FastQCFastQC Report
Wed 12 Jul 2023
EGAF00008107345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008107345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385114
Sequences flagged as poor quality0
Sequence length65-4780
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGTCTCAGGAGGCAGCG13760.3572968004279252Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTGGGGTCTCAGGAGGCA11610.30146917536106194Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTGGGCCTCAGGAAGCAGC8410.21837689619177697Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAGCTCTGCTTCAGCTGTG8100.2103273316472525Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTCGGGTAACTCCCAGGAGAG7740.20097945024070796Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTCTGGGCCTAAGGAAGC7240.1879962816205072Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGACAGAAGACCATCGACCGCT6340.1646265781041458Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCAGGTGGCAGCAGGG6270.16280893449731768Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCTCTGCTTCAGCTGTG6020.1563173501872173Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTGGGGTCTCAGGAGGCAG5940.15424004320798518Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGGAACTGCTCAGTTAGG5830.151383746111541Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTGGGTTTACCCGCCAAGCGG5540.14385350831182456Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGTCACAAGAGGCAGCG5510.1430745181946125Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGTCTCAGGAGGCAGCA5150.13372663678806795Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGAACTGCTCAGTTAGGA5120.1329476466708559Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGACATTCCGGCCCGAGGTCCA4910.1274947158503716Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGGCCTGAGCTCGCCCGTC4910.1274947158503716Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGACAGCAAGCTCACCGTGGAC4880.12671572573315953Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGATGCATGAGGCTCTGCA4780.12411909200911939Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGCGGTCCTCCAGCTCAT4690.12178212165748323Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCTTCAGCTGTGGGTAGA4680.12152245828507921Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGATCAGGACTCCTCAGTTC4550.11814683444382702Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTACGTGCCAAGCATCCTCG4370.11347289374055475Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGCTACAACAGGCAGGCA4330.11243424025093869Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAGAGCCTCTCCCTGTCT3980.10334602221679814Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCTCAGGTGTCACCTTCA3930.10204770535477807Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAGACCATCGACCGCTTG3930.10204770535477807Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)