FastQCFastQC Report
Wed 12 Jul 2023
EGAF00008107350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008107350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354737
Sequences flagged as poor quality0
Sequence length74-5008
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGTCGGGTAACTCCCAGGAGAG6560.18492573371258142Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACGCAGCCACCTCACAGGT5800.16350141090441653Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCTCAGGTGTCACCTTCA5470.15419874442192386Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGTCTCAGGAGGCAGCG5470.15419874442192386Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGACATTCCGGCCCGAGGTCCA4710.1327744216137589Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACAGTGGAAGGTGGATAAC4360.12290795716263035Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC4020.11332339169581973Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACAAATGCGTGGTCCAGCACACCGCCA3720.10486642216628093Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTAACGCCCTCCAATCGGGTA3700.10430262419764502Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAGCTCTGCTTCAGCTGTG3580.1009198363858295Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)