FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008109451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008109451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32817991
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCG3190780.9722654869397704No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA1638240.49918960609136614No Hit
GGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCC1227440.3740143630364211No Hit
GAACTCTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACC1142430.3481108883234199No Hit
CTCTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCA1121440.34171500625982865No Hit
GGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCAT1070300.3261320901696877No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC989000.30135909294386726No Hit
GAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG976290.29748621723980606No Hit
CCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGGATCTGAACCCGAC957760.29183992402216213No Hit
GTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAG883510.2692151387328981No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC855850.26078683487968535No Hit
GCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTC846260.2578646572241427No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN844600.2573588371085847No Hit
GCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCACGGGCCCCT811100.24715102152352958No Hit
GTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCC802470.24452136634445418No Hit
AGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACG788210.24017618872526353No Hit
TCTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCAC782520.2384423836303691No Hit
GTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGG779740.237595287292266No Hit
GTTGAGATCGTTTCGGCCCCAAGACCTCTAATCATTCGCTTTACCGGATA750690.22874343527000177No Hit
GTTCACCTTGGAGACCTGCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCA716040.21818520213501186No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTA704100.21454695383395042No Hit
CTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCC672890.20503692623963485No Hit
GGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTG664500.20248040167967626No Hit
GTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCG658400.20062166511045726No Hit
GGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCA650810.19830890928088804No Hit
GGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCAGTTA648780.197690346127525No Hit
CTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGGATCTGAACCCGACT645440.19667261167814937No Hit
GCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGCCCGGCGCGCC625670.19064847692840187No Hit
GCCGGAACCGCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAAC604630.18423735931916127No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA603890.1840118732435511No Hit
CGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACG600890.1830977405045909No Hit
CCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGC565900.17243590565918554No Hit
GCTATGAACGCTTGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAA552420.16832840255212456No Hit
GCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCACGG549730.16750873019619025No Hit
GGGTAGCCGACGTCGCCGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCT522420.1591870751625229No Hit
CCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGAC521400.15887627003127644No Hit
GGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACG501490.15280947575371082No Hit
CGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCACGGG499590.15223052501903606No Hit
CCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTCCG496380.1512524029883487No Hit
GTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTTATCTAGAGTCACC494560.15069782912671284No Hit
GGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGA491880.14988120387990844No Hit
GTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGG488560.14886956364879253No Hit
GCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGCCGCGCCCCGTTTCCC487590.1485739940631954No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT484650.14767814397901444No Hit
GAGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACG477270.14542937744117243No Hit
GTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTT476850.145301398857718No Hit
GAGAACTCTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCC466850.14225428972785079No Hit
GGTTGAGATCGTTTCGGCCCCAAGACCTCTAATCATTCGCTTTACCGGAT461040.14048391932339796No Hit
CGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCAC456680.13915537974277584No Hit
CGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAA447210.13626976739679159No Hit
GTTTCCTCTGGCTTCGCCCTGCCCAGGCATAGTTCACCATCTTTCGGGTC432760.13186669470413348No Hit
GAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGA431370.13144314653508193No Hit
TCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGG430940.13131212084249766No Hit
GTTTCGGCCCCAAGACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCGGGTCTGCG426710.1300231936805638No Hit
GTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTTATCTAGAGTC421720.12850268622476008No Hit
GATAAAACTGCGTGGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACC416410.1268846712768006No Hit
CTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCG415600.12663785543728134No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA408780.1245597270107119No Hit
AGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGG407660.12421845078816676No Hit
GGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAG403310.12289295831667453No Hit
GTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGGC399010.12158270139083163No Hit
GAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCC396610.1208513951996635No Hit
GTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGG379110.11551895422239587No Hit
GTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAG378220.1152477615098377No Hit
ATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCC374050.11397711700268306No Hit
GTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGACGGGCCGGTGG364720.11113416418451695No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC363300.11070147468807581No Hit
AGAGAACTCTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTT358020.10909260106750593No Hit
TGAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCG356040.10848927345979223No Hit
GGCTGTTCACCTTGGAGACCTGCTGCGGATATGGGTACGGCCCGGCGCGA352330.1073587959726115No Hit
TGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACA350250.1067249972735991No Hit
TCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTG349590.10652388807102786No Hit
CTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACA346980.10572859258813253No Hit
GGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGC346870.105695074387704No Hit
CGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGC346540.10559451978641837No Hit
GGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCG345440.10525933778213296No Hit
TCTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC344710.10503689881565267No Hit
CTTGGAGACCTGCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCC341840.10416237849538079No Hit
GGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCACGTC331140.10090197172642286No Hit
GGATAAAACTGCGTGGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTA328630.10013714733482619No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA328470.10008839358874831No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)