140745300750017No Hit
CGGGTGTACCCATTTATGTGTTTGAGTATTTTTTT192310.13127775214479148No Hit
GCAACGGCTAGTTATGCCTTGAGCATTTTTTTTTT192000.13106613494774044No Hit
GGGCAGTCAACGCCAATAAGATGCAGCTTTTTTTT190650.13014457618638914No Hit
TAGACTACCTAGCGTTTGGAAGGTGGTATTTTTTT190620.13012409710280357No Hit
GTGCAGCGTAGAGTAGGCTTAACATTTGTTTTTTT190200.12983738993260538No Hit
CGGGTTTGTTAGGGCTTCGCAGGATGGCTTTTTTT190140.1297964317654342No Hit
TGTAGCCAATTCCGTTTATTAGGCGCTTTTTTTTT188830.1289021784488637No Hit
TTCAGCTGGCGTGCCCGTGACGTTGGGATTTTTTT188640.128772477586155No Hit
CTGTGGTCGGGAGATAAGCACTTGTTTTTTTTTTT188220.1284857704159568No Hit
CCCAAGTCATTACACTGTCAATATGAAGTTTTTTT188200.12847211769356642No Hit
TCCAGAGCACCGGTTCGGTACGGGGTTGTTTTTTT186610.12738672626353045No Hit
AGGTTACACCATGCCGGGTATTCCTATCTTTTTTT186220.12712049817691784No Hit
CGGTCAAGTGGGAACCTAGAGTTGCCTCTTTTTTT186050.12700445003659952No Hit
AAGGATCGATCGCTTGGTGATAATCAATTTTTTTT185890.12689522825747643No Hit
CCGGGTTCGAGGTTACCATGTAGACTCGTTTTTTT184970.1262672030275185No Hit
TCAGTAGGGACTATAATACATCGGTTAGTTTTTTT184780.1261375021648098No Hit
TCCCTGGCTCGCTGGAGGGGACAGAAATTTTTTTT184600.12601462766329627No Hit
GCCGCACTCCGTTTCACGGTTCAGAAACTTTTTTT183340.12515450615270174No Hit
TCCGATGGTGCGACATATTTTGGGCTGGTTTTTTT182680.12470396631381889No Hit
CCCAATTTCACAACTTGCGGCAGAGTATTTTTTTT182410.12451965456154862No Hit
TGGGCAATAGTTGGGTTCCTTGTTGATGTTTTTTT181220.12370731757932044No Hit
TCTTAGAGCTCCAATTCTCGGCCCAGTTTTTTTTT180690.12334552043597512No Hit
TCGAGGGCAACAGACGCCTGACAGGGCTTTTTTTT180270.12305881326577692No Hit
GCGAAGCCATACCCGTGACTATCTCACTTTTTTTT179970.12285402242992109No Hit
GGAGGGTCAAGTAAGAGTGACTCTTGGGTTTTTTT179920.1228198906239451No Hit
GAGTATGCGCGTGCATGTTGACGTGATATTTTTTT179830.12275845337318836No Hit
GAAACCGAATTACCTTACGGTGGGAGTTTTTTTTT178560.12189150550139861No Hit
CCACTCAGATCCGCAAGACTCTTCGAATTTTTTTT177730.12132491752219746No Hit
AACCCGAGCAGAATCGAACAAAGGCTAATTTTTTT177440.12112695304753679No Hit
GCTATCATACTCATGGAAATACGATCGTTTTTTTT176500.12048527509518848No Hit
TGCGTACGGCTAATTGAAGGGTTTATAATTTTTTT176250.1203146160653086No Hit
AGGTTACACCATGCCGAACAATACTGTATTTTTTT175500.119802638975669No Hit
CCGCACAAAGACCAACGAGATGCCATAATTTTTTT174870.11937257822037173No Hit
TTCAGCTGGCGTGCCCGTAAGAGCCGAGTTTTTTT174610.11919509282929666No Hit
TCTTCGCGGTGAGAGGCCGACGCGGTCTTTTTTTT174580.11917461374571109No Hit
TTACTGGGATATTTCACCATAGTTTCATTTTTTTT174220.11892886474268406No Hit
CTTCGTAGATAGGTGATCGCTAAAATTTTTTTTTT172170.11752946069766912No Hit
TACCGAATAATTGTAAACCTTTACCGAATTTTTTT171940.11737245439017965No Hit
GTTAACTATGTTGTCAAGGACTGCTTGTTTTTTTT171780.11726323261105652No Hit
GCTCCGCTCGCTTCAGTAGATGTAAACGTTTTTTT169500.11570682225855212No Hit
CACTGACGATTGTGGAGATAGTTAACAATTTTTTT169100.11543376781074433No Hit
CTCTGGACGCCTGGTGGCTCAGGCTCGGTTTTTTT169060.11540646236596354No Hit
CCTATAATGAGTGCCCCGAGGCTAGTTGTTTTTTT169000.11536550419879238No Hit
CCGCACAAAGACCAACCGTTTATCTGGTTTTTTTT168930.115317719670426No Hit
TGTACGAACAAATCCGTTTTTTTTTTGCTTTTTTT168520.11503783886142303No Hit
GCATGAGGGACGCGGCCACCATGACATTTTTTTTT167970.1146623889956873No Hit
TAAAGACAACCCTTTATCTCTATTGATGTTTTTTT167730.11449855632700263No Hit
GCGTCTCTGCATTGGGGTGGAATGAAGATTTTTTT166570.11370669842836004No Hit
GTAGCTTCCTCTTGTTCCAGCACAAGTTTTTTTTT166540.11368621934477446No Hit
AGCGTGGTATTCTACTTTTTGGGTACCTTTTTTTT165950.11328346403425796No Hit
ACACTGGGACAGTCGTCTGATACTTACTTTTTTTT165850.113215200422306No Hit
