FastQCFastQC Report
Mon 4 Sep 2023
EGAF00008109567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008109567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35223156
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT6215391.7645749858417004No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG2885430.8191855380591109No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA2285210.6487805919492279No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1336640.3794776368136915No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA1326290.37653922890952757No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC1076400.3055944220330512No Hit
GTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTA958650.2721647089204613No Hit
ATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGG784580.2227455143428942No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGA774480.2198780824750627No Hit
GGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTA747660.2122637732973161No Hit
CCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAA733680.2082947933456048No Hit
CAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTT723700.2054614299752129No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG716610.20344854958482425No Hit
GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT703850.19982593269041535No Hit
CTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGC700020.19873857981380205No Hit
CTTGAAGCCTAGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAGATCT688040.1953374081527504No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA649910.18451214309132322No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC645050.18313236894502016No Hit
TGTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAGGTCTCCAAGGT641420.18210179689747277No Hit
GTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGG640640.1818803516641155No Hit
GGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGT638320.18122169404695027No Hit
GTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCA634610.1801684096677765No Hit
GTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTC597460.16962137066877256No Hit
GTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGA595310.16901097675631338No Hit
GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAA583000.16551611672730293No Hit
GTGTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAGGTCTCCAAGG568570.16141937991019317No Hit
CTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTTAA552310.15680309850712978No Hit
CTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTCCGACC550180.15619838267757724No Hit
CTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACCCCCGCCACGCAGT542730.15408329679486982No Hit
GGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTC542510.15402083788289728No Hit
GTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAAT531290.15083543337229635No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG530400.15058275868295276No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT529680.15037834769831526No Hit
GGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTC525610.1492228578268228No Hit
GGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGG513340.1457393539636255No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAA511420.1451942580045922No Hit
GGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGC506230.14372079549033026No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC506040.14366685370271762No Hit
CCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACCCC505740.14358168245911865No Hit
CTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTT503440.14292870292486No Hit
ATTAGTGACGCGCATGAATGGATGAACGAGATTCCCACTGTCCCTACCTA493130.14000165118650926No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG492330.13977452787024536No Hit
AAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTC483360.1372279076866366No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG482170.1368900617536941No Hit
GGGGAATCCGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCG475180.13490557177783843No Hit
CATGAATGGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAA472690.13419865045596707No Hit
CCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAG470420.13355418804606833No Hit
CGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATG462590.1313312185881356No Hit
CCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCT458010.13003093760252488No Hit
CCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTC448470.12732249205607812No Hit
GTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTC447890.1271578276517868No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT447130.1269420605013361No Hit
GCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATGAACGAG446510.1267660399312316No Hit
AGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCC444200.12611022135551964No Hit
CTTGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCT443980.1260477624435471No Hit
CAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGC439320.12472476912630999No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG429930.12205890920166268No Hit
AACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCG421790.11974792945867771No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC418440.11879685057182271No Hit
CAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAA418250.11874290878421001No Hit
CACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACA412390.1170792304925771No Hit
GTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAGGTCTCCAAGGTG411940.11695147362717866No Hit
CCTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCC403010.1144162096093831No Hit
CGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA401170.11389382598197617No Hit
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA397900.11296545942674756No Hit
GTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTG395130.11217904494418386No Hit
CGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCG392160.11133584963255422No Hit
CGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTT391010.1110093598654249No Hit
CTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTC390920.11098380849234521No Hit
CTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAG387660.11005828097856989No Hit
GTTAGGACCCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAG384560.10917817812804735No Hit
GGTCAGTCGGTCCTGAGAGATGGGCGAGCGCCGTTCCGAAGGGACGGGCG382090.10847693488908264No Hit
GTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTCCGACCTGGGTATAGGG381210.10822709924119236No Hit
GGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTC380340.1079801026347554No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAAC379720.10780408206465088No Hit
CACGATCCTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTT377310.10711987307440594No Hit
CCCCGCCCCGCGGCGGGGCGCGGGACATGTGGCGTACGGAAGACCCGCTC376430.10687003742651567No Hit
CGAAGGCCCGCGGCGGGTGTTGACGCGATGTGATTTCTGCCCAGTGCTCT374270.1062568044726032No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCG367790.10441710561086576No Hit
GGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCAC367190.10424676312366785No Hit
AGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAA365920.10388620485909895No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGT363240.10312534174961493No Hit
GCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCG361910.10274774923632624No Hit
CGGGTGTTGACGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTG360150.10224807794054569No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC359670.10211180395078737No Hit
AAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAATGCGAGTGCCTAGTG358740.10184777309563062No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACA353630.10039702291299507No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)