FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008109447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008109447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31535158
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCG3071160.9738844498575209No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA1536820.48733543684797775No Hit
GGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCC1152310.3654048601881113No Hit
GAACTCTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACC1086850.3446470761300768No Hit
CTCTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCA1072390.340061717781785No Hit
GGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCAT1023910.3246884001659354No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC935930.2967893802846968No Hit
CCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGGATCTGAACCCGAC911520.2890488133910729No Hit
GAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG910410.28869682530209617No Hit
GTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAG840060.2663883910142451No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC815970.25874929816429015No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN811920.2574650172991047No Hit
GCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTC797880.2530128436331284No Hit
GCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCACGGGCCCCT764210.24233587160083359No Hit
GTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCC760560.24117843329023433No Hit
AGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACG754470.2392472553966592No Hit
TCTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCAC750260.23791223750963925No Hit
GTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGG739770.23458579151561568No Hit
GTTGAGATCGTTTCGGCCCCAAGACCTCTAATCATTCGCTTTACCGGATA708770.22475549353518381No Hit
GTTCACCTTGGAGACCTGCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCA679070.21533743385715715No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTA674000.21372970447777687No Hit
CTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCC636800.2019333469012586No Hit
GTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCG628240.19921891623311352No Hit
CTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGGATCTGAACCCGACT620830.19686915790940387No Hit
GGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTG620360.19672011790776503No Hit
GGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCA619780.196536196203615No Hit
GGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCAGTTA605470.19199840381329308No Hit
GCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGCCCGGCGCGCC587930.18643635779468742No Hit
CGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACG578880.18356654499717426No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA574170.18207297391692157No Hit
GCCGGAACCGCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAAC568550.1802908360249852No Hit
CCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGC545820.1730830078606234No Hit
GCTATGAACGCTTGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAA523040.1658593243769383No Hit
GCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCACGG518390.1643847796798735No Hit
CCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGAC501310.158968602599042No Hit
GGGTAGCCGACGTCGCCGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCT486690.15433250722891576No Hit
GGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACG479670.15210642039592762No Hit
CGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCACGGG477190.1513199965574931No Hit
CCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTCCG474140.15035282207877315No Hit
GGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGA466690.14799037949960483No Hit
GTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGG465700.14767644417700396No Hit
GTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTTATCTAGAGTCACC464020.14714370544774186No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT463050.14683611225287027No Hit
GAGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACG455110.14431828754433385No Hit
GCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGCCGCGCCCCGTTTCCC449460.14252663646080352No Hit
GTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTT445730.14134382963928704No Hit
GAGAACTCTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCC441090.13987245600608691No Hit
GGTTGAGATCGTTTCGGCCCCAAGACCTCTAATCATTCGCTTTACCGGAT432220.13705972235813754No Hit
CGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCAC431040.1366855368221082No Hit
CGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAA417920.13252510103168028No Hit
GTTTCCTCTGGCTTCGCCCTGCCCAGGCATAGTTCACCATCTTTCGGGTC412860.1309205427161646No Hit
TCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGG406330.1288498380125446No Hit
GAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGA403410.1279238873640652No Hit
GTTTCGGCCCCAAGACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCGGGTCTGCG400550.12701696309877375No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA396390.1256978005310771No Hit
GATAAAACTGCGTGGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACC394820.12519994350432617No Hit
GTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTTATCTAGAGTC393460.12476867881873305No Hit
CTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCG390650.12387760987276487No Hit
AGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGG383040.12146443027176207No Hit
GGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAG381110.12085241494588359No Hit
GTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGGC376570.11941275195133001No Hit
GAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCC369140.11705665149989102No Hit
GTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAG356810.11314672975477084No Hit
GTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGG351160.11135507867124053No Hit
AGAGAACTCTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTT346860.10999152120943868No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC346790.1099693237623861No Hit
ATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCC345610.10959513822635675No Hit
GTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGACGGGCCGGTGG341800.10838696289392302No Hit
GGCTGTTCACCTTGGAGACCTGCTGCGGATATGGGTACGGCCCGGCGCGA337500.10702340543212117No Hit
CGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGC334340.10602134925089007No Hit
TCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTG332990.1055932556291616No Hit
TGAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCG332520.10544421562752278No Hit
TGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACA329710.1045531466815546No Hit
GGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGC329470.10447704114880288No Hit
TCTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC327140.10373818326833814No Hit
CTTGGAGACCTGCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCC325940.10335765560457949No Hit
CTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACA322530.10227632282673199No Hit
GGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCG322440.1022477832519501No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)