FastQCFastQC Report
Wed 12 Jul 2023
EGAF00008107303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008107303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14649093
Sequences flagged as poor quality0
Sequence length259
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGAATGTGAGCACCTTGGAGCTGGGGGACTCGGCCCTTTATCTTTGC2553551.743145462998972No Hit
CTGAATGTGAACGCCTTGGAGCTGGACGACTCGGCCCTGTATCTCTGTGC2085791.4238355917325394No Hit
GAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTGTATCTCTG1795621.2257550689315715No Hit
GAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTCTATCTCTG1542961.053280226973779No Hit
TCTACTCTGAAGATCCAGCGCACAGAGCGGGGGGACTCAGCCGTGTATCT1491591.0182132095140635No Hit
AGTCAGCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTTAC1102760.7527838071613034No Hit
AGTTGGCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTTAC1097250.7490224821427511No Hit
CAAGATCCAGCCTGCAAAGCTTGAGGACTCGGCCGTGTATCTCTGTGCCA1015680.693339853873547No Hit
AGGATCCAGCAGGTAGTGCGAGGAGATTCGGCAGCTTATTTCTGTGCCAG1012120.6909096692880576No Hit
GGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAGAGCAGGGG950750.649016290633147No Hit
CTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGAC912250.6227348000316469No Hit
ATAGCTCTGAGCTGAATGTGAACGCCTTGGAGCTGGACGACTCGGCCCTG851990.581599147469403No Hit
GATTTCCTCCTCACTCTGGAGTCCGCTACCAGCTCCCAGACATCTGTGTA741710.5063180362087946No Hit
CATTCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTG714520.48775716011905995No Hit
CATCATTCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCT706630.4823711611360512No Hit
AGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTGTATCTCTGT684320.46714154930957164No Hit
AGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTCTATCTCTGT604390.4125784442763794No Hit
GAACTGAACATGAGCTCCTTGGAGCTGGGGGACTCAGCCCTGTACTTCTG559590.38199634612190664No Hit
TATTCCTTCACCTACACACCCTGCAGCCAGAAGACTCGGCCCTGTATCTC558880.3815116744770478No Hit
GCCAGAAGACTCGGCCCTGTATCTCTGCGCCAGCAGCAACGACAGGGGCG552170.3769311861150721No Hit
AACTATAGCTCTGAGCTGAATGTGAACGCCTTGGAGCTGGACGACTCGGC509650.34790549831310374No Hit
AACTATAGCTCTGAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGC504470.3443694432139928No Hit
GGGACTCAGCTTTGTATTTCTGTGCCAGCAGCCTCGCCTCGGAGTCAGGC415570.28368309218871096No Hit
ATAGCTCTGAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTG405180.2765905029069035No Hit
GGGACTCAGCTTTGTATTTCTGTGCCAGCAGCGTAGGGGACACTGAAGCT380010.2594085517785982No Hit
TTCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCG377440.2576541769514331No Hit
ATAGCTCTGAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTC346390.2364583254403532No Hit
TCTACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCCGCCGTGTATCT328310.22411626439944096No Hit
GCCAGAAGACTCGGCCCTGTATCTCTGTGCCAGCAGCCAAGAGGGGGGAG259410.17708263576454872No Hit
ACCAGACCTCTCTGTACTTCTGTGCCAGCTGGAAGTGGACTAGTGAAGCT247650.1690548349989996No Hit
GCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTTACGGGCA247600.16902070319302362No Hit
GCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGCCTCGGAGG242430.1654914744551079No Hit
AGCTGAATGTGAACGCCTTGTTGCTAGGGGACTCGGCCCTCTATCTCTGT241770.16504093461622504No Hit
AGTCAGCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTGAA221700.15134042769746905No Hit
GCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGCTCGACAGG218640.14925156117173943No Hit
TTCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATTCAGCG214630.14651419033246632No Hit
CTGAATGTGAACGCCTTGGAGCTGGAGGACTCGGCCCTGTATCTCTGTGC212410.14499873814713307No Hit
AGTTGGCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTGAA211080.14409083210817217No Hit
CTGAATGTGAACGCCTTGGAGCTGGGGGACTCGGCCCTTTATCTTTGCGC202760.13841129959377008No Hit
AGCTGAATGTGAACGCCTTGTTGCTAGGGGACTCGGCCCTGTATCTCTGT198320.13538039522310358No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG197050.13451344735131385No Hit
GCCAGAAGACTCGGCCCTGTATCTCTGTGCCAGCAGCTTGAGACTAGCGG188760.12885439392049733No Hit
GCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGCCTCGGAGGGGT183800.1254685187676807No Hit
GCCAGAAGACTCGGCCCTGTATCTCTGTGCCAGCAGCCAAGATGCTATTC174260.11895617018746485No Hit
GCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGCTCGACAGGGGC165760.11315376317154927No Hit
GAGCTGAATGTGAACGCCTTGGAGCTGGACGACTCGGCCCTGTATCTCTG162260.11076453675323107No Hit
GCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTGATCAGGG157490.10750836246312315No Hit
CATTCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCCGCCGTG155800.1063547074211352No Hit
GCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTTACTCGGC153650.10488703976416834No Hit
AGTTGGCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTTAT152000.1037606901669612No Hit
AGTCAGCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTTCC151930.10371290563859484No Hit
AGTTGGCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGCTCG148390.10129637377549587No Hit
GCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTTACGGGCAGAA148300.1012349365247391No Hit
TTCTGCTTTCTTGACATCCGCTCACCAGGCCTGGGGGACGCAGCCATGTA148270.10121445744115352No Hit
GCCAGAAGACTCGGCCCTGTATCTCTGTGCCAGCAGCCAGCAGGGTGCAC147340.1005796058500004No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)