FastQCFastQC Report
Wed 12 Jul 2023
EGAF00008107307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008107307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13293817
Sequences flagged as poor quality0
Sequence length259
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGAATGTGAGCACCTTGGAGCTGGGGGACTCGGCCCTTTATCTTTGC3532702.6574008052013953No Hit
GAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTGTATCTCTG1658661.2476928183982072No Hit
TCTACTCTGAAGATCCAGCGCACAGAGCGGGGGGACTCAGCCGTGTATCT1278080.9614093529345259No Hit
CTGAATGTGAACGCCTTGGAGCTGGACGACTCGGCCCTGTATCTCTGTGC1126750.847574477668829No Hit
GAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTCTATCTCTG957030.7199061037172394No Hit
CATTCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTG930080.699633521358087No Hit
GATTTCCTCCTCACTCTGGAGTCCGCTACCAGCTCCCAGACATCTGTGTA889870.669386377140591No Hit
AACTATAGCTCTGAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGC813950.6122771210104667No Hit
ATAGCTCTGAGCTGAATGTGAACGCCTTGGAGCTGGACGACTCGGCCCTG805640.6060260946874776No Hit
CATCATTCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCT802900.6039649861285137No Hit
ATAGCTCTGAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTG758140.5702951981360959No Hit
TATTCCTTCACCTACACACCCTGCAGCCAGAAGACTCGGCCCTGTATCTC739080.5559577057514783No Hit
AGGATCCAGCAGGTAGTGCGAGGAGATTCGGCAGCTTATTTCTGTGCCAG716240.5387767862307719No Hit
GGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAGAGCAGGGG680700.5120425533163274No Hit
CTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGAC635120.47775593721502263No Hit
CAAGATCCAGCCTGCAAAGCTTGAGGACTCGGCCGTGTATCTCTGTGCCA609340.45836346325513583No Hit
GGGACTCAGCTTTGTATTTCTGTGCCAGCAGCCTCGCCTCGGAGTCAGGC608870.4580099154366274No Hit
AGTTGGCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTTAC608250.4575435332079568No Hit
AGTCAGCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTTAC591360.44483837862368647No Hit
GAACTGAACATGAGCTCCTTGGAGCTGGGGGACTCAGCCCTGTACTTCTG567010.4265215927073466No Hit
AGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTGTATCTCTGT514910.38733044091098895No Hit
AACTATAGCTCTGAGCTGAATGTGAACGCCTTGGAGCTGGACGACTCGGC501090.37693463058803955No Hit
ATAGCTCTGAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTC428660.3224506550676905No Hit
TTCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCG395680.29764212942001533No Hit
GCCAGAAGACTCGGCCCTGTATCTCTGCGCCAGCAGCAACGACAGGGGCG382140.28745694332936883No Hit
ACCAGACCTCTCTGTACTTCTGTGCCAGCTGGAAGTGGACTAGTGAAGCT364460.274157527518244No Hit
GCCAGAAGACTCGGCCCTGTATCTCTGCGCCAGCAGCCAAGTTGAAGCCG358560.26971937405186186No Hit
CTGAATGTGAACGCCTTGGAGCTGGGGGACTCGGCCCTTTATCTTTGCGC317330.2387049558452625No Hit
AGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTCTATCTCTGT305500.22980608203046574No Hit
ATCTTTCTCCACCTGAAGATCCAGCGCACAGAGCGGGGGGACTCAGCCGT253850.19095343346459487No Hit
GCCAGAAGACTCGGCCCTGTATCTCTGTGCCAGCAGCCAAGATAATAAGA248050.18659050293832086No Hit
GCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTCAACAGGG241150.18140012007085698No Hit
TCAGGGGACACAGCACTGTATTTCTGTGCCAGCAGCAAGAGCGGGACAGA237360.1785491706407573No Hit
ACCCGACAGCTTTCTATCTCTGTGCCAGTAGTAGGGGGTGGCAGCCCCAG237320.178519081464714No Hit
GGGACTCAGCTTTGTATTTCTGTGCCAGCAGCGTAGGGGACACTGAAGCT235330.17702214495656138No Hit
AGCTGAATGTGAACGCCTTGTTGCTAGGGGACTCGGCCCTGTATCTCTGT226880.17066580651742086No Hit
AGTTGGCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTGAA191100.14375103854671686No Hit
GCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTCAACAGGGTCT189910.14285588555942963No Hit
AGTCAGCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTGAA186940.1406217642382169No Hit
TAGACTCCACTCTCAAGATCCAGCCTGCAAAGCTTGAGGACTCGGCCGTG179770.135228279432461No Hit
ACCCGACAGCTTTCTATCTCTGTGCCAGTAGAGTACTAGCGGGGGACAAT178610.13435569332720618No Hit
GCTGCTCCCTCCCAAACATCTGTGTACTTCTGTGCCAGCAGTTACCTTCC174690.13140695407496583No Hit
ATCTTTCTCCACCTGAAGATCCAGCGCACACAGCAGGAGGACTCCGCCGT171850.12927062257589375No Hit
GCCAGAAGACTCGGCCCTGTATCTCTGTGCCAGCAGCTTGAGACTAGCGG168630.1268484439044106No Hit
TTCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATTCAGCG167030.12564487686267986No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG163300.12283906119664502No Hit
CACAAAGCTGGAGGACTCAGCCATGTACTTCTGTGCCAGCAGTATGGGAG162580.1222974560278662No Hit
GCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTTACTCGAG161580.12154522662678446No Hit
GCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGCCGGACAGG154990.11658803487365592No Hit
GTGCCCATCCTGAAGACAGCAGCTTCTACATCTGCAGTGCTGAGCGGGAG150900.11351141662323169No Hit
CTGAATGTGAACGCCTTGGAGCTGGGGGACTCGGCCCTGTATCTCTGTGC149320.11232289416952257No Hit
ACCAGACCTCTCTGTACTTCTGTGCCAGCAAAAATAGCAATCAGCCCCAG149280.11229280499347931No Hit
TCTACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCCGCCGTGTATCT148070.11138260741817042No Hit
GCTCCCTCCCAAACATCTGTGTACTTCTGTGCCAGCAGTTACCTTCCCGG146570.11025426331654783No Hit
GCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTTACGGGCA146380.11011133973034232No Hit
GGGACTCAGCTTTGTATTTCTGTGCCAGCAGCACCCGGGCAGGGGTTGGA143250.10775686170495652No Hit
GTGCCCATCCTGAAGACAGCAGCTTCTACATCTGCAGTGCTGCCGCCGGC142200.10696702083382072No Hit
GCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTATCCGGGA140210.10547008432566808No Hit
AGCTGAATGTGAACGCCTTGTTGCTAGGGGACTCGGCCCTCTATCTCTGT135960.10227310937107079No Hit
AGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAACCTGCAAAGCTTGA134180.10093414103714532No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)