FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008109443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008109443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43280481
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCG4367351.0090807447357157No Hit
CGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATA1999820.4620604840320513No Hit
CCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCT1968020.4547130610678749No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGT1856960.42905253294204376No Hit
CCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGAT1689430.3903445527788843No Hit
GTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCG1415120.32696494292658157No Hit
GTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAA1400820.3236609131030683No Hit
GTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCC1225510.2831553558750884No Hit
GGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACG1217160.2812260797194005No Hit
CTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC1200900.277469189864133No Hit
GGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCA1154940.2668500842215686No Hit
GTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACAA1020390.23576216724578453No Hit
CCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCG890190.205679322279251No Hit
GTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGAT844820.19519653674828613No Hit
CTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCC812330.18768968856884932No Hit
CTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCATGCC806510.18634497153578308No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCG802630.1854484935137389No Hit
GTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTAT796070.18393279871358179No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA745250.1721907850330961No Hit
GAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG715670.1653562953701924No Hit
CGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCT715480.16531239567323663No Hit
CCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTA669890.1547787789142177No Hit
GTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGC649260.15001219602896743No Hit
CTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGC647110.14951543630025738No Hit
GTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCCCGTTC645180.1490695077996014No Hit
CTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCA616480.1424383430489139No Hit
CTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGG596030.13771334935025328No Hit
CTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAGA588790.1360405398452018No Hit
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTA558800.1291113192572883No Hit
GGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTA544780.1258719837240256No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTA541960.12522041980078732No Hit
ACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTC521730.12054625733018078No Hit
AGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCT521040.12038683211492035No Hit
CACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCA503560.11634805999498941No Hit
GAAGGACTTGGGCCCCCCACGAGCGGCGCCGGGGAGCGGGTCTTCCGTAC487750.11269514310619606No Hit
GGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTG485860.11225845664700446No Hit
GATCCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCC482960.11158840864083742No Hit
CTTGGGCCCCCCACGAGCGGCGCCGGGGAGCGGGTCTTCCGTACGCCACA478440.1105440579553633No Hit
GCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAA473260.10934721358572702No Hit
CATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCAC470660.10874648089054278No Hit
GGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTT469700.10852467189539784No Hit
CGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAA462270.10680796269339059No Hit
GTTACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGT457100.10561342883412041No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATT452330.10451131538949394No Hit
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC451630.1043495796638674No Hit
GCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAG449550.10386899350772003No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA448400.10360328481561931No Hit
GTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGG447820.1034692752143859No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)