FastQCFastQC Report
Wed 12 Jul 2023
EGAF00008107343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008107343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390764
Sequences flagged as poor quality0
Sequence length122-3433
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGTGGGTTTACCCGCCAAGCGG17690.4527029101964357Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGACAAGACCACGCCTCCCGTG14940.3823279524214104Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGACACCCTGCCCCCATCCCGG13260.339335250944304Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTTTCTGAGAGTCATGGA11400.2917361885946505Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGCAAGCTCACCGTGGAC10480.26819256635718747Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCTGTGGGCTCAGAAGCA9240.23645985812408513Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTACGTGCCAAGCATCCTCG9030.23108577043944684Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGATGCTTTCTGAGAGTCATG8960.2292944078779007Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAAGCTCACCGTGGACAA8480.21701077888444173Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGACCAGGACTGGCTGAATGGC8280.2118926001371672Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCTTCAGCTGTGGGTAGA7930.20293578732943668Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGACAACAGCACGTACCGTGTG7690.1967939728327072Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGTGACTTCTACCCGGGAG7120.1822071634029747Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGCCGTGGAGTGGGAGAG6580.16838808078533335Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATGCGTGGTGGTGGACGTGA6540.16736444503587844Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGACATTCCGGCCCGAGGTCCA6160.15763990541605677Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGACCACGCCTCCCGTGCT5750.14714763898414387Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAGCTCTGGGGAGTCTGC5450.13947037086323202Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAATGCCAAGACAAAGCCG5370.13742309936432218Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATCCCAGCGACATCGCCGTG5230.13384037424123Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGGACACCCTCATGATCTC5160.13204901167968391Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGACACGCAGAAGAGCCTCTCC5110.13076946699286526Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTGGGGTCTCAGGAGGCA4790.12258038099722596Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTCTGGGCCTAAGGAAGC4710.12053310949831611Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGTGTACACCCTGCCCCCA4640.11874174693677002Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTGTGGGCTCAGAAGCAG4610.11797402012467884Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATCTCCAAAGCCAAAGGGCA4490.1149031128763141Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGGAACTGCTCAGTTAGG4400.11259993244004053Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGCTGCAGGATCCCAGGG4360.11157629669058561Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAGCAACAACAAGTACGC4270.10927311625431206Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGAACTGCTCAGTTAGGA4250.10876129837958462Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAACCAGGTCAGCCTGAC4230.10824948050485715Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTCTGAGAGTCATGGAC4190.10722584475540224Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAGTGACTTCTACCCGGG4140.1059463000685836Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)