CGAGAGGGTAGCCGCGGCTTCGGAGTTTTTTTTTT165600.11304454139242613No Hit
CTCGCACCTATATAGTCGTCGGTGTCCATTTTTTT165230.11279196602820393No Hit
CGACACGCTCCGACAGCTTATCAGATGCTTTTTTT164810.11250525885800573No Hit
TATCCAATTGGTTATCAACATGTGCACCTTTTTTT164450.11225950985497873No Hit
CTTCGATTGCGCAAGCTTGCTAGATGTTTTTTTTT163470.11159052645784964No Hit
GGTCGGCCAGGAGCTTATCACAGATCGATTTTTTT162670.11104441756223406No Hit
GCAGACCCAGCACGTACAGTACTTTTATTTTTTTT162220.11073723130845028No Hit
TTGAGCAGCCCACGGTGTGTGATACGTATTTTTTT161770.11043004505466653No Hit
TCTTGGTAACACCAAAATCCATGTGTTCTTTTTTT161590.110307170553153No Hit
GCGCTCGATCACCTGTGTACTGCGAAAATTTTTTT161260.11008190063371158No Hit
GTACGCTTCATTGCACCAATTTCAGCACTTTTTTT161050.10993854704861249No Hit
CCAACGATGCACTGATCGCTATTGGCCGTTTTTTT160990.10989758888144133No Hit
ATCACTTCATCCTCGCTTTGTACGCGAATTTTTTT159440.10883950289618613No Hit
GATACGATGGGAGTCATTTACCATGCCCTTTTTTT159390.10880537109021016No Hit
CTCGGTACCACTGCTCCGTATTTCTGTCTTTTTTT159060.10858010117076873No Hit
CATAGAGGAGATACTAACCGAGACATCTTTTTTTT158430.10815004041547145No Hit
TCACCAAATCTTAAACTGAGTAGTCAATTTTTTTT157990.1078496805228829No Hit
ACCACCAATGTAACAATTAGAACGGGTCTTTTTTT157940.1078155487169069No Hit
TGTCCTAAGTCACCGCCGGTCTTTTAGGTTTTTTT157830.10774045874375976No Hit
GGCAGCAAACCTATGCGGTAAATACGAGTTTTTTT157830.10774045874375976No Hit
CACAGTCCCGCTTCGCCTGGTTCTCGGGTTTTTTT157580.10756979971387991No Hit
GACTCACCCACGTGAGTACCGTTGTGGTTTTTTTT157550.1075493206302943No Hit
TCGGAGTACATGAGTACACAATATGTCCTTTTTTT156970.10715339168097301No Hit
CGGAACGTAAACATAGCTTACCTGTATATTTTTTT156890.10709878079141145No Hit
CTGGTAAAGTGTGGGCCGTGAAGTCTTTTTTTTTT156820.1070509962630451No Hit
ACATGGCGCCAAAGTATAACCAACAATATTTTTTT156760.10701003809587392No Hit
GGTCGCCAGGAGCTTCATTAATTTCTGTTTTTTTT156600.10690081631675082No Hit
ACGTTAATGTCGAAGACTTTGCAAAACGTTTTTTT155980.10647758192264872No Hit
ACTCGCCGTTCGATAAGTGCCTTTTAGATTTTTTT155860.10639566558830638No Hit
ATCAATCTGGGCTGCAATTGCATTGCGCTTTTTTT154620.10554919680010225No Hit
GTGCAGCGTAGAGTAGGGATTAGGTCCGTTTTTTT154550.10550141227173586No Hit
TTCGGTGGAGACGCCCCAGTTTAAATTTTTTTTTT154370.10537853777022237No Hit
TCTACCGTCCACAAGCTTATGTCCTTACTTTTTTT154240.10528979507468483No Hit
AGCAAAGGCCGCTAGTGGAAGCCGTATTTTTTTTT154170.10524201054631847No Hit
GTATAGGACTCAGTAGTGTTTGTAAATTTTTTTTT154010.10513278876719534No Hit
ACACGGGAACTTAGGGGTGTGTGTTTAATTTTTTT153380.10470272801189809No Hit
TGATTTATTAGCTGTGTGACAACATGTTTTTTTTT153330.1046685962059221No Hit
TACGTGGGCCCAGGGCAGCGGTCGGAGATTTTTTT153220.10459350623277495No Hit
TGCCGTGGATCGTCCTCCGTCCATTTCGTTTTTTT152160.1038699119460843No Hit
CGCATGGTGCGATGCTCCACGGAGTATATTTTTTT151500.10341937210720145No Hit
CAAACGAGTATCGCAGCACTTACTATGTTTTTTTT151180.10320092854895521No Hit
GGCTTTGCTCCAACGCCTAAGTATGTCGTTTTTTT151100.10314631765939365No Hit
CCTGTTTGAAGACACGTATGCAGCGCTCTTTTTTT150940.10303709588027055No Hit
TCCGCGGCAGCATCTGGTCTTCCAAAATATTTTTT150930.10303026951907533No Hit
CTGCTGTCTAACGAGCCCTCCTGCCGATTTTTTTT150540.10276404143246273No Hit
GCTAGGCACCACGGAGCCTTCAATGACTTTTTTTT149970.10237493884433664No Hit
ACTATCCAGGGCATGGGAGTTCTATCGGTTTTTTT149950.10236128612194627No Hit
AGGCATTGTCGTAGGGCGGTACGACCTCTTTTTTT148220.10118032563517755No Hit
TAGGTGTTCCACAGATAGATTTTGCATTTTTTTTT147880.10094822935454092No Hit
GACTCACCCACGTGAGTTTTCCAGGTGGTTTTTTT147420.10063421673956197No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